ﻪﻠﺠﻣ مﻮﻠﻋ يزارﻲﻜﺷﺰﭘ هرود 18 ، هرﺎﻤﺷ 91 ، يد 1390 ﻲﺸﻫوﮋﭘ ﻪﻟﺎﻘﻣ ﻪﻣﺪﻘﻣ رد تادﻮﺟﻮﻣ ﺮﭘ ﻲﻟﻮﻠﺳ ﻪﻛ لﻮﻠﺳ ﺎـﻫ ﻲﻳﺎـﻧاﻮﺗ ﻢﻴـﺴﻘﺗ ،ﺪﻧراد يﺮﻴﮔﻮﻠﺟ زا ﺮﻴﺜﻜﺗ ﻲـﺑ ﻪـﻳور نآ ﺎـﻫ رﺎﻴـﺴﺑ ﺰﺋﺎـﺣ ﺖﻴﻤﻫا ﺖﺳا . ﻪﺑ مﺎﮕﻨﻫ زوﺮـﺑ ﻚـﻳ ﺶﻨـﺗ ﺮـﻴﻈﻧ رﻮـﻀﺣ يﺎــﻫﻮﺗﺮﭘ ناﺰﻴﻧﻮــﻳ ﻪــﻛ رد نآ DNA رﺎــﭼد ﻪﻣﺪــﺻ ﻲﻣ ،ددﺮﮔ يﺎﻫ نژ ﺮﮕﺑﻮﻛﺮـﺳ رﻮـﻣﻮﺗ ﺮـﻴﻈﻧ p53 ، لﺎـﻌﻓ هﺪﺷ و ناﺰﻴﻣ نﺎﻴﺑ نآ ﺶﻳاﺰﻓا ﻲﻣ ﺪﺑﺎﻳ . ﻦﻴﺌﺗوﺮــﭘ p53 ﻪــﻛ ﻦﻴﺌﺗوﺮــﭘ يرﻮــﻣﻮﺗ ) Tp53 ( ﺰــﻴﻧ هﺪﻴﻣﺎﻧ ﻲﻣ ،دﻮﺷ ﻢﻴـﻈﻨﺗ هﺪـﻨﻨﻛ ﻪـﺧﺮﭼ ﻲﻟﻮﻠـﺳ هدﻮـﺑ و ﻲﻣ ناﻮﺗ نآ ار ﻒـﻗﻮﺘﻣ هﺪـﻨﻨﻛ رﻮـﻣﻮﺗ ﻢـﻫ ﺪـﻴﻣﺎﻧ ) 1 و 2 .( ناﺰﻴﻣ p53 رد لﻮﻠﺳ يﺎﻫ ﻲﻌﻴﺒﻃ ﻢﻛ و ﻪﺑ رﻮﻃ ﻪﺘـﺳﻮﻴﭘ p53 يزﺎﺳزﺎﺑ DNA ار ﻢﻴﻈﻨﺗ ﻲﻣ ،ﺪﻨﻨﻛ ﻞﺼﺘﻣ هﺪﺷ و گﺮﻣ ﻪﻣﺎﻧﺮﺑ يﺰﻳر هﺪﺷ لﻮﻠﺳ ار لﺮﺘﻨﻛ ﻲﻣ ﺪﻨﻛ ) 6 -3 .( ﺎﻣا ﻞﻜﺸﻣ ﺖﺳﺎﺠﻨﻳا ﻪﻛ رد لﻮﻠﺳ يﺎﻫ ،ﻲﻧﺎﻃﺮﺳ دﻮـﺧ نژ p53 ﺰــﻴﻧ ﻦــﻜﻤﻣ ﺖــﺳا ﺮــﻴﻴﻐﺗ ﻪــﺘﻓﺎﻳ و ﺮــﻴﻏ لﺎــﻌﻓ ددﺮﮔ ) 7 و 8 .( تﺎﻘﻴﻘﺤﺗ نﺎـﺸﻧ ﻲـﻣ ﺪـﻨﻫد ﻪـﻛ ًادوﺪـﺣ رد % 50 زا ﻲﻣﺎﻤﺗ نﺎﻃﺮﺳ ﺎﻫ p53 ﺮﻴﻴﻐﺗ اﺪـﻴﭘ هدﺮـﻛ ﺖـﺳا ) 11 -9 (. نﺎﺼﺼﺨﺘﻣ ﻚـﻴﺘﻧژ ﺪـﻧﺪﻘﺘﻌﻣ ﺮـﮔا نژ p53 رد لﻮﻠﺳ يﺎﻫ ﻲﻧﺎﻃﺮﺳ لﺎﻌﻓ ،دﻮـﺷ ﺪـﺷر ﻦـﻳا لﻮﻠـﺳ ﺎـﻫ ﻒﻗﻮﺘﻣ ﻲـﻣ ددﺮـﮔ . ﻦـﻳا ،ﺮـﻴﻴﻐﺗ ﺖـﺻﺮﻓ ﻲﺒـﺳﺎﻨﻣ ياﺮـﺑ ﻢﺘﺴﻴﺳ ﻲﻨﻤﻳا ﻢﻫاﺮﻓ ﻲﻣ دروآ ﺎﺗ لﻮﻠﺳ يﺎﻫ ﻲﻧﺎﻃﺮـﺳ ار زا ﻦﻴﺑ دﺮﺒﺑ ) 12 و 13 ( . DNA * ﻼﻬﺷﺮﺘﻛد يﺪﻤﺤﻣ : رﺎﻳدﺎﺘﺳا ﺺﺼﺨﺘﻣو ﻚﻳﺰﻴﻓﻮﻴﺑ هﺪﻜﺸﻫوﮋﭘ،ﻲﻟﻮﻜﻟﻮﻣ مﻮﻠﻋ ﻪﺘﺴﻫ هﺎﮕﺸﻫوﮋﭘ،يا مﻮﻠﻋ و نﻮﻨﻓ ﻪـﺘﺴﻫ ناﺮـﻬﺗ،يا -ناﺮﻳا *) لوﻮﺌﺴﻣﻒﻟﻮﻣ (. smohammadi@aeoi.org.ir ﺪﻤﺤﻣ ﺎﺿر ﻲﺘﺋاﺮﻗ : هروديﻮﺠﺸﻧاد ﻲﺼﺼﺨﺗيﺮﺘﻛد هﺪﻜﺸﻧاد،ﻲﻤﻴﺷﻮﻴﺑ هﺎﮕﺸﻧاد،مﻮﻠﻋ ﺖﻴﺑﺮﺗ ،سرﺪﻣ ناﺮﻳا،ناﺮﻬﺗ . gharaati@modares.ac.ir يﺮﺒﻛ ﻲﻛز نﺎﺧ -ﮓﻨﻟا : هروديﻮﺠﺸﻧاد ﻲﻜﺷﺰﭘﻲﺼﺼﺨﺗيﺮﺘﻛد هﺪﻜﺸﻧاد،ﻲﻟﻮﻜﻟﻮﻣ مﻮﻠﻋ ﻪﻳﺎﭘ هﺎﮕﺸﻧاد،ﻲﻜﺷﺰﭘ مﻮﻠﻋ ﻲﻜﺷﺰﭘ ناﺮﻳا،ﻦﻳوﺰﻗ،ﻦﻳوﺰﻗ . ezakikhan@yahoo.com ﺎﺳر ﻲﻳﺎﺟر : ﻲﺳﺎﻨﺷرﺎﻛ ﺪﺷرا ﺖﺴﻳز ﻲﻟﻮﻠﺳ -هﺪﻜﺸﻫوﮋﭘ،ﻲﻟﻮﻜﻟﻮﻣ دﺮﺑرﺎﻛ هﺎﮕﺸﻫوﮋﭘ،ﺎﻫﻮﺗﺮﭘ مﻮﻠﻋ و نﻮﻨﻓ ﻪـﺘﺴﻫ ،يا ناﺮﻳا،ناﺮـﻬﺗ . rrajaie@aeoi.org.ir
ﻪﺴﻳﺎﻘﻣ
زوﺮﺑ
ﻦﻴﺌﺗوﺮﭘ
p53رد
هوﺮﮔ
نارﺎﻛﻮﺗﺮﭘ
ﺎﺑ
هوﺮﮔ
ﺪﻫﺎﺷ
هﺪﻴﻜﭼ فﺪﻫوﻪﻨﻴﻣز : يﺎﻫﺶﻨﺗ نﻮﮔﺎﻧﻮﮔ ﺮﻴﻈﻧ يﺎﻫﻮﺗﺮﭘ ناﺰﻴﻧﻮﻳ ﻲﻣ ﺪﻨﻧاﻮﺗ ﺐﺟﻮﻣ ﺶﻳاﺰﻓا يﺎﻫﺐﻴﺳآ ﻲﻟﻮﻠﺳ صﻮﺼﺧﻪﺑ رد ﺶﺨﺑ ﻪﺘـﺴﻫ نآ ﻲـﻨﻌﻳ DNA ﺪﻧدﺮﮔ . ياﺮﺑ ﺖﻈﻓﺎﺤﻣ زا ﻦﻳا ﻪﻧﻮﮔ ،ﺎﻫﺐﻴﺳآ نژ يﺎﻫ ﻲﻤﻴﻈﻨﺗ ﻲﻌﻴﺒﻃ ﺪﻨﻧﺎﻣ نژ بﻮﻛﺮﺳ هﺪﻨﻨﻛ رﻮﻣﻮﺗ Tp53 دراو ﻞﻤﻋ ﻲﻣ ﺪﻧدﺮﮔ . ﻦﻳارد ،طﺎﺒﺗرا رد ﻦﻴﺑ ﻦﻴﻠﻏﺎﺷ يرﺎﻛﻮﺗﺮﭘ ﻪﻛ هراﻮﻤﻫ ﺎﺑ يﺎﻫزود ﻲﻨﻴﻳﺎﭘ زا يﺎﻫﻮﺗﺮﭘ ناﺰﻴﻧﻮﻳ رﺎﻛوﺮﺳ ﺪﻧراد ﻦﻳا، نژ ﻲﺳرﺮﺑ و ناﺰﻴﻣ زوﺮﺑ نآ ﺎﺑ هوﺮﮔ لﺮﺘﻨﻛ ﻪﺴﻳﺎﻘﻣ ﺪﺷ . شور رﺎﻛ : ﺎﺑ،ﻲﻠﻴﻠﺤﺗﻪﻌﻟﺎﻄﻣﻦﻳارد هدﺎﻔﺘﺳا زا ﺖﻴﻛ ،اﺰﻳﻻا راﺪﻘﻣ ﻦﻴﺌﺗوﺮﭘ p53 مﺮﺳ ﻲﻧﻮﺧ رد 42 ورﺎـﻛﻮﺗﺮﭘدﺮﻓ 16 هزاﺪـﻧاﺪﻫﺎـﺷدﺮـﻓ يﺮـﻴﮔ ﺪـﺷ . ﻲﺳرﺮﺑ يﺎﻫ يرﺎﻣآ ﻂﺳﻮﺗ شور student t-test مﺎﺠﻧا ﺖﻓﺮﮔ . ﻪﺑ ناﻮﻨﻋ هوﺮﮔ رﺎﻛﻮﺗﺮﭘ زا ﻦﻴﻠﻏﺎﺷ نﺎﻣزﺎﺳ يژﺮﻧا ﻲـﻤﺗا ناﺮـﻳا ) ﺎـﺑ ﻦﻴﮕﻧﺎـﻴﻣ ﻲﻨـﺳ 3 / 1 ± 8 / 37 لﺎﺳ ( تﻮﻋد ﺪﺷيرﺎﻜﻤﻫﻪﺑ . ﻪﺑ ناﻮﻨﻋ هوﺮﮔ ﺪﻫﺎﺷ ﺰﻴﻧ ﺮﻈﻧزاﻪﻛﻲﻨﻴﺒﻠﻃواد ،ﻦﺳ ﺲﻨﺟ و تادﺎﻋ رﺎﮕﻴﺳ نﺪﻴﺸﻛ ﺎﺑ هوﺮﮔ رﺎﻛﻮﺗﺮﭘ ﻖﺑﺎﻄﺗ ﺪﻨﺘﺷاد ) ﺎـﺑ ﻦﻴﮕﻧﺎﻴﻣ ﻲﻨﺳ / 2 ± 5 / 34 لﺎﺳ ( ، ﺪﻧﺪﻳدﺮﮔبﺎﺨﺘﻧا . زا 2 ﻲﺳﻲﺳ ﺪﺷجاﺮﺨﺘﺳاژﻮﻔﻳﺮﺘﻧﺎﺳﻂﺳﻮﺗنآمﺮﺳ،دﺮﻓﺮﻫﻪﻨﻳرﺎﭙﻫنﻮﺧ . ﻪﺘﻓﺎﻳ ﺎﻫ : نژنﺎﻴﺑ p53 ﺑرﺎﻛﻮﺗﺮﭘهوﺮﮔرد ﻪ ﻲﻨﻌﻣرﻮﻃ ﺪﻫﺎﺷهوﺮﮔزاﺮﺗﻻﺎﺑيراد دﻮﺑ ) 0225 / 0 = p .( هوﻼﻋ ﺮﺑ ،ﻦﻳا ﻪﻘﺑﺎﺳ رﺎﻛ رﺎﻛﻮﺗﺮﭘ و ﺎﻳ فﺮـﺼﻣ رﺎﮕﻴـﺳ يﺮﻴﺛﺎﺗ ﺮﺑ ناﺰﻴﻣ نﺎﻴﺑ ﻦﻳا نژ ﻪﺘﺷاﺪﻧ ﺖﺳا . ﻪﺠﻴﺘﻧ يﺮﻴﮔ : يﺎﻫزود ﺮﻤﺘﺴﻣوﻦﻴﻳﺎﭘ يﺎﻫﻮﺗﺮﭘ ناﺰﻴﻧﻮﻳ ردﻲﻳاﺰﺳﻪﺑﺶﻘﻧ ﺶﻳاﺰﻓا نﺎﻴﺑ نژ p53 رد ﻦﻴﺑ ﻦﻴﻠﻏﺎﺷ رﺎﻛﻮﺗﺮﭘ ﺖﺳاﻪﺘﺷاد . ردﻦـﻳاو ﻲﻟﺎـﺣ ﻪﻛﺖﺳا ﺮﺑ سﺎﺳا ﺞﻳﺎﺘﻧ ﺖﺒﺛ هﺪﺷ رد يﺮﺘﻤﻳزود يدﺮﻓ ﻦﻳا ،داﺮﻓا زود ﻲﺘﻓﺎﻳرد رد هدوﺪﺤﻣ زود زﺎﺠﻣ ﻪﻧﻻﺎﺳ ياﺮﺑ نارﺎﻛﻮﺗﺮﭘ هدﻮﺑ ﺖﺳا . ﻦﻳا ﻪﻠﺌـﺴﻣ ﺪـﻳﻮﻣ ﻦﻳا عﻮﺿﻮﻣ ﺖﺳا ﻪﻛ ﺶﻳﺎﭘ نارﺎﻛﻮﺗﺮﭘ ﺪﻳﺎﺒﻧ ﺎﻬﻨﺗ ﺮﺑ سﺎﺳا يﺮﺘﻤﻳزود يﺎﻫ ﻲﻜﻳﺰﻴﻓ ﻪﻜﻠﺑ مزﻻ ﺖﺳا ﺎـﺗ زا ﺺﺧﺎـﺷ يﺎـﻫ ﻲﻜﻳژﻮـﻟﻮﻴﺑ ﻪـﻛ ردﺎـﻗ ﻪـﺑ ﻪـﺋارا ﺐﻴﺳآ يﺎﻫ يﻮﺗﺮﭘ يدﺮﻓ هدﻮﺑ ﺰﻴﻧ هدﺎﻔﺘﺳا دﻮﺷ . هژاوﺪﻴﻠﻛ ﺎﻫ : زوﺮﺑ ﻦﻴﺌﺗوﺮﭘ p53 ، ﻮﺗﺮﭘ ،ناﺰﻴﻧﻮﻳ ﺐﻴﺳآ ﻲﻟﻮﻠﺳ ﺖﻓﺎﻳردﺦﻳرﺎﺗ : 10 / 2 / 90 شﺮﻳﺬﭘﺦﻳرﺎﺗ : 19 / 10 / 90ﻪﺴﻳﺎﻘﻣ زوﺮﺑ ﻦﻴﺌﺗوﺮﭘ p53 رد هوﺮﮔ نارﺎﻛﻮﺗﺮﭘ ... 37 ﻞﻜﺷ 1 . ﻲﻨﺤﻨﻣ دراﺪﻧﺎﺘﺳا ﻦﻴﺌﺗوﺮﭘ p53 نﺎﻴﺑ نژ p53 ﻻﺎﺑ ﻪﺘﻓر و لﻮﻠـﺳ رد ﻦـﻳا مﺎـﮕﻨﻫ ود هار ﺶﻴﭘ يور ؛دراد هار لوا ﻦﻳا ﺖﺳا ﻪـﻛ ﺐﻴـﺳآ هدراو ﻪـﺑ DNA ﺮﻴﻤﻌﺗ هﺪﺷ و هار مود ﻦﻳا ﻪـﻛ ﺮـﮔا ﺐﻴـﺳآ هدراو ﺮﻴﻤﻌﺗ ،ددﺮﮕﻧ لﻮﻠﺳ گﺮﻣ ﻪﻣﺎﻧﺮﺑ يﺰﻳر هﺪـﺷ ) زﻮـﺘﭘﻮﭘآ ( ار رد ﺶﻴﭘ دﺮﻴﮔ . رد ﻊﻗاو ﺦﺳﺎﭘ p53 ﻦﻴـﻴﻌﺗ ﻲـﻣ ﺪـﻨﻛ ﻪـﻛ لﻮﻠﺳ هار ﺗ ﺮﻴﻤﻌ DNA ار ﺶﻴﭘ دﺮﻴﮔ ﺎﻳ گﺮﻣ يزﻮﺘﻴﻣ ار لﺎﻌﻓ ﺪﻨﻛ ) 14 و 15 .( ﺎﺑ ﻪﺟﻮﺗ ﻪﺑ ﺐﻟﺎﻄﻣ ﺮﻛذ هﺪﺷ ناﺰﻴﻣ نﺎﻴﺑ نژ p53 ياﺮﺑ ﻲﻨﻤﻳا داﺮﻓا يرﺎﻛﻮﺗﺮﭘ ﻪﻛ ﻪﺑ ظﺎﺤﻟ ﻲﻠﻐﺷ رد ضﺮﻌﻣ ﻮﺗﺮﭘ راﺮﻗ ﺪﻧراد زا ﺖﻴﻤﻫا ﻲﻳاﺰﺴﺑ ﺮﺑ رادرﻮﺧ ﺖﺳا . ،اﺬﻟ رد ﻦﻳا ﻖﻴﻘﺤﺗ ﻲﻫوﺮﮔ زا نارﺎﻛﻮﺗﺮﭘ ﻪﻛ رد ضﺮﻌﻣ ﻢﺋاد ﺎﻳ ﺖﻗﻮﻣ يﺎﻫزود ﺮﻴﻐﺘﻣ زا يﺎﻫﻮﺗﺮﭘ نﻮﻳ زﺎـﺳ راﺮـﻗ ﻪﺘﻓﺮﮔ ﺪﻧا ﻪﺑ ناﻮﻨﻋ ﻚﻳ لﺪﻣ ﻲﺘﻴﻌﻤﺟ رﺎـﻛﻪـﺑ وﻪـﺘﻓﺮﮔ ناﺰﻴﻣ نﺎﻴﺑ نژ p53 رد ﻦﻳا هوﺮﮔ ﺎـﺑ ﻚـﻳ هوﺮـﮔ ﺪﻫﺎـﺷ ﺪﻳدﺮﮔﻪﺴﻳﺎﻘﻣ . شور رﺎﻛ هزاﺪﻧا يﺮـﻴﮔ ناﺰـﻴﻣ نﺎـﻴﺑ ﻦﻴﺌﺗوﺮـﭘ p53 ﻪـﺑ ﻚـﻤﻛ ﺶﻳﺎﻣزآ ياﺰﻳﻻا p53 ﻂـﺳﻮﺗ ﺖـﻴﻛ يراﺪـﻳﺮﺧ هﺪـﺷ زا ﺖﻛﺮـﺷ Bender MedSystems (BMS256 مﺎـﺠﻧا ﻪﺘﻓﺮﮔ ﺖﺳا . رﻮﻃ ﻪﺑ ﻲﻠﻛ ياﺮﺑ مﺎـﺠﻧا ﻦـﻳا نﻮـﻣزآ و ﺎـﺑ ﻪﺟﻮﺗ ﻪﺑ شور رﺎﻛ ﻦـﻳا ،ﺖـﻴﻛ ﻲــﻃ ﻦﻳﺪــﻨﭼ ﻪــﻠﺣﺮﻣ ﻮﺸﺘﺴﺷ ﺎﺑ يﺎـﻫ لﻮﻠﺤﻣ ،ﻒﻠــﺘﺨـﻣ ﺶﻨـﻛاو ﻲﻳﺎـﻬﻧ رد ﺐﻟﺎﻏ ﻚـﻳ لﻮﻠﺤﻣ ﻲﮕﻧر ﻞﺻﺎﺣ ﺖﺳد ﻪﺑ ﻲﻣ ﺪـﻳآ ﻪــﻛ تﺪـــﺷ ﮓــﻧر نآ ﺐــﺳﺎﻨﺘﻣ ﺎـــﺑ راﺪـــﻘﻣ p53 رد ﻪــﻧﻮﻤﻧ ﺖـﺳا . ياﺮﺑ ﺮﻫ دﺮﻓ ود ﻪﻧﻮﻤﻧ ﻪﻴﻬﺗ و ﺶﻳﺎﻣزآ اﺰـﻳﻻا ﺮـﺑ يور نآ مﺎﺠﻧا ﻪﺘﻓﺮﮔ و ﻦﻴﮕﻧﺎﻴﻣ ود ﺶﻳﺎـﻣزآ ﻪـﺑ ناﻮـﻨﻋ ﺞﻳﺎﺘﻧ ﻪﻌﻟﺎﻄﻣ ﻪﺋارا ﺪﺷ . ﻪﺑ رﻮﻈـﻨﻣ ﻦـﻴﻴـﻌﺗ راﺪــﻘﻣ p53 ، بﺬــــﺟ لﻮــــﻠﺤﻣ رد 450 ﺮﺘﻣﻮﻧﺎﻧ ﻂــــﺳﻮﺗ هﺎﮕﺘــــﺳد ناﻮــــﺧاﺰﻳﻻا Anthos ) لﺪــــﻣ 2020 Microplate Reader ( هزاﺪﻧا ﻪﺘﻓﺮﮔ ﺪﺷ . ﻲﺳرﺮﺑ يﺎﻫ يرﺎﻣآ ﻪﺴﻳﺎﻘﻣو ناﺰﻴﻣ نﺎﻴﺑ ﻦﻴﺌﺗوﺮﭘ p53 ﻦﻴــﺑ ود هوﺮــﮔ ﺪﻫﺎــﺷ و رﺎــﻛﻮﺗﺮﭘ ﺎــﺑ هدﺎﻔﺘــﺳا زا شور student t-test مﺎــﺠﻧا ﺖــﻓﺮﮔ . ﺖــﻬﺟ ﻲــﺳرﺮﺑ ﺮﻴﺛﺎــﺗ ﺮﻴﻐﺘﻣ يﺎﻫ ﻪﻘﺑﺎﺳ رﺎﻛ تﺎـﻴﻧﺎﺧدفﺮـﺼﻣوﻦـﺳ ﻦﻴـﺑرد ﺖـﺴﺗزا،رﺎﻛﻮﺗﺮﭘهوﺮﮔ
one way ANOVA هدﺎﻔﺘـﺳا ﺪﻣآﻞﻤﻋﻪﺑ . نﺎﻴﺑناﺰﻴﻣﻦﻴﺑﻲﮕﺘﺴﺒﻤﻫﻦﻴﻴﻌﺗرﻮﻈﻨﻣﻪﺑ ﻲﮕﺘـﺴﺒﻤﻫنﻮـﻣزآ ،نارﺎـﻛﻮﺗﺮﭘيﻮﺗﺮﭘﻪﻘﺑﺎﺳ وﻦﻴﺌﺗوﺮﭘ ﺪﺷﻪﺘﻓﺮﮔ رﺎﻛﻪﺑ نﻮﺳﺮﻴﭘ . زاهدﺎﻔﺘﺳا ﺎﺑيرﺎﻣآيﺎﻫﺰﻴﻟﺎﻧآ راﺰﻓامﺮﻧ Origin 6.1 و Excel ﻢﻴـﺳﺮﺗوﻪﺘﻓﺮﮔ مﺎﺠﻧا ﺪﻧﺪﻳدﺮﮔ . ﻞﻨﺳﺮﭘ درﻮﻣ ﻪـﻌﻟﺎﻄﻣ رد ﻦـﻳا هژوﺮـﭘ زا ﻦﻴـﺑ ﻲﻧﺎـﺒﻠﻃواد بﺎﺨﺘﻧا هﺪﺷ ﺪﻧا ﻪﻛ رد نﺎﻣزﺎﺳ يژﺮـﻧا ﻲـﻤﺗا ناﺮـﻳا ﻪـﺑ يﺎﻫ ﺖﻴﻟﺎﻌﻓ نﻮﮔﺎﻧﻮﮔ يﻮﺗﺮﭘ لﺎﻐﺘﺷا ﺪﻧراد . ﻦﻳا داﺮـﻓا ﺎـﺑ ﻪﺟﻮﺗ ﻪﺑ ﻞﺤﻣ ،رﺎﻛ تﺪﻣ نﺎﻣز رد ضﺮـﻌﻣ ﻮـﺗﺮﭘ ،ندﻮـﺑ ﻪﻘﺑﺎﺳ رﺎﻛ و ﺖﻴﻌﺿو ﻲﺘﻣﻼـﺳ بﺎـﺨﺘﻧا هﺪـﻳدﺮﮔ ﺪـﻧا . رد عﻮﻤﺠﻣ 42 دﺮﻓ رﺎﻛﻮﺗﺮﭘ رد ﻦﻳا رﺎـﻛ ﻲﺗﺎـﻘﻴﻘﺤﺗ ﺖﻛﺮـﺷ ﺪﻧدﺮﻛ . ﺎﺑ ﻪﺟﻮﺗ ﻪﺑ هﺪﻧوﺮﭘ ﻲﻜﺷﺰﭘ ﻦﻳا ﻪـﺑ ﺞﻳﺎـﺘﻧوداﺮـﻓا ،ﺎﻫ نآ ﻲﻜﻳﺰﻴﻓيﺎﻫﺮﺘﻤﻳزود زاهﺪﻣآ ﺖﺳد زود ﻲﺘﻓﺎﻳرد ﺎﻫ نآ زا زود زﺎﺠﻣ ﻪﻧﻻﺎﺳ ياﺮﺑ نارﺎﻛﻮﺗﺮﭘ زوﺎﺠﺗ هدﺮـﻜﻧ ﺖﺳا . ﻪﺘﺒﻟا تﺎﻋﻼﻃا طﻮﺑﺮﻣ ﻪﺑ يﺎﻫزود ﺖﺒﺛ هﺪـﺷ ﻦـﻳا داﺮﻓا و داﺪﻌﺗ تﺎﻋﺎﺳ رﺎﻛ داﺮﻓا ﺎﺑ ﻮﺗﺮﭘ ﺰﻴﻧ رد سﺮﺘﺳد ﻦﻳا هوﺮﮔ ﻲﺗﺎﻘﻴﻘﺤﺗ هدﻮﺒﻧ ﺖﺳا . زا ﻲـﻓﺮﻃ 16 دﺮـﻓ ﺐـﻠﻃواد ﻪﻛ زا ظﺎﺤﻟ ﻦﺳ و ﻲﺗادﺎﻋ ﺮﻴﻈﻧ لﺎﻤﻌﺘﺳا ،تﺎﻴﻧﺎﺧد ﻞـﻜﻟا و هﺮﻴﻏ ﺎﺑ هوﺮﮔ رﺎﻛﻮﺗﺮﭘ ﻖﺑﺎﻄﺗ ﻲﻜﻳدﺰﻧ ﺪﻨﺘﺷاد ﻪﺑ ناﻮـﻨﻋ هوﺮﮔ ﺪﻫﺎﺷ بﺎﺨﺘﻧا ﺪﻧﺪﺷ . زا ﻦﻳا ،داﺮﻓا ﺎﺑ ﺖﻳﺎﻋر ﻞﺋﺎـﺴﻣ ﻲـﻗﻼﺧا و نداد ﻲﻫﺎـﮔآ مزﻻ زا عﻮــﺿﻮﻣ ،ﻖــﻴﻘﺤﺗ 2 ﻲــﺳﻲــﺳ نﻮــﺧ ﻪــﻨﻳرﺎﭙﻫ ﻪــﺘﻓﺮﮔ ﺪــﺷ . ﺲﭙــﺳ ﺎــﺑ هدﺎﻔﺘــﺳا زا ژﻮﻔﻳﺮﺘﻧﺎــﺳ ) رد رود g × 250 و تﺪﻣﻪﺑ 10 ﻪﻘﻴﻗد رد يﺎﻣد 20 ﻪﺟرد ﻲﺘﻧﺎـﺳ داﺮﮔ ( ، مﺮـﺳ زا ﻪﻴﻘﺑ ياﺰـﺟا نﻮـﺧ اﺪــﺟ هﺪـﺷ و ياﺮـﺑ ﺶﻳﺎﻣزآ يﺎﻫ يﺪﻌﺑ رد 80 -ﻪﺟرد داﺮﮔ ﻲﺘﻧﺎﺳ يراﺪﻬﮕﻧ ﺪﻳدﺮﮔ . رﺎـﻛﻪﻘﺑﺎـﺳوﺖﻴـﺴﻨﺟ ،ﻦﺳﻪﺑطﻮﺑﺮﻣتﺎﻋﻼﻃا لوﺪﺟردداﺮﻓاﻦﻳايﻮﺗﺮﭘ 1 ﺖﺳاهﺪﻣآ .
ﺎﻫﻪﺘﻓﺎﻳ ياﺮﺑ ﻦﻴﻴﻌﺗ ﺖﻈﻠﻏ ﻦﻴﺌﺗوﺮـﭘ رد يﺎﻤـﺳﻼﭘ ،ﻦﻴـﺒﻠﻃواد اﺪﺘﺑا ﺖﻔﻫ ﺖﻈﻠﻏ توﺎﻔﺘﻣ زا ﻚـﻳ ﻪـﻧﻮﻤﻧ ﻦﻴﺌﺗوﺮـﭘ p53
ﺮﺘﻛد ﻼﻬﺷ يﺪﻤﺤﻣ نارﺎﻜﻤﻫ و 38 لوﺪﺟ 1 . نﺎﺒﻠﻃوادتﺎﺼﺨﺸﻣ ﻦﻴﺌﺗوﺮﭘﺖﻈﻠﻏ ) U/ml ( يﻮﺗﺮﭘرﺎﻛﻪﻘﺑﺎﺳ ﺖﻴﺴﻨﺟ/ﻦﺳ دﺮﻓهرﺎﻤﺷ (U/ml)ﻦﻴﺌﺗوﺮﭘﺖﻈﻠﻏ يﻮﺗﺮﭘرﺎﻛﻪﻘﺑﺎﺳ ﺖﻴﺴﻨﺟ/ﻦﺳ دﺮﻓهرﺎﻤﺷ 00 / 0 8 دﺮﻣ /31 1R 9/88 2 نز /31 30R 80 / 1 4 دﺮﻣ /25 2R 4/82 7 دﺮﻣ /35 31R 36 / 96 22 دﺮﻣ /46 3R 1/50 1 دﺮﻣ /24 32R 74 / 0 20 دﺮﻣ /52 4R 12/85 9 دﺮﻣ/ 35 33R 29 / 0 10 دﺮﻣ /37 5R 13/72 13 نز /35 34R 25 / 2 23 دﺮﻣ /47 6R 7/07 8 دﺮﻣ /34 35R 82 / 1 20 دﺮﻣ /41 7R 11/44 8 دﺮﻣ /49 36R 21 / 0 23 دﺮﻣ /49 8R 0/13 2 دﺮﻣ /26 37R 16 / 5 17 دﺮﻣ /51 9R 1/96 3 دﺮﻣ /30 38R 75 / 2 20 دﺮﻣ /46 10R 1/58 2 دﺮﻣ /29 39R 01 / 0 10 دﺮﻣ /34 11R 11/10 3 دﺮﻣ /29 40R 80 / 13 12 دﺮﻣ /35 12R 14/54 4 دﺮﻣ /35 41R 27 / 5 12 نز /35 13R 1/64 2 دﺮﻣ /28 42R 13 / 1 24 دﺮﻣ /51 14R 9/27 -- نز/31 1C 42 / 0 10 دﺮﻣ /34 15R 0/29 -- دﺮﻣ/44 2C 54 / 9 7 دﺮﻣ /32 16R 1/27 -- دﺮﻣ/45 3C 07 / 3 11 دﺮﻣ /35 17R 4/50 -- دﺮﻣ/25 4C 58 / 0 2 دﺮﻣ /45 18R 0/31 -- دﺮﻣ/42 5C 26 / 37 8 نز/33 19R 1/21 -- دﺮﻣ/39 6C 27 / 1 18 دﺮﻣ /48 20R 11/76 -- نز/43 7C 01 / 0 3 دﺮﻣ /37 21R 9/22 -- دﺮﻣ/27 8C 77 / 38 20 دﺮﻣ /48 22R 0/05 -- دﺮﻣ/27 9C 14 / 17 20 دﺮﻣ /46 23R 0/44 -- دﺮﻣ/35 10C 78 / 14 8 دﺮﻣ/ 39 24R 5/90 -- دﺮﻣ/30 11C 38 / 6 16 نز /48 25R 3/65 -- دﺮﻣ/28 12C 75 / 42 20 نز /52 26R 4/39 -- دﺮﻣ/51 13C 29 / 45 8 دﺮﻣ /35 27R 0/79 -- دﺮﻣ/29 14C 50 / 4 1 دﺮﻣ /27 28R 4/90 -- دﺮﻣ/36 15C 87 / 1 2 دﺮﻣ /30 29R 1/16 -- دﺮﻣ/36 16C دراﺪﻧﺎﺘـــــﺳا ) 50 ، 25 ، 5 / 12 ، 3 / 6 ، 2 / 3 ، 6 / 1 ، 8 / 0 ، 0 / 0 ﻲﺳﻲﺳ ﺮﻫ رد ﺪﺣاو ( ﻪﻴﻬﺗ ﺪﻳدﺮﮔ . ﻲـﻨﺤﻨﻣ راﺪﻧﺎﺘـﺳا ﺎـﺑ ﻢﺳر ﻂـﺳﻮﺘﻣ بﺬـﺟ ﺖـﻈﻠﻏ دراﺪﻧﺎﺘـﺳا ﺮـﺑ يور رﻮـﺤﻣ يدﻮﻤﻋ ﺮﺑ ﺐﺴﺣ ﺖﻈﻠﻏ p53 ﺮﺑ يور رﻮـﺤﻣ ﻲـﻘﻓا ﻪـﺑ ﺖﺳد ﺪﻣآ . ﺲﭙﺳ ﻦﻳﺮﺘﻬﺑ ﻂﺧ زا نﺎـﻴﻣ طﺎـﻘﻧ ﺖـﺳد ﻪـﺑ ،هﺪﻣآ هﺪﻧارﺬﮔ ﺪﺷ ) 99989 / 0 = r ، ﻞﻜﺷ 1 .( ياﺮﺑ ﻦﻴﻴﻌﺗ ﺖﻈﻠﻏ ﻦﻴﺌﺗوﺮﭘ p53 اﺪﺘﺑا ﻦﻴﮕﻧﺎﻴﻣ بﺬـﺟ ياﺮﺑ ﺮﻫ ﻪﻧﻮﻤﻧ ﻲﻳﺎﺗود ﺖـﺳدﻪـﺑ هﺪـﻣآ و ﺎـﺑ هدﺎﻔﺘـﺳا زا ﻲﻨﺤﻨﻣ ﻂـﺧ دراﺪﻧﺎﺘـﺳا ) ﻞﻜـﺷ 1 ( ، ﺖـﻈﻠﻏ ﻦﻴﺌﺗوﺮـﭘ رد ﻪﻧﻮﻤﻧ نﻮﺧ داﺮﻓا ﻦﻴﻤﺨﺗ هدز ﺪﺷ . رد ﻞﻜﺷ 2 ناﺰﻴﻣ بﺬﺟ ﻪﻧﻮﻤﻧ يﺎﻫ ﻮﺗﺮﭘ رﺎﻛ و ﺪﻫﺎـﺷ رد 450 ﺮﺘﻣﻮﻧﺎﻧ ﻢﻴﺳﺮﺗ هﺪﺷ ﺖﺳا . راﺪﻘﻣ ﻂﺳﻮﺘﻣ بﺬـﺟ رد هوﺮﮔ رﺎﻛﻮﺗﺮﭘ 05237 / 0 ± 27664 / 0 و رد هوﺮﮔ ﺪﻫﺎـﺷ ﺮــﺑاﺮﺑ ﺎــﺑ 01755 / 0 ± 14594 / 0 ﺖــﺳدﻪــﺑ هﺪــﻣآ ﺖــﺳا ) ﻦﻴﮕﻧﺎﻴﻣ هاﺮﻤﻫﻪﺑ ﺖﺳد ﻪﺑيﺎﻫ SE ﺖـﺳاهﺪـﺷﻪﺋارا ( . ﻻﺎﺑ ندﻮﺑ ناﺰﻴﻣ بﺬﺟ ﻦﻳا ﻦﻴﺌﺗوﺮﭘ ياﺮﺑ هوﺮـﮔ رﺎـﻛﻮﺗﺮﭘ رد ﻪﺴﻳﺎﻘﻣ ﺎﺑ ﺪﻫﺎﺷ ﻼﻣﺎﻛ دﻮﻬﺸﻣ ﺖﺳا . ﺲﭙــﺳ لوﺪــﺟ ﻊــﻳزﻮﺗ ﻲــﻧاواﺮﻓ ﻦﻴﺌﺗوﺮــﭘ p53 ﻪــﻴﻬﺗ هﺪﻳدﺮﮔ و ﺎﺑ هدﺎﻔﺘﺳا زا نآ ﻲﻨﺤﻨﻣ ﻲـﻧاواﺮﻓ ﻦﻴﺌﺗوﺮـﭘ ﻪـﺑ ﺖﺳد ﺪﻣآ ) ﻞﻜﺷ 2 .( ياﺮﺑ ﻪﺴﻳﺎﻘﻣ ود هوﺮﮔ مزﻻ دﻮـﺑ ﺎـﺗ داﺪﻌﺗ داﺮﻓا رد ﺮﻫ هوﺮﮔ ﻪﺑ 100 هﺰﻳﻻﺎﻣﺮﻧ ﺪﻧﻮﺷ . ﺎﺑ ﻪﺟﻮـﺗ ﻪﺑ ﻞﻜـﺷ 2 ، ﺖﻈﻠﻏ ﻦﻴﺌﺗوﺮﭘ رد داﺮﻓا رﺎﻛﻮﺗﺮﭘ رد ﻪﺴﻳﺎﻘﻣ ﺎﺑ داﺮﻓا لﺮﺘﻨﻛ ﺮﺗﻻﺎﺑ هدﻮﺑ و ناﺰﻴﻣ ﻲﮔﺪﻨﻛاﺮﭘ نآ رد هوﺮﮔ رﺎﻛﻮﺗﺮﭘ ﺮﺘﺸﻴﺑ ﺖﺳا ) 0225 / 0 = p (. ﻪـﭽﻧﺎﻨﭼ ﻞﻜـﺷرد 2 هﺪﻳد ﻲﻣ ،دﻮﺷ رد هوﺮﮔ رﺎﻛﻮﺗﺮﭘ يداﺮﻓا ﺎﺑ ناﺰﻴﻣ ﺮﺗﻻﺎﺑ زا 30 ﻲـﺳﻲـﺳﺮﻫرد ﺪﺧاو ﻦﻴﺌﺗوﺮـﭘزا p53 ﺰـﻴﻧ هﺪـﻳد ﻲﻣ ﺪﻧﻮﺷ . رﺎـﻛﻪﻘﺑﺎـﺳﺐـﺴﺣﺮـﺑﻦﻴﺌﺗوﺮـﭘﻊـﻳزﻮﺗﻦﻳاﺮﺑهوﻼﻋ ردﻪـﻛﺖـﻓﺮﮔراﺮﻗﻪﻌﻟﺎﻄﻣدرﻮﻣﺰﻴﻧرﺎﻛﻮﺗﺮﭘداﺮﻓايﻮﺗﺮﭘ ﻞﻜﺷ 3 ﺖـﺳاهﺪـﺷ هدادنﺎﺸﻧ . ﺐﻳﺮـﺿزاهدﺎﻔﺘـﺳا ﺎـﺑ
ﻪﺴﻳﺎﻘﻣ زوﺮﺑ ﻦﻴﺌﺗوﺮﭘ p53 رد هوﺮﮔ نارﺎﻛﻮﺗﺮﭘ ... 39 ﻞﻜﺷ 2 . ﺮﻳدﺎﻘﻣماﺮﮔﻮﺘﺴﻴﻫ ﻦﻴﺌﺗوﺮﭘ P53 رﺎﻛﻮﺗﺮﭘهوﺮﮔودرد ﺪﻫﺎﺷو ﻞﻜﺷ 3 . داﺮﻓايﻮﺗﺮﭘرﺎﻛﻖﺑﺎﺳﺐﺴﺣﺮﺑﻦﻴﺌﺗوﺮﭘﺖﻈﻠﻏ راﺮـﻗ ﻲﺳرﺮﺑدرﻮﻣﺎﻫ نآ ﻦﻴﺑطﺎﺒﺗرانﻮﺳﺮﻴﭘﻲﮕﺘﺴﺒﻤﻫ يرﺎـﻣآﺰﻴﻟﺎـﻧآﻦـﻳاﺞﻳﺎﺘﻧﻪﻛﺖﻓﺮﮔ ) 27 / 0 = r ( زاﻲﻛﺎـﺣ رادﻲﻨﻌﻣﻲﮕﺘﺴﺒﻤﻫمﺪﻋ ﻘﺑﺎﺳوﻦﻴﺌﺗوﺮﭘ نﺎﻴﺑﻦﻴﺑ ﻪ رﺎـﻛ ﺖﺳانﺎﺒﻠﻃواد يﻮﺗﺮﭘ . نﺎـﻴﺑدرﻮـﻣ رد ﻲﻬﺑﺎـﺸﻣ ﻲﺳرﺮﺑ ﺰﻴﻧﻲﺳرﺮﺑﻦﻳاﻪﻛ ﺖﻓﺮﮔمﺎﺠﻧانﺎﺒﻠﻃواد ﻦﺳوﻦﻴﺌﺗوﺮﭘ نﺎﺸﻧارﻦﻴﺌﺗوﺮﭘنﺎﻴﺑناﺰﻴﻣوﺐﻠﻃوادﻦﺳ ﻦﻴﺑﻲﻃﺎﺒﺗرا داﺪﻧ ) ﺖﺳاهﺪﺸﻧهدادنﺎﺸﻧﺞﻳﺎﺘﻧ .( ﻪﻌﺟاﺮﻣﺎﺑ ﺎﻬﻨﺗ،هﺪﺷﺮﭘيﺎﻫمﺮﻓﻪﺑ 7 % ونارﺎﻛﻮﺗﺮﭘزا 5 / 6 % ﺪـﻧدﻮﺑيرﺎﮕﻴـﺳﺪﻫﺎـﺷداﺮﻓازا ) ﺎـﻫهداد ﺶﻳﺎـﻤﻧ ﻫداد هﺪﺸﻨ ﺪﻧا ( . طﺎﺒﺗرا ﻲﻨﻌﻣ ويرﺎﮕﻴـﺳداﺮﻓاﻦﻴﺑﺎﻣيراد ﻦﻴﺌﺗوﺮـﭘ نﺎـﻴﺑظﺎﺤﻟ ﻪﺑيرﺎﮕﻴﺳﺮﻴﻏ P53 ﺪـﺸﻧ هﺪـﻳد ) 1265 / 0 = p (. ﺚﺤﺑ و ﻪﺠﻴﺘﻧ يﺮﻴﮔ تﺎﻌﻳﺎﺿ ﻲـﺷﺎﻧ زا يﺎـﻫﻮﺗﺮﭘ ناﺰﻴﻧﻮـﻳ رد DNA لﻮﻠـﺳ ﺚﻋﺎﺑ زوﺮﺑ هﺮﺘﺴﮔ يا زا ﺦـﺳﺎﭘ ﺎـﻫ ﻞـﺜﻣ ﻒـﻗﻮﺗ ﻞﻜﻴـﺳ ،ﻲﻟﻮﻠﺳ ،زﻮﺘﭘﻮﭘآ گﺮﻣ يزﻮﺘﻴﻣ و ﻲﮔﺪﺷﺮﻴﭘ ﻲﻣ دﻮﺷ . ﻚﻳ يﺮﮔ ﻮﺗﺮﭘ ﻲﻣ ﺪﻧاﻮﺗ دوﺪﺣ 40 ﺖـﺴﻜﺷ ود ﻪﺘـﺷر يا رد DNA دﻮﺟﻮﺑ هدروآ و ﻂﻘﻓ ﻚﻳ ﺖـﺴﻜﺷ ود ﻪﺘـﺷر يا ﻢﻴﻣﺮــﺗ ،هﺪــﺸﻧ ﻲﻓﺎــﻛ ﺖــﺳا ﺎــﺗ گﺮــﻣ ﻲﻟﻮﻠــﺳ ار ﻢــﻗر ﺪــﻧز ) 16 و 17 .( رد ﻊــﻗاو ﻲــﻤﻛ ﺲــﭘ زا ﺶﺑﺎــﺗ يﺎــﻫﻮﺗﺮﭘ ،ناﺰﻴﻧﻮﻳ ﻦﻴﺌﺗوﺮﭘ p53 ﻪﺑ ناﻮﻨﻋ ﻚﻳ ) ﻆﻓﺎﺤﻣ مﻮـﻧژ ( ﻪـﺑ يﺎﻫﻲﻟاﻮﺗ ﻲﺻﺎﺧ زا DNA ) ﻲﺣاﻮـﻧرد ﻢﻴـﻈﻨﺗ هﺪـﻨﻨﻛ نژ ﺎﻫ ( ﻞﺼﺘﻣ ﻲﻣ دﻮﺷ . ﻦـﻳا طﺎـﻘﻧ ﻞﻣﺎـﺷ يﺎـﻫنﺎـﻜﻣ لﺮﺘﻨﻛ هﺪﻨﻨﻛ ﻞﻜﻴﺳ ،ﻲﻟﻮﻠﺳ ﻢﻴﻈﻨﺗ هﺪﻨﻨﻛ زﻮـﺘﭘﻮﭘآ و ﺰـﻴﻧ نﺎﻜﻣ ﻲﻳﺎﻫ ﺖﺳا ﻪﻛ ﻪﺑ رﻮﻃ ﻲﻔﻨﻣ دﻮـﺧ p53 ار ﻢﻴـﻈﻨﺗ ﻲﻣ ﺪﻨﻨﻛ . ﺎﭘ ﺦـﺳ p53 ﻦﻴـﻴﻌﺗ هﺪـﻨﻨﻛ ﺖـﺷﻮﻧﺮﺳ لﻮﻠـﺳ ﺐﻴﺳآ هﺪﻳد ﺖﺳا ﻪﻛ ﺎﻳ ﻢﻴﻣﺮﺗ DNA و ﺎﻳ گﺮﻣ يزﻮﺘﻴﻣ ار ﻲﻃ ﺪﻨﻛ . ﻦﻳاﺮﺑﺎﻨﺑ ﻦﻴﺌﺗوﺮﭘ p53 ﻚﻳ نژ زﺎﺑ هﺪـﻧراد ي يرﻮﻣﻮﺗ ﺖﺳا و ﺎﺗ ﻲﻧﺎﻣز ﻪﻛ لﻮﻠﺳ ﻲﻣ ﺪـﻧاﻮﺗ p53 ﺪـﻴﻟﻮﺗ ،ﺪﻨﻛ ﻊﻧﺎﻣ ﻪﻌﺳﻮﺗ ي نﺎﻃﺮﺳ ﻲﻣ ددﺮﮔ . راﺪﻘﻣ p53 رد لﻮﻠﺳ يﺎﻫ ﻲﻌﻴﺒﻃ ﻢﻛ ﺖﺳا و ﻪﺑ رﻮـﻃ
ﺮﺘﻛد ﻼﻬﺷ يﺪﻤﺤﻣ نارﺎﻜﻤﻫ و 40 ﻪﺘﺳﻮﻴﭘ ﺪـﻴﻟﻮﺗ و ﺐـﻳﺮﺨﺗ ﻲـﻣ دﻮـﺷ . رد ترﻮـﺻ ﺐﻴـﺳآ DNA ، ﺐﻳﺮﺨﺗ ﻦﻴﺌﺗوﺮﭘ p53 ﻒﻗﻮﺘﻣ هﺪﺷ و راﺪﻘﻣ p53 رد لﻮﻠﺳ ﻪﺑ تﺪﺷ ﺶﻳاﺰﻓا اﺪﻴﭘ ﻲﻣ ﺪـﻨﻛ . ﻦﻴﺌﺗوﺮـﭘ p53 ﻪﺑ نژ ﻲﻳﺎﻫ ﻪﻛ زﺎﺑ يزﺎـﺳ DNA ار ﻢﻴـﻈﻨﺗ ﻲـﻣ ،ﺪـﻨﻨﻛ ﻞﺼﺘﻣ هﺪـﺷ و گﺮـﻣ ﻪـﻣﺎﻧﺮﺑ يﺰـﻳر هﺪـﺷ ي لﻮﻠـﺳ ار لﺮﺘﻨﻛ ﻲﻣ ﺪﻨﻛ . ﺢﻄﺳ دﺎـﻳز ﻦﻴﺌﺗوﺮـﭘ p53 رد ﻪـﻠﺣﺮﻣ ي ،لوا ﻪﺧﺮﭼ ي ﻲﻟﻮﻠﺳ ار ﻒﻗﻮﺘﻣ ﻲﻣ ﺪﻨﻛ ﺎﺗ ﺖﺻﺮﻓ ياﺮـﺑ يزﺎﺳزﺎﺑ DNA ﺐﻳﺮﺨﺗ هﺪﺷ ﻪﺑ دﻮﺟو ﺪﻳآ . ﺮﮔا ﺐـﻳﺮﺨﺗ رﺎﻴﺴﺑ ﺪﻳﺪـﺷ ،ﺪـﺷﺎﺑ لﻮﻠـﺳ گﺮـﻣ ﻪـﻣﺎﻧﺮﺑ يﺰـﻳر هﺪـﺷ و » ﻲﺸﻛدﻮﺧ لﻮﻠﺳ « ار زﺎـﻏآ ﻲـﻣ ﺪـﻨﻛ . ﻲـﻟو زا ﻲـﻓﺮﻃ تﺎﻌﻟﺎﻄﻣ نﺎﺸﻧ هداد ﺪﻧا ﻪﻛ رد ﻞﻗاﺪﺣ 50 ﺪﺻرد زا مﺎـﻤﺗ نﺎﻃﺮﺳ ،ﺎﻫ p53 دﻮﺧ رﺎﭼد ﺮﻴﻴﻐﺗ هﺪﻳدﺮﮔ ﺖـﺳا ) 11 -9 ( . تﺎﻘﻴﻘﺤﺗ ﻪﻠـﺻﺎﺣ ﺮـﺑ يور ﻦـﻳا نژ ﻲـﺸﺣو p53 و ﺎـﻳ ﺖﻟﺎﺣ ﺶﻬﺟ ﻪـﺘﻓﺎﻳ ﻦـﻳا ،نژ ﻲﻛﺎـﺣ زا ﻦـﻳا ﺖـﺳا ﻪـﻛ ﺖﻴﺳﺎﺴﺣ يﻮﺗﺮﭘ ﻪﺑ رﻮﻃ ﻂﺳﻮﺘﻣ ﺎﺑ دﻮﺒﻤﻛ ﻦﻴﺌﺗوﺮﭘ p53 ﺶﻫﺎﻛ ﻲﻣ ﺪﺑﺎﻳ ) 18 .( رد ﺶﻫوﮋﭘ ﺮﺿﺎﺣ ﺮﺑ يور داﺮﻓا ﻂﺒﺗﺮﻣ ﺎﺑ ،ﻮﺗﺮﭘ ناﺰـﻴﻣ p53 رد مﺮﺳ نﻮﺧ ﻲﻄﻴﺤﻣ ﺎﺑ ﻚﻳ هوﺮﮔ ﺪﻫﺎـﺷ ﻪـﺴﻳﺎﻘﻣ هﺪﺷ ﺖﺳا . ﺞﻳﺎﺘﻧ ﻦﻳا هزاﺪﻧا يﺮﻴﮔ ﻲﻛﺎﺣ زا ﺮﺗﻻﺎـﺑ ندﻮـﺑ ﻦﻳا ﻦﻴﺌﺗوﺮﭘ رد يﺎﻤﺳﻼﭘ هوﺮـﮔ رﺎـﻛﻮﺗﺮﭘ ﺖـﺳا . ﻦـﻜﻤﻣ ﺖﺳا نﺎﻴﺑ يﻻﺎﺑ ﻦﻴﺌﺗوﺮﭘ سﺎﻜﻌﻧا رﻮﻀﺣ مواﺪﻣ ﻮـﺗﺮﭘ رد ﻂﻴﺤﻣ رﺎﻛ و سﺮﺘﺳا ﻲﺷﺎﻧ زا نآ ﻪﺑ لﻮﻠﺳ ﺪﺷﺎﺑ ﻪـﻛ ﻪـﺑ ﺶﻳاﺰﻓا p53 ﻪﺑ رﻮﻈﻨﻣ ترﺎﻈﻧ ﻪﺑ ﺐﻴـﺳآ يﺎـﻫ DNA ﺮﺠﻨﻣ هﺪﺷ ﺖﺳا . رد ﻊﻗاو ﻦﻳا ﺢﻄﺳ يﻻﺎﺑ نﺎﻴﺑ نژ يﺎﻫ يﺮﻴﻤﻌﺗ DNA رد رﺎﻛﻮﺗﺮﭘ ﻲﻣ ﺪﻧاﻮﺗ ﻲﺷﺎﻧ زا لﺎﻌﻓ نﺪﺷ ﺮﻴﺴﻣ يﺎﻫ ﺮـﻴﻤﻌﺗ DNA و رد ﺦـﺳﺎﭘ ﻪـﺑ تاﺮـﺛا هﺎـﺗﻮﻛ تﺪﻣ و ﺰﻴﻧ ﺪﻨﻠﺑ تﺪﻣ يﺎﻫزود ﻦﻴﻳﺎﭘ ﻮﺗﺮﭘ ﺪـﺷﺎﺑ ؛ ﺪـﻨﭼﺮﻫ ﻪﻛ ﻴﻣ ناﺰ نﺎﻴﺑ ﻦﻳا نژ رد ﻲﺧﺮﺑ زا داﺮﻓا رﺎﻛﻮﺗﺮﭘ ) داﺮـﻓا هرﺎﻤﺷ 3 ، 19 ، 22 ، 26 و 27 ( رﺎﻴﺴﺑ ﻻﺎﺑ هدﻮـﺑ و مﺰﻠﺘـﺴﻣ ﻲﺳرﺮﺑ يﺎﻫ ﺮﺘﺸﻴﺑ ﻲﻣ ﺪﺷﺎﺑ . رد ﻲــﺷراﺰﮔ ﻪــﻛ Rössner و ﺶﻧارﺎــﻜﻤﻫ ) 19 ( ﻪــﺋارا هدﺮﻛ ،ﺪﻧا ناﺰﻴﻣ نﺎﻴﺑ ﻦﻳا ﻦﻴﺌﺗوﺮﭘ ار رد يﺎﻤـﺳﻼﭘ هوﺮـﮔ رﺎﻛﻮﺗﺮﭘ ﻞﺑﺎﻗ ﻪﺴﻳﺎﻘﻣ ﺎﺑ هوﺮﮔ ﺪﻫﺎﺷ ﻪـﺘﻓﺎﻳ ﺪـﻧدﻮﺑ ﻪـﻛ ﺎـﺑ ﺞﻳﺎﺘﻧ ﻪﺋارا هﺪﺷ رد ﻦﻳا ﻖﻴﻘﺤﺗ ﺖﻘﺑﺎﻄﻣ ؛دراﺪﻧ ﺪﻨﭼﺮﻫ ﻪﻛ نآ ﺎﻫ ناﺰﻴﻣ نآ ار رد ﺖﻴﺳﻮﻔﻨﻟ نارﺎﻛﻮﺗﺮﭘ ﺮﺘﺸﻴﺑ شراﺰـﮔ ﺪﻧدﺮﻛ . تﺎﻘﻴﻘﺤﺗ يدﺪﻌﺘﻣ نﺎﺸﻧ ﻲﻣ ﺪـﻨﻫد ﻪـﻛ ﻦﻴﺌﺗوﺮـﭘ p53 ،ﻲﻌﻴﺒﻃ نوﺪﺑ ﻲﺸﻬﺟ رد DNA ﻦـﻜﻤﻣ ﺖـﺳا رد ﺎﻫرﻮﻣﻮﺗ ﻪﺘﺷﺎﺒﻧا ﺪﻧﻮﺷ . هوﻼﻋ ﺮﺑ ،ﻦﻳا ﺎﺑ ﺶﻧاد ،ﺎﻣ رد هداﻮﻧﺎﺧ يﺎﻫ ﺪﻌﺘـﺴﻣ ﻪـﺑ ،نﺎﻃﺮﺳ ﻦﻴﺌﺗوﺮﭘ p53 ﻦﻜﻤﻣ ﺖﺳا رد ﺖﻓﺎﺑ يﺎـﻫ ﺮـﻴﻏ ﻚﻴﺘﺳﻼﭘﻮﺌﻧ ﺎﻳ رد ﺖﻓﺎﺑ يﺎﻫ ﺎﺑ لﻮﻠﺳ يﺎﻫ ﻲـﻌﻴﺒﻃ ﺰـﻴﻧ ﻊﻤﺠﺗ ﺪﻨﺑﺎﻳ ) 20 و 21 .( ﺮﻫ ود ﻞﻣاﻮﻋ ﻲﻜﻴﺘﻧژ و ﻴﻄﻴﺤﻣ ﻲﻤ ﺪﻨﻧاﻮﺗ دﺮـﻜﻠﻤﻋ p53 ار ﻞﻳﺪــﻌﺗ ﺪــﻨﻳﺎﻤﻧ . ﺢــﺿاو ﺖــﺳا ﻪــﻛ تاﺮــﻴﻴﻐﺗ رد نﺎــﻴﺑ ﻦﻴﺌﺗوﺮﭘ p53 ﺮﺠﻨﻣ ﻪﺑ تﻻﻼﺘﺧا رد ﻢﻴﻈﻨﺗ ﻪﺧﺮﭼ ﻲﻟﻮﻠﺳ ﻲﻣ ددﺮﮔ ) 22 .( ﻦﻴﺌﺗوﺮﭘ p53 لﺎﻣﺮﻧ ﻲﻣ ﺪﻧاﻮﺗ لﻮﻠﺳ ﺎﻫ ار رد يﺎﻬﺘﻧا زﺎﻓ G1 ﻪﺧﺮﭼ ﻲﻟﻮﻠـﺳ ﻲـﻨﻌﻳ ﺖـﺳرد ﻞـﺒﻗ زا ﺰﺘﻨﺳ DNA ﻒﻗﻮﺘﻣ ،دزﺎﺳ رد ﻲﻟﺎﺣ ﻪـﻛ لﺎﻜـﺷا ﺶـﻬﺟ ﻪﺘﻓﺎﻳ ﻦﻳا ﻦﻴﺌﺗوﺮﭘ ﻖﻓﻮﻣ ﻪﺑ ﻒﻗﻮﺗ ﻪﺧﺮﭼ ﻲﻟﻮﻠﺳ هﺪـﺸﻧ و ﺮﻴﺜﻜﺗ ﻲﻟﻮﻠﺳ ﻪﻣادا ﻲﻣ ﺪﺑﺎﻳ . يرﺎﻴﺴﺑ زا تﺎﻌﻟﺎﻄﻣ رد درﻮﻣ نﺎﻴﺑ p53 ﺮﺑ ﺖﻴﻤﻫا ﻪـﻤﻴﻧ ﺮـﻤﻋ ﻲﻧﻻﻮـﻃ p53 ﺶـﻬﺟ ﻪﺘﻓﺎﻳ ﺪﻴﻛﺎﺗ ﻲﻣ ﺪﻨﻨﻛ ﻪـﻛ ﺮـﺠﻨﻣ ﻪـﺑ ﻊـﻤﺠﺗ ﻦﻴﺌﺗوﺮـﭘ رد ﺖﻓﺎﺑ يﺎﻫ ﻚﻴﺘﺳﻼﭘﻮﺌﻧ ) 23 ( ﻲﻣ دﻮﺷ . ﻲﻟو نﺎـﻴﺑ رﺎﻴـﺴﺑ يﻻﺎـﺑ ﻦﻴﺌﺗوﺮـﭘ p53 ﻲــﺸﺣو رد ﻪـﻧﻮﻤﻧ يﺎـﻫ يرﻮــﻣﻮﺗ ،نﺎﺴﻧا و ﺰﻴﻧ رد تﺎﻌﻳﺎﺿ شﻮﺧ ﻢﻴﺧ نوﺪﺑ ﺶـﻬﺟ p53 ﺰﻴﻧ هﺪﻫﺎﺸﻣ هﺪﺷ ﺖﺳا ) 20 .( ﻦﻳاﺮﺑﺎﻨﺑ نﺎﻴﺑ يﻻﺎـﺑ ﻦﻴﺌﺗوﺮـﭘ p53 ، ﻦـﻜﻤﻣ ﺖـﺳا ًﺎﻣوﺰﻟ ﻪﺑ ﺖﻠﻋ ﺶﻬﺟ رد ﻲﺣاﻮـﻧ ﺪـﻛ هﺪـﻨﻨﻛ نوﺰـﮔا نژ ﺪﺷﺎﺒﻧ ) 24 .( زا ﻪﻛ ﻲﻳﺎﺠﻧآ ﺶﻬﺟ رد نژ p53 ﻚﻳ ﻞـﻣﺎﻋ ﺶﻴﭘ ﻲﻬﮔآ هﺪﻨﻫد ﻢﻬﻣ هدﻮﺑ ﻪـﻛ ﺪـﻧاﻮﺗ ﻲـﻣ ﺮـﺠﻨﻣ ﻪـﺑ ﻊﻤﺠﺗ ﻦﻳا ﻦﻴﺌﺗوﺮﭘ رد ﻪﺘﺴﻫ يﺎﻫ لﻮﻠﺳ يﺎﻫ ﻲﻧﺎﻃﺮـﺳ ددﺮﮔ ) 27 -25 ( ، ﻲـﺳرﺮﺑ PCR ﻦـﻳا نژ و هزاﺪـﻧا يﺮـﻴﮔ ،زﻮﺘﭘﻮﭘآ تﺎﻋﻼﻃا ﻊﻣﺎﺟ يﺮﺗ رد درﻮﻣ ﻢﺴﻴﻧﺎﻜﻣ دﺮـﻜﻠﻤﻋ ﻦﻳا ﻦﻴﺌﺗوﺮﭘ رد درﻮﻣ ﻦﻳا هوﺮﮔ يرﺎـﻛ رد رﺎـﻴﺘﺧا ﺪـﻫاﻮﺧ ﺖﺷاﺬﮔ ) 28 .( ﺞﺑﺎﺘﻧ ﻞﺻﺎﺣ زا ﺰﻴﻟﺎﻧآ يرﺎﻣآ نﺎﺸﻧ هداد ﻪﻛ ناﺰﻴﻣ نﺎـﻴﺑ ﻦﻳا ﻦﻴﺌﺗوﺮﭘ ﻪﺑ ﻪﻘﺑﺎﺳ رﺎﻛ داﺮﻓا و ﺰﻴﻧ ﻪﺑ فﺮﺼﻣ تﺎﻴﻧﺎﺧد ﻲﮕﺘــﺴﺑ دراﺪــﻧ ) p>0.05 .( ﻦــﻳا ﺞﻳﺎــﺘﻧ رد ﻖﺑﺎــﻄﺗ ﺎــﺑ تاﺪﻫﺎﺸﻣ Rössner و ﺶﻧارﺎـﻜﻤﻫ ) 29 ( و ﺰـﻴﻧ Garaj-Vrhovac و شرﺎﻜﻤﻫ ) 30 ( ﻲﻣ ﺪﺷﺎﺑ . هزوﺮﻣا دروآﺮـﺑ تاﺮـﻄﺧ ﻲـﺷﺎﻧ زا زود يﺎـﻫ ﻦﻴﻳﺎـﭘ رد ،ﻂﻴﺤﻣ ﻲﺸﻟﺎﭼ ﻲﻣﻮﻤﻋ بﻮﺴﺤﻣ ﻲـﻣ دﻮـﺷ . رد ود ﻪـﻫد ﺮﻴﺧا ﻢﻏر ﻲﻠﻋ ﺶﻳاﺰﻓا هدﺎﻔﺘﺳا زا ﻮﺗﺮﭘ رد ،ﻲﻜﺷﺰﭘ ﻮـﺗﺮﭘ يﺮﻴﮔ يﺎﻫ ﻲﻠﻐﺷ ﺶﻫﺎﻛ ﻪﺘﻓﺎﻳ ﺖﺳا . ﻲﻜﻳ زا ﻞﻳﻻد ﻦﻳا ﺮﻣا يوﺮـﻴﭘ ﺮﺘـﺸﻴﺑ نارﺎـﻛﻮﺗﺮﭘ زا تارﺮـﻘﻣ و شور يﺎـﻫ ﻲﻨﻤﻳا نوﺪﻣ ﺖﺳا ﻪﺑ يﻮﺤﻧ ﻪﻛ يﺮﻴﮔﻮـﺗﺮﭘ نﺎـﻨﻛرﺎﻛ رد هدوﺪﺤﻣ زود زﺎﺠﻣ راﺮﻗ ﻪﺘﻓﺮﮔ ؛ﺖﺳا هدوﺪﺤﻣ يا ﻪﻛ ﻪـﺑ نآ هدوﺪﺤﻣ ﻲﺑ ﺮﻄﺧ قﻼﻃا ﻲﻣ ددﺮـﮔ . ﻲـﻟو رد ﻖـﻴﻘﺤﺗ ﺮﺿﺎﺣ ناﺰﻴﻣ نﺎﻴﺑ ﻦﻴﺌﺗوﺮﭘ p53 رد ﻦﻴﺑ نارﺎﻛﻮﺗﺮﭘ ﺮﺗﻻﺎﺑ زا داﺮﻓا ﺪﻫﺎﺷ هﺪﻫﺎﺸﻣ هﺪﺷ ﺖـﺳا . رد ﻲﻟﺎـﺣ ﻪـﻛ ﺞﻳﺎـﺘﻧ
ﻪﺴﻳﺎﻘﻣ زوﺮﺑ ﻦﻴﺌﺗوﺮﭘ p53 رد هوﺮﮔ نارﺎﻛﻮﺗﺮﭘ ... 41 ﺶﻳﺎﭘ ﻦﻳا نارﺎﻛﻮﺗﺮﭘ ﻲﻛﺎﺣ زا ﺖﻓﺎﻳرد يزود رد هدوﺪﺤﻣ زود زﺎﺠﻣ ﻪﻧﻻﺎﺳ ياﺮﺑ نارﺎﻛﻮﺗﺮﭘ هدﻮﺑ ﺖﺳا . ﻦﻳا ﺞﻳﺎﺘﻧ نﺎﺸﻧ ﻲﻣ ﺪﻫد ﻪﻛ يﺮﺘﻤﻳزود يﺎـﻫ ﻲـﻜﻳﺰﻴﻓ ﺪﻳﺎــﺷ تﺎــﻋﻼﻃا ﻲﻠﻣﺎــﻛ ار رد درﻮــﻣ ﻊــﻳزﻮﺗ و ناﺰــﻴﻣ يﺮﻴﮔﻮﺗﺮﭘ داﺮﻓا نﺎﺸﻧ ﻲﻤﻧ ﺪﻫد . رد ﻪﻛ ﻲﻟﺎﺣ ﺎﺑ ﻪـﺟﻮﺗ ﻪـﺑ تﺎــﻴﺑﺮﺠﺗ ﻞــﺻﺎﺣ زا ثداﻮــﺣ ،يﻮــﺗﺮﭘ ﺺﺧﺎــﺷ يﺎــﻫ ﻲﻜﻳژﻮﻟﻮﻴﺑ ﻪﺑ رﻮﻈﻨﻣ ﺖﺳد ﻪﺑ ندروآ تﺎﻋﻼﻃا طﻮﺑﺮﻣ ﻪﺑ ﻊﻳزﻮﺗ ناﺰﻴﻣو يﺮﻴﮔﻮﺗﺮﭘ رﺎﻴﺴﺑ ﻲﺗﺎﻴﺣ ﺖﺳا . ﻪﺑ هوﻼﻋ زا ﻪﻛ ﻲﻳﺎﺠﻧآ داﺮﻓا ياراد ﺖﻴﺳﺎﺴﺣ يﻮـﺗﺮﭘ ﻲﺗوﺎﻔﺘﻣ ،ﺪﻨﺘﺴﻫ ﺺﺧﺎﺷ يﺎﻫ ﻲﻜﻳژﻮـﻟﻮﻴﺑ ياراد ﺖـﻳﺰﻣ يﺮﺘﺸﻴﺑ ﺪﻨﺘﺴﻫ . ،ﻦﻳاﺮﺑﺎﻨﺑ ياﺮﺑ نﺎﻨﻴﻤﻃا زا دﻮﺟو ﺮﺜﻛاﺪﺣ ﻲﻨﻤﻳا ،ﻲﻠﻐﺷ ﺶﻳﺎﭘ ﻲﻜﻳژﻮـﻟﻮﻴﺑ نارﺎـﻛﻮﺗﺮﭘ ياراد شزرا يرﺎﻴﺴﺑ ﺖﺳا . ﻦﻴﻨﭼ شور ﻲﻳﺎـﻫ ﻲـﻣ ﺪـﻧاﻮﺗ ﻪـﺑ ناﻮـﻨﻋ ﺮﺘﻤﻳزود ﻲﻠﺧاد ياﺮـﺑ ﺶﻳﺎـﭘ دﺮـﻓ و عﻼـﻃا زا ﺶﻳاﺰـﻓا ﻲﻟﺎﻤﺘﺣا ﻞﻣاﻮﻋ ﻚﻴﺴﻛﻮﻧژ و ًﻻﺎﻤﺘﺣا ﺮﻄﺧ نﺎﻃﺮﺳ ﻲـﻳاز رد ﺮﻈﻧ ﻪﺘﻓﺮﮔ دﻮﺷ . ﺮﻜﺸﺗوﺮﻳﺪﻘﺗ ﻦﻳﺪﺑ ﻪﻠﻴﺳو زا قوﺪﻨـﺻ ﺖـﻳﺎﻤﺣ زا ناﺮﮕـﺸﻫوﮋﭘ ﻪـﻛ ﻲﺸﺨﺑ زا ﻪﺟدﻮﺑ ﻦﻳا رﺎﻛ ﻲﺗﺎﻘﻴﻘﺤﺗ ار ﻦﻴﻣﺎﺗ هدﻮﻤﻧ ﺖـﺳا ﺮﻜﺸﺗ ﻲﻣ دﻮﺷ . ﻦﻴﻨﭽﻤﻫ زا يﺎﻗآ ﺮﺘﻛد ﻲﻫﺎﺷﺮﻴﻣ و ﺪـﺣاو ﺖﻇﺎﻔﺣ رد ﺮﺑاﺮﺑ ﻪﻌﺷا ﻪـﻛ هﺎﮕـﺸﻳﺎﻣزآ يﺎـﻫ دﻮـﺧ ار رد رﺎﻴﺘﺧا ﻦﻳا هوﺮﮔ ﻲـﺸﻫوﮋﭘ راﺮـﻗ هداد ،ﺪـﻧا يراﺰﮕـﺳﺎﭙﺳ ﻲﻣ ددﺮﮔ . ﻊﺑﺎﻨﻣ
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Original Article
Comparison of p53 expression between occupationally exposed to
ionizing radiation and control group
*Shahla Mohammadi, Ph.D. Assistant Professor of Molecular Biophysics, Nuclear Science Research School, Nuclear Science & Technology Research Institute, Tehran, Iran (*Corresponding author). smohammadi@aeoi.org.ir
Mohammad Reza Gharaati, MSc. PhD candidate of Biochemistry, Faculty of Science, Tarbiat Modares University, Tehran, Iran. gharaati@modares.ac.ir
Kobra Ezaki Khan Alang, MSc. PhD candidate of Molecular Medicine, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran. ezakikhan@yahoo.com
Rasa Rajaie, MSc. Radiation Applications Research School, Nuclear Science & Technology Research Institute, Tehran, Iran. rrajaie@aeoi.org.ir
Abstract
Background: Various stresses such as ionizing radiation can increase cellular damage, especially to
nuclear DNA. To protect cellular damages, normal regulatory genes (such as Tp53 tumor suppressor) become activated. Accordingly, in this study, the p53 gene and its expression among employees occupationally exposed to low doses of ionizing radiation were compared with a selected control group.
Methods: Using an ELISA kit, the amount of p53 protein in the blood serum of case and control
groups was measured. Statistical analysis was performed using student t-test procedure. As a case group, 42 healthy individuals (with a mean age of 37.8 ± 1.3 years) occupationally dealing with different kind of radiation sources in Atomic Energy Organization of Iran were chosen. As a control group, 16 healthy unexposed volunteers (with a mean age of 34.5 ± 2.0 years) who were matched for age, sex and smoking habits were selected. A 2 ml aliquot of heparinized peripheral blood was collected from each case.
Results: The results of this research indicate the higher levels of p53 expression in occupationally
radiation group. Moreover, the work history or smoking had no effects on p53 expression.
Conclusion: Although the absorbed doses were below the permissible limits, this study confirms the
role of low-level chronic exposure in increasing p53 expression among occupationally radiation workers. These results confirm that monitoring of radiation workers should not only be solely based on physical dosimetry, but also on the biological indicators, which have the advantage of measuring the individual radiation damage.
