DAFTAR PUSTAKA
Anderson JJ, Ambrose WW, Garner SC. 1995. Orally Dosed Genestein from Soy
and Prevention of Concellous Bone Loss in Two Ovariektomized Rat
Models. J. Nutr. 25: 799S (Abstract).
Anonim. 2004. Soy isoflavone aglycones have estrogenic effects on mouse
reproductive
e
tracts.http://www.obgyn.net/newsheadlines/womens_health
C
Complementary_and_Alternative_Medicine-20040304-47.asp.[31 Maret
2008].
Astuti S. 1999. Pengaruh Tepung Kedelai dan Tempe Dalam Ransum Terhadap
Fertilitas Tikus Percobaan [Tesis]. Bogor: Program Pascasarjana, Institut
Pertanian Bogor.
Arjmandi BH, Alekel L, Hollis BW, Amin D, Stacewics-Sapuntzakis M, Guo P,
Kukreja SC. 1996. Diatery Soybean Protein Prevents Bone Loss in
Ovariectomized Rat Model of Osteoporosis. J. Nutr. 126: 161-167.
Beard APM, Hunter G, Laming GE. 1994. Quantitative control of
oxytosin-induced estradiol PGF2α release by progesterone and estradiol in ewes. J
Reprod And fert. 100 : 143-150.
Cahyadi W. 2007. Kedelai Khasiat dan Teknologi. Jakarta: Bumi Aksara
Casanova M. 1999. Developmental effect of dietary phytoestrogens in Sprague-
Dawley rats and interactions of genistein and daidzein with rat estrogen
receptors alpha and beta in vitro. Toxicol Sci 51:2.
http://www.soyonlineservice.co.nz/ Abstract.html [1 Mei 2007].
Coward L, Barnes NC, Setchell KDR, Barnes S. 1993. Genestein and Daidzein
and Their β-Glucoside Conjugates: Antitumor Isoflavone in Soybean
Foods from Amerika and Asia Diets. J Agric Food Chem. 41 : 1961 –
1976.
[Deptan RI] Departemen Pertanian Republik Indonesia. 2005. Peningkatan
Produksi Kedelai (Glycine max).
Jakarta: Departemen Pertanian Republik
Indonesia Direktorat Jenderal Bina Produksi Tanaman
Pangan
D
Direktorat
K
Kacang-kacangan
d
dan
u
Umbi-umbian.
http://www.w3.org/TR/html4/frameset.dtd [1 Mei 2007].
Gadjahnata KHO. 1989. Biologi Kedokteran I. Bogor: IPB Press
Garvita RV. 2005. Efektivitas Ekstrak Kedelai pada Pra-Kebuntingan (5, 10, 15
Hari) Tikus Untuk Meningkatkan Profil Reproduksi [Tesis]. Bogor:
Program Pascasarjana, Institut Pertanian Bogor.
Guyton AC. 1994. Fisiologi Kedokteran bagian III. Ed ke-7. Jakarta: Penerbit
Buku Kedokteran EGC.
Guyton AC .1996. Fisiologi Manusia dan Mekanisme Penyakit (Human
Physiology and Mechanism of disease). Terjemahan. Ed ke-3. Jakarta:
Buku Kedokteran EGC.
Granner DK. 1990. Hormon Kelamin. Dalam: Biokimia (Harper’s Review of
Biochemestry). Diterjemahkan oleh Darmawan I. Jakarta: EGC.
Hafez ESE, Jainudeen MR, Rosnina Y. 2000. Hormones, Growth Factors and
Reproduction. Di dalam: Reproduction in Farm Animals. Ed-3.Lippincott
Williams & Wilkins. Philadelphia.hlm 31-54.
Hunter RHF. 1995. Fisiologi dan Teknologi Reproduksi Hewan Betina Domestik.
Alih bahasa D.K. Harya Putra. Bandung : Institut Teknologi Bandung
Press.
Hardjopranjoto S. 1995. Ilmu Kemajiran pada Ternak. Surabaya. Airlangga
University Press.
Heinnermen J. 2003. Peran isoflavon untuk kesehatan reproduksi wanita. Cermin
Dunia Kedokteran 139.
[IHME] Indonesian Hospital Medical Equipment. 2007. Isoflavon, Makanan
Ajaib.Jakarta:
PP
Pusat
D
Data
aa
&
I
Informasi
P
PERSI.
h
http://www.pdpersi.c
o.id/images/news/content/tempe.jpg [11 April 2008].
Jeferson. 2002. Assessing estrogenic activity of phytochemicals using
transcriptional activation and immature mouse uterotropic responses. J
chromatogr B Analyt Technol
B
Biomed
L
Life
S
Sci
7777:179.
Jones MEE, Thorburn AW, Britt KI, Hewitt KN, Wreford NG, Proietto J, Oz OK,
Leury BJ, Robertson KM, Yao S, Simpson ER. 2000. Aromatase-Deficient
(ArKO) Mice have Phenotype of Increased Adiposity. Proc. Natl. Acad.
Sci. USA. 97(23): 12735-12740.
Koswara S. 1992. Teknologi Pengolahan Kedelai Menjadikan Makanan Bermutu.
Jakarta: Pustaka Sinar Harapan.
Koswara S. 1995. Teknologi Pengolahan Kedelai Menjadikan Makanan Bermutu.
Jakarta: Pustaka Sinar Harapan.
Kuiper GGJM, Carlsson B, Grandien K, Enmark E, Haggblod J, Nilsson S,
Gustafsson JA. 1997. Comparison of the Ligand Binding Specificity and
Transcript Tissue Distribution of Estrogen Receptor α and β. J.
Malole MBM, Pramono CSU. 1989. Penggunaan Hewan-hewan Percobaan di
Laboratorium. Departemen Pendidikan dan Kebudayaan, Dirjen
Pendidikan Tinggi, Pusat Antar Universitas Bioteknologi. Bogor: IPB
Press.
Mattjik AA, Sumertajaya IM. 2006. Perancangan Percobaan Dengan Aplikasi
SAS dan MINITAB. Ed ke-3. Bogor: IPB Press.
Murata K, Ikehata H, Miyamoto T. 1976. Studies on the Ntritional Value of
Tempeh. J Food Sci. 32(5):580-586.
Nakajima N, Nozak N, Ishihara N, Ishikawa A, Tsuji H. 2005. Analysis of
Isoflavone Content in Tempeh, a Fermented Soybean, and Preparation of a
New Isoflavone- Enriched Tempeh. Journal of Bioscience and
Bioengineering vol.100.No.6, 685-6876.2005.DOL:10.1263/jbb.100.685.
Nalbandov AV. 1990. Fisiologi Reproduksi pada Mamalia dan Unggas. Alih
bahasa S. Keman. Jakarta: Universitas Indonesia Press.
Nurdin SU. 2002. Pengaruh Pemberian Tepung Tahu dan Tempe Kedelai dalam
Ransum terhadap Massa dan Densitas Tulang Tikus Betina
Ovariektomi.[Thesis].Bogor: Program Pasca Sarjana.
Pakasi LS. 2000. Monopouse: Masalah dan Penanggulangannya. Jakarta:
Fakultas Kedokteran Universitas Indonesia.
Pawiroharsono
S
S.
2
2007.
Prospek dan Manfaat Isoflavon untuk Kesehatan.
Jakarta: Direktorat Teknologi Bioindustri, Badan Pengkajian dan
Penerapan
T
Teknologi.
h
http://www.tempointeraktif.com/medika/arsip/042
001/indexisi.asp?fi:epus
[1 Mei 2007]
Persky VW, Turyk ME,Lingt W,Freels S,Barnes S.2002. Effect of soy protein on
endogenous in postmenopausal women. Am J. Clin Nutr.75:145-153.
Pineda MH. 1989. Female reproductive system. London: Lea & Febiger.
Qiju W. 2003. Purification and antioxidant activities of soybean isoflavon. A
Thesis Submitted to the Graduate Faculty of the Louisiana State
University and Agricultural and Mechanical College in partil filfillment of
the requirements for the degree of Master of Science in The Department of
Food Science B.S.,Zhejiang University.
Santell RC, Chang YC, Nair MG, Helferich WG. 1997. Dietary Genestein Exerts
Estrogenic Effects Upon the Uterus, Mammary Gland, and the
Hyphothalamic/Pituitary Axis in Rats. J. Nutr. 127: 263-269.
Setchell KDR, Cassidy A. 1999. Dietary isoflavon: Biological effect and
relevance to human health. Symposium on Phytoestrogenicals:
Biochemistry and Physiology. J Nutr.129:758S-767S.
Shurtleff WA, Aoyagi. 1979. The Book of Tempeh. New York: Harper and Row.
Smith JB, Mangkoewidjojo S. 1988. Pemeliharaan, pembiakan dan penggunaan
hewan percobaan di daerah tropis. Jakarta: U Press.
Tanu I. 2005. Farmakologi dan Terapi. Ed-4. Jakarta: Bagian Farmakologi
Fakultas Kedokteran Universitas Indonesia.
Toelihere MR. 1985. Fisiologi reproduksi pada ternak. Bandung: Angkasa.
Turner CD, Bagnara JT. 1976. Endokrinologi Umum. Ed ke-6. Yogyakarta: Air
Langga University Press.
Whitten PL, Patisaul HB. 2001. Cross-species and interassay comparisons of
phytoestrogenaction.
E
Environ
H
Health
P
Perspect.
h
http://www.soyonlinese
rvice.co.nz/htm [1 Mei 2007].
[Wikipedia]
W
Wikipedia.
2
2007.
B
Brown
R
Rat.
h
http://en.wikipedia.org/wiki/Imag
e:Rattus norvegicus_1.jpg [1 Mei 2007].
[Wikipedia]
W
Wikipedia.
2
2007.
S
Soybean.http://en.wikipedia.org/wiki/Image:Soy
bean.USDA.jpg [1 Mei 2007]
Winarsi H. 2005. Isoflavon berbagai sumber, sifat, dan manfatnya pada penyakit
degeneratif. Yogyakarta: Gadjah mada University Press.
Wodzicka-Tomaszewska M, Sutama IK, Putu IG, Chaniago. 1991. Reproduksi,
Tingkah Laku dan Produksi Ternak di Indonesia. Jakarta: Gramedia.
Wuryani W. 1995. Isoflavone in Tempe. Asean Food Journal. 3(10): 99-102.
Yulianto.
2
2003.
K
Kedelai,
B
Bahan
P
Pangan
P
Penyayang
T
Tulang.
h
http://www.
sinarhar pan.coid/iptek/kesehatan/2003/1024/kes1.html.[16 Mei 2007]
Zhang Y, Song TT, Cunnick JE, Murphy PA, Hendric HS. 1999. Daidzein and
genistein glucuronides in vitro are weakly estrogenic and active human
natural killer cell at nutritionally relevant concentrations. J.
Nutr.129:399-405.
Lampiran 1. Proses pembuatan tempe
KEDELAI
Pembersihan
TEMPE
Pencucian + Perendaman
Pendinginan (suhu kamar)
Perebusan (100
0C, 30 Menit)
Pembungkusan dengan plastik
Peragian (2 gr/kg kedelai)
Perendaman dengan air rebusan (22 Jam)
Pengupasan kulit
Perebusan dalam air asam (45 menit)
Lampiran 2. Proses pembuatan tepung kedelai
Kedelai
Penggilingan (diblender)
Pengeringan (oven), suhu 45
0C, kadar air 10%
Penepungan (disk mill)
Pengayakan (60 mesh)
Lampiran 3. Proses pembuatan tepung tempe
Tempe Mentah
Penggilingan (diblender)
Penepungan (disk mill)
Pengayakan (60 mesh)
Tepung Tempe
Pengirisan Tempe (1 x 2 x 0,5 cm
3)
Lampiran 4. Prosedur analisis senyawa isoflavon.
Ekstraksi Tepung Kedelai dan Tepung Tempe
Tepung kedelai dan tepung tempe dikeringkan selama 4 jam. Selanjutnya
diekstrak dengan larutan methanol 99,8% sebanyak 2 kali, masing-masing 100 ml
dengan menggunakan pompa vakum yang sudah dilapisi kertas kering, sehingga
metanol yang digunakan untuk mengekstrak sebanyak 200 ml. Senyawa isoflavon
termasuk senyawa polar sehingga mudah larut dengan larutan polar seperti
alkohol atau metanol. Ekstraksi dilakukan 2 kali agar senyawa isoflavon dalam
tepung kedelai dan tepung tempe terekstrak secara keseluruhan.
Ekstrak yang diperoleh disimpan dalam ruang dingin kurang lebih 0
0C
selama satu malam untuk menggumpalkan lemaknya. Lemak yang ada dipisahkan
melalui penyaringan dengan pompa vakum yang dilapisi kertas saring. Filtrat
yang diperoleh selanjutnya diuapkan dengan rotavapor pada suhu 40
0C. Ekstrak
yang telah kering dilarutkan dalam 10 ml metanol absolute (p.a), kemudian
disentrifuse pada kecepatan 4000 rpm selama 5 menit dengan tujuan untuk
mendapatkan filtrate yang bening dan memisahkan endapan yang terbentuk.
Pemurnian Isoflavon
Filtrat yang terbentuk diambil 1.5 ml dan dimurnikan dalam kolom
kromatografi volume 12 ml yang berisi MN-Poliamida CC6 dengan ukuran
partikel 0.05-0.16 mm. Sebelum filtrate dituangkan ke dalam kolom,
MN-Poliamida CC6 direndam semalam dalam kromatografi dengan larutan metanol
25%. Elusi dalam kolom dilakukan secara bertahap dengan metanol 25%, 50%,
70%, masing-masing 50 ml. Eluen yang didapat dari elusi metanol 70% (fraksi
70%) ditampung dan dikeringkan dengan rotavapor pada suhu 40
0C sampai
kering. Residu (endapan kering yang diperoleh) dilarutkan dalam 1 ml metanol
absolut dan disentrifuse pada 4000 rpm selama 5 menit untuk memisahkan
endapan yang ada, kemudian disaring. Filtrat yang bening siap untuk dianalisa
dengan menggunakan HPLC.
Identifikasi Isoflavon
Analisis
kuantitatif
dilakukan dengan kromatografi cairan tekanan tinggi
(HPLC). HPLC yang digunakan berada pada kondisi sebagai berikut:
Volume sampel
: 40 µl
Kolom
: Li chrosorb RP-18 (250 x 4 mm, 5 µm)
Eluen
: Asam asetat 3% (pelarut A); Acetonitril (pelarut B)
Gradien
: 20% dalam (A + B) sampai 60% (A+B) selama 35 menit.
Detektor
: UV 261 nm
Kecepatan alir
: 0.8 ml/menit
50 g Sampel dikeringkan pada suhu 40
0c selama 5 jam
Diektrasksi dengan metanol absolut dua kali masing-masing 100 ml, disaring
dengan dengan pompa vakum
Ekstrak disimpan dalam ruang pendingin 0
0C selama semalam untuk
menggumpalkan lemaknya
Lemak dipisahkan dengan pompa vakum
Filtrat jernih diuapkan dengan rotavapor pada suhu 40
0C
Residu dilarutkan dalam 10 ml metanol absolut
Disentrifuse pada kecepatan 4000 rpm selama 5 menit
Diambil 1.5 ml filtrat untuk dipurifukasi dalam kolom kromatografi yang berisi
MN-Poliamida CC6
Dielusi bertahap dengan metanol 25%, 50% dan 70%, masing-masing 50 ml
Hasil elusi dengan 70% metanol dikeringkan dengan rotavapor pada suhu 40
0C
Residu dilarutkan dalam ml metanol grade HPLC
Disentrifusi pada 4000 rpm selama 5 menit
Diambil 40 µl untuk diinjeksi pada HPLC
Lampiran 5. Contoh perhitungan kandungan senyawa isoflavon.
NP 289 (Tepung Kedelai).
206.37 ppm
NP 290 (Tepung Tempe).
ANALISIS STATISTIK PERSENTASE PERUBAHAN BERAT BADAN TIKUS MINGGU I hasil penelitian
The ANOVA Procedure Class Level Information Class Levels Values
perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 The ANOVA Procedure
Dependent Variable: persentase perubahan berat badan minggu I (PPBB MI) Sum of
Source DF Squares Mean Square F Value Pr > F Model 3 0.18578000 0.06192667 0.75 0.5408 Error 16 1.32980000 0.08311250
Corrected Total 19 1.51558000
R-Square Coeff Var Root MSE PPBB MI Mean 0.122580 11.86877 0.288292 2.429000 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 0.18578000 0.06192667 0.75 0.5408 The ANOVA Procedure
Duncan's Multiple Range Test for PPBB MI
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 0.083113
Number of Means 2 3 4 Critical Range .3865 .4053 .4171 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan
A 2.5860 5 Non OV A A 2.4280 5 OV Tp A A 2.3640 5 Kontrol A A 2.3380 5 OV Kd
ANALISIS STATISTIK PERSENTASE PERUBAHAN BERAT BADAN TIKUS MINGGU II hasil penelitian
The ANOVA Procedure Class Level Information Class Levels Values
perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 The ANOVA Procedure
Dependent Variable: persentase perubahan berat badan minggu II (PPBB MII) Sum of
Source DF Squares Mean Square F Value Pr > F Model 3 111.0838150 37.0279383 573.65 <.0001 Error 16 1.0327600 0.0645475
Corrected Total 19 112.1165750
R-Square Coeff Var Root MSE PPBB MII Mean 0.990789 16.74214 0.254062 1.517500 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 111.0838150 37.0279383 573.65 <.0001 The ANOVA Procedure
Duncan's Multiple Range Test for PPBB MII
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 0.064547
Number of Means 2 3 4 Critical Range .3406 .3572 .3676 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan
A 3.5000 5 Non OV A
A 3.4080 5 OV Kd B 1.4800 5 OV Tp C -2.3180 5 Kontrol
ANALISIS STATISTIK PERSENTASE PERUBAHAN BERAT BADAN TIKUS MINGGU III hasil penelitian
The ANOVA Procedure Class Level Information Class Levels Values
perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 The ANOVA Procedure
Dependent Variable: persentase perubahan berat badan minggu III (PPBB MIII) Sum of
Source DF Squares Mean Square F Value Pr > F Model 3 144.2472150 48.0824050 478.32 <.0001 Error 16 1.6083600 0.1005225
Corrected Total 19 145.8555750
R-Square Coeff Var Root MSE PPBB MIII Mean 0.988973 13.68081 0.317053 2.317500 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 144.2472150 48.0824050 478.32 <.0001 The ANOVA Procedure
Duncan's Multiple Range Test for PPBB MIII
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 0.100522
Number of Means 2 3 4 Critical Range .4251 .4458 .4587 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan
A 4.5720 5 Non OV B 3.4900 5 OV Tp B
B 3.4780 5 Kontrol C -2.2700 5 OV Kd
ANALISIS STATISTIK PERSENTASE PERUBAHAN BERAT BADAN TIKUS MINGGU IV hasil penelitian
The ANOVA Procedure Class Level Information Class Levels Values
perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20
Number of Observations Used 20 The ANOVA Procedure
Dependent Variable: persentase perubahan berat badan minggu IV (PPBB MIV) Sum of
Source DF Squares Mean Square F Value Pr > F Model 3 4.05452000 1.35150667 4.86 0.0137 Error 16 4.44916000 0.27807250
Corrected Total 19 8.50368000
R-Square Coeff Var Root MSE PPBB MIV Mean 0.476796 9.373015 0.527326 5.626000 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 4.05452000 1.35150667 4.86 0.0137 The ANOVA Procedure
Duncan's Multiple Range Test for PPBB MIV
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 0.278073
Number of Means 2 3 4 Critical Range .7070 .7414 .7629 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan
A 6.0860 5 Non OV A A 6.0480 5 OV Tp B 5.3120 5 OV Kd B B 5.0580 5 Kontrol
ANALISIS STATISTIK PERSENTASE KENAIKAN BERAT BADAN TIKUS hasil penelitian
The ANOVA Procedure Class Level Information Class Levels Values
perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 The ANOVA Procedure
Dependent Variable: persentase kenaikan berat badan Sum of
Source DF Squares Mean Square F Value Pr > F Model 3 6075.568100 2025.189367 10.34 0.0005 Error 16 3134.753120 195.922070
Corrected Total 19 9210.321220
R-Square Coeff Var Root MSE persentase kenaikan berat badan Mean 0.659648 43.54670 13.99722 32.14300 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 6075.568100 2025.189367 10.34 0.0005 The ANOVA Procedure
Duncan's Multiple Range Test for persentase kenaikan berat badan
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 195.9221
Number of Means 2 3 4 Critical Range 18.77 19.68 20.25 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan
A 56.112 5 Non OV B 35.244 5 OV Tp B
B 30.110 5 Kontrol C 7.106 5 OV Kd
ANALISIS STATISTIK BERAT BADAN AKHIR TIKUS hasil penelitian
The ANOVA Procedure Class Level Information Class Levels Values perlakuan 4 P1 P2 P3 P4 Number of Observations Read 20 Number of Observations Used 20 The ANOVA Procedure
Dependent Variable: berat badan akhir
Sum of
Source DF Squares Mean Square F Value Pr > F Model 3 2961.600000 987.200000 7.74 0.0020 Error 16 2040.400000 127.525000
Corrected Total 19 5002.000000
R-Square Coeff Var Root MSE berat badan akhir Mean 0.592083 4.277537 11.29270 264.0000 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 2961.600000 987.200000 7.74 0.0020 The ANOVA Procedure
Duncan's Multiple Range Test for berat badan akhir
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 127.525
Number of Means 2 3 4 Critical Range 15.14 15.88 16.34 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan
A 275.600 5 P1 A A 275.600 5 P4 B 257.600 5 P3 B B 247.200 5 P2
ANALISIS STATISTIK BERAT UTERUS TIKUS Hasil Penelitian
The ANOVA Procedure Class Level Information Class Levels Values
perlakuan 4 Non OV Kontrol OV Kd Ov Tp Number of Observations Read 20
Number of Observations Used 20 The ANOVA Procedure
Dependent Variable: berat uterus
Sum of
Source DF Squares Mean Square F Value Pr > F Model 3 0.35765500 0.11921833 184.83 <.0001 Error 16 0.01032000 0.00064500
Corrected Total 19 0.36797500
R-Square Coeff Var Root MSE berat uterus Mean 0.971955 7.638150 0.025397 0.332500 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 0.35765500 0.11921833 184.83 <.0001 The ANOVA Procedure
Duncan's Multiple Range Test for berat uterus NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 0.000645
Number of Means 2 3 4 Critical Range .03405 .03571 .03674 Means with the same letter are not significantly different. Duncan Grouping Mean N Perlakuan
A 0.46600 5 Non OV A
A 0.45800 5 OV Tp B 0.25000 5 OV Kd C 0.15600 5 Kontrol
ANALISIS STATISTIK BERAT UTERUS PER BERAT BADAN TIKUS hasil penelitian
The ANOVA Procedure Class Level Information Class Levels Values
perlakuan 4 Kontrol Kontrol OV Kd OV Tp Number of Observations Read 20
Number of Observations Used 20 The ANOVA Procedure
Dependent Variable: berat uterus/berat badan tikus Sum of
Source DF Squares Mean Square F Value Pr > F Model 3 0.04041500 0.01347167 145.64 <.0001 Error 16 0.00148000 0.00009250
Corrected Total 19 0.04189500
R-Square Coeff Var Root MSE berat uterus/berat badan tikus Mean 0.964674 8.048278 0.009618 0.119500 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 0.04041500 0.01347167 145.64 <.0001 The ANOVA Procedure
Duncan's Multiple Range Test for berat uterus/berat badan tikus
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 0.000092
Number of Means 2 3 4 Critical Range .01289 .01352 .01391 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan
A 0.166000 5 Non OV A
A 0.165000 5 OV Tp B 0.092000 5 OV Kd C 0.060000 5 Kontrol
UJI KORELASI DAN REGRESI PERSENTASE KENAIKAN BERAT BADAN TERHADAP RASIO BERAT UTERUS PER BERAT BADAN TIKUS
The SAS System The CORR Procedure 2 Variables: x y Simple Statistics
Variable N Mean Std Dev Sum Minimum Maximum x 4 0.12250 0.05620 0.49000 0.06000 0.17000 y 4 32.14000 20.12323 128.56000 7.11000 56.11000
Pearson Correlation Coefficients, N = 4 Prob > |r| under H0: Rho=0 x y x 1.00000 0.65635 0.3437 y 0.65635 1.00000 0.3437 The GLM Procedure
Number of Observations Read 4 Number of Observations Used 4 The GLM Procedure
Dependent Variable: y
Sum of
Source DF Squares Mean Square F Value Pr > F Model 1 523.339128 523.339128 1.51 0.3437 Error 2 691.494272 345.747136
Corrected Total 3 1214.833400
R-Square Coeff Var Root MSE y Mean 0.430791 57.85400 18.59428 32.14000 Source DF Type I SS Mean Square F Value Pr > F x 1 523.3391282 523.3391282 1.51 0.3437 Source DF Type III SS Mean Square F Value Pr > F x 1 523.3391282 523.3391282 1.51 0.3437 Standard
Parameter Estimate Error t Value Pr > |t| Intercept 3.3502375 25.1797894 0.13 0.9063 x 235.0184697 191.0247704 1.23 0.3437
ANALISIS STATISTIK PANJANG UTERUS KIRI TIKUS Hasil Penelitian
The ANOVA Procedure Class Level Information Class Levels Values
perlakuan 4 Non oV Kontrol OV Kd OV Tp Number of Observations Read 20
Number of Observations Used 20 The ANOVA Procedure
Dependent Variable: panjang uterus kiri
Sum of
Source DF Squares Mean Square F Value Pr > F Model 3 2.35750000 0.78583333 16.81 <.0001 Error 16 0.74800000 0.04675000
Corrected Total 19 3.10550000
R-Square Coeff Var Root MSE panjang uterus kiri Mean 0.759137 8.113226 0.216217 2.665000 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 2.35750000 0.78583333 16.81 <.0001
The ANOVA Procedure
Duncan's Multiple Range Test for panjang uterus kiri NOTE: This test controls the Type I comparisonwise error rate, not the
experimentwise error rate.
Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 0.04675
Number of Means 2 3 4 Critical Range .2899 .3040 .3128 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan
A 3.2000 5 Non OV B 2.6800 5 OV Tp B C B 2.5200 5 OV Kd C C 2.2600 5 Kontrol
ANALISIS STATISTIK PANJANG UTERUS KANAN TIKUS Hasil Penelitian
The ANOVA Procedure Class Level Information Class Levels Values
perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20
Number of Observations Used 20
The ANOVA Procedure Dependent Variable: panjang uterus kanan
Sum of
Source DF Squares Mean Square F Value Pr > F Model 3 3.24950000 1.08316667 50.97 <.0001 Error 16 0.34000000 0.02125000
Corrected Total 19 3.58950000
R-Square Coeff Var Root MSE panjang uterus kanan Mean 0.905279 6.086589 0.145774 2.395000 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 3.24950000 1.08316667 50.97 <.0001
The ANOVA Procedure
Duncan's Multiple Range Test for panjang uterus kanan NOTE: This test controls the Type I comparisonwise error rate, not the
experimentwise error rate.
Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 0.02125
Number of Means 2 3 4 Critical Range .1954 .2050 .2109 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan
A 3.08000 5 Non OV B 2.28000 5 OV Tp B C B 2.16000 5 OV Kd C C 2.06000 5 Kontrol
ANALISIS STATISTIK TOTAL PANJANG UTERUS TIKUS hasil penelitian The ANOVA Procedure Class Level Information Class Levels Values
perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 The ANOVA Procedure
Dependent Variable: total panjang uterus
Sum of
Source DF Squares Mean Square F Value Pr > F Model 3 10.95401500 3.65133833 2938.70 <.0001 Error 16 0.01988000 0.00124250
Corrected Total 19 10.97389500
R-Square Coeff Var Root MSE totalpanjanguterus Mean 0.998188 0.696692 0.035249 5.059500 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 10.95401500 3.65133833 2938.70 <.0001 The ANOVA Procedure
Duncan's Multiple Range Test for total panjang uterus
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 0.001243
Number of Means 2 3 4 Critical Range .04726 .04956 .05100 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan
A 6.28000 5 Non Ov B 4.95800 5 OV Tp C 4.68000 5 OV Kd D 4.32000 5 Kontrol
ANALISIS STATISTIK BERAT BASAH BEBAS LEMAK UTERUS TIKUS Hasil Penelitian
The ANOVA Procedure Class Level Information Class Levels Values
perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20
Number of Observations Used 20 The ANOVA Procedure
Dependent Variable: berat basah bebas lemak uterus Sum of
Source DF Squares Mean Square F Value Pr > F Model 3 0.07625500 0.02541833 78.21 <.0001 Error 16 0.00520000 0.00032500
Corrected Total 19 0.08145500
R-Square Coeff Var Root MSE berat basah bebas lemak uterus Mean 0.936161 9.081993 0.018028 0.198500 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 0.07625500 0.02541833 78.21 <.0001 The ANOVA Procedure
Duncan's Multiple Range Test for berat basah bebas lemak uterus NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 0.000325
Number of Means 2 3 4 Critical Range .02417 .02535 .02608 Means with the same letter are not significantly different. Duncan Grouping Mean N Perlakuan
A 0.26200 5 Non Ov A
A 0.25400 5 OV Tp B 0.16200 5 OV Kd C 0.11600 5 Kontrol
ANALISIS STATISTIL BERAT KERING BEBAS LEMAK UTERUS TIKUS Hasil Penelitian
The ANOVA Procedure Class Level Information Class Levels Values
perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 The ANOVA Procedure
Dependent Variable: berat kering bebas lemak uterus Sum of
Source DF Squares Mean Square F Value Pr > F Model 3 0.00453500 0.00151167 75.58 <.0001 Error 16 0.00032000 0.00002000
Corrected Total 19 0.00485500
R-Square Coeff Var Root MSE berat kering bebas lemaku terus Mean 0.934089 12.25243 0.004472 0.036500 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 0.00453500 0.00151167 75.58 <.0001 The ANOVA Procedure
Duncan's Multiple Range Test for berat kering bebas lemaku terus
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 0.00002
Number of Means 2 3 4 Critical Range .005996 .006288 .006470 Means with the same letter are not significantly different. Duncan Grouping Mean N Perlakuan
A 0.058000 5 Non OV B 0.042000 5 OV Tp C 0.028000 5 OV Kd D 0.018000 5 Kontrol
ANALISIS STATISTIK BERAT BADAN TIKUS MINGGU I hasil penelitian
The ANOVA Procedure Class Level Information Class Levels Values
perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20
Number of Observations Used 20 The ANOVA Procedure
Dependent Variable: berat badan minggu I
Sum of
Source DF Squares Mean Square F Value Pr > F Model 3 13689.45000 4563.15000 11.37 0.0003 Error 16 6419.51200 401.21950
Corrected Total 19 20108.96200
R-Square Coeff Var Root MSE berat badan minggu I Mean 0.680764 9.530601 20.03046 210.1700 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 13689.45000 4563.15000 11.37 0.0003 The ANOVA Procedure
Duncan's Multiple Range Test for berat badan minggu I NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 401.2195
Number of Means 2 3 4 Critical Range 26.86 28.16 28.98 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan
A 241.34 5 OV Kd A A 229.20 5 OV Tp B 193.56 5 Kontrol B B 176.58 5 Non OV
ANALISIS STATISTIK BERAT BADAN TIKUS MINGGU II hasil penelitian
The ANOVA Procedure Class Level Information Class Levels Values
perlakuan 4 Nn OV Kontrol OV Kd OV Tp Number of Observations Read 20
Number of Observations Used 20 The ANOVA Procedure
Dependent Variable: berat badan minggu II
Sum of
Source DF Squares Mean Square F Value Pr > F Model 3 13108.70000 4369.56667 12.06 0.0002 Error 16 5796.99200 362.31200
Corrected Total 19 18905.69200
R-Square Coeff Var Root MSE berat badan minggu II Mean 0.693373 8.780559 19.03450 216.7800 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 13108.70000 4369.56667 12.06 0.0002 The ANOVA Procedure
Duncan's Multiple Range Test for berat badan minggu II NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 362.312
Number of Means 2 3 4 Critical Range 25.52 26.76 27.54 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan
A 257.80 5 OV Kd B 214.94 5 Non OV B C B 206.36 5 OV Tp C C 188.02 5 Kontrol
ANALISIS STATISTIK BERAT BADAN TIKUS MINGGU III
hasil penelitian
The ANOVA Procedure Class Level Information Class Levels Values
perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 The ANOVA Procedure
Dependent Variable: berat badan minggu III
Sum of
Source DF Squares Mean Square F Value Pr > F Model 3 4913.91350 1637.97117 4.45 0.0186 Error 16 5887.16400 367.94775
Corrected Total 19 10801.07750
R-Square Coeff Var Root MSE berat badan minggu III Mean 0.454947 8.330025 19.18196 230.2750 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 4913.913500 1637.971167 4.45 0.0186 The ANOVA Procedure
Duncan's Multiple Range Test for berat badan minggu III NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 367.9477
Number of Means 2 3 4 Critical Range 25.72 26.97 27.75 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan
A 245.96 5 OV Tp A A 241.10 5 Non OV A B A 228.60 5 OV Kd B B 205.44 5 OV Tp
ANALISIS STATISTIK BERAT BADAN TIKUS MINGGU III hasil penelitian
The ANOVA Procedure Class Level Information Class Levels Values
perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20
Number of Observations Used 20 The ANOVA Procedure
Dependent Variable: berat badan minggu IV
Sum of
Source DF Squares Mean Square F Value Pr > F Model 3 2961.600000 987.200000 7.74 0.0020 Error 16 2040.400000 127.525000
Corrected Total 19 5002.000000
R-Square Coeff Var Root MSE berat badan minggu IV Mean 0.592083 4.277537 11.29270 264.0000 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 2961.600000 987.200000 7.74 0.0020 The ANOVA Procedure
Duncan's Multiple Range Test for berat badan minggu IV NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 127.525
Number of Means 2 3 4 Critical Range 15.14 15.88 16.34 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan
A 275.600 5 Non OV A A 275.600 5 OV Tp B 257.600 5 OV Kd B B 247.200 5 Kontrol