Lampiran 1. Hasil analisis kandungan asam lemak dari minyak alpukat

(1)

(2)

(3)

- asam stearat

- sorbitol

- setil alkohol

- propilen glikol

- dimasukkan ke dalam cawan

- trietanolamin

penguap

- metil paraben

- dilebur di atas penangas air pada suhu 70°C - dilarutkan dalam air

panas yg telah ditakar

pada suhu 70°C

- digerus konstan di dalam lumpang panas

- ditambahkan 3 tetes pengaroma

Fase air

Fase minyak

Fase minyak + fase air

(4)

- asam stearat

- sorbitol

- setil alkohol

- propilen glikol

- dimasukkan ke dalam cawan

- trietanolamin

penguap

- metil paraben

- dilebur di atas penangas air pada suhu 70°C - dilarutkan dalam air

panas yg telah ditakar

pada suhu 70°C

- digerus konstan di dalam lumpang panas

- ditambahkan minyak alpukat

- ditambahkan 3 tetes pewangi

Fase air

Fase minyak

Fase minyak + Fase air

(5)

Keterangan : A: Moisture Checker (Aramo-SG), B: Skin Analyzer (Aramo-SG),

C: pH meter (Hanna Instrument)

A

B

(6)

(7)

-setelah selesai dibuat

-setelah penyimpanan 12 minggu

Keterangan: Krim A: blanko, Krim B: krim minyak alpukat 5%, Krim C: krim

minyak alpukat 10%, Krim D: krim minyak alpukat 15%, Krim E: krim minyak

alpukat 20%.

Krim A

Krim B

B

Krim C

Krim D

D

Krim E

Krim A

Krim B

Krim C

Krim D

Krim E

(8)

Gambar uji homogenitas sediaan

Gambar uji penentuan tipe emulsi

Keterangan: Krim A: blanko, Krim B: krim minyak alpukat 5%, Krim C: krim

minyak alpukat 10%, Krim D: krim minyak alpukat 15%, Krim E: krim minyak

alpukat 20%.

Krim A

Krim B

Krim C

Krim D

Krim E

(9)

 Kadar air (moisture)

Kondisi awal :

Pemulihan minggu I :

Pemulihan minggu II :

Pemulihan minggu III :

(10)

 Kehalusan (evenness)

Kondisi awal :

Pemulihan minggu I :

(11)

Pemulihan minggu III :

(12)

 Noda (spot)

Kondisi awal :

(13)

Pemulihan minggu II :

(14)

(15)

 Keriput (wrinkle)

Kondisi awal :

(16)

Pemulihan minggu II :

(17)

(18)

SURAT PERNYATAAN PERSETUJUAN IKUT SERTA DALAM

PENELITIAN

Saya yang bertandatangan dibawah ini :

Nama

:

Umur

:

Alamat

:

Telah mendapat penjelasan secukupnya bahwa saya akan melakukan uji krim

minyak alpukat terhadap kulit sebagai sediaan krim anti-aging. Setelah

mendapat penjelasan secukupnya tentang manfaat penelitian ini dan efek

sampingnya, maka saya menyatakan SETUJU untuk ikut serta dalam penelitian

dari Cut Putri Arhandhi dengan judul “FORMULASI DAN UJI EFEK

ANTI-AGING DARI KRIM YANG MENGANDUNG MINYAK ALPUKAT

(Avocado oil), sebagai usaha untuk mengetahui apakah sediaan krim anti-aging

dari minyak alpukat yang dihasilkan mampu atau tidak dalam memulihkan kulit

yang telah mengalami penuaan. Saya menyatakan sukarela dan bersedia untuk

mengikuti prosedur penelitian yang telah ditetapkan.

Persetujuan ini saya buat dengan penuh kesadaran dan tanpa paksaan dari pihak

manapun. Demikian surat pernyataan ini dibuat untuk dapat dipergunakan

sebagaimana mestinya.

Medan, 27 November 2014

Peneliti,

Sukarelawan,

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1. Kadar air (moisture)

ANOVA

Sum of Squares df Mean Square F Sig. Sebelum Between Groups 2.500 5 .500 1.500 .261

Within Groups 4.000 12 .333

Total 6.500 17

MingguI Between Groups 5.111 5 1.022 2.300 .110

Total 10.444 17

MingguII Between Groups 10.944 5 2.189 2.627 .079

Total 20.944 17

MingguIII Between Groups 15.611 5 3.122 6.244 .004

Total 21.611 17

MingguIV Between Groups 17.778 5 3.556 5.818 .006

(20)

Sebelum

PEMULIHAN N

Subset for alpha = 0.05 1 Tukey HSDa Formula A 3 27.67 Formula B 3 27.67 Formula C 3 28.33 Formula D 3 28.33 Formula E 3 28.33 Formula F 3 28.67 Sig. .339

Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.

MingguI

PEMULIHAN N

Subset for alpha = 0.05 1 Tukey HSDa Formula A 3 29.67 Formula B 3 30.00 Formula C 3 30.33 Formula D 3 30.67 Formula E 3 30.67 Formula F 3 31.33 Sig. .082

(21)

MingguII

PEMULIHAN N

Subset for alpha = 0.05

1 2 Tukey HSDa Formula A 3 29.67 Formula B 3 31.00 31.00 Formula C 3 31.00 31.00 Formula D 3 31.00 31.00 Formula E 3 31.33 31.33 Formula F 3 32.33 Sig. .506

MingguIII

PEMULIHAN N

1 2 3 Tukey HSDa Formula A 3 30.33 Formula B 3 30.67 30.67 Formula C 3 32.00 32.00 32.00 Formula D 3 32.00 32.00 32.00 Formula E 3 32.33 32.33 Formula F 3 33.00 Sig. .538

(22)

MingguIV

PEMULIHAN N

1 2 Tukey HSDa Formula A 3 30.33 Formula B 3 31.67 31.67 Formula C 3 32.33 32.33 Formula D 3 32.67 Formula E 3 33.00 Formula F 3 33.33 Sig. .168

(23)

2. Kehalusan (evenness)

ANOVA

Sum of Squares df Mean Square F Sig. Sebelum Between Groups 60.944 5 12.189 1.180 .375 Within Groups 124.000 12 10.333 Total 184.944 17 MingguI Between Groups 44.278 5 8.856 1.147 .389 Within Groups 92.667 12 7.722 Total 136.944 17 MingguII Between Groups 32.278 5 6.456 .993 .462 Within Groups 78.000 12 6.500 Total 110.278 17 MingguIII Between Groups 67.778 5 13.556 1.807 .186 Within Groups 90.000 12 7.500 Total 157.778 17 MingguIV Between Groups 155.611 5 31.122 4.554 .015 Within Groups 82.000 12 6.833 Total 237.611 17

(24)

Sebelum

PEMULIHAN N

Subset for alpha = 0.05 1 Tukey HSDa Formula F 3 38.67 Formula E 3 39.33 Formula D 3 40.33 Formula C 3 41.33 Formula B 3 41.67 Formula A 3 44.33 Sig. .322

MingguI

PEMULIHAN N

(25)

MingguII

PEMULIHAN N

MingguIII

PEMULIHAN N

(26)

MingguIV

PEMULIHAN N

1 2 Tukey HSDa Formula F 3 29.00 Formula E 3 29.33 Formula D 3 33.00 33.00 Formula C 3 33.33 33.33 Formula B 3 34.00 34.00 Formula A 3 37.67 Sig. .310

(27)

3. Besar pori (pore)

ANOVA

Sum of Squares df Mean Square F Sig. Sebelum Between Groups 21.778 5 4.356 .311 .897 Within Groups 168.000 12 14.000 Total 189.778 17 MingguI Between Groups 29.167 5 5.833 .418 .827 Within Groups 167.333 12 13.944 Total 196.500 17 MingguII Between Groups 142.500 5 28.500 4.622 .014 Within Groups 74.000 12 6.167 Total 216.500 17 MingguIII Between Groups 404.444 5 80.889 16.545 .000 Within Groups 58.667 12 4.889 Total 463.111 17 MingguIV Between Groups 489.778 5 97.956 18.367 .000 Within Groups 64.000 12 5.333 Total 553.778 17

(28)

Sebelum

PEMULIHAN N

MingguI

PEMULIHAN N

(29)

MingguII

PEMULIHAN N

1 2 Tukey HSDa Formula F 3 16.00 Formula E 3 18.67 18.67 Formula D 3 20.67 20.67 Formula C 3 22.00 22.00 Formula B 3 23.67 Formula A 3 24.00 Sig. .163

MingguIII

PEMULIHAN N

1 2 3 4 Tukey HSDa Formula F 3 10.67 Formula E 3 14.00 14.00 Formula D 3 17.33 17.33 Formula C 3 17.33 17.33 Formula B 3 23.33 23.33 Formula A 3 24.00 Sig. .999

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MingguIV

PEMULIHAN N

1 2 3 4 Tukey HSDa Formula F 3 8.00 Formula E 3 10.67 10.67 Formula D 3 14.00 14.00 14.00 Formula C 3 14.67 14.67 Formula B 3 20.00 20.00 Formula A 3 23.33 Sig. .518

(31)

4. Noda (spot)

ANOVA

Sum of Squares df Mean Square F Sig. Sebelum Between Groups 172.944 5 34.589 2.256 .115 Within Groups 184.000 12 15.333 Total 356.944 17 MingguI Between Groups 17.333 5 3.467 .156 .974 Within Groups 266.667 12 22.222 Total 284.000 17 MingguII Between Groups 189.778 5 37.956 3.023 .054 Within Groups 150.667 12 12.556 Total 340.444 17 MingguIII Between Groups 437.111 5 87.422 11.921 .000 Within Groups 88.000 12 7.333 Total 525.111 17 MingguIV Between Groups 860.500 5 172.100 24.586 .000 Within Groups 84.000 12 7.000 Total 944.500 17

(32)

Sebelum

PEMULIHAN N

MingguI

PEMULIHAN N

(33)

MingguII

PEMULIHAN N

MingguIII

PEMULIHAN N

1 2 Tukey HSDa Formula F 3 24.67 Formula E 3 27.00 Formula D 3 32.00 32.00 Formula C 3 35.33 Formula B 3 36.00 Formula A 3 38.33 Sig. .113

(34)

MingguIV

PEMULIHAN N

1 2 3 4 Tukey HSDa Formula F 3 18.33 Formula E 3 22.33 22.33 Formula D 3 27.33 27.33 Formula C 3 32.00 32.00 Formula B 3 35.00 Formula A 3 38.00 Sig. .472 .260 .322 .129

(35)

5. Keriput (wrinkle)

ANOVA

Sum of Squares df Mean Square F Sig. Sebelum Between Groups 316.944 5 63.389 2.145 .129 Within Groups 354.667 12 29.556 Total 671.611 17 MingguI Between Groups 448.278 5 89.656 4.546 .015 Within Groups 236.667 12 19.722 Total 684.944 17 MingguII Between Groups 102.944 5 20.589 .563 .727 Within Groups 438.667 12 36.556 Total 541.611 17 MingguIII Between Groups 91.111 5 18.222 1.929 .163 Within Groups 113.333 12 9.444 Total 204.444 17 MingguIV Between Groups 509.333 5 101.867 4.654 .014 Within Groups 262.667 12 21.889 Total 772.000 17

(36)

Sebelum

Pemulihan N

MingguI

Pemulihan N

1 2 Tukey HSDa Formula F 3 26.33 Formula E 3 27.67 27.67 Formula D 3 31.67 31.67 Formula C 3 34.67 34.67 Formula B 3 39.00 Formula A 3 39.00 Sig. .074

(37)

MingguII

Pemulihan N

MingguIII

Pemulihan N

(38)

MingguIV

Pemulihan N

1 2 Tukey HSDa Formula F 3 14.00 Formula E 3 16.00 16.00 Formula D 3 22.33 22.33 Formula C 3 24.33 24.33 Formula B 3 27.67 Formula A 3 27.67 Sig. .145 .083

(39)

6. Kedalaman keriput (wrinkle’s depth)

ANOVA

Sum of Squares df Mean Square F Sig. Sebelum Between Groups 1080.944 5 216.189 14.099 .000 Within Groups 184.000 12 15.333 Total 1264.944 17 MingguI Between Groups 988.944 5 197.789 11.597 .000 Within Groups 204.667 12 17.056 Total 1193.611 17 MingguII Between Groups 1082.278 5 216.456 29.517 .000 Within Groups 88.000 12 7.333 Total 1170.278 17 MingguIII Between Groups 866.278 5 173.256 10.465 .000 Within Groups 198.667 12 16.556 Total 1064.944 17 MingguIV Between Groups 1636.444 5 327.289 11.461 .000 Within Groups 342.667 12 28.556 Total 1979.111 17

(40)

Sebelum

PEMULIHAN N

1 2 3 Tukey HSDa Formula F 3 39.00 Formula E 3 42.33 Formula D 3 43.33 Formula C 3 47.00 47.00 Formula B 3 56.00 56.00 Formula A 3 60.67 Sig. .694

MingguI

PEMULIHAN N

1 2 3 Tukey HSDa Formula F 3 35.67 Formula E 3 40.00 Formula D 3 42.33 Formula C 3 43.33 43.33 Formula B 3 54.33 54.33 Formula A 3 56.00 Sig. .995

(41)

MingguII

PEMULIHAN N

1 2 3 4 Tukey HSDa Formula F 3 32.00 Formula E 3 33.67 Formula D 3 38.00 38.00 Formula C 3 42.33 42.33 Formula B 3 49.00 49.00 Formula A 3 53.33 Sig. .416

MingguIII

PEMULIHAN N

1 2 3 Tukey HSDa Formula F 3 28.00 Formula E 3 33.00 33.00 Formula D 3 33.33 33.33 Formula C 3 42.33 42.33 Formula B 3 45.33 Formula A 3 46.33 Sig. .827

(42)

MingguIV

PEMULIHAN N

1 2 3 Tukey HSDa Formula F 3 20.33 Formula E 3 24.00 Formula D 3 29.67 29.67 Formula C 3 42.00 42.00 Formula B 3 42.33 42.33 Formula A 3 44.33 Sig. .993