• Tidak ada hasil yang ditemukan

The Expression of hsa-miR-155-5p in Plasma Samples Of Breast Cancer Before And After Chemotherapy | Fitria | Journal of the Medical Sciences (Berkala ilmu Kedokteran) 13678 38331 1 PB

N/A
N/A
Protected

Academic year: 2017

Membagikan "The Expression of hsa-miR-155-5p in Plasma Samples Of Breast Cancer Before And After Chemotherapy | Fitria | Journal of the Medical Sciences (Berkala ilmu Kedokteran) 13678 38331 1 PB"

Copied!
1
0
0

Teks penuh

(1)

Supplement J Med Sci, Volume 48, No. 4, 2016 October

20

The expression of Hsa-miR-155-5p in

plasma samples of breast cancer before

and after chemotherapy

Meutia Srikandi Fitria1*, Sofia Mubarika Haryana2, Sumadi Lukman Anwar3,

Teguh Aryandono2, Dewi Sahfitri Tanjung1, Aprilia Indra Kartika1, Risky Oktriani4,

Irianianiwati5, Dwi Nur Indah Sari6

1Graduate Program in Biotechnology, School of Postgraduate, Universitas

Gadjah Mada, Yogyakarta, 2Doctoral Program, Faculty of Medicine, Universitas

Gadjah Mada, Yogyakarta, 3Dr. Sardjito General Hospital, Yogyakarta, Indonesia, 4Department of Biochemistry, Faculty of Medicine, Universitas Gadjah Mada,

Yogyakarta, 5Department of Patology Anatomi, Faculty of Medicine, Universitas

Gadjah Mada, Yogyakarta, 6Graduate Program in Biomedicine, Faculty of Medicine,

Universitas Gadjah Mada

DOI: http://dx.doi.org/10.19106/JMedScieSup0048042016018

ABSTRACT

Breast cancer has emerged as the most common cancer-related mortality among

women worldwide. Therefore, early cancer detection using biomarkers such as

microRNA is required. One of microRNAs that has an important role in breast cancer development is miR-155. Hsa-miR-155-5p is an oncomiR that is commonly

dysregulated in breast cancer. This study aims to determine the expression of

hsa-miR-155-5p in breast cancer patient’s plasma before and after chemotherapy. We collected 64 samples from breast cancer patients admitted to Dr. Sardjito Hospital in Yogyakarta. RNA from plasma was extracted using RNA Isolation

Kit miRCURY-Bioluid. cDNA synthesis was performed using cDNA Synthesis kit II and quantiication of miR-155-5p using ExiLent SYBR Green master mix (Exiqon). qRT-PCR results were then analyzed with Livak’s method and compared

(before and after chemotherapy) to t-test. Expression of miR-155-5p in the breast

cancer patients’ plasma after chemotherapy was signiicantly increased (10.59

times) when compared to before chemotherapy (p = 0.001). We concluded that there was upregulated expression of miR-155-5p after chemotherapy than before

chemotherapy. There has not been a known, relevant pathway between

hsa-miR-155-5p and chemotherapy regimens nor its impact on resistance to chemotherapy.

Keywords: Breast cancer, plasma, hsa-miR-155-5p, oncomiR, chemotherapy.

Referensi

Dokumen terkait

Alhamdulillahirobbil’alamin segala puji dan syukur penulis panjatkan kehadirat Allah SWT yang telah melimpahkan rahmat dan karunia-Nya sehingga penulis dapat menyelesaikan

Variabel yang digunakam dalam penelitian adalah variabel bebas yaitu menggunakan posisi netral dan Abduction and External Rotation dengan sekuen Gradient Echo

Namun dengan status batik yang masih merupakan industri lokal dan belum penuh memasuki industri global, maka masyarakat pun banyak yang berfikir kalau pemakaian

Barangsiapa terlibat peristiwa kecelakaan lalu lintas pada waktu mengemudikan kendaraan bermotor di jalan dan tidak menghentikan kendaraannya, tidak menolong orang yang menjadi

Tabel kategori digunakan untuk menampung dan menyimpan data berbagai kategori produk yang natinya akan mengelompokkan produk berdasarkan kategori. Berikut ini adalah field-field

[r]

Organisme tanah yang lebih besar dapat memperbaiki struktur tanah dengan cara membuat saluran-saluran (lubang-lubang) di dalam tanah (contohnya lubang cacing), dan

1.OBAT UTAMA, YAITU OBAT KRONIS YANG DIRESEPKAN OLEH DOKTER SPESIALIS/SUB SPESIALIS DI FASKES TINGKAT LANJUTAN. 2.OBAT TAMBAHAN, YAITU OBAT YANG MUTLAK DIBERIKAN BERSAMA OBAT