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The Effect of Addition of Lactobacillus plantarum S4512 on The Microbiological and Chemical Characteristics during Sorghum (Sorghum bicolor L. Moench) Fermentation | Utami | Agritech 9329 17309 1 PB

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THE EFFECT OF ADDITION OF

Lactobacillus plantarum

6217+(

MICROBIOLOGICAL AND CHEMICAL CHARACTERISTICS DURING SORGHUM

(

Sorghum bicolor

L. Moench) FERMENTATION

Tyas Utami

1

, Rifa Nurhayati

2

, Endang Sutriswati Rahayu

1

1'HSDUWPHQWRI)RRGDQG$JULFXOWXUDO3URGXFW7HFKQRORJ\)DFXOW\RI$JULFXOWXUDO7HFKQRORJ\*DGMDK0DGD8QLYHUVLW\ -O)ORUD1R%XODNVXPXU<RJ\DNDUWD

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Email: tys_utami@yahoo.com.sg

ABSTRACT

7KHDLPRIWKLVVWXG\ZDVWRLQYHVWLJDWHWKHSRSXODWLRQRIVHOHFWHGEDFWHULDDQGsome chemical characteristics during VRUJKXPIHUPHQWDWLRQZLWKWKHDGGLWLRQRILactobacillus plantarum 63URWHRO\WLFL. plantarum6LVRODWHG

IURPQDWXUDOVRUJKXPIHUPHQWDWLRQZDVDGGHGLQWRVRUJKXPIHUPHQWDWLRQ6RUJKXPÀRXUZDVPL[HGZLWKVWHULOHZDWHU ZYDQGWKHQZDVLQRFXODWHGZLWKYYDERXW9&)8POFXOWXUHRIL. plantarum6)HUPHQWDWLRQZDV FDUULHGRXWDWq&IRUKRXUV$VDFRQWUROQDWXUDOVRUJKXPIHUPHQWDWLRQZLWKRXWDGGLWLRQRIDVWDUWHUFXOWXUHZDV carried out at 30q&IRUKRXUV'XULQJIHUPHQWDWLRQWLPHWKHDPRXQWRIEDFWHULDDFLGSURGXFLQJEDFWHULDFROLIRUP DQGSURWHRO\WLFEDFWHULDZHUHPRQLWRUHG7KHWLWUDWDEOHDFLGLW\S+VROXEOHSURWHLQDQGSURWHRO\WLFDFWLYLW\ZHUHDOVR PHDVXUHG$GGLWLRQRIL. plantarum6LQFUHDVHGVLJQL¿FDQWO\WKHLQLWLDOSRSXODWLRQRIWRWDOEDFWHULDODFWLFDFLG

bacteria and proteolytic bacteria to 10&)8PODQGVXSSUHVVHGWKHJURZWKRIFROLIRUPVLQGLFDWHGE\VLJLQL¿FDQWO\

GHFOLQH RI FROLIRUPV SRSXODWLRQ DIWHU K IHUPHQWDWLRQ7KH SURGXFWLRQ RI DFLG ZDV GRXEOHG RI WKDW LQ WKH QDWXUDO IHUPHQWDWLRQUHVXOWHGLQWKHORZHUS+WR%RWKQDWXUDOVRUJKXPIHUPHQWDWLRQDQGWKDWZLWKDGGLWLRQRISURWHRO\WLF

L. plantarum6VKRZHGVRPHSURWHRO\WLFDFWLYLWLHVGXULQJIHUPHQWDWLRQ

Keywords/DFWLFDFLGEDFWHULDVRUJKXPIHUPHQWDWLRQSURWHRO\WLFDFWLYLW\

INTRODUCTION

6RUJKXP Sorghum bicolor / 0RHQFK LV RQH RI WKH

PDMRUIRRGFURSVRIWKHVHPLDULGUHJLRQVRI$IULFDDQG$VLD Compared to other major cereal crops, sorghum has a distinct DGYDWDJHRIEHLQJGURXJKUHVLVWDQW,Q,QGRQHVLDHVSHFLDOO\ LQ WKH SURYLQFH RI (DVW 1XVD 7HQJJDUD IDUPHUV FXOWLYDWH VRUJKXPDVDVWDSOHIRRGFURSIRUFRQVXPSWLRQWKXVVRUJKXP LV DQ LPSRUWDQW VRXUFH RI HQHUJ\ SURWHLQ YLWDPLQV DQG PLQHUDOVIRUSHRSOHLQWKLVUHJLRQ

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'RXGXet al(ONKDOLIDet al'H0HVD

6WRQHVWUHHWet al 6RPH VWXGLHV KDYH VKRZQ WKDW

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DVRXUFHRIE. coliVSRUHIRUPLQJEDFWHULDVXFKDVB. cereus

and C. perfringens, and lactic acid bacteria. Traditionally,

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UHGXFLQJWKHLQFLGHQFHRI*UDPQHJDWLYHEDFWHULDDQGQRQ sporing bacterial pathogen (Kunene et al

In this study, Lactobacillus plantarum 6 ZKLFK

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6 RQ WKH SRSXODWLRQ RI VHOHFWHG EDFWHULD DQG FKHPLFDO FKDUDFWHULVWLFVGXULQJGXULQJIHUPHQWDWLRQ

MATERIALS AND METHODS Preparation of Sorghum Flour

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Sorghum Flour Fermentation

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plantarum 6 LQWR WKH PL[WXUH RI VRUJKXP ÀRXU ZLWK

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plantarum6

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Enumeration of Bacteria Population

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Chemical Analysis

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Proteolytic Activity Measurement

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RESULTS AND DISCUSSION

Bacterial Population During Sorghum Fermentation

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the supernatant could be produced by natural microorganisms LQ VRUJKXP ÀRXU DQG E\L. plantarum 6 2XU UHVXOWV

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Soluble Protein and Reducing Sugar Contents in Fermented Sorghum Flour

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Proteolytic producing microoorganisms in sorghum

and L. plantarum6SURGXFHGSURWHDVHVZKLFKGHJUDGH

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DPLQR DFLGV DQG VROXEOH SURWHLQ LQ IHUPHQWHG VRUJKXP

ZLWKLactobacillus brevis increased compared to the ones

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Lactobacillus fermentum. This results indicates that soluble

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Lactrobacillus plantarum 6

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Lactrobacillus plantarum 6

0

6L. plantarum

0

(6)

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Correira et alUHSRUWHGWKDWVRUJKXPIHUPHQWHG

ZLWKLactobacillus brevis had higher reducing sugar content

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simultaneously. Proteolytic attack to the proteins could make starch more accessible to bacterial amylases resulted in higher UHGXFLQJVXJDUFRQWHQW(ONKDOLIDet alVXJJHVWHGWKDW

VWDUFKJUDQXOHVLQXQIHUPHQWHGVRUJKXPÀRXUVDUHFRPSOHWHO\ HQFORVHGLQDYHU\FRPSDFWSURWHLQPDWUL[7KHSURWHLQEDUULHU VXUURXQGLQJWKHVWUDFKJUDQXOHPD\UHGXFHWKHK\GURO\VLVRI QDWLYHVWDUFKE\DP\ORO\WLFHQ]\PH7KHSUHVHQWRISURWHDVHV GHJUDGHVWKHSURWHLQPDWUL[DQGHQKDQFHGVWDUFKK\GURO\VLV E\LQFUHDVLQJVXUIDFHDUHDDQGHQDEOLQJVWDUFKWRLQWHUDFWZLWK amylases, thus increased the reducing sugar content.

CONCLUSIONS

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++ Characterization and Distribution of

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Three Shorgum Varieties in Sudan. Current Research in

%DFWHULRORJ\$VLDQQHWZRUN)RU6FLHQWL¿F,QIRUPDWLRQ ,661

$QJDOL&6RUJKXPFDUERK\GUDWHVDUHYLHZPlant

Foods for Human Nutrition

$QRQLP . 2I¿FLDO 0HWKRGV RI$QDO\VLV th Edition.

$VVRFLDWLRQ RI 2I¿FLDO $QDO\WLFDO &KHPLVWV Washington, DC.

%UXQR 0$ /D]]D &0 (UUDVWL 0$ /RSH] /0, &DI¿QL 12 DQG 3DUGR 0) 0LON FORWWLQJ DQGSURWHRO\WLFDFWLYLW\RIDQHQ]\PHSUHSDUDWLRQIURP

Bromelia hieronymiIUXLWVJournal of Food Science and

Technology

&RUUHLD , 1XQHV $ 'XDUWH ,) %DUURV $6 DQG Delgadillo, I. 6RUJKXPIHUPHQWDWLRQIROORZHG by spectroscopic techniques. Food Chemistry90

859.

&RUULHLD , 1XQHV $ *XHGHV 6 %DUURV $6 DQG Delgadillo, I. 6FUHHQLQJRIODFWLFDFLGEDFWHULD SRWHQWLDOO\XVHIXOIRUVRUJKXPIHUPHQWDWLRQ Journal of Cereal Science

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of Food Science7555

'XRGX.*%HOWRQ367\ORU-51DQG+DPDNHU%5 )DFWRUDIIHFWLQJVRUJKXPSURWHLQGLJHVWDELOLW\

Journal of Cereal Science

(ONKDOLID$26FKLIÀHU%DQG%HUQKDUGW5(IIHFW RIIHUPHQWDWLRQRQWKHIXQFWLRQDOSURSHUWLHVRIVRUJKXP

ÀRXUFood Chemistry

(ONKDOLID $2 %HUQKDUGW 5 %RQRPL ) ,DPHWWL 6 3DJDQL 0$ DQG =DUGL 0 )HUPHQWDWLRQ PRGL¿HVSURWHLQSURWHLQDQGSURWHLQVWDUFKLQWHUDFWLRQV in sorghum dough. European Food Research and

Technology

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Chemistry

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Flour Fiermented Spontenously and by Lactic Acid

Bacteria.0DVWHU7KHVLV)RRG6FLHQFHDQG7HFKQRORJ\

6WXG\3URJUDP*DGMDK0DGD8QLYHUVLW\<RJ\DNDUWD Indonesia.

.XQHQH1)+DVWLQJV-:DQG+RO\$%DFWHULDO SRSXODWLRQDVVRFLDWHGZLWKDVRUJKXPEDVHGIHUPHQWHG ZHDQLQJ FHUHDO International Journal of Food

Microbiology 49

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Enviromental Microbiology57

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International Journal of Food Microbiology

199.

0XJXOD -. 6RUKDXJ 7 DQG 6WHSDQLDN / E 3URWHRO\WLFDFWLYLWLHVLQ7RJZDD7DQ]DQLDQIHUPHQWHG IRRGInternational Journal of Food Microbiology:

0X\DQMD&0%.1DUYKXV-$7UHLPR-DQG/DQJVXUG 7,VRODWLRQFKDUDFWHUL]DWLRQDQGLGHQWL¿FDWLRQ

RI ODFWLF DFLG EDFWHULD IURP bushera: a Ugandan

WUDGLWLRQDO IHUPHQWHG EHYHUDJHInternational Journal

of Food Microbiology

1DEUGDOLN0*UDWD.DQG/DWDOD$3URWHRO\WLF

FFWLYLW\ RI Bacillus cereus strains. Proceeding of

ECOpole4

5D\ % Fundamental Food Microbiology. CRC

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