Ş갬릤䬬§산§䬬산§산ꮠ산§릤§산ö
ã§Ū릤䬬ꮠ갬§산䨸 ö§릤ú산 §ĺ§èĢnðnĨĖðĮĮĐĀĐĨ§ Ŷ牨산ꮠ§산릤䬬䬬§ĺ§산öꮠ䬬산Ř갬牨산ꮠö牨§
INHIBITION OF LIPID PEROXIDATION INDUCED BY ULTRAVIOLET RADIATION
BY CRUDE PHLOROTANNIS ISOLATED
FROM BROWN ALGAE
Sargassum hystrix
v. buxifolium
C. Agardh
Agnes Nora Iska Harnita1,*, Ign. Edi Santosa2, Sudibyo Martono3, Sudarsono3, Sitarina Widyarini4, and Frans J.M. Harren5 1
Faculty of Pharmacy, Sanata Dharma University, Yogyakarta, Indonesia
2
Dept of Physics Education, Sanata Dharma University, Yogyakarta, Indonesia
3
Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
4
Faculty of Veterinary Science, Universitas Gadjah Mada, Yogyakarta, Indonesia
5
Life Science Trace Gas Facility, Molecular and Laser Physics, Radboud University, Nijmegen, Netherlands§
Received November 9, 2012; Accepted March 22, 2013
§
ABSTRACT
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This study examines the antioxidant activity of crude phlorotannins from the brown algae Sargassum hystrix v. buxifolium (Chauvin) J. Agardh, through the inhibition of a lipid peroxidation reaction that is induced by the UV radiation. The antioxidant activity during the UV exposure was investigated using the laser-based photoacoustic method for the detection of the ethylene as indicator for lipid peroxidation. This involves an experiment that isolated crude phlorotannins from the ethyl acetate fraction of the Sargassum hystrix methanol extract, hereafter referred to as PFSH. It results in the antioxidant activity as a potent lipid peroxidation inhibitor. Statistically, such antioxidant activity is not significantly different than the commercial antioxidant, which is vitamin C (p > 0.05). The amount of the total phlorotannins, using the Folin-Ciocalteu method, was measured to be approximately 0.13% w/w. In addition, it is found that PFSH contains phlorotannins with low molecular weight (MW) (<1000 Da).
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Keywordsĺ§phlorotannins; Sargassum hystrix; lipid peroxidation; photoacoustic; Folin Ciocalteu method§
ABSTRAK
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Penelitian ini bertujuan untuk menguji aktivitas antioksidan fraksi phlorotannin alga coklat Sargassum hystrix v.buxifolium C. Agardh melalui penghambatan reaksi lipid peroksidasi akibat radiasi UV. Aktivitas antioksidan selama pemaparan UV diuji dengan metode fotoakustik berbasis laser untuk mendeteksi etilen sebagai indikator lipid peroksidasi. Penelitian ini menggunakan fraksi phlorotannin, yaitu fraksi etil asetat ekstrak metanol Sargassum hystrix, yang selanjutnya dirujuk sebagai FPSH. Hasil penelitian menunjukkan bahwa FPSH adalah inhibitor lipid peroksidasi yang potent. Secara statistik, aktivitas antioksidan FPSH tersebut tidak berbeda secara signifikan dibandingkan antioksidan komersial, yaitu vitamin C (p > 0,05). Kadar phlorotannin total ditentukan dengan metode Folin-Ciocalteu yaitu sebesar sekitar 0,13% b/b. Selain itu, penelitian ini menemukan FPSH mengandung phlorotannin dengan berat molekul (BM) rendah (<1000 Da).
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Kata Kunciĺ§phlorotannins; Sargassum hystrix; lipid peroksidasi; fotoakustik; metode Folin Ciocalteu§ §
INTRODUCTION
릤§릤산ꮠ릤§ 䀸갬릤§䬬릤ꮠ릤䬬§×鯈⟴ݧꮠ䬬§갬릤릤산릤§ 산䬬§산§䬬릤ɬ䨸릤릤§ § 릤§䨸산ꮠ릤§×쀔ݧ릤 䬬䨸릤§ 산§ 䨸릤䬬§ ꮠ산ꮠ릤§䬬릤䬬䬬§Ć㮴ö§⟴牨릤§鯈⟴î§ꮠ䨸ꮠ갬§ 䬬䨸릤 ꮠ릤§산ꮠ䬬î§ 䀸 䀸§산ꮠ산䬬î§산§ 䀸갬릤§ 릤 ꮠ릤î§산릤§ꮠ牨산§牨릤ꮠ산䬬§ꮠ§ 릤§ꮠꮠꮠ산ꮠ§ § 릤§ ꮠꮠ§릤 ꮠ산ꮠö§ ꮠ䬬§牨산ꮠ䀸§䨸䬬§ꮠ§ꮠ牨릤牨산릤䬬î§ 䘐 릤릤§ 릤§릤§ §䨸䬬산䨸산릤§ 산䀸§산ꮠ䬬§ꮠ䬬§릤산ꮠ릤䀸§ ꮠ갬 ö§ ꮠ䬬§릤산䬬§§ 릤§ 릤䬬䨸ꮠ§ § 릤䨸산§
Ş갬릤䬬§산§䬬산§산ꮠ산§릤§산ö
EXPERIMENTAL SECTION
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Collection of plant material
릤§䘐§산갬산릤§⟴산갬산䬬䬬䨸牨§ 䀸䬬ꮠ §ö§䨸 ꮠ ꮠ䨸牨§
Preparation of extracts
릤§䨸릤§ 산ꮠ䬬§䘐릤릤§릤 산릤§ 牨§ 릤§
Determination of total phenolic
Ş갬릤䬬§산§䬬산§산ꮠ산§릤§산ö
Identification and characterization of low MW (Molecular Weight) phlorotannins
ާꮠ§×ĀöĜ§갬ݧ §⟴§䘐산䬬§ꮠ䬬䬬릤§ꮠ§Ė§牨§
Inhibitory activity of lipid peroxidation of PFSH using the laser-based photoacoustic method
Ťꮠ릤산牨 §䘐산䬬§䬬牨릤산릤§§산§갬산䬬䬬§산릤§×Ė§牨§ §
RESULT AND DISCUSSION§ §
Determination of Total Phenolic§ §
kjellamanianum§䘐 ꮠ §ꮠ䬬§ꮠꮠ산릤§산䬬§산§산ꮠ ꮠ산§n㮴ö§
Ş갬릤䬬§산§䬬산§산ꮠ산§릤§산ö
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Fig 2.§ 릤§산릤§牨릤䨸산§ꮠ䬬§×સè㮴èݧ릤릤§산牨ú§ĜĀnĀĀ§산갬릤§䬬릤䨸牨§ §⟴ §
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Fig 3.§ 릤§산릤§牨릤䨸산§ꮠ䬬§×સè㮴èݧ릤릤§산牨ú§ĆĀĀĆĀĀĀ§산갬릤§䬬릤䨸牨§ §⟴ §
Identification and Characterization Low MW (Molecular Weight) Phlorotannins§
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§ ꮠ䬬§䬬䨸䀸î§ 릤§ 䀸 릤산릤§릤 ꮠɬ䨸릤§ §ŪðŶ⟴ð સ⟴§䘐산䬬§䨸䬬릤§§산산䀸릤§ 릤§ 릤牨ꮠ산§ ꮠ갬릤ꮠ䬬§ § 릤§ ɬ䨸산ꮠ䀸§§산§ 릤§䬬산산ꮠ산ꮠ§ §䘐§산갬산릤ö§ 릤§䨸ꮠ갬§ §산§䬬릤산산ꮠ§릤 ꮠɬ䨸릤§산§산§ðꮠ릤§ 䬬릤䬬ꮠ§ 릤릤ꮠ§ 릤 갬䀸§ ꮠ䬬§ 䘐§ 산䬬§ 䀸 릤산릤§ 릤 ꮠɬ䨸릤§ nĀ㮴ö§ ⟴릤릤산§ 䬬䨸ꮠ릤䬬§ 산릤§ ꮠ릤ꮠ ꮠ릤§ 산§ 릤§릤 䀸§산릤산릤§ 산ꮠ§ § 䬬릤릤산§ 䬬릤ꮠ릤䬬§ §䘐§산갬산릤§산ꮠ§ 산ꮠ䬬§ 牨§ 릤§ 갬䨸ꮠ§×牨牨릤ݧ䨸ꮠ§ 산ꮠ䬬§×ꮠ갬牨릤Ýö§ 릤§릤 산牨릤䬬§ § 산ꮠ䬬§ꮠ갬牨릤§산릤§릤§ ×ꮠ牨릤Ýî§ 䨸 䨸릤§ ާ ×릤산牨릤Ýî§ ꮠ릤§ × 릤 산牨릤Ýî§산§ĮöĮ§Ñðꮠ릤§× 릤 산牨릤ݧĆnðĆĐînĆðnn㮴ö§ ꮠ䬬§䬬䨸䀸§䨸䬬릤§ 릤§산릤§牨릤䨸산§ꮠ§×સè㮴èÝö§ 릤§牨산䬬䬬§䬬릤산§ §산ꮠ䨸䬬§牨릤䬬§ 산§산릤§ 릤䬬릤§ꮠ§⟴§ 산䬬§릤릤§산ꮠ릤§ðꮠ릤§ 牨§ 릤§Ūð
Ş갬릤䬬§산§䬬산§산ꮠ산§릤§산ö
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Fig 4.§ 릤§산릤§牨릤䨸산§ꮠ䬬§×સè㮴èݧ릤릤§산§牨ú§ĜĀnĀĀ§산갬릤§䬬릤䨸牨§ § 릤§릤 䀸§산릤산릤§ 산ꮠ§ 牨§갬릤릤§릤산§릤 산§
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Inhibitory Activity of Lipid Peroxidation using the Laser-Based Photoacoustic Method§
§
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Improving and Applying Chemical Methodsî§
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ĆĨö§⟴ꮠ갬릤î§ööî§산§䬬䬬ꮠî§öŞöîöî§ĆĴĢĜî§Am. J.
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Isolationî§n§릤öî§nĜĨö§
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nĮö§산ꮠ䘐릤ö§산§䨸릤ꮠ갬릤î§öસöŪöî§ĆĴĴĴî§Free
Radicals in Biology and Medicineî§ 鯈 §
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Sunfilters Conf.,§ĐĢĐĴö§
ĐĀö§ꮠ牨牨릤牨산öö릤릤牨산䬬î§öî§Ūꮠ䬬릤䬬䨸î§⟴öî§ 릤§ꮠ릤§릤릤î§⟴öî§릤§䨸ꮠî§öî§સ릤ꮠ릤䬬î§સöî§ 산릤§ööસöî§릤ꮠ䬬릤산§산릤î§Űööî§ ĆĴĴĴî§ðꮠ§⟴䨸ꮠ릤䬬§ §쀔§Ŷ 릤䬬§ꮠ§䨸牨산§⟴ꮠ§ 쀔䬬ꮠ갬§ 산䨸䬬ꮠ䬬î§Proc. Eur. UV Sunfilters
Conf.î§ĆĴnĜö§
ĐĆö§Ű릤ꮠ䬬§ 산§Ş 산산䬬릤î§öŤö§ nĀĀĜî§Oxidation an
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