Lampiran 1. Kurva Standar Protein Larutan Bardfrod
Commasive blue ditimbang sebanyak 0,01 gram kemudian dilarutkan ke dalam 5 ml etanol 95% dan ditambah dengan 10 ml asam fosfor. Larutan selanjutnya disaring dengan menggunakan kertas saring dan disimpan dalam botol gelap pada suhu rendah. Sebelum digunakan, larutan harus diencerkan terlebih dahulu sebanyak lima kali (Bollag & Stuart, 1991)
Pembuatan Kurva Standar
Bovine Serum Albumin (BSA) ditimbang sebanyak 100 mg kemudian dilarutkan ke dalam 100 ml ddH2O dan larutan tersebut dinamakan larutan stok BSA 1 mg/ml.
Selanjutnya, larutan stok BSA dibuat seri pengenceran larutan standar dengan konsentrasi 0 mg/ml, 0,1 mg/ml, 0,2 mg/ml, 0,3 mg/ml, 0,4 mg/ml, 0,5 mg/ml, 0,6 mg/ml, 0,7 mg/ml, 0,8 mg/ml, 0,9 mg/ml, dan 1 mg/ml. Dari masing-masing konsentrasi larutan standar diambil sebanyak 0,1 ml, lalu ditambahkan 5 ml larutan Bradford. Setelah itu, larutan didiamkan selama 5 menit, dan diukur absorbansinya pada panjang gelombang 595 nm (Bradford, 1976).
Lampiran 2. Komposisi Reagen Elektroforesis Protein Larutan Stok (Stock Solutions)
Bahan-bahan yang digunakan dalam pembuatan larutan stok adalah 2M Tris-HCl (pH 8,8) 100 ml, 1 Tris-HCl (pH 6,8) 100 ml, 10% SDS 100 ml, 50% gliserol 100 ml, dan 1% bromophenolblue 10 ml.
Larutan Kerja (Working Solutions)
Nama Larutan Komposisi
Larutan A
(Acrylamide Stock Solution), 100 ml 30% acrylamide, 0,8% bis-acrylamide
29,2 gram acrylamide
0,8 gram bis-acrylamide
Larutan B
(4x Separating Gel Buffer), 100 ml
75 ml 2M Tris-HCl (pH 8,8) --- 1,5 M 4 ml 10% SDS --- 0,4 % 21 ml ddH2O
Larutan C
(4x Stacking Gel Buffer), 100 ml
50 ml 1M Tris-HCl (pH 6,8) --- 0,5 M 4 ml 10% SDS --- 0,4 % 46 ml ddH2O
10% Ammonium Persulphate
(APS)
0,5 gram APS 5 ml DDH2O
Buffer Elektroforesis 3 gr Tris --- 25Mm 14,4 gram glycine --- 192Mm 1 gram SDS --- 0,1% ddH2O sampai 1 liter
Sampel Buffer 3,028 gram Tris
0,25 ml larutan C 0,4 ml 10% SDS 25 ml gliserol
0,2 mg bromophenol blue
0,031 gram Dithiothreitol (DTT)
Larutan 12,5% Separating Gel Buffer (dalam 10 ml)
Larutan Jumlah
Larutan acrylamide 4,2 ml
4x Separating gel buffer 2,5 ml
10% SDS 100 µl
ddH2O 3,2 ml
10% Ammonium Persulphate (APS) 50 µl
Larutan 4x Stacking Gel Buffer (dalam 10 ml)
Larutan Jumlah
Larutan acrylamide 2,66 ml
4x stacking gel buffer 2,5 ml
10% SDS 0,1 ml
ddH2O 6 ml
10% Ammonium Persulphate (APS) 50 µl
Lampiran 3. Kurva Standar BSA (Bovine Serum Albumin) Low Moleculer Weight
Lampiran 4. Hasil Pengolahan SPSS Tingkat Kekerasan (Hardness) pada Adonan Tepung Terigu
Hard ness
Duncana
6 183,0533
6 220,4083
6 310,7017
1,000 1,000 1,000 Perlakuan
Adonan Tpeung Terigu Kontrol Adonan Tepung Terigu + Jahe 1,5% Adonan Tepung Terigu + Jahe 3% Sig.
N 1 2 3
Subset f or alpha = .05
Means f or groups in homogeneous subsets are display ed. Uses Harmonic Mean Sam ple Size = 6,000.
Lampiran 5. Hasil Pengolahan SPSS Karakteristik Fisik Roti Kukus a. Tingkat Kekerasan (Hardness)
Roti Kukus Jahe 0%
Hard ness Roti Ku kus Jahe 0%
Duncana,b
6 115,9317
6 176,0700
6 370,4967
1,000 1,000 1,000 Tahapan
Proof ing Mixing St eam ing Sig.
N 1 2 3
Subset
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = 40,777. Uses Harmonic Mean Sample Size = 6,000. a.
Alpha = , 05. b.
Roti Kukus Jahe 1,5%
Hardness Roti Kukus Jahe 1,5%
Duncana,b
6 143,8850
6 217,5817
6 712,6117
1,000 1,000 1,000
Tahapan Proof ing Mixing St eam ing Sig.
N 1 2 3
Subset
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = 261,490. Uses Harmonic Mean Sample Size = 6,000. a.
Alpha = , 05. b.
Roti Kukus Jahe 3%
Hardness Roti Kukus Jahe 3%
Duncana,b
6 168,1267
6 265,6117
6 899,9483
1,000 1,000 1,000
Tahapan Proof ing Mixing St eam ing Sig.
N 1 2 3
Subset
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = 57,050. Uses Harmonic Mean Sample Size = 6,000. a.
Mixing
Hardness Mixing
Duncana,b
6 176,0700
6 217,5817
6 265,6117
1,000 1,000 1,000
Perlakuan
Roti Kukus Kont rol Roti Kukus Jahe 1,5% Roti Kukus Jahe 3% Sig.
N 1 2 3
Subset
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = 56,683. Uses Harmonic Mean Sample Size = 6,000. a.
Alpha = , 05. b.
Proofing
Hard ness Proo fing
Duncana,b
6 115,9317
6 143,8850
6 168,1267
1,000 1,000 1,000
Perlakuan
Roti Kukus Kont rol Roti Kukus Jahe 1,5% Roti Kukus Jahe 3% Sig.
N 1 2 3
Subset
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = 22,232. Uses Harmonic Mean Sample Size = 6,000. a.
Alpha = , 05. b.
Steaming
Hardness Steaming
Duncana,b
6 370,4967
6 712,6117
6 899,9483
1,000 1,000 1,000
Perlakuan
Roti Kukus Kont rol Roti Kukus Jahe 1,5% Roti Kukus Jahe 3% Sig.
N 1 2 3
Subset
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = 280,403. Uses Harmonic Mean Sample Size = 6,000. a.
b. Warna
L*
L
Duncana
6 75,1567
6 76,3233
6 84,1850
1,000 1,000 1,000
Perlakuan
Roti Kukus Jahe 3% Roti Kukus Jahe 1,5% Roti Kukus Kont rol Sig.
N 1 2 3
Subset f or alpha = . 05
Means f or groups in homogeneous subset s are display ed. Uses Harmonic Mean Sample Size = 6,000.
a.
a*
a
Duncana
6 -2,7733
6 -2,3667
6 -,8083
1,000 1,000 1,000
Perlakuan
Roti Kukus Kont rol Roti Kukus Jahe 1,5% Roti Kukus Jahe 3% Sig.
N 1 2 3
Subset f or alpha = . 05
Means f or groups in homogeneous subset s are display ed. Uses Harmonic Mean Sample Size = 6,000.
a.
b*
b
Duncana
6 20,4600
6 24,3767
6 27,7800
1,000 1,000 1,000
Perlakuan
Roti Kukus Kont rol Roti Kukus Jahe 3% Roti Kukus Jahe 1,5% Sig.
N 1 2 3
Subset f or alpha = . 05
Means f or groups in homogeneous subset s are display ed. Uses Harmonic Mean Sample Size = 6,000.
c. Volume Pengembangan Roti Kukus Jahe 0%
Vo lume Duncana,b
6 31,2017
6 55,3967
6 81,6717
1,000 1,000 1,000 Tahapan
Mixing Proof ing St eam ing Sig.
N 1 2 3
Subset
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = ,792. Uses Harmonic Mean Sample Size = 6,000. a.
Alpha = , 05. b.
Roti Kukus Jahe 1,5%
Vo lume
Duncana,b
6 35,4583
6 47,7350
6 74,2233
1,000 1,000 1,000 Tahapan
Mixing Proof ing St eam ing Sig.
N 1 2 3
Subset
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = ,761. Uses Harmonic Mean Sample Size = 6,000. a.
Alpha = , 05. b.
Roti Kukus Jahe 3%
Vo lume Duncana,b
6 39,4717
6 51,8533
6 77,4100
1,000 1,000 1,000 Tahapan
Mixing Proof ing St eam ing Sig.
N 1 2 3
Subset
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = ,894. Uses Harmonic Mean Sample Size = 6,000. a.
Mixing
Volume
Duncana,b
6 31,2017
6 35,4583
6 39,4717
1,000 1,000 1,000
Perlakuan
Roti Kukus Kont rol Roti Kukus Jahe 1,5% Roti Kukus Jahe 3% Sig.
N 1 2 3
Subset
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = ,778. Uses Harmonic Mean Sample Size = 6,000. a.
Alpha = , 05. b.
Proofing
Volume
Duncana,b
6 47,7350
6 51,8533
6 55,3967
1,000 1,000 1,000
Perlakuan
Roti Kukus Jahe 1,5% Roti Kukus Jahe 3% Roti Kukus Kont rol Sig.
N 1 2 3
Subset
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = ,801. Uses Harmonic Mean Sample Size = 6,000. a.
Alpha = , 05. b.
Steaming
Vo lume
Duncana,b
6 74,2233
6 77,4100
6 81,6717
1,000 1,000 1,000
Perlakuan
Roti Kukus Jahe 1,5% Roti Kukus Jahe 3% Roti Kukus Kont rol Sig.
N 1 2 3
Subset
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = ,868. Uses Harmonic Mean Sample Size = 6,000. a.
Lampiran 6. Hasil Pengolahan SPSS Aktivitas Antioksidan pada Roti Kukus
Roti Kukus Jahe 0%
Anti oksidan
Duncana,b
6 2,9567
6 8,0750
6 11,4750
1,000 1,000 1,000
Tahapan St eam ing Proof ing Mixing Sig.
N 1 2 3
Subset
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = ,877. Uses Harmonic Mean Sample Size = 6,000. a.
Alpha = , 05. b.
Roti Kukus Jahe 1,5%
Anti oksidan
Duncana,b
6 9,3483
6 13,8067
6 21,6717
1,000 1,000 1,000
Tahapan St eam ing Proof ing Mixing Sig.
N 1 2 3
Subset
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = 3,089. Uses Harmonic Mean Sample Size = 6,000. a.
Alpha = , 05. b.
Roti Kukus Jahe 3%
Anti oksidan
Duncana,b
6 18,7217
6 26,3800
6 34,1617
1,000 1,000 1,000
Tahapan St eam ing Proof ing Mixing Sig.
N 1 2 3
Subset
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = 1,153. Uses Harmonic Mean Sample Size = 6,000. a.
Mixing
Anti oksidan
Duncana,b
6 11,4750
6 21,6717
6 34,1617
1,000 1,000 1,000
Perlakuan
Roti Kukus Kont rol Roti Kukus Jahe 1,5% Roti Kukus Jahe 3% Sig.
N 1 2 3
Subset
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = 1,327. Uses Harmonic Mean Sample Size = 6,000. a.
Alpha = , 05. b.
Proofing
Anti oksidan
Duncana,b
6 8,0750
6 13,8067
6 26,3800
1,000 1,000 1,000
Perlakuan
Roti Kukus Kont rol Roti Kukus Jahe 1,5% Roti Kukus Jahe 3% Sig.
N 1 2 3
Subset
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = 2,604. Uses Harmonic Mean Sample Size = 6,000. a.
Alpha = , 05. b.
Steaming
Anti oksidan
Duncana,b
6 2,9567
6 9,3483
6 18,7217
1,000 1,000 1,000
Perlakuan
Roti Kukus Kont rol Roti Kukus Jahe 1,5% Roti Kukus Jahe 3% Sig.
N 1 2 3
Subset
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares
The error term is Mean Square(Error) = 1,188. Uses Harmonic Mean Sample Size = 6,000. a.
