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7. KESIMPULAN DAN SARAN

7.2 Saran

1. Perlu dilakukan penelitian lanjutan terhadap kemampuan pertumbuhan biji

yang berkecambah dari feses burung pemakan buah di habitatnya, sehingga dapat dipastikan bahwa burung pemakan buah membantu suksesi tumbuhan. 2. Perlu dilakukan sosialisasi pentingnya peranan burung pemakan buah kepada

masyarakat karena burung tersebut membantu menjaga kesetabilan ekosistem dan meningkatkan regenerasi dan penyebaran spesies tumbuhan buah.

3. Perlu dilakukan perbaikan dan menjaga jarak antara “patch” habitat supaya tidak terlalu jauh (kurang 200 m) dari jangkauan terbang burung, terutama burung-burung semak yang memiliki jarak terbang yang pendek.

4. Keberadaan beberapa spesies tumbuhan yang belum diketahui nilai

ekonomisnya perlu dilestarikan karena beberapa spesies dari tumbuhan tersebut seperti Saliara, Bungbrum, Kipapatong, Cecerenean, dan Harendong bermanfaat bagi kehidupan burung.

5. Perlu dilakukan penelitian lanjut pada burung-burung pemakan buah

pengguna kanopi pohon. Hal ini ditujukan agar diketahui potensi burung pemakan buah untuk menunjang reboisasi vegetasi hutan yang mengalami degradasi

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Lampiran 2. Jumlah curah hujan dan hari hujan tahun 2005 di lokasi penelitian Sumber data BMG, 2005 21 23 22 17 6 17 11 10 9 15 13 26 0 50 100 150 200 250 300 350 400 450

Jan Feb Mar Apr Mei Jun Jul Agst Sept Okt Nop Des

Jml hujan

Bulan

Lampiran 3. Lokasi pengamatan perilaku makan dan plot tumbuhan buah pakan burung Pycnonotus aurigaster dan Pycnonotus goiavier di semak kebun teh 5 tahun (KT5)

Keterangan Gambar:

Br : Tumbuhan Bungbrum (Polygonum chinenseL)

Bb : Bambu (Bambusp.)

H : Tumbuhan Harendong (Melastoma affine)

K : Tumbuhan Kaliandra (Caliandra haematochephala)

Kir : Tumbuhan Kirinyuh (Eupatorium inulifolium) PPt : Tumbuhan Kipapatong (Sambucus javanica BI) Tj : Tumbuhan Cecerenean (Breynia microphylla T&B)

Lampiran 4. Penentuan kadar protein dengan menggunakan metoda Kjeldall

Cara kerja

- Menimbang sebanyak 200-500 mg sampel yang telah dihaluskan lalu

memasukan ke dalam labu Kjeldahl.

- Menambahkan 10 ml asam sulfat pekat padat dan 5 gram katalis

(campuran K2SO4 dan CuSO4.5H2O perbandingan 8:1)

- Melakukan destruksi (dalam lemari asam) sampai cairan berwarna hijau jernih.

- Setelah dingin larutan tersebut diencerkan denga air suling sampai 100 ml dalam labu ukur.

- Mengambil 10 ml larutan di atas dan emmasukan ke dalam alat destilasi Kjeldahl lalu menambah 10 ml NaOH 30%.

- Destilasi dioperasikan selama 20 menit dan destilatnya ditampung dalam erlemeyer yang berisi 25 ml larutan HCl 0,1 N.

- Kelebihan HCl lalu dititrasi dengan NaOH 0,1 N dengan indikator

Tashiro. Perhitungan

% N = (((ml HCl sampel - ml HCl blangko) x N. HCl x 14.000))/mg sampel )x 100

% protein = % N x faktor konversi Faktor konversi kadar protein

Bahan Faktor

Konversi

1. Biji-bijian, makanan ternak, buah-buahan, anggur 6,25

2. Kenari 5,18

3. Kedelai 5,71

4. Kacang tanah 5,46

Lampiran 5. Penentuan kadar karbohidrat dengan metoda Anthorane

Cara kerja

1. Pipet ke dalam tabung reaksi 0,0 (blank0), 0,2, 0,4, 0,6, 0,8, dan 1,0 ml larutan glukosa standar. Menambahkan air sampai total volume masing- masing tabung reaksi 1,0 ml.

2. Menambahkan dengan cepat 5 ml pereaksi Anthrone ke dalam masing-

masing tabung.

3. Menutup tabung reaksi campuran merata.

4. Menempatkan dalam water bath 1000C selama 12 menit (meredam

dalam air mendidih).

5. Mendinginkan dengan cepat menggunakan air mengalir.

6. Memindahkan ke dalam kuvet, membaca absorbansnya pada 630 nm.

7. Membuat kurva hubungan antara Absorbans dengan mg glukosa.

Penetapan sampel

- Memasukan 1 ml sampel ke dalam tabung reaksi.

- Selanjutnya melakukan tahap (2) sampai (6) seperti pada pembuatan

kurva standar.

- Menentukan konsentrasi total karbohidrat dalam sampel

Perhitungan

Berat (gr) sampel = W

Absorbans glukosa standar = a (catatan 2)

Absorbans sampel = b

Total “available carbohidrate” (dinyatakan sebagai % glukosa) =

)

(

)

(

a w b × × 25 Catatan:

1. Warna hijau stabil selama 2 jam

2. Hubungan antara absorbans dengan kadar glukosa dengan kisaran 0-1,15 mg bersifat linier dan berbentuk garis lurus.

Lampiran 6. Penentuan kadar lemak dengan metoda Soxhlet

Cara kerja

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