LAMPIRAN
Lampiran 1. Deskripsi Pembuatan Larutan Stok
1. Pembuatan Larutan Stok
a. Tris HCl 1M pH 7.4 (50 ml) Bahan yang digunakan adalah : - Tris : 6.057 gr
- Aquades ditambahkan hingga volume larutan mendekati 50 ml
- Pengaturan pH dilakukan dengan menambahkan NaOH 2.5 M hingaa pH 7.4 - Volume ditepatkan hingga 50 ml
- Larutan disterilisasi dengan autoclave b. EDTA 0.5 MpH 8.0 (100ml)
- NaEDTA : 18.612 gr - NaOH : 2.0 gr - Aquades : 80 ml
- Larutan dibuat dengan mencampurkan bahan kimia dengan gelas beker dan diaduk dengan batang pengaduk magnetik
- Pengaturan pH dilakukan dengan menambahkan HCl hingga pH 8.0 - Volume ditepatkan dengan aquades hingga 100 ml
- Larutan disterilkan dengan autoclave 2. Pembuatan Larutan Kerja
a. Buffer TAE 50 X (100 ml) Bahan yang digunakan adalah : - Tris : 10 ml
- Asam Asetat Glasial : 5.7 ml - EDTA 0.5 M pH 8.0 : 10 ml
- Aquades ditambahakan hingga volume larutan 100 ml b. Buffer TAE 1X (500 ml)
Bahan yang digunakan adalah : - Buffer TAE 50 X : 10 ml - Aquades : 490 ml
Lampiran 2. Alur Penelitian
Stok DNA
Lampiran 4. Data Dissimilarity Index (Bagian I)
Units 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Data Dissimilarity Index (Bagian II)
Dissimilarity calculated from data file: Klon BTC 60 (Data Biner).var (type:'single') User selection Units: 30/30 and Variables: 13/13
Dissimilarity index: Presence / Absence - Dice Missing data options:
Integer code for missing data = 999
Pairwise variable deletion - Remove variables with missing data for the current unit pair (50 % of valid data required for each unit pair)
Lampiran 5. Data Factorial Coordinates
Units Axis 1 Axis 2 Axis 3 Axis 4 Axis 5
1 0.0155135 -0.0841746 0.0520552 -0.0157423 0.0000382
2 -0.0936679 0.0071254 0.0287942 -0.0007886 0.0040785
3 0.0045261 0.0127436 -0.0194586 0.0070029 -0.0000438
4 0.0155135 -0.0841746 0.0520552 -0.0157423 0.0000382
5 -0.0526853 -0.0380017 -0.0116932 -0.0003752 -0.0002894
6 0.0635129 -0.0155398 0.0032464 0.0039986 0.0000411
7 0.0045261 0.0127436 -0.0194586 0.0070029 -0.0000438
8 0.0635129 -0.0155398 0.0032464 0.0039986 0.0000411
9 0.0045261 0.0127436 -0.0194586 0.0070029 -0.0000438
10 0.0045261 0.0127436 -0.0194586 0.0070029 -0.0000438
11 0.0635129 -0.0155398 0.0032464 0.0039986 0.0000411
12 0.0635129 -0.0155398 0.0032464 0.0039986 0.0000411
13 -0.0526853 -0.0380017 -0.0116932 -0.0003752 -0.0002894 14 -0.0526853 -0.0380017 -0.0116932 -0.0003752 -0.0002894 15 0.0045261 0.0127436 -0.0194586 0.0070029 -0.0000438 16 -0.0283521 0.1082510 0.0250490 0.0422119 -0.0000575 17 -0.0526853 -0.0380017 -0.0116932 -0.0003752 -0.0002894 18 -0.0526853 -0.0380017 -0.0116932 -0.0003752 -0.0002894 19 0.0045261 0.0127436 -0.0194586 0.0070029 -0.0000438 20 0.0130242 0.0440545 -0.0645463 -0.0434998 0.0000534 21 0.0045261 0.0127436 -0.0194586 0.0070029 -0.0000438 22 -0.0295586 0.1549966 0.0046898 -0.0279103 0.0001936 23 0.1201970 0.0759274 0.0501061 -0.0134748 -0.0000835 24 -0.0526853 -0.0380017 -0.0116932 -0.0003752 -0.0002894
25 0.0635129 -0.0155398 0.0032464 0.0039986 0.0000411
26 0.0045261 0.0127436 -0.0194586 0.0070029 -0.0000438
27 0.0635129 -0.0155398 0.0032464 0.0039986 0.0000411
28 -0.1246720 0.0508358 0.0665933 -0.0064400 -0.0021727 29 -0.0526853 -0.0380017 -0.0116932 -0.0003752 -0.0002894
30 0.0635129 -0.0155398 0.0032464 0.0039986 0.0000411
Factorial coordinates calculated from dissimilarity: Klon BTC 60 (Data Biner).dis Cosinus² values recorded in: Klon BTC 60 (Data Biner)-PCoA_Cos.DON
Axis Eigenvalue Inertia%
1 0.00296 36.11
2 0.00245 29.78
Lampiran 6. Data Output DARwin .Arb
1 33 0
2 35 0.0255184587850491
3 58 0
16 31 0.0026762523191094
17 45 0
18 44 0
19 56 0
20 51 0.043594465962887
21 52 0
22 31 0.0373237476808906
23 34 0
24 46 0
25 37 0
26 57 0
27 39 0
28 32 0.0332477273552788
29 43 0
30 40 0
Lanjutan…
31 32 0.0101580697461704 32 35 0.0329478926446892 33 42 0.0347278387032644
34 36 0
35 49 0.0351828652416321
36 37 0
37 38 0
38 39 0
39 40 0
40 41 0
41 42 0.0306316384209186 42 50 0.0307379872992196
43 44 0
44 45 0
45 46 0
46 47 0
47 48 0
48 49 0.0296623597723572 49 50 0.0176206325083798 50 51 0.0022689819853339 51 52 0.0040245816561606
52 53 0 Tree constructed from: Klon BTC 60 (Data Biner).dis 30 selected units on 30
Bootstrap analysis: 1000
Average 'edge' distance between bootstrapped trees: 0.8598 5-percentile: 0.6667
95-percentile: 1
Dissimilarity calculated from data file: Klon BTC 60 (Data Biner).var (type:'single') User selection Units: 30/30 and Variables: 13/13
Dissimilarity index: Presence / Absence - Dice Missing data options:
Integer code for missing data = 999
Pairwise variable deletion - Remove variables with missing data for the current unit pair (50 % of valid data required for each unit pair)
