420 Proceeding of the 2nd International Seminar on Animal Industry | Jakarta, 5-6 July 2012
Blood Metabolite Statues of Local Sheep Fed With
Indigofera
sp.
Dewi Apri Astuti*, Sri Rahayu, Budi Satoto, Rudy Priyanto, Lilis Khotidjah, Tuti Suryati, & M. Baihaqi
Faculty of Animal Science, Bogor Agricultural University, Bogor 16680 Indonesia *e-mail:dewiapriastuti@yahoo.com
Abstract
Blood metabolite statues represent blood nutrients which will be metabolized by the cell. Low nutrient statues of local sheep fed with sole grass caused low performance. The aim of this experiment was to evaluate blood metabolite statues of growing male local sheep through improvement of ration containing Indigofera sp. Eight local sheep consisted of four UP3-jonggol and four garut growing sheep, were used in this experiment for three months. The rations were pellet concentrate containing 30% of Indigofera sp with 15% crude protein (CP) and 73.80% total digestible nutrient (TDN). The animals were reared in individual cages and given water and feed ad libitum. At the end of the experiment, blood was drained and collected from jugular vein using 5 ml spoit. The blood were analysed for the concentrations of plasma glucose, urea and total cholesterol using diagnostic KIT Merck Dya-Cys following laboratory standard procedure. The design of this experiment using simple T-test, the mean data was compared for all parameters. Result showed that plasma glucose concentration was not significance (77.95 vs 85.89 mg%) among the local sheep, while concentrations of blood urea-N (BUN) and plasma cholesterol in jonggol sheep (73.31 mg% and 77.18 mg%) were significant higher than in garut sheep (55.99 mg% and 58.97 mg%). It was concluded that jonggol sheep have higher plasma cholesterol and BUN compared to the garut sheep which fed by 30% Indigofera sp. in the ration.
Keywords: cholesterol, garut and jonggol sheep, glucose, Indigofera sp., urea-N
Introducton
421
Proceeding of the 2nd International Seminar on Animal Industry | Jakarta, 5-6 July 2012
meat qualty. Low nutrent status of local sheep fed wth sole grass caused low blood metabolte status and ther performance Indigofera sp. s one of legume whch has potental to be used and has 27% proten and 15.5% fber content, meanwhle the productvty s around 4,096 kg dry matter (DM)/ha (Abdullah, 2010). Fatty acd composton of Indigofera sp. s low n poly-saturated fatty acdspoly-saturated fatty acds, so t s promsng t s promsng to be used as the best part n the raton for producng good lamb meat. SubsttutonSubsttuton poly-saturated fatty acd wth poly-unsaturated fatty acd could reduce total cho-lesterol concentraton, ncludng the concentraton of LDL-chocho-lesterol (Marsc and Yodce, 1992). On the other hand, local sheep reared under the tropcal ranforest On the other hand, local sheep reared under the tropcal ranforest wth sole mxed-grass, had low blood profl status and low performance (Astut
et al., 2009). So far, t was reported that the concentratons of serum trglcerde, glucose and total proten of those sheep had only 18,71 mg% , 51,86 mg % and 6,40 mg%, respectvely. Meanwhle Wryawan et al. (2010) reported that jonggol sheep had low performance (40 g/h/d) caused by unbalance nutrtonal status and hgh daly temperature and humdty. There s no study n utlsaton of Indigofera
sp. for local sheep (garut and -jonggol) to mprove poor feedng management; so t s expected that feedng the legume wll mprove metabolte profle and lamb meat qualty.
The am of ths experment was to evaluate blood metabolte statues of grow-ng male local sheep (garut and jogrow-nggol) fed wth pellet raton contangrow-ng 30%(garut and jonggol) fed wth pellet raton contanng 30% In-digofera sp.
Materals and Methods
Eght local sheep conssted of four jonggol and four garut growng sheep
(average body weght, 20 kg), were used n ths experment for three months. The
ratons were pelleted contanng 30% of Indigofera sp. and 70% of concentrate wth total raton contaned 18% crude proten, 15.40% fber, 3.14% Extract Ether, 42.43% starch and 73.80% total dgestble nutrent (TDN). The anmals were reared n ndvdual cages and gven water and feed ad libitum. At the end of the experment, blood was draned and collected from jugular ven usng 5 ml syrnges. Whole blood was separated by centrfugaton the blood at 10.000 x g for 10 mnutes to obtan the serum. The serums were analyzed for the concentratons of glucose, blood urea ntrogen (BUN) and cholesterol usng assay kt DyaSys catalogue number 101592 as standard procedure wth spectrophotometer. The data were compared usng T-test between two local breeds and presented as fgures.
Results and Dscusson
422 Proceeding of the 2nd International Seminar on Animal Industry | Jakarta, 5-6 July 2012
raton contanng Indigofera sp. Results showed that serum glucose concentraton was not sgnfcance among two local breeds (jonggol: 77.95 mg% and garut: 85.89 mg%), whle concentratons of serum urea-N and cholesterol n jonggol sheep (73.31 mg% and 77.18 mg%) were sgnfcantly hgher than n garut sheep (55.99 mg% and 58.97 mg%) (P<0.05).
The glucose concentraton of both local sheep fed wth Indigofera sp. showed n good condton compared to the healthy sheep. Rs (1983) reported that the normal glucose concentraton of healthy sheep was 35–60 mg%. These ranges covered for35–60 mg%. These ranges covered for growng sheep (58 mg %), pregnant sheep (47 mg%) and lactatng sheep (59 mg%). The composton of Indigofera sp. raton wth hgh starch (42%) value and rapdly metabolzed may ncrease glucose concentraton n both breeds. In rumnant blood metabolte profle, glucose concentraton s low, but t s very mportant to support nutrent for the bran, nervous systems and actve organs such mammary gland (Rs,1983).
Blood urea ntrogen (BUN) s representng metabolte exes of proten. Jonggol sheep whch are usually reared n grazng pasture wth low qualty of forage has better response to the hgh qualty raton compared to garut sheep (kept n sem ntensve farmng system). The condton caused a compensatory metabolsm whch can mprove the blood metabolte status n a short tme. The evaluaton durng three months observaton showed that the concentraton of BUN n UP3-jonggol growng sheep were hgher than n garut growng sheep.
Graph 1. Glucose profle of UP3-jonggol and garut growng sheep
Table 1. Metabolte profle of local sheep fed wth Indigofera sp.
Parameters Jonggol sheep Garut sheep
Glucose (mg%) 77.95±24.73 85.89±17.87 Blood Urea (mg%) 73.31±4.18a 55.49±7.46b Cholesterol (mg%) 77.17±15.27a 58.97±9.74b
423
Proceeding of the 2nd International Seminar on Animal Industry | Jakarta, 5-6 July 2012
Graph 2. Urea-N profle of UP3-jonggol and garut growng sheep
Graph 3. Cholesterol profle of UP3-jonggol and garut growng sheep
Cholesterol status of UP3-jonggol growng sheep were hgher than garut growng sheep n the same age fed wth Indigofera sp. and both hgher than other breeds. Cox-Ganser et al. (1994) reported that blood compostons n sheep grazng Brasscas had average value of 68 mg/dl, 20 mg/dl and 64 mg/dl for glucose, BUN and cholesterol, respectvely. Some factors affectng to the cholesterol concentraton were feedng, age, physologcal state, health and breed. Cholesterol s needed for the sterods hormone precursor n growng anmals (Rs, 1983).
424 Proceeding of the 2nd International Seminar on Animal Industry | Jakarta, 5-6 July 2012
Conclusons
Analyss of bochemcal parameters of local sheep fed wth 30% Indigofera
sp. to the blood metabolte profle showed that ther values are n normal physolog-cal status. Jonggol growng sheep has hgher blood urea-N and cholesterol serum concentratons compared to garut growng sheep.
Acknowledgement
Ths research was funded by Research Grant of Peneltan Unggulan Fakultas from IPB 2011.
References
Abdullah, L. 2010. Herbage producton and qualty of shrub Indgofera treated by dfferent concentraton of folar fertlzer. Med. Pet. 33 : 169-175Pet. 33 : 169-175
Astut, D.A., R. Ekastut, Marwah and Suryan. 2009. Status Nutren dan Gambaran Darah Domba Lokal yang Dpelhara d Hutan Penddkan Gunung Walat, Sukabum. Jurnal Pertanan UNSYAH 1 : 1-8
Astut D.A. and A. Sudarman. 2009. Blood Profle and Body Composton of Sheep Fed Wth Lemuru Ol Coated By Herbs. Proc. ISAI, November, 21st
2009. Bogor Indonesa
.Cox-Ganser, J.M., G.A. Jung, R.T. Pushkn and R.L. Red. 1994. Evaluaton of Brasscas n grazng systems for sheep : II. Blood composton and nutrent status. J Anm. Sc. 72: 1832-1841
Marsc, V. and R. Yodce. 1992. The Detary Role of Monounsaturates. INFORM, 3 : 681.
Mresan, V. 2003. Evoluton of The Man Blood Indces n Tsga Fattenng Sheep. J. of Central European Agrculture 4 (4). : 405-410 (on lne).
Rs, P.M. 1983. Dynamc Bochemstry of Anmal Producton. Mc Graw Hll New York USA. pp 363.
Sudarman, A., K. G. Wryawan, H. Nuraen, M. Muttakn, dan H. Markhamah. 2006. Pengaruh pemberan sabun kalsum dar mnyak kan lemuru terhadap knerja dan kualtas dagng domba jantan lokal. Semnar Nutrs dan Teknolog Pakan. Departemen Ilmu Nutrs dan Teknolog Pakan, Fakultas Peternakan, Insttut Pertanan Bogor.
