pengaruh inhibitor terhadap aktivitas enzim

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PERCOBAAN 9

PENGARUH INHIBITOR

TERHAD

AP AK

TIFIT

AS ENZIM

Oleh:

Kelompok 7

Sulimah

Vika Noer Baety

Y

uneta dwi Y

unisari

Zulfa Anifatul C

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Latar Belakang Latar Belakang

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Latar Belakang

Dewaa !n! "a#a!wa "engenal !n#!$!! en%!" &! &ala" el

"er'(akan 'at' "ekan!"e (engen&al!an reak!)reak! en%!"at!k &engan (elak' !n#!$!! &!e$'t !n#!$!t*r+ In#!$!t*r &a(at $er'(a

"*lek'l ata' !*n ,ang &a(at "eng#a"$at reak! (e"$ent'kan ata' (eng'ra!an k*"(lek ES (a&a reak! en%!"+ In#!$!t*r &!$e&akan

"en-a&! !n#!$!t*r t!&ak &a(at $al!k .!rre/er!$le0 &an !n#!$!t*r &a(at $al!k .re/er!$le0+In#!$!t*r &a(at $al!k ter&!r! &ar! t!ga -en! !n#!$!t*r1 ,a!t'2 !n#!$!t*r $era!ng .k*"(et!t!301 !n#!$!t*r t!&ak $era!ng

.n*nk*"(et!t!301 &an !n#!$!t*r $'kan $era!ng .'nk*"(et!t!30+ Dar! ek!an (engert!an &an -en! !n#!$!t*r1 "a#a!wa #an,a "e"a#a"! eke&ar k*ne( &an te*r!+ Pa&a#al 'nt'k "e"a#a"! le$!# &ala" tentang (engar'# !n#!$!t*r ter#a&a( akt!4ta en%!" &an (er!t!wa ,ang ter-a&! (a&a !n#!$!! k*"(et!t!3 ter#a&a( akt!4ta en%!"1

&!$'t'#kan 'at' ker-a n,ata &ar! "a#a!wa 'nt'k "e"$'kt!kan #al tere$'t &ar! te*r! ,ang a&a &engan &!a&akann,a (er5*$aan tentang (engar'# !n#!$!t*r ter#a&a( akt!4ta en%!"+

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T'-'an Per5*$aaan

Setela# "eng!k't! (er5*$aan !n!1 "a#a!wa &!#ara(kan2 6+ Ma"(' "e"a#a"! (engar'# !n#!$!t*r natr!'" "al*nat

ter#a&a( akt!4ta en%!" 'k!nat &e#!&r*genae 7+ Ma"(' "enga"at! ter-a&!n,a (er!t!wa !n#!$!!

k*"(et!t!3 ter#a&a( akt!4ta en%!" 'k!nat &e#!&r*genae+

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Pr!n!( Per5*$aan

Pa&a (er5*$aan !n! akan &!lak'kan '-! (engar'# !n#!$!t*r ter#a&a( akt!4ta en%!"1 &engan '$trat natr!'" 'k!nat &an !n#!$!t*r natr!'" "al*nat ,ang akan $erk*"(et!! 'nt'k "eng!kat !! akt!3 en%!" 'k!nat &e#!&r*genae ,ang &!&a(atkan &ar!

#*"*genat #at! a(!+

Ket!ka '$trat 'k!nat "eng!kat !! akt!3 en%!" 'k!nat

&e#!&r*genae "aka akan ter*k!&a! &an ter$ent'k 3'"arat ,ang &!tan&a! &engan tere&'k!n,a "et!len $!r' &ar! $erwarna $!r'

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R'"'an Maala#

6+ Baga!"ana (engar'# !n#!$!t*r natr!'" "al*nat ter#a&a( akt!4ta en%!" 'k!nat &e#!&r*genae8

7+ Baga!"anaka# (er!t!wa !n#!$!! k*"(et!t!3 ter#a&a( akt!4ta en%!" 'k!nat &e#!&r*genae8

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Met*&e

6+ Ta$'ng reak! 7+ Batang Penga&'k + P!(et tete :+ Gela 'k'r ! "etilen #lue $%

&! Bu'er fosfat ()" *p+ 7),-.! Natrium suksinat () " ,! Natrium malonat () " $! +omo/enat hati 0! "inyak para1n 7! Akuades Alat Bahan

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Pr*e&'r

2a#un / ke Akuad es *m3-Bu'er fosfat *m3-Natriu m suksin at *m3-Natriu m malon at *m3-"etile n #iru *m3-+omo/ enat hati *m3-4ara1n *tetes-6 1; 7 7 ) ;1< 71< 6; 7 71= 7 7 ;17 ;1< 71< 6;  71> 7 7 ;1: ;1< 71< 6; : ;1> 7 : ;1: ;1< 71< 6; < ;1> 7 > ;1: ;1< 71< 6; > > 7 ) ) ;1< 71< 6;

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Anal!! (r*e&'r

6+ Perta"a &!"a'kkan "et!len $!r' ,ang $ert'-'an e$aga! !n&!kat*r tela# ter-a&!n,a (eng*k!&a!an 'k!nat "en-a&! 3'"arat *le# en%!" ,ang &!g'nakan ,akn! en%!" 'k!nat &e#!&r*genae ,ang ter&a(at (a&a #*"*genat #at!+

Mekan!"en,a "elal'! reak! re&*k e&er#ana "et!len $!r' akan tere&'k! "en-a&! $erwarna ('t!# &an 'k!nat akan ter*k!&a!+

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7+ D!ta"$a#kan a!r 'nt'k "elar'tkan en,awa)en,awa ,ang akan &!5a"('rkan+ ?arna "et!len $!r' agak "e"'&ar+

+ D!ta"$a#kan $'@er 3*3at ;16 M (a&a (H 1: ,a!t'

$ert'-'an 'nt'k "e"(erta#ankan (H+ (H *(t!"'" (a&a en%!" 'k!nat &e#!&r*genae karena en%!" tere$'t ta$!l (a&a (H 1:

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:+ Pena"$a#an natr!'" 'k!nat ;16 M $ert'-'an e$aga! '$trat ,ang nant!n,a "eng!kat !! akt!3 &ar! en%!" 'k!nat &e#!&r*genae+

<+ Pena"$a#an natr!'" "al*nat &!g'nakan e$aga! !n#!$!t*r ata' &a(at "eng#a"$at ker-a &ar! en%!" 'k!nat

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>+ Pena"$a#an #*"*genat #at! e$aga! en%!" 'k!nat &e#!&r*genae+ Hat! a(! &!(!l!# karena &! &ala" #at! ter&a(at $an,ak en%!" ,ang &!g'nakan *le# 'at'

"ak#l'k #!&'( 'nt'k "elak'kan "eta$*l!"en,a &engan $enar1 "aka &engan "engg'nakan #*"*genat #at! a(!1 en%!" ,ang &!g'nakan le$!# $an,ak+

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+ H*"*genat #at! #ar' #*"*gen agar e"'a en%!" ,ang &!ektrak &ar! #at! a(! &a(at kel'ar &an "erata+

=+ D!a&'k #!ngga #*"*gen $ert'-'an agar e"'a 5a"('ran #*"*gen &an k*nentra! 5a"('ran a"a+

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9+ Selan-'tn,a (ena"$a#an (ara4n $er3'ng! 'nt'k

"eng#!n&ar! k*ntak '&ara $e$a &egan lar'tan karena

(ara4n "e"!l!k! "aa -en! ,ang le$!# r!ngan &ar!(a&a a!r e#!ngga akan ter$ent'k la(!an (ara4n &! ata la(!an

lar'tan 1e#!ngga &engan a&an,a (ara4n 1 *k!gen &ar! '&ara $e$a t!&ak akan "a'k ke &ala" lar'tan karena "et!len $!r' tere&'k! e5ara 5e(at &a(at ter*k!&a! ke"$al! &engan ke#a&!ran *k!gen '&ara+

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6;+ Setela# !t' &!lak'kan !nk'$a! (a&a '#' =;C+ S'#' !n!

&!g'nakan karena "er'(akan '#' *(t!"'" &ar! ker-a en%!" 'k!nat &e#!&r*genae+

66+ D!5atat wakt'n,a &an &!a"at! warnan,a $ert'-'an 'nt'k "engeta#'! "ana ,ang (al!ng 5e(at &an (al!ng la"$at 'nt'k "er'$a# warna $!r' "en-a&! warna e(ert! lar'tan tan&ar+

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Ha!l Per5*$aan

2a#u n/ *5ata kelompok 7-+ilan/nya warna menit ke6 *5ata kelompok $-+ilan/nya warna menit ke6 6 7;  7 : <>  = >> : 7= < < : 9 > ) ) 5ata  Data 7

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Pe"$a#aan

6+ Ak'ra! Data

Dar! #a!l (er$an&!ngan &ata &'(l*1 ,akn! &ata &ar! kel*"(*k ka"! .kel*"(*k 0 &engan kel*"(*k <1 &!&a(atkan anal!a &ata e$aga! $er!k't+

a+ 2a#un/ Volume su#strat * natrium suksinat-Volume inhi#itor *natrium malonat-aktu *kelompok 7-aktu *kelompok $-6 7 "L ; "L 7; "en!t  "en!t 7 7 "L ;17 "L : "en!t <> "en!t  7 "L ;1: "L = "en!t >> "en!t

Da(at &!!"('lkan $a#wa semakin sedikit inhibitor yang ditambahkan maka laju reaksinya semakin cepat dengan konsentrasi substrat yang dibuat tetap.

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$+

2a#u n/ Volume su#strat * natrium suksinat-Volume inhi#itor *natrium malonat-aktu *kelompok 7-aktu *kelompok $- 7 "L ;1: "L = "en!t >< "en!t : : "L ;1: "L 7= "en!t < "en!t

Pengar'# '$trat ter#a&a( akt!4ta en%!" ,a!t'

e"ak!n $ear -'"la# '$trat ,ang &!ta"$a#kan "aka e"ak!n 5e(at la-' reak! (a&a akt!4ta en%!" 'k!nat &e#!&r*genae e#!ngga (er'$a#an warna e"ak!n

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5+ Unt'k ta$'ng < t!&ak &!$an&!ngkan karena -'"la#

keel'r'#an t!&ak a"a &engan ta$'ng 6):+ Unt'k ta$'ng 6): -'"la# t*tal /*l'"e ,a!t' 1< "L e&angkan ta$'ng < e$an,ak 91< "L+

&+ Unt'k ta$'ng > &!g'nakan e$aga! tan&ar warna e$aga! (e"$an&!ng &engan ta$'ng 6)<+

Dar! anal!a &ata tere$'t &a(at &!katakan $a#wa &ata &ar! kel*"(*k ka"! "e"!l!k! ak'ra! ,ang 5'k'( t!ngg!+

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7+ Pengar'# !n#!$!t*r natr!'" "al*nat ter#a&a( akt!4ta en%!"+

Dar! (er5*$aan ,ang tela# &!lak'kan &a(at &!!"('lkan $a#wa natr!'" "al*nat e$aga! !n#!$!t*r $er(engar'#

ter#a&a( akt!4ta en%!" 'k!nat &e#!&r*genae ,ang ter&a(at &! &ala" #*"*genat #at! ,akn! !n#!$!t*r &a(at "eng#a"$at ker-a en%!" 'k!nat &e#!&r*genae &engan "eng!kat !! akt!3 en%!" karena nat!r'" "al*nat "e"!l!k! tr'kt'r t!ga &!"en! ,ang a"a &engan natr!'" 'k!nat+

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+ en! !n#!$!! &ala" (er5*$aan+

en! !n#!$!! (a&a (er5*$aan !n! a&ala# !n#!$!!

k*"(et!t!3 karena "*lek'l !n#!$!t*r ,ang &!g'nakan !ala# natr!'" "al*nat ,ang "en,er'(a! "*lek'l '$trat

natr!'" 'k!nat e#!ngga &a(at $erk*"(et!! &engan

'$trat natr!'" 'k!nat &ala" "eng!kat !! akt!3 en%!" 'k!nat &e#!&r*genae1 teta(! t!&ak &a(at "eng#a!lkan (r*&'k+

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Se5ara "ate"at!k !n#!$!! !n! &a(at &!n,atakan e$aga!2

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Natr!'" "al*nat "e"!l!k! tr'kt'r &an "'atan ,ang a"a &engan '$trat n*r"al natr!'" 'k!nat1 teta(! natr!'" "al*nat t!&ak &a(at &!&e#!&r*gena! *le# en%!"+

NaOOCCH7COONa .natr!'" "al*nat0 NaOOCCH7CH7COONa .natr!'" 'k!nat0

Bearn,a (engar'# !n#!$!t*r k*"(et!t!3 &!tent'kan *le#

k*nentra! !n#!$!t*r1 k*nentra! '$trat &an a4n!ta relat!3 '$trat &an !n#!$!t*r ter#a&a( en%!"+ Pengar'# !n#!$!t*r

k*"(et!t!3 &a(at &!ata! &engan '$trat $erle$!#+ .S'ant!1 7;60

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Ke!"('lan

Dar! (er5*$aan ,ang tela# &!lak'kan &a(at &!!"('lkan $a#wa2

6+ Natr!'" "al*nat e$aga! !n#!$!t*r $er(engar'# ter#a&a( akt!4ta en%!" 'k!nat &e#!&r*genae ,ang ter&a(at &! &ala" #*"*genat #at! ,akn! !n#!$!t*r &a(at "eng#a"$at ker-a en%!" 'k!nat &e#!&r*genae &engan "eng!kat !! akt!3 en%!"+

7+ Per!t!wa ,ang ter-a&! (a&a !n#!$!! k*"(et!t!3 ter#a&a( akt!4ta en%!" !ala# en%!" ,ang tela# "eng!kat !n#!$!t*r t!&ak &a(at $ereka! &engan '$trat 'nt'k "eng#a!lkan (r*&'k1 e&angkan en%!" ,ang tela# $er!katan &engan

'$trat t!&ak &a(at $er!katan &engan !n#!$!t*r1 akan teta(! "eng#a!lkan #a!l "eng!k't! reak! k!"!a $er!k't !n!2

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Ft Per5*$aan

am#ar ! Lar'tan "et!len $!r' 8am#ar !& Lar'tan "et!len $!r' &an ak'a&e &ala" ta$'ng 6)< .&ar! k!r!0

8am#ar !. Lar'tan 6)< e$el'" 'k!nat "en-a&! 3'"arat &engan 6 ta$'ng

8am#ar !, Lar'tan 6)< (erla#an $er'$a# &ar! 'k!nat "en-a&!

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pengaruh inhibitor terhadap aktivitas enzim

PERCOBAAN 9

PERCOBAAN 9