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ABSTRAK
RINI HAYATI HERMAN : Aktivitas Antioksidan pada Soyghurt dengan Penambahan Ekstrak Daun Sambung Nyawa (Gynura procumbens) ( Soy-Nuraghurt) terhadap Mencit Penderita Kanker dibimbing oleh HERLA RUSMARILIN dan LINDA MASNIARY LUBIS.
Penelitian ini dilakukan untuk mengetahui pengaruh pemberian produk
soy-nuraghurt yang dibuat dengan penambahan sari sirsak, ekstrak daun sambung nyawa dan zat penstabil terhadap kesehatan mencit penderita kanker yang diinduksi dengan benzo[a]piren. Soy-nuraghurt dianalisis dengan aktivitas antioksidan, total padatan, kadar air, kadar abu, kadar protein, kadar lemak, kadar serat kasar, total asam, total bakteri asam laktat, kadar vitamin C, viskositas, total gula, total soluble solid, dan uji organoleptik warna, aroma, rasa dan tekstur. Pengujian in vivo digunakan 36 ekor mencit putih jantan yang dibagi 6 kelompok yaitu P0 (kontrol), P1 (benzo[a]piren), P2 (ekstrak), P3 (soy-nuraghurt), P4 (benzo[a]piren + ekstrak), P5 (benzo[a]piren + soy-nuraghurt. Parameter yang dianalisa adalah berat badan, histopatologi hepar dan aktivitas enzim SGPT (Serum Glutamic Pyruvate Transaminase).
Hasil penelitian menunjukkan bahwa perbandingan sari sirsak dengan ekstrak daun sambung nyawa memberikan pengaruh berbeda sangat nyata terhadap aktivitas antioksidan, kadar abu, kadar protein, kadar lemak, kadar serat kasar, total asam, total bakteri asam laktat, kadar vitamin C, nilai viskositas, total gula, total soluble solid, nilai hedonik warna, nilai hedonik aroma, dan nilai hedonik rasa. Perbandingan carboxy methyl cellulose (CMC) dengan gum arab memberikan pengaruh berbeda sangat nyata terhadap aktivitas antioksidan, total padatan, kadar air, kadar abu, kadar protein, kadar lemak, kadar serat kasar, total asam, total bakteri asam laktat, kadar vitamin C, nilai viskositas, total soluble solid, nilai hedonik aroma, nilai hedonik rasa dan nilai hedonik tekstur. Interaksi antara kedua faktor memberikan pengaruh berbeda sangat nyata terhadap kadar protein, kadar lemak, kadar serat kasar, total asam, total bakteri asam laktat, viskositas, total gula dan total soluble solid.
Perbandingan sari sirsak dengan ekstrak daun sambung nyawa (60% : 40%) dan perbandingan carboxy methyl cellulose (CMC) dengan gum arab (1 : 2) dipilih untuk pengujian in vivo (sebagai produk terbaik). Pengujian in vivo
menunjukkan bahwa pemberian soy-nuraghurt pada mencit yang diinduksi benzo[a]piren dapat memberikan efek mencegah, mengurangi dan mengobati terhadap hepar mencit, menurunkan dan menormalkan kembali aktivitas enzim SGPT (Serum Glutamic Pyruvate Transaminase) dan menunjukkan perbedaan yang signifikan terhadap berat badan mencit.
Kata kunci : Antioksidan, Benzo[a]piren, Daun sambung nyawa, Histopatologi hepar, Kanker, Serum Glutamic Pyruvate Transaminase, Soy-nuraghurt, Zat penstabil.
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ABSTRACT
RINI HAYATI HERMAN : Antioxidants Activity of soyghurt with Sambung Nyawa leaves Extract (Gynura procumbens) (Soy-Nuraghurt) on cancer suffered mice, supervised by HERLA RUSMARILIN and LINDA MASNIARY LUBIS.
This research was conducted to determine the effect of soy-nuraghurt which had been made by soursop extract, sambung-nyawa leaves extract, and stabilizers on mice’s health which had suffered from cancer induced by benzo[a]pyrene. Soy-nuraghurt were analyzed by antioxidant activity, total solids content, water content, ash content, protein content, fat content, crude fiber content, total acid,total lactic acid bacteria, vitamin C content, viscosity, total glucose, total soluble solid and organoleptic tests of color, aroma, flavor and texture. In vivo analysis was using 36 male mice who were divided into six groups, i.e : P0 (control), P1 (benzo[a]pyrene), P2 (extract), P3 (soy-nuraghurt),
P4 (benzo[a]pyrene + extract), P5 (benzo[a]pyrene + soy-nuraghurt). In vivo
analyzed were weight, histopathology of hepar, and activity of Serum Glutamic Pyruvate Transaminase enzyme.
The results showed that the ratio of soursop extract and sambung nyawa leaves extract had highly significant effect on activity antioxidant, ash content, protein content, fat content, crude fiber content, total acid, total lactic acid bacteria, vitamin C content, viscosity, total glucose, the hedonic value of the color, aroma and flavor. Ratio of carboxy methyl cellulose (CMC) and arabic gum had highly significant effect on activity antioxidant, solid content, water content, ash content, protein content, fat content, crude fiber content, total acid, total lactic acid bacteria, vitamin C content, viscosity, total soluble solid, the hedonic value of the aroma, flavor and texture. The interaction between two factors had highly significant effect on protein content, fat content, crude fiber content, total acid, total lactic acid bacteria, viscosity, total glucose and total soluble solid.
Ratio of soursop extract and sambung-nyawa leaves of (60%:40%) and ratio of carboxy methyl cellulose (CMC) and arabic gum of (1:2) had used for in vivo analysis (the best product quality). In vivo analysis showed that soy-nuraghurt benzo[a]pyrene inducted mice could give the preventive effects, decreased and treated the mice’s hepar, decreased and normalized the activity of Serum Glutamic Pyruvate Transaminase enzyme and showed highly significant effect on the mice weight.
Keywords : Antioxidants, Benzo[a]pyrene, Sambung nyawa leaves, Histophatology of hepar, Cancer, Serum Glutamic Pyruvate Transaminase, Soy-nuraghurt, Stabilizers.
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