FLAVONOID PADA EKSTRAK TEMU IRENG DAN EKSTRAK KI URAT
6 SIMPULAN DAN SARAN
6.1 Simpulan
Beberapa hal yang dapat disimpulkan dari fakta-fakta yang diperoleh melalui penelitian ini, yaitu:
a. Ekstrak etanol rimpang Temu Ireng dan ekstrak etanol daun Ki Urat ditentukan sebagai kandidat ekstrak bahan aktif antiphotoaging karena memiliki aktivitas penghambatan pembentukan MMP-1 selama paparan UV dan memiliki aktivitas antioksidan.
b. Ekstrak etanol daun Ki Urat berpotensi sebagai bahan aktif antiphotoaging yang dapat menghambat pembentukan MMP-1 dan menghambat penurunan pembentukan prokolagen tipe I melalui mekanisme aktivitas antioksidan.
c. Keberadaan senyawa fenol, khususnya flavonoid, dalam ekstrak tersebut sebagai antioksidan berperan penting dalam mencegah photoaging dengan menghambat pembentukan MMP-1 dan menghambat penurunan pembentukan prokolagen tipe I akibat paparan UV.
6.2 Saran
Berdasarkan hasil penelitian ini maka diajukan beberapa saran yang perlu untuk ditindaklanjuti,
a. Perlu dilakukan penelitian dan pengembangan ekstrak etanol daun Ki Urat sebagai bahan aktif antiphotoaging dalam formula produk kosmetik komersial melalui suatu rangkaian uji klinis.
b. Perlu dilakukan isolasi terhadap senyawa antioksidan pada ekstrak etanol daun Ki Urat yang berperan langsung dalam aktivitas antiphotoaging.
PUSTAKA
Abeyama K, Eng W, Jester JV, Arie AV, Edelbaum D, Cockerell CJ, Bergstresser PR, Takashima A. 2000. A role for NF-kB–dependent gene transactivation in Sunburn.The Journal of Clinical Investigation 105(12) :1751–1759.
Afaq H, Mukhtar H. 2006. Botanical antioxidants in the prevention of photocarcinogenesis and photoaging. Experimental Dermatology 15: 678–684.
Agius CJ, Baron BY, Brincat MP. 2007. Skin ageing. Menopause International 13: 60–64.
Almeida IF, Valentao P, Andrade PB, Seabra RM, Pereira TM, Amaral MH, Costa PC, Bahia MF. 2008. Oak leaf extract as topical antioxidant: free radical scavenging and iron chelating activities and in vivo skin irritation potential. BioFactors 33: 267–279.
Ameh SJ, Bodozie OO, Inyang US, Abubakar MS, Garba M. 2010.quality control tests onandrographis paniculata nees (Family: Acanthaceae)– an indian ‘wonder’ plant grown in Nigeria. Tropical Journal of Pharmaceutical Research 9 (4): 387-394.
Audonneau JLC, Jeanmaire C, Pauly G. 1999. A histological study of human wrinkle structures: comparison between sun-exposed areas of the face, with or without wrinkles, and sun-protected areas. British Journal of Dermatology 140: 1038–1047.
Baiano A.Gambacorta G, Terracone C, Previtali MA, Lamacchia C, Notte EL. 2009. Changes in phenolic content and antioxidant activity of Italian extra-virgin olive oils during storage. Journal of Food Science 74(2): 117-183.
Baron E, Kirkland EB, Domingo DS. 2008. Advances in Photoprotection. Dermatology Nursing 20(4): 265-272.
Brodsky B, Bel Bruno KC, Hardt TA, Eikenberry EF. 1994. Collagen fibril structure in lamprey. Journal of Molecular Biology 243(1):38-47. Chang C, Yang M, Wen H, Chern J. 2002. Estimation of total flavonoid
content in propolis by two complementary colorimetric methods. Journal of Food Drug Analysis 10: 178-182.
Chen KC, Chang HH, Ko WS, Wu CL, Chiu WT, Hsieh CL, Peng RY. 2009. UV-induced damages eliminated by arbutin and ursolic acid in cell model of human dermal fibroblast WS-1 cells. Egyptian Dermatology Online Journal 5(1):1-15.
Choquenet B, Couteau C, Laurence EP, Coiffard JM. 2008. Quercetin and rutin as potential sunscreen agents: determination of efficacy by an in vitromethod. Journal of Natural Product 71 (6): 1117–1118.
Chung JH, Youn SH, Kwon OS, Eun HC,Kim KH, Park KC, Cho KH, Youn JI. 1996. Enhanced proliferation and collagen synthesis of human dermal fibroblasts in chronically photodamaged skin. Photodermatology Photoimmunology Photomedical 12: 84–89.
Chung JH, Youn SH, Kwon OS, Cho KH, Youn KI, Eun HC. 1997. Regulations of collagen synthesis by ascorbic acid, transforming
growth factor-beta and interferon-gamma in human dermal fibroblasts cultured in three-dimensional collagen gel are photoaging- and aging- independent. Journal Dermatology Science 15:188–200.
Chung JH, Seo JY, Choi HR, Lee MK, Youn CS, Rhie Gi-eun, Cho KH, Kim KH, Park KC, Eun HC. 2001. Modulation of skin collagen metabolism in aged and photoaged human skin in vivo. Journal of Investigative Dermatology 117:1218–1224.
Chung JH, Yano K, Lee MK, Youn CS, Seo JY, Kim KH, Cho KH, Eun HC, Detmar M. 2002a. Differential effects of photoaging vs intrinsic aging on the vascularization of human skin. Archives of Dermatology 138:1437-1442.
Chung JH, Seo JY, Lee MK, Eun MK, Lee JH, Kang S, Fisher GJ, Voorhees JJ. 2002b. Ultraviolet modulation of human macrophage metalloelastase in human skin in vivo. Journal of Investigative Dermatology 119:507–512.
Chung JH. 2003. Photoaging in Asians. Photodermatology Photoimmunology & Photomedicine 19: 109-121.
Chung JH, Han JH, Eun JH, Seo JY, Cho KH, Kim KH, Youn JI, Eun HC. 2003. Dual mechanisms of green tea extract (EGCG)-induced cell survival in human epidermal keratinocytes. The FASEB Journal Express 17:1913–1915.
Cross SE, Innes B, Roberts MS, Tsuzuki T, Robertson TA, McCormick P. 2007. Human skin penetration of sunscreen nanoparticles: in-vitro assessment of a novel micronized zinc oxide formulation. Skin Pharmacology and Physiology 20:148–154.
Dai J, Mumper RJ. 2010. Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules 15: 7313-7352
Dong KK, Damaghi N, Picart SD, Markova NG, Obayashi K, Okano Y, Masaki H, Susanne Grether-Beck, Krutmann J, Smiles KA, Yarosh DB. 2008. UV-induced DNA damage initiates release of MMP-1 in human skin. Experimental Dermatology 17: 1037–1044.
Dawidowicz AL, Olszowy M. 2012. Mechanism change in estimating of antioxidant activity of phenolic compounds. Talanta 97: 312–317 Edlich RF, Winters KL, Lim HW, Cox MJ, Becker DG, Horowitz JH, Nichter
LS, Britt WB. Long. 2004. Photoprotection by sunscreens with topical antioxidants and systemic antioxidants to reduce sun exposure. Journal of Long-Term Effects of Medical Implants 14(4):317–340.
El-Sayed MM, El-Hashash MM, El-Wakil EA, Ghareeb MA. 2009. Total phenolic contents and antioxdant activities of ficus sycomorus and Azadirachta indica. Pharmacology online 3: 590-602
Evelson P, Ordonez CP, Liesuy SL, Boveris A. 1997. Oxidative stress and in vivo chemiluminescence in mouse skin exposed to uv a radiation. Journal of Photochemsitry and Photobiology B; Biology 38: 215-219. Exposito JY, Cluzel C, Garrone R, Lethias C. 2002. Evolution of collagens.
The Anatomical Record 268(3): 302–316.
Fisher, G. J., S.C. Datta, H.S. Talwar, Z.Q. Wang, J. Varani, S. Kang , J.J. Voorhees. 1996. Molecular basis of sun-induced premature skin ageing and retinoid antagonism. Nature 379(6563): 335-339.
Fisher GJ, Zeng Quan W, Subhash CD, James V, Sewon Kang, Voorhees JJ. 1997. Pathophysiology of premature skin aging induced by ultraviolet light. The New England Journal of Medicine 337(20): 1419-1428. Fisher GJ, Harvinder S. Talwar, Jiayuh Lin, John J. Voorhees. 1999. Molecular
mechanisms of photoaging in human skin in vivo and their prevention by all-trans retinoic acid. Photochemistry and Photobiology 69(2): 154- 157.
Fisher GJ, Kang S, Varani J, Zsuzsanna Bata-Csorgo, Wan Y, Datta S, Voorhees JJ. 2002. Mechanisms of photoaging and chronological skin aging. Archive Dermatology 138: 1462-1469.
Fisher GJ, Quan T, Purohit T, Shao Y, Cho MK, He T, Varani J, Kang S, Voorhees JJ. 2009. Collagen fragmentation promotes oxidative stress and elevates matrix metalloproteinase-1 in fibroblast in aged human skin. The American Journal of Pathology 174(1): 101-114.
Galicka A, Nazaruk J. 2007. Stimulation of collagen biosyntesis by flavonoid glycosides in skin fibroblast of osteogenesis imperfecta tipe i and potential mechanism of their action. International Journal of Molecular Medicine 20: 889-895.
Gilchr est BA. 1989. Skin aging and phot oaging: an over view . Jour nal of t he Amer ican Academy of Der mat ology 21:610-613.
Gonzalez H. 2006. Studie of Sunscreens: Percutaneous Absorption of Benzophenone-3 and Photostability. Sweden: Departement of Dermatology and Venereology. Institute of Clinical Sciences. The Sahlgrenska Academy at Goterborg University.
Halilu M, Abubakar A, Garba MK, Isah AA. 2012. Antimicrobial and preliminary phytochemical studies of methanol extract of root bark of Crossopteryx febrifuga(Rubiaceae). Journal of Applied Pharmaceutical Science 2 (12): 66-70.
Helfrich YR, Sachs DL, Voorhees JJ. 2008. Overview of skin aging and photoaging. Dermatologi Nursing 20(3): 177-183.
Herrling T, Jung K, Chatelain E, Langenauer M. 2006. Radical skin/sun protection factor rsf –protection against uv-induced free radicals in skin. SÖFW (Seifen, Öle, Fette, Wachse)-Journal 132: 24-30.
Ho JN, Lee HY,Park JS, Jun WJ, Kim HK, Hong BS, Shin DH, Cho HY. 2005. Protective effects of aucubin isolated fromEucommia ulmoides against UVB-induced oxidative stress in human skin fibroblasts. Biological & Pharmaceutical Bulletin 28(7): 1244-1248.
Ho TY. 2001. Sunscreens: is looking at sun protection factor enough?. Hong Kong Dermatology & Venereology Bulletin 9(3): 101-108.
Holasova M, Fiedlerova H, Smrcinova, Orsak M, Lachman J, Vavreinova S. 2002. Buckwheat, the source of antioxidant activity in functional foods. Food Research International. 35: 207-211.
Huang CC, Wu WB, Fang JY, Chiang HS, Chen SK, Chen BH, Chen YT, Hung CF. 2007. (-)-Epicatechin-3-gallate, a green tea polyphenol is a potent agent against UVB-induced damage in HaCaT keratinocytes. Molecules 12: 1845-1858.
Isfahlan AJ, Mahmoodzadeh A, Hassanzadeh A, Heidari R, Jamei R. 2010. Antioxidant and antiradical activities of phenolic extracts from Iranian
almond (Prunus amygdalus L.) Hulls and Shells. Turkish Journal of Biology 34: 165-173.
James CN, Middleton JG. 1981. Determination of sun protection factor in the hairless mouse. International Journal of Cosmetic Science. 3: 153-158. Jang S, Chun J, Shin EM, Kim H, Kim YS. 2011. Inhibitory effects of
curcuminoids from Curcuma longa on matrix metalloproteinase-1 expression in keratinocytes and fibroblasts. Journal of Pharmaceutical Investigation 40 (3): 7-14.
Javanmardi J, Stushno C, Locke E, Vivanco JM. 2003. Antioxidant activity and total phenolic content of Iranian ocimumaccessions. Food Chemistry 83: 547–550.
Jenkins G. 2002. Molecular mechanisms of skin ageing. Mechanisms of Ageing and Development 123 (7): 801-810.
Jeong JM, Kang SK, Lee IH, Lee JY, Jung H, Choi CH. 2007. Antioxidant and chemosensitizing effects of flavonoids with hydroxy and/or methoxy groups and structure-activity relationship. Journal of. Pharmacy & Pharmaceutical Sciences 10(4): 537-546.
Jirovetz L, Buchbaner G, Pushmann C, Shafi MP, Nambiar MKG. 2000. Essential oil analysis ofCurcuma aeruginosaRoxb. Leaves from South India. Journal of Essential Oil Research 12 (1): 47-49.
Kadler KE, Baldock C, Bella J, Handford RPB. 2007. Collagens at a glance. Journal of Cell Science 120: 1955-1958.
Katalinic V, Milos M, Kulisic T, Jukic M. 2006.Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chemistry 94: 550–557.
Kim HH, Shin CM, Park CH, Kim KH, Cho KH, Eun HC, Chung JH. 2005. Eicosapentaenoic acid inhibits UV-induced MMP-1 expression in human dermal fibroblasts. Journal of Lipid Research 46: 1712-1720. Kim C, Ryu HC, Kim JH. 2010. Low-dose UVB Irradiation stimulates matrix
metalloproteinase-1 expression via A Blt2-linked pathway in Hacat cells. Experimental and Molecular Medicine 42(12): 833-841.
Kim MS, Oh GH, Kim MJ, Hwang JK. 2013. Fucosterol inhibits matrix metalloproteinase expression and promotes type-1 procollagen production in UVB-induced HaCaT cells. Photochemistry and Photobiology 89(4): 911-918.
Korać RR, Khambholja KM. 2011. Potential of herbs in skin protection from ultraviolet radiation. Pharmacognosy Review 5(10): 164–173.
Kotch FW, Raines RT. 2006. Self-assembly of synthetic collagen triple helices. Proceeding of National Academy of Science U.S.A. 103: 3028- 3033.
Kumar KS, Ganesan K, Rao PVS. 2012. Antioxidant potential of solvent extracts of Kappaphycus alverezii (Doty). An edible seaweed. Food Chemistry 107: 289-295.
Laga AC, Murphy GF.2009. The translational basis of human cutaneous photoaging on models, methods, and meaning. The American Journal of Pathology 174(2): 357-360.
Lee YM, Kim YK, Kim KH, Park SJ, Kim SJ, Chung JH. 2009a. A novel role for the TRPV1 channel in UV-induced matrix metalloproteinase
(MMP)-1 expression in HaCaT cells. Journal of Cellular Physiology 219(3): 766–775.
Lee YL, Lee MH, Chang HJ, Huang PY, Huang IJ, Heng KT, Leu SJ. 2009b. Taiwanese native plants inhibit matrix metalloproteinase-9 activity after ultraviolet B irradiation. Molecules 14:1062-1071.
Li P, Huo L, Su W, Lu R, Deng C, Liu L, Deng Y, Guo N, Lu C, He C. 2011. Free radical-scavenging capacity, antioxidant activity and phenolic content of pouzolzia zeylanica. Journal of the Serbian Chemical Society. 76(5) 709–717.
Lim SH, Jung SK, Byun S, Lee EJ, Hwang JA, Seo SG, Kim YA, Yu JG, Lee KW, Lee HJ. 2013. Luteolin suppresses UVB-induced photoageing by targeting JNK1 and p90RSK2. Journal of Cellular and Molecular Medicine 17(5): 672-680.
Lin, Fu-Hsiung., Jing-Yi Lin, Ravindra D. Gupta, Joshua A. Tournas, James A. Burch, M. Angelica Selim, Nancy A. Monteiro-Riviere, James M. Grichnik, Jan Zielinski, Sheldon R. Pinnell. 2005. Ferulic acid stabilizes a solution of vitamins C and E and doubles its photoprotection of skin. The Journal of Investigative Dermatology 125: 826–832.
Litwinienko G, Mulder P. 2009. Comment on ‘‘temperature and solvent effects on radical scavenging ability of phenols’’. The Journal of Physical Chemistry A. 113 (50): 14014–14016.
Liu J, Chen X, Yang W, Zhan S, Wang F, Tang Z. 2010. Chemical fingerprinting of wild germplasm resource of Ophiopogon japonicus from Sichuan basin, China by RP-HPLC coupled with hierarchical cluster analysis. Analytical Letters 43: 2411–2423.
Lopez M, Martinez F, Valle CD, Ferrit M, Luque R. 2003.Study of phenolic compounds as natural antioxidants by a fluorescence method. Talanta 60: 609-616.
Lou Y, Zhang H, He H, Peng K, Kang N, Wei X, Li X, Chen L, Yao X, Qiu F. 2010. Isolation and identification of phase 1 metabolites of curcumol in rats. Drug Metabolim and Disposition 38(11):2014-2022.
Mao LF, Huo WM, Jun L, Jing S. 2012. Study on the antioxidant effects of Β- elemene. Acta Metallurgica Sinica 17(7): 727-730.
Masnec IS, Pojube S. 2008. Photoaging. Collagen Antropology 2: 177-180. McDonald S, Prenzler PD, Autolovich M, Robards K. 2001. Phenolic content
and antioxidant activity of olive extracts. Food Chemistry 73:73-84. Moon, Hyung-in, Jin Ho Chung, Joong-Ku Lee, Ok-Pyo Zee. 2004.
Triterpenoid saponin from Viola hondoensis W. Becker et H Boss and their effect on MMP-1 and type I procollagen expression. Archives Pharmacal Research 27(7): 730-733.
Mojab F, Kamalinejad M, Ghaderi N, Vahidipour HR. 2003. Phytochemical screening of some species of iranian plants. Iranian Journal of Pharmaceutical Research 2: 77-82.
Müller HF, Champ C, Kandzia C, Jung K, Seifert M, Herrling T. 2008. Strategy for efficient prevention from photo-ageing. SÖFW (Seifen, Öle, Fette, Wachse)-Journal 114: 23-32.
Nobre CP, Moura FNRFM. Moura. 2005. Standardization of extracts from Momordica charantia L. (Cucurbitaceae) by total flavonoids content determination. Acta Farmaceutica Bonaerense 24 (4): 562-566.
Nepote V, Nelson RG, Carlos AG. 2005. Optimization of extraction of phenolic antioxidants from peanut skins. Journal of the Science of Food and Agriculture 85:33–38.
Neto AC, Haidar M, Simões R, Simões M, Soares-Jr J, Baracat E.2009. Effects of isoflavones on the skin of postmenopausal women: a pilot study. Clinics 64(6): 505-510.
Nichols JA, Katiyar SK. 2010. Skin photoprotection by natural polyphenols: anti-inflammatory, anti-oxidant and DNA repair mechanisms. Archives of Dermatological Research 302(2): 71-76.
Nishigori C, Yukari H, Yaeno A, Yoshiki M. 2003. Photoaging and oxidative stress.Experimental Dermatology 12 Supl 2: 18–21.
Oliveira SL, Mansanares AM, Silva ECD, Barja PR. 2008. In vitro determination of the sun protection factor of sunscreens through photoacoustic spectroscopy: a new approach. The European Physical Journal Special Topic 153: 475-478.
Orcic DZ, Dukic NMM. Franciskovic MM, Petrovic S, Jovin ED. 2011. Antioxidant activity relationship of phenolic compounds in Hypericum PerforatumL. Chemistry Central Journal 5: 34-41
Ottani V, Raspanti M, Ruggeri A. 2001. Collagen structure and functional implications. Micron 32: 251–260.
Perron NR, Brumaghim JL. 2009. A Review of the antioxidant mechanisms of polyphenol compounds related to iron binding. Cell Biochemistry and Biophysics 53:75–100
Philips N, Arena R, Yarlagadda S. 2009. Inhibition of ultraviolet radition mediated extracellular matrix remodeling in fibroblasts by transforming growth factor-β. BIOS80(1): 20-24.
Pinnell SR. 2003.Cutaneous photodamage, oxidative stress, and topical antioxidant protection. Journal of The American Academy of Dermatology 48(1): 1-19.
Pissavini M, Ferrero L, Alard V, Heinrich U, Tronnier H, Kockott D, Lutz D, Tournier V, Zambonin M, Meloni M. 2003. Determination of the in vitro SPF. Cosmetics & Toiletries 118(10): 63-72.
Pluemsamran T, Onkoksoong T, Panich U. 2012. Caffeic acid and ferulic acid inhibit UVA-induced matrix metalloproteinase-1 through regulation of antioxidant defense system in keratinocyte HaCaT cells. Photochemistry and Photobiology 88(4):961-968.
Pourmorad F, Hosseinimehr SJ, Shahabimajd N.2006. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. African Journal of Biotechnology 5 (11): 1142-114.
Prockop DJ, Kivirikko KL. 1995. Collagens: Molecular biology, diseases, and potentials for therapy. Annual Review of Biochemistry 64: 403-434. Ramamoorthy PK, Bono A. 2007. Antioxidant activity, total phenolic and
flavonoid content of morinda citrifolia fruit extracts from various extraction processes. Journal of Engineering Science and Technology 2(1): 70-80.
Quan T, He T, Kang S, Voorhe Es JJ, Fisher GJ. 2004. Solar ultraviolet irradiation reduces collagen in photoaged human skin by blocking transforming growth factor-β type II receptor/smad signaling. American Journal of Pathology 163 : 741-751.
Que F, Mao L, Zhu C, Xie G. 2006. Antioxidant properties of Chinese yellow wine, its concentrate and volatiles. Lebensmittel-Wissenschaft und Technologie (LWT) 39:111-117.
Rios JL, Bas E, Recio MC. 2005. Effects of natural products on contact dermatitis. Current Medicinal Chemistry 4: 65-80.
Savithramma N, Rao ML, Suhrulatha D. 2011. Screening of medicinal plants for secondary metabolites. Middle-East Journal of Scientific Research 8 (3): 579-584
Seal T, Chaudhuri K, Pillai B. 2012. Antioxidant activity of some selected wild edible fruits of north-eastern region in India and effect of solvent extraction system. Global Journal of Environmental Research 6 (3): 84-90
Seo JY, Chung J. 2006. Thermal aging: a new concept of skin aging. Journal of Dermatological Science Supplement 2: S13—S22
Shin MH , Rhie G, Park CH, Kim KH, Cho KH, Eun HC, Chung JH. 2005a. Modulation of collagen metabolism by the topical application of dehydroepiandrosterone to human skin. The Journal of Investigative Dermatology 124: 315-323.
Shin MH, Rhie G, Kim YK, Park CH, Kim KH, Cho KH, Eun HC, Chung JH. 2005b. H2O2 accumulation by catalase reduction changes MAP Kinase
signaling in aged human skin in vivo. The Journal of Investigative Dermatology 125: 221–229.
Sies H, Stahl W. 2004. Nutritional protection againt skin demage from sunlight. Annual Review of Nutrition 24:173–200.
Silva S, Gomes L, Leitao L, Coelho AV, Boas LV. 2006. Phenolic compounds and antioxidant activity of Olea europaea L. fruits and leaves. Food Science and Technology Internationa; 12(5):385–396.
Sisa M, Bonnet SL, Ferreira D, Westhuizen JHVD. 2010. Photochemistry of flavonoids. Molecules 15: 5196-5245.
Stef DS, Gergen I, Trasca TI, Hermanescu M, Lavinia S, Ramona B, Heghedus MG. 2009. Total antioxidant and radical scavenging capacities for different medicinal herbs. Romanian Biotechnological Letters 14(5): 4704-4709.
Sultana B, Anwar F, Ashraf M. 2009. Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules 14: 2167-2180.
Svobodova A, Walterova D, Vostalova J. 2006. Ultraviolet light induced alteration to the skin. Biomedical Paper Medical Faculty University Palacky Olomouc Czech Republic 150(1):25–38.
Svobodová A, Psotová J, Walterová D. 2003. Natural phenolics in the prevention of UV-induced skin damage. A Review. Biomedical Papers 147(2): 137–145
Talukdar, A. Das. , M. Dutta Choudhury, M.Chakraborty , B.K. Dutta. 2010. Phytochemical screening and TLC profiling of plant extracts of
Cyathea gigantea (Wall. Ex. Hook.) Haltt.andCyathea brunoniana. Wall.ex. Hook. (Cl. & Bak.). Assam University Journal of Science &Technology : Biological and Environmental Sciences 5(10): 70-74 Tanaka S, Sato T, Akimoto N, Yano M, Ito A. 2004. Prevention of UVB-
induced photoinflammation and photoaging by a polymethoxy flavonoid, nobiletin, in human keratinocytes in vivo and in vitro. Biochemical Pharmacology 68(2): 433-439.
Thamaraiselvi PL, Jayanthi P. 2012. Preliminary studies on phytochemicals and antimicrobial activity of solvent extracts of Eichhornia crassipes (Mart.) Solms. Asian Journal of Plant Science & Research 2 (2):115- 122.
Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H.2011. Phytochemical screening and Extraction: a review. Internationale Pharmaceutica Sciencia 1(1): 98-106.
Traikovich SS. 1999. Use of topical ascorbic acid and its effects on photodamaged skin topography. Archives Otolaryngology - Head Neck Surgery 125:1091-1098.
Tsuchiya S, Honda MJ, Shinohara Y, Saito M, Ueda M. 2008. Collagen type I matrix affects molecular and cellular behavior of purified porcine dental follicle cells. Cell and Tissue Research 331(2): 447-459.
Uitto J, Olsen DR, Fazio MD. 1998. Extracellular matrix of the skin: 50 Years of Progress. The Journal of Investigative Dermatology 92: 61-77. Varani J, Perone P, Deming MO, Warner RL, Aslam MN, Bhagavathula N,
Dame MK, Voorhees JJ. 2009. Impaired keratinocyte function on matrix metalloproteinase-1 (MMP-1) damaged collagen. Archive Dermatology Research 301: 497–506.
Velioglu YS, Maza G, Gao L, Oomah BD. 1998. Antioxidant activity and total phenolics in selected fruits, vegetable and grain products. Journal of Agricultural and Food Chemistry. 46: 4113-4117.
Vijayalakshmi R, Ravindhran R. 2012. Preliminary comparative phytochemical screening of root extracts of Diospyrus ferrea (Wild.) Bakh and Aerva lanata (L.) Juss. Ex Schultes . Asian Journal of Plant Science & Research, 2(5):581-587.
Villano D, Pachon MSF, Troncoso AM, Parrilla MCG. 2006. Influence of ethnological practices on the antioxidant activity of wines. Food Chemistry 95: 394-404.
Wang H, Fang Y, Wang Y, Wang Z, Zou Q, Shi Y, Chen J, Peng D. 2012. Inhibitory effect of curcumol on Jak2-STAT signal pathway molecules of fibroblast-like synoviocytes in patients with rheumatoid arthritis. Evidence-Based Complementary and Alternative Medicine vol. 2012: 1-8.
Wertz K, Seifert N, Hunziker PB, Riss G, Wyss A, Lankin C, Goralczyk R. 2004. Beta-carotene inhibits UVA-induced matrix metalloprotease 1 and 10 expression in keratinocytes by a singlet oxygen-dependent mechanism. Free Radical Biology & Medecine 37:654-70.
Wu YB, Zheng LJ, Yi J, Wu JG, Chen TQ, Wu JZ. 2013. Quantitative and chemical fingerprint analysis for the quality evaluation of Receptaculum nelumbinis by RP-HPLCcoupled with hierarchical
clustering analysis. International Journal of Molecular Sciences 14: 1999-2010.
Yaar M, Eller MS, Gilchres BA. 2002. Fifty years of skin aging. Journal of Investigative Dematology Symposium Proceedings 7(1): 51-58.
Yadav RNS, Agarwala M. 2011.Phytochemical analysis of some medicinal plants. Journal of Phytology 3(12): 10-14.
Yasui H, Sakurai H. 2003. Age-dependent generation of reactive oxygen species in the skin of live hairless rats exposed to UVA light. Experimental Dermatology 12: 655–661.
Yu SM, Kim EK, Lee JH, Lee KR, Hong J. 2011. Development of fingerprints for quality control of Acorusspecies by gas chromatography/mass spectrometri. Bulletin of the Korean Chemical Society 32 (5): 1547- 1553.
Lampiran 2. Penentuan Konsentrasi Larutan Uji dan Dosis Paparan UV