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Stability of Lysozyme in Aqueous Extremolyte Solutions during Heat Shock and Accelerated Thermal Conditions - Ubaya Repository

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Figure 1. Molecular structure of the extremolytes betaine,hydroxyectoine, trehalose, ectoine, and mannosylglycerate(firoin).doi:10.1371/journal.pone.0086244.g001
Figure 3. The effect of extremolytes on the Nile Red fluorescence of the lysozyme-nile red complex after stressing lysozyme at 706for 10 minutes (heat shock) in citrate buffer pH 5.0.lysozyme A: without extremolytes, and with extremolyte B: betaine, C: hydroxyectoine, D: trehalose, E: ectoine, and F: firoin.C Fluorescence spectra recorded on unstressed (solid line) and stressed (dotted line)doi:10.1371/journal.pone.0086244.g003
Figure 5. The effect of extremolytes on the bioactivity oflysozyme during 4 weeks of storage at 55thermal condition).6C (accelerateddoi:10.1371/journal.pone.0086244.g005
Figure 7. The effect of firoin on the heat capacity of lysozyme determined by ITC. Firoin was titrated into lysozyme solution at thetemperatures of 10uC (&), 25uC (%), or 55uC (#).doi:10.1371/journal.pone.0086244.g007

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