BOGOR
2012
DAFTAR PUSTAKA
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Lampiran 1. Diagram Alir Penelitian
Contoh Air Laut Hasil Sampling + Media
Inkubasi selama 1 minggu pada shaker inkubator pada suhu ruang, 150 rpm.
Isolasi Koloni Tunggal
(Inokulasi kultur pengayaan sebanyak 20 µL pada media padat)
Pemurnian Isolat Potensial (Metode direct pick-up colony)
Uji Manolitik
(Metode Pewarnaan Congo Red)
Analisis Gen 16S rRNA (Metode PCR)
Isolasi genom (Kit Instagene BioRad) Uji aktivitas Enzim mananase
(Metode spektrofotometer/DNS) Elektroforesis PENELITIAN TAHAP II PENELITIAN TAHAP I
Amplifikasi Gen 16S rRNA
Analisis Data 16S rRNA (Bioedit, RDP dan MEGA 5) Sekuensing
Lampiran 2 Hasil Contiq Bakteri Manolitik Laut Pari 3 GGCCAGGGGGGGGGGCTAATACATGCAAGTCGAGCGGACAGAAGGGAGCTT GCTCCCGGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCT GTAAGACTGGGATAACTCCGGGAAACCGGAGCTAATACCGGATAGTTCCTT GAACCGCATGGTTCAAGGATGAAAGACGGTTTCGGCTGTCACTTACAGATG GACCCGCGGCGCATTAGCTAGTTGGTGGGGTAATGGCTCACCAAGGCGACG ATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGG CCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAA GTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGC TCTGTTGTTAGGGAAGAACAAGTGCGAGAGTAACTGCTCGCACCTTGACGG TACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATAC GTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCG GTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTG GAAACTGGGAAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGC GGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCT GGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAG ATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTT TCGCCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGGAGT ACGGTCGCAAAGACTGAAAACTCAAAAGGAAATTGACGGGGGGCCCCCCAC AAACCGGTGGAACCATGTGGTTTAAATTCCAAACCAACCCCAAAAAAACCT TTACCAAGGTCTTTGACATCCTTCTGAACAACCCCTAAGAAGATAGGGGCT TTTCCCCTTCCGGGGAACAGAATTGAACAGGGTGGGTGCCTTGGT
Lampiran 3 Hasil Contiq Bakteri Manolitik Laut Pari 4
1 TTAGGCGGCT GGCTCCAAAA AGGTTACCCC ACCGACTTCG GGTGTTACAA
ACTCTCGTGG TGTGACGGGC GGTGTGTACA AGGCCCGGGA ACGTATTCAC 101 CGCGGCATGC TGATCCGCGA TTACTAGCGA TTCCAGCTTC ATGTAGGCGA GTTGCAGCCT ACAATCCGAA CTGAGAACGG TTTTATGAGA TTAGCTCCAC 201 CTCGCGGTCT TGCAGCTCTT TGTACCGTCC ATTGTAGCAC GTGTGTAGCC CAGGTCATAA GGGGCATGAT GATTTGACGT CATCCCCACC TTCCTCCGGT 301 TTGTCACCGG CAGTCACCTT AGAGTGCCCA ACTAAATGAT GGCAACTAAG ATCAAGGGTT GGGCTCGTTG CGGGACTTAA CCCAACATCT CACGACACGA 401 GCTGACGACA ACCATGCACC ACCTGTCACT CTGCTCCCGA AGGAGAAGCC CTATCTCTAG GGTTGTCAGA GGATGTCAAG ACCTGGTAAG GTTCTTCGCG 501 TTGCTTCGAA TTAAACCACA TGCTCCACCG CTTGTGCGGG CCCCCGTCAA TTCCTTTGAG TTTCAGCCTT GCGGCCGTAC TCCCCAGGCG GAGTGCTTAA 601 TGCGTTAACT TCAGCACTAA AGGGCGGAAA CCCCTCTAAC ACTTTAGCAC TTCATCGTTT TACGGCGTGG GACTACCCAG GGTATCTAAT CCCCGTTTGC 701 TCCCCCACGC TTTCGCGCCT CAGTGTCAGT TACAGACCAG AAAGTCGCCT TCGGCCACTG GTGTTCCTCC ATATCTCTAC GCATTTCACC GCTACACATG 801 GAATTCCACT TTCCTCTTCT GCACTCAAGT CTCCCAGTTT CCAATGACCC TCCACGGTTG AGCCGTGGGC TTTCACATCA GACTTAAGAA ACCACCTGCG 901 CGCGCTTTAC GCCCAATAAT TCCGGATAAC GCTTGCCACC TACGTATTAC CGCGGCTGCT GGCACGTAGT TAGCCGTGGC TTTCTGGTTA GGTACCGTCA 1001 AGGTGCCAGC TTATTCAACT AGCACTTGTT CTTCCCTAAC AACAGAGTTT TACGACCCGA AAGCCTTCAT CACTCACGCG GCGTTGCTCC GTCAGACTTT 1101 CGTCCATTGC GGAAGATTCC CTACTGCTGC CTCCCGTAGG AGTCTGGGCC GTGTCTCAGT CCCAGTGTGG CCGATCACCC TCTCAGGTCG GCTACGCATC 1201 GTTGCCTTGG TGAGCCGTTA CCTCACCAAC TAGCTAATGC GACGCGGGTC CATCCATAAG TGACAGCCGA AGCCGCCTTT CAATTTCGAA CCATGCGGTT 1301 CAAAATGTTA TCCGGTATTA GCCCCGGTTT CCCGGAGTTA TCCCAGTCTT ATGGGCAGGT TACCCACGTG TTACTCACCC GTCCGCCGCT AACTTCATAA 1401 GAGCAAGCTC TTAATCCATT CGCTCGACTT GCAT
Lampiran 4 Hasil Contiq Bakteri Manolitik Laut Pari 5
1 GAAGGGGGGG TGCTATACAT GCAAGTCGAG CGAATGGATT AAGAGCTTGC TCTTATGAAG TTAGCGGCGG ACGGGTGAGT AACACGTGGG TAACCTGCCC 101 ATAAGACTGG GATAACTCCG GGAAACCGGG GCTAATACCG GATAACATTT TGAACCGCAT GGTTCGAAAT TGAAAGGCGG CTTCGGCTGT CACTTATGGA 201 TGGACCCGCG TCGCATTAGC TAGTTGGTGA GGTAACGGCT CACCAAGGCA ACGATGCGTA GCCGACCTGA GAGGGTGATC GGCCACACTG GGACTGAGAC 301 ACGGCCCAGA CTCCTACGGG AGGCAGCAGT AGGGAATCTT CCGCAATGGA CGAAAGTCTG ACGGAGCAAC GCCGCGTGAG TGATGAAGGC TTTCGGGTCG 401 TAAAACTCTG TTGTTAGGGA AGAACAAGTG CTAGTTGAAT AAGCTGGCAC CTTGACGGTA CCTAACCAGA AAGCCACGGC TAACTACGTG CCAGCAGCCG 501 CGGTAATACG TAGGTGGCAA GCGTTATCCG GAATTATTGG GCGTAAAGCG CGCGCAGGTG GTTTCTTAAG TCTGATGTGA AAGCCCACGG CTCAACCGTG 601 GAGGGTCATT GGAAACTGGG AGACTTGAGT GCAGAAGAGG AAAGTGGAAT TCCATGTGTA GCGGTGAAAT GCGTAGAGAT ATGGAGGAAC ACCAGTGGCG 701 AAGGCGGACT TTTTTGGTCT GTAAACTGAC AATTGAGGCG GGGAAAGCGT GGGGAGCAAA CAGGATTAGA TACCCTGGTA GTCCACGCCG TAAACGATGA 801 GTGCTAAGTG TTAGAGGGTT TCCGCCCTTT AGTGCTGAAG TTACGCATTA AGCACTCCGC CTGGGGAGTA CGGCCGCAAG GCTGAAACTC AAAGGAATTG 901 ACGGGGGCCC GCACAAGCGG TGGAGCATGT GGTTTAATTC GAAGCAACGC GAAGAACCTT ACCAGGTCTT GACATCCTCT GACAACCCTA GAGATAGGGC 1001 TTCTCCTTCG GGAGCAGAGT GACAGGTGGT GCATGGTTGT CGTCAGCTCG TGTCGTGAGA TGTTGGGTTA AGTCCCGCAA CGAGCGCAAC CCTTGATCTT 1101 AGTTGCCATC ATTTAGTTGG GCACTCTAAG GTGACTGCCG GTGACAAACC GGAGGAAGGT GGGGATGACG TCAAATCATC ATGCCCCTTA TGACCTGGGC 1201 TACACACGTG CTACAATGGA CGGTACAAAG AGCTGCAAGA CCGCGAGGTG GAGCTAATCT CATAAAACCG TTCTCAGTTC GGATTGTAGG CTGCAACTCG 1301 CCTACATGAA GCTGGAATCG CTAGTAATCG CGGATCAGCA TGCCGCGGTG AATACGTTCC CGGGCCTTGT ACACACCGCC CGTCACACCA CGAGAGTTTG 1401 TAACACCCGA AGTCGGTGGG GTAACCTTTT TGGAGCCAGC CGCCTAAGGT
Lampiran 5 Kromatogram Bakteri Manolitik Laut Pari 3
Screen clipping taken: 9/21/2012, 2:39 AM