LAPORAN HASIL
PENELITIAN DISERTASI DOKTOR
TAHUN ANGGARAN 2011
Judul : Studi Ikatan Konden Tannin Dengan Lisin/Metionin Ditinjau Dari Profil Spektranya Serta Pengaruhnya Dalam Pakan Lengkap Ternak
Kambing Perah Terhadap Produksi Gas In Vitro Peneliti : Ir. Mashudi, M.Agr.Sc.
Dibiayai Oleh Direktorat Jendral Pendidikan Tinggi, Kementerian Pendidikan Nasional Melalui DIPA Universitas Brawijaya REV. 1 No. 0636/023-04.2.16/15/2011/R, Tanggal
30 Maret 2011, Dan Surat Keputusan Rektor Universitas Brawijaya Nomor: 214/SK/2011 Tanggal 2 Mei 2011
Universitas Brawijaya
Malang
2011
RINGKASAN
Penelitian ini secara umum bertujuan ingin mengkaji suplemen unggul berupa produk ”asam amino Lisin dan Metionin terproteksi dengan KT” terhadap produktifitas ternak perah yang sedang laktasi dengan menggunakan metoda laboratoris yaitu produksi gas in vitro. Produk ini bila diberikan ke ternak perah diharapkan dapat tersalurkan ke dalam pasca rumen untuk dicerna dan diserap yang pada gilirannya dapat meningkatkan prestasi produksi ternak perah baik produksi maupun kualitas.
Untuk mencapai tujuan tersebut telah dilakukan penelitian dalam dua tahap. Tahap I bertujuan mengetahui adanya ikatan secara kimia antara Lisin/Metionin dengan KT (Lisin/Metionin-KT komplek) pada larutan netral (pH= 7) dengan menggunakan uji Spektrofotometer UV Vis serta mengetahui adanya pelepasan ikatan komplek tersebut pada larutan asam (pH:2-3) dengan mengunakan uji Spektrofotometer UV Vis. Selanjutnya tahap II bertujuan mengetahui kualitas suplemen berupa produk ”asam amino Lisin dan Metionin terproteksi dengan KT” pada Pakan Lengkap ternak kambing perah dengan uji laboratoris (produksi gas, konsentrasi NH3 , nilai VFA dan nilai KCBO, NE, ME dan MP).
Metode penelitian tahap I dilakukan dengan metode percobaan kualitatif menggunakan 2 bahan asam amino dan campuran keduanya yaitu lisin/ metionin dan lisin+metionin dicampur dengan KT selanjutnya diamati perlindungan asam amino ini dengan melihat ikatannya dalam bentuk profil spektra (uji kualitatif) dengan mengunakan alat spektrophotometer UV Vis. Besarnya ikatan yaitu besarnya absorbansi diuji statistik dengan metode percobaan RAK. Untuk kontrol dilakukan pengamatan lisin/metionin dan lisin+metionin saja tanpa KT. Kombinasi antara lisin dan metionin sesuai dengan pedoman NRC (2001) yaitu perbandingan antara lisin dan metionin berkisar sebesar 3 : 1. Sedangkan level KT sesuai petunjuk dengan Barry and Forss (1983) bahwa perbandingan antara protein dan KT sebesar 11 : 1 sampai 16 : 1 (sehingga level KT terhadap asam amino berkisar antara 6% sampai 10%). Setiap bahan mengalami empat (4) level perlakuan proteksi dengan menggunakan KT dari ekstrak Mimosa. Sebagai ulangan (kelompok) digunakan inokulum cairan rumen.
Metode penelitian tahap II dilakukan untuk mengetahui pengaruh suplemen lisin, metionin terproteksi dengan KT dalam Pakan Lengkap kambing perah terhadap nilai-nilai parameter laboratoris seperti produksi gas, nilai VFA dan produksi NH3, dan prediksi ME, NE, OMD dan MP. Kandungan nutrisi standard Pakan Lengkap mengacu untuk kebutuhan kambing perah yang sedang laktasi (NRC, 2007). Penelitian ini dilakukan dengan metode percobaan dengan menggunakan rancangan Acak Kelompok, yang terdiri 17 perlakuan.
Hasil penelitian tahap I menunjukkan bahwa rofil spektra berupa λmax antara campuran
antara lisin/metionin (dan lisin+metionin) dengan berbagai level KT pada larutan pH netral memilki λmax lebih rendah (279 nm) dibanding λmax KT (288 nm), hal ini menunjukkan bahwa
λmax campuran dua zat bergeser ke kiri yang berarti telah terjadi ikatan hidrogen antara asam
amino tersebut dengan KT. Profil spektra berupa λmax antara campuran antara lisin/metionin
(dan lisin+metionin) dengan berbagai level KT pada larutan asam memilki λmax sama (279 nm)
dengan λmax KT (279 nm), hal ini menunjukkan bahwa λmax campuran dua zat tidak terjadi
pergeseran yang berarti tidak terjadi ikatan hidrogen antara asam amino tersebut dengan KT. Baik pada larutan netral maupun asam , semakin tinggi level KT semakin tinggi nilai absorbansinya (P<0,01) yang bermakna bahwa kerapatan elektron dua campuran senyawa ini semakin besar dan ikatan hidrogen antara asam amino dengan KT semakin kuat.
Hasil penelitian tahp II menunjukkan bahwa suplemen lsin-metionin teproteksi dengan KT secara umum menunjukkan bahwa KT berpengaruh sangat nyata (P<0,01) sedangkan level lisin-metionin tidak berbeda nyata (P>0,05) ditinjau dari parameter produksi gas, serta berpengaruh nyata (P<0,05) terhadap parameter b dan a+b, namun tidak berpengaruh nyata (P>0,05) terhadap parameter a dan c baik pengaruh KT maupun level lisin-metionin. Untuk
parameter lain menunjukkan bahwa suplemen lisin-metionin tidak berpengaruh nyata (P>0,05) terhadap KCBKdan KCBO, produksi VFA baik partial maupun total, prediksi ME, NE, OMD dan MP baik pengauh KT maupun pengaruh level Lisin-metionin, namun berpengaruh nyata (P<0,05) terhadap produksi amonia baik karena pengaruh KT maupun pengaruh level Lisinmetionin. Secara umum semakin tinggi level KT, semakin menurunkan produksi gas, parameter b dan c, kecernaan BK dan BO, NH3, VFA maupun prediksi ME, NE, MD dan MP. Selanjutnya semakin tinggi level Lisin-metionin, semakin meningkatkan produksi gas, parameter b dan c, kecernaan BK dan BO, NH3, dan VFA.
Dengan demikian dapat disimpulkan bahwa asam amino lisin/metionin dapat membentuk ikatan pada kondisi basa dan ikatan tersebut akan lepas pada kondisi asam. Dengan uji produksi gas in vitro, KT ternyata dapat menurunkan produksi gas dengan parameternya seperti besarnya degradasi, laju degradasi, serta dapat menurunkan kecernaan serta NH3. Sebaliknya Lisin dan metionin sendiri ternyata dapat meningkatkan produksi gas, dengan parameternya seperti besarnya degradasi, laju degradasi, serta dapat meningkatkan kecernaan, NH3 serta VFA. Dengan demikian KT dapat digunakan untuk melindungi asam amino pembatas lisin dan metionin dari degradasi mikroba rumen, sehinga suplemen lisin dan metionin terproteksi apabila diberikan ke ternak kambing perah diharapkan akan dapat meningkatkan produktifitasnya.
Kata kunci : Lisin, metionin, konden tanin (KT), profil spktra, absorbansi, produksi gas, amonia, VFA.
SUMMARY
The main objective of this research was to obtain such an excellent product, namely “Protected Lysine/Methionine”, and to know its effect on productivity of lactating of dairy goat using in vitro gas production method. This product is hopely able to be by-pass into pasca rumen, then digested and absorbed which finally can enhance performance of dairy goat, either its milk yield or milk quality.
The experiment was carried out through two stages. First, it was aimed to investigate whether there is chemical bound between Lysine/Methionine and Condensed Tannin (Lysine/Methionin-CT complex) at neutral solution (pH=7) as well as to investigate releasing of the complex at acid solution (pH=2-3) using Spectrophotometer UV Vis. Experiment method used in this stage was Qualitative experiment by using two amino acids and its mixture, while Lysine/Methionine mixed with Condensed Tannin and their bound was observed in form of spectra profile (qualitative) using spektrophotometer UV Vis equipment. The strength of the bond was tested on the absorption value statistically analysed using Randomized Block Design. The proportion of Lysine and Methionine was 3:1 (NRC, 2004), whereas CT level referred to Barry and Forss (1983) that proportion of protein and CT should be around 11:1 until 16:1 (so that CT level on amino acids around 6% until 10%). Each treatment consists of 4 level protections using CT taken from Mimosa Bark Extract and rumen liquid was used as replication. The second stage was to know the quality of product “Protected Lysine/Methionine” in complete feed of lactating dairy goat tested with in vitro gas production (Makkar, 1995). Measured variables were in vitro gas production, ammonia and VFA production and estimated OMD, NE, ME dan MP value, Nutrient Requirement of Lactating Dairy Goat (NRC, 2007) was used as
guidance for nutrient content of complete feed (treatment). Method applied in this stage was Randomized Block Design with 17 treatments.
The result of the first experiment showed that the spectra profile of λmax between the
mixture of Lysine and Methionine on various levels of CT in neutral pH had the lower λmax (279
nm), compared to λmax of CT (288 nm). This indicated that λmax of the mixture swings to the left
which was meant that there had already occured hidrogen bound between the amino acids and CT itself. While in acid solution, the spectra profile of λmax between the mixture of Lysine and
Methionine on various levels of CT had the same λmax (279 nm) as CT (279 nm). This indicated
that there was no swinging, meant that there was no hidrogen bound between the amino acids and CT itself. Either in neutral or acid solution, the higher of CT level, the higher of absorbancy value (P<0,01). In other say that the greater of the electron density of the two mixed compunds, the stronger of hidrogen bound between amino acids and CT.
The second experiment showed the result that in general the supplementary of Lysine and Methionine protected with CT explained the influence of CT level was very significantly different (P<0.01), while Lysine and Methionine levels were not significantly different (P>0.05) viewed from gas production. The influence of both CT level and Lysine and Methionine level was significantly different (P<0.05) upon parameter b and a+b, but no significant difference (P>0,05) on DMD, OMD. VFA production in total or partial, as well as on estimated ME, NE, OMD and MP. Ammonia production was influenced significantly (P<0.05) by both CT level and Lysine and Methionine level. There was tendency that the higher of CT level led to the lower of gas production, parameters b and c, DMD and OMD, NH3. VFA, as well as of estimated ME, NE, OMD and MP. Furthermore, the higher of Lysine and Methionine level led to the higher of gas production, parameters b and c, DMD and OMD, NH3. VFA.
In conclusion, Lysine and Methionine were able to create a bound in bases condition and the bound was released in acid condition. By in vitro gas production test, CT in fact could lower gas production and its parameters such as degradability rate, as well as digestibility and NH3. Meanwhile Lysine and Methionine could increase gas production and its parameters, digestibility, NH3 and VFA. Thus, CT could be applied to protect limiting amino acids such as Lysine and methionine from being degraded by ruminal microbes which is in turn the CT- protected Lysine and methionine supplemented to goat could improve their productivity Key Words : Lysine, methionine, condensed tannin (CT), spectra profile, absorbance, in-vitro gas production, ammonia, VFA.
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