Monica Adyah Permata 22010111120033 Lap.KTI Bab8

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LAMPIRAN

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Lampiran 3. Informed Consent (lanjutan)

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Pengecatan : Hematoksilin Eosin

1.  Tali pusat dipotong sepanjang 2 cm.

 Potongan tali pusat diimasukkan ke dalam pot penampung berisi buffer formalin 10% dengan perbandingan volume jaringan: formalin adalah 1:10.

 Pemberian identitas pot penampung jaringan.

 Pengiriman sampel ke laboratorium sentral bagian Patologi Anatomi.

2.  Jaringan mentah dipotong menjadi 3 bagian (dipilih bagian yang sesuai dengan kriteria penelitian).

 Potongan jaringan dimasukkan dalam kaset.

 Kaset jaringan kemudian direndam kembali dalam wadah berisi formalin 10% hingga Processing jaringan siap dilakukan.

3. Kaset jaringan dimasukkan ke dalam keranjang alat processing jaringan yakni Microm STP 120 (1 keranjang bisa memuat hingga 80 kaset). Processing jaringan selama 17, 5 jam.

Microtom STP 120 terdiri dari beberapa tabung, yaitu :

 Formalin 10% 1, lama perendaman 2 jam

 Formalin 10% 2, lama perendaman 2 jam

 Alkohol 70 % , lama perendaman 1 jam

 Alkohol 80%, lama perendaman 1 jam

 Alkohol 96%, lama perendaman 2 jam

 Etanol, lama perendaman 2 jam

 Xylol, lama perendaman 1 jam

 Parafin 1, lama perendaman 2 jam

Tujuan processing:

 Menghilangkan cairan pada jaringan (dehidrasi dg alkohol bertingkat yakni : alkohol 70%,80%,96% dan etanol).

 Xylol untuk clearing.

 Parafin untuk mematangkan jaringan.

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5.  Blok jaringan dipotong dengan mikrotom (ketebalan 2 mikron).

 Hasil pemotongan dimasukkan dalam air hangat agar

mengembang, kemudian jaringan ditempelkan pada kaca objek.

 Preparat diberi identitas

6.  Preparat dipanaskan dalam oven 70 derajat selama 10 menit (untuk melelehkan parafin)

 Setelah selesai preparat dinginkan, kemudian ditata pada rak jaringan untuk persiapan proses pengecatan.

7. Pengecatan dilakukan dengan alat Veristein dengan total waktu 2,5 jam. Veristein terdiri dari beberapa tabung, yaitu :

 Xylol 1, lama perendaman 10 menit

 Etanol , lama perendaman 3 menit

 Alkohol 96% , lama perendaman 3 menit

 Alkohol 80% , lama perendaman 3 menit

 Alkohol 70%, lama perendaman 3 menit

 Aqua

 Hematoksilin , lama perendaman 15 menit

 HCL, lama perendaman 1 menit

 Bruing, lama perendaman 10 menit

 Aqua, lama perendaman 3 menit

 Eosin, lama perendaman 3 menit

 Etanol, lama perendaman 3 menit

Fungsi masing-masing cairan :

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 Aqua, merupakan pelarut hematoksilin fungsi menyamakan pH dengan hematoksilin

 Hematoksilin berfungsi untuk mewarnai inti

 HCL berfungsi untuk mencegah pewarnaan berlebih, agar pewarnaan tidak terlalu biru

 Bruing berfungsi untuk memperjelas warna inti

 Alkohol 50% dan 70%, merupakan pelarut eosin

 Eosin, berfungsi untuk mewarnai sitoplasma

 Alkohol bertingkat, untuk rehidrasi jaringan

 Xylol untuk proses clearing

8. Preparat yang sudah diwarnai ditetesi Ez Mount kemudian ditutup dengan deck glass .

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Lampiran 6. Hasil analisis statistik

Tests of N ormality

Kolmogorov-Smirnova _Shapiro-Wilk

a. Lilliefors Significance Correction

T-Test

Group Statistics

Diagnosis N Mean Std. Deviation Std. Error Mean

Umur ibu

Preeklampsia berat 16 33.00 6.563 1.641

Kehamilan normotensi 16 28.31 5.582 1.396

Independent Samples Test

t-test for Equality of Means

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Lampiran 6. Hasil analisis statistik (lanjutan)

Mann-Whitney Test

Test Statisticsa

Umur kehamilan Cara persalinan Paritas

Mann-Whitney U 85.500 123.500 80.000

Wilcoxon W 221.500 259.500 216.000

Z -1.697 -.182 -2.156

Asymp. Sig. (2-tailed) .090 .855 .031

Exact Sig. [2*(1-tailed Sig.)] .110b _.867b _.073b

a. Grouping Variable: Diagnosis

b. Not corrected for ties.

Means

Report

Diagnosis Umur ibu Umur kehamilan

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a. Lilliefors Significance Correction

Mann-Whitney Test

Test Statisticsa

Luas area tali pusat

Luas area pembuluh

darah

Luas area Wharton's jelly

Mann-Whitney U 61.000 41.000 67.000

Wilcoxon W 197.000 177.000 203.000

Z -2.525 -3.279 -2.299

Asymp. Sig. (2-tailed) .012 .001 .022

Exact Sig. [2*(1-tailed Sig.)] .011b _.001b _.021b

a. Grouping Variable: Diagnosis b. Not corrected for ties.

Means

Case Processing Summary

Cases

Included Excluded Total N Percent N Percent N Percent Luas area tali pusat *

Diagnosis 32 100.00% 0 0.00% 32 100.00%

Luas area pembuluh darah *

Diagnosis 32 100.00% 0 0.00% 32 100.00%

Luas area Wharton's jelly *

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Luas area Wharton's jelly

Preeklampsia berat

Mean 85.2857 12.6448 72.6410

Std.

Deviation 38.14485 4.23282 35.68374

Median 81.9518 11.8321 70.1197

Minimum 30.81 7.19 23.62

Deviation 16.45145 2.27845 15.19417

Median 46.3182 7.8793 38.2380

Deviation 33.45881 3.95647 30.74823

Median 53.4325 9.0636 45.7462

Minimum 30.80 6.41 23.62

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Lampiran 7. Dokumentasi

Lampiran 6. Dokumentasi (lanjutan)

Lampiran 6. Dokumentasi (lanjutan) Gambar 9. Pengambilan sampel tali pusat

Gambar 10. Processing jaringan

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Lampiran 8. Biodata mahasiswa

Identitas

Nama : Monica Adyah Permata

NIM : 22010111120033

Tempat/tanggal lahir : Karawang, 25 September 1992 Jenis kelamin : Wanita

Alamat : Gg. Madi 2, RT01/01, Ds.Pucung, Kec. Kotabaru, Kab. Karawang, Jawa Barat

Nomor Hp : 083875162522

e-mail : monicaadyahpermata@gmail.com

Riwayat Pendidikan Formal 2. Ketua Financial ROHIS KU Tahun : 2012 s.d. 2013 3. Paduan suara mahasiswa KU Tahun : 2011 s.d. 2014 4. Volunteer Bina Antar Budaya Tahun : 2011 s.d. sekarang Pengalaman Penelitian