BAB II. PENELAAHAN PUSTAKA
B. Saran
1. Perlu dilakukan penambahan replikasi dan pada konsentrasi yang berbeda
sehingga efisiensi ekstraksi dapat diketahui.
2. Perlu dilakukan penambahan interval waktu pengambilan sampel ikan nila
102
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Lampiran 1. Perhitungan Sensitivitas Alat
Sensitifitas alat ditentukan dengan menghitung LOD dari: a. Kurva baku 1 Konsentrasi Baku Deltametrin (µg/mL) Rasio AUC Deltametrin/DCB 0,155 0,2329 0,206 0,3141 0,258 0,4046 y = 1,66723 x - 0,02681 r = 0,99967
Menggunakan program powerfit didapatkan: POLYNOMIAL is: F(x) = 1,66723 x - 0,02681
Coefficient Std Dev, Min, Limit Max, Limit a0 -2,68051E-002 9,00928E-003 -1,41275E-001 8,76651E-002 a1 1,66723E+000 4,27843E-002 1,12362E+000 2,21084E+000
Dihitung menggunakan rumus: LOD = 3,3 � � = 3,3 x , , = 0,017814 µg/mL b. Kurva baku 2 c. Konsentrasi Baku Deltametrin (µg/mL) Rasio AUC Deltametrin/DCB 0,155 0,9775 0,206 1,3185 0,258 1,6780 y = 6,80134 x - 0,07868 r = 0,99995
Menggunakan program powerfit didapatkan: POLYNOMIAL is: F(x) = 6,80134 x - 0,07868
Coefficient Std Dev, Min, Limit Max, Limit a0 -7,86762E-002 1,38084E-002 -2,54123E-001 9,67710E-002 a1 6,80134E+000 6,55750E-002 5,96816E+000 7,63452E+000
Dihitung menggunakan rumus: LOD = 3,3 � � = 3,3 x ,
,
Lampiran 2. Kurva Adisi Deltametrin pada sampel ikan nila
Menggunakan powerfit maka diperoleh:
POLYNOMIAL is: F(x) = 0,02250 + 0,03796 x
Coefficient Std. Dev Min. Limit Max. Limit a0 2,25047E-002 1,50047E-002 -9,90658E-003 5,49159E-002 a1 3,79603E-002 4,11805E-004 3,70708E-002 3,88498E-002 Dihitung menggunakan rumus: LOQ = 3,3 x �
= 3,3 x ,
,
Lampiran 3. Uji Signifikansi Perbedaan Slope Kurva Baku dan Kurva Adisi
Baku Deltametrin Adisi Deltametrin b 1,30359 1,30001 SDb 0,0073 0,0141
n 7 15
- Uji signifikansi standar deviasi dengan uji F
F = �
� (S1
2 = standar deviasi slope baku; S22 = standar deviasi slope adisi
F = ,
, = 0,2680
Perhitungan Degrees of freedom (df) = n1-1 dan n2-1
n1-1 = 7-1 = 6 n2-1 = 15-1 = 14
α F hitung F tabel Kesimpulan 0,05 0,2680 3,501 Tidak signifikan
Uji t untuk melihat signifikansi slope antara kurva baku dan kurva adisi
Dari hasil perhitungan uji F diatas dapat dilihat SD antara kurva baku dan kurva adisi tidak berbeda signifikan, maka standar deviasi untuk uji t dihitung dengan persamaan: S2 = � − � + � − � � +� − Adisi y = 1.3x + 0.0225 R² = 0.9992 Baku y = 1.3036x + 0.0569 R = 0.9999 0 1 2 3 4 5 6 7 8 0 1 2 3 4 5 6 R as io A U C D el tam et ri n/ D C B Konsentrasi (µg/mL)
Kurva Baku vs Kurva Adisi
Adisi Baku
S2 = − , + − , + − S2 = , � − + , � − S2 = 1,55152 x 10-4 S = 0,0125 Perhitungan nilai t: t = | − | � √� + � t = | , − , | , √7+ t = , � − , � − t = 0,6257 perhitungan df: df = n1 + n2 -2 df = 7 + 15 -2 df = 20
α F hitung F tabel Kesimpulan 0,05 0,6257 2,09 Tidak signifikan
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BIOGRAFI PENULIS
Penulis skripsi dengan judul “Pengembangan Metode Analisis Deltametrin dalam Matriks Ikan Nila (Oreochromis niloticus) dan Aplikasinya pada Asesmen Resiko Deltametrin Melalui Asupan Ikan Nila” ini memiliki nama lengkap Oei Johanes Darma Hendra Sandjaja. Penulis dilahirkan di Semarang pada tanggal 19 Juli 1991 sebagai anak pertama dari tiga bersaudara, dari pasangan Oei Bharata Putra Sandjaja dan Jeni. Pendidikan formal yang pernah ditempuh penulis yaitu TK Pangudi Luhur Bernardus Semarang (1995 – 1997). Sekolah Dasar (SD) Pangudi Luhur Bernardus Semarang (1997 – 2000), Sekolah Dasar (SD) Santo Yoseph 1 Denpasar (2000 – 2003) Sekolah Menengah Pertama (SMP) Tegaljaya Denpasar (2003 – 2006), Sekolah Menengah Atas (SMA) Negeri 4 Denpasar (2006 – 2009) dan pada tahun 2009 melanjutkan pendidikan di Fakultas Farmasi Universitas Sanata Dharma Yogyakarta. Selama kuliah, penulis aktif dalam berbagai kegiatan dan kepanitiaan antara lain Panitia Kampanye Informasi Obat (KIO) 2010 sebagai Perkap, Panitia Pharmacy Performance and Event Cup (PPnEC) 2010 sebagai Perkap, Panitia Donor Darah JMKI 2010 sebagai Perkap, Panitia Seminar Kanker Serviks dan Kanker Paru-paru sebagai Perkap, Panitia Malam Keakraban JMKI sebagai Perkap, Panitia Inisiasi Tiga Hari Temu Akrab Farmasi (TITRASI) 2011 sebagai Perkap, Panitia Paingan Festival 2011 sebagai Keamanan, Panitia Pharmacy Performance (PP) 2011 sebagai Perkap, Panitia Pharmacy Days 2012 sebagai tentor, Panitia Pharmacy Performance and Event Cup (PPnEC) 2012 sebagai Ketua Eksternal, serta menjadi asisten praktikum Mikrobiologi 2010, Farmakognosi Fitokimia I 2011, Mikrobiologi 2011, Analisis Farmasi 2012, Validasi Metode Analisis 2012, Kimia Analisis 2013, Pharmaceutical Analysis 2013, Anatanomi dan Fisiologi Manusia 2014, Mikrobiologi 2014, Analisis Farmasi 2014, Validasi Metode Analisis 2014.