http://jac.oxfordjournals.org/cgi/content/abstract/57/6/1231
In vitro anticandidal activity of xanthorrhizol isolated from
Curcuma xanthorrhiza Roxb
Yaya Rukayadi1,2, Dongeun Yong3 and Jae-Kwan Hwang1,*
1
Department of Biotechnology & Bioproducts Research Center (BRC), Yonsei University 134 Sinchon-dong, Seodaemun-gu, Seoul 120-749, Korea 2 Research Center for Bioresources and
Biotechnology, Bogor Agricultural University Bogor 16880, Indonesia 3 Department of Laboratory Medicine & Research Institute of Bacterial Resistance, Yonsei University College of
Medicine, Yonsei University Seoul 120-749, Korea
Received 30 July 2005;returned 19 September 2005;revised 15 March 2006;accepted 20 March 2006
*
Corresponding author. Tel: +82-2-2123-5881; Fax: +82-2-362-7265; E-mail: [email protected]
Abstract
Objectives: Xanthorrhizol, isolated from the methanol extractof Curcuma xanthorrhiza Roxb., was investigated for its anticandidalactivity using six Candida species.
Methods: The in vitro susceptibility tests for xanthorrhizolwere carried out in terms of MIC and
minimal fungicidal concentration(MFC) using the NCCLS M27-A2 broth microdilution method. Time–killcurves were determined to assess the correlation between MICand fungicidal activity of xanthorrhizol at concentrations rangingfrom 0 MIC to 4x MIC.
Results: All Candida species showed susceptibility to xanthorrhizolin the MIC range 1.0–15.0 mg/L for Candida albicans, 1.0–10 mg/L for Candida glabrata, 2.0–8.0 mg/L for Candida guilliermondii,2.5–7.5 mg/L for Candida krusei, 2.5–25 mg/L for Candida parapsilosis and
2.0–8.0 mg/L forCandida tropicalis.Time–kill curves demonstrated that xanthorrhizol was able to kill the Candida strains with MFCs of 20 mg/mL, 15 mg/mL, 12.5 mg/mL, 10 mg/L, 30 mg/mL and 10 mg/L for C. albicans, C.glabrata, C. guilliermondii, C. krusei, C. parapsilosis
andC. tropicalis, respectively.
Conclusions: The potent anticandidal activity of xanthorrhizol may support the use of C. xanthorriza for the treatment of candidiasis.
