View of Method Validation and Estimation of Measurement Uncertainty in the Determination of Total Polyphenols Content in Land Spinach by UV-Vis Spectrophotometry

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Method Validation and Estimation of Measurement Uncertainty in The Determination of Total Polyphenols Content in Land Spinach by Uv-Vis Spectrophotometry

≤

⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯

𝐿𝑂𝐷 = 3 𝑥_{𝑆𝑙𝑜𝑝𝑒}^{𝑆𝑦/𝑥}

𝐿𝑂𝑄 = 10 𝑥^{𝑆𝑦/𝑥}

𝑠𝑙𝑜𝑝𝑒

Sy/x = √^{Ʃ (𝑦−𝑦1)²}

𝑛−2

𝑆𝐷 = √^{Ʃ (𝑥𝑖−𝑥)²}_𝑛−1

%RSD =^𝑆𝐷_𝑥 𝑥100%

% Recovery = ^𝐴−𝐵

𝐶 𝑥100%

-0.2 0.0 0.2 0.4 0.6 0.8

0.0 0.2 0.4 0.6 0.8 1.0 1.2

Absorbance

Concentration (mg/L)

Table 3.

µx x Unit µx/x (µx/x)²