Uji Aktivitas Diuretik Ekstrak N-Heksan Herba Pecut Kuda (Stachytarpheta jamaicensis L. Vahl) Pada Tikus

(1)

(2)

Lampiran 2.

Tumbuhan Pecut Kuda

(3)

Lampiran 3.

Hasil Mikroskopik dengan Perbesaran 10 × 40

Keterangan:

1. Stomata tipe diasitik

2. Pembuluh kayu dengan penebalan jala

3. Serat sklerenkim

(4)

Lampiran 4.

Perhitungan Hasil

Karakterisasi Simplisia dan Ekstrak n-Heksan Pecut

Kuda

Perhitungan Hasil Karakterisasi Simplisia Pecut Kuda

1. Perhitungan Penetapan Kadar Air

% Kadar Air =

volumeair (ml )

beratsampel (g)

x 100%

a.

Berat sampel = 5,011 g

Volume air = 0,4 ml

% Kadar Air =

0,4 ml

5,011 g

x 100% = 7,98 %

b.

Berat sampel = 5,012 g

Volume air = 0,5 ml

% Kadar Air =

0,5 ml

5,012 g

x 100% = 9,97 %

c.

Berat sampel = 5,004 g

Volume air = 0,4 ml

% Kadar Air =

0,4 ml

5,004 g

x 100% = 7,99 %

% Kadar Air Rata-Rata =

7,98%+9,97%+7,99%

3

= 6,65%

2. Perhitungan Penetapan Kadar Sari Larut dalam Air

% Kadar sari larut dalam air =

berat sari (g) berat sampel (g)

x

100

20

×

100%

a.

Berat sampel = 5,0193 g

Berat sari = 0,2042g

% Kadar sari larut dalam air =

0,2042 g 5,0193 g

x

100

20

×

100% = 20,34%

b.

Berat sampel = 5,0190 g

(5)

% Kadar sari larut dalam air =

0,2021 g

% Kadar sari larut dalam air rata-rata =

20,34%+20,13%+20,02%

3

= 20,16%

3. Perhitungan Penetapan Kadar Sari Larut dalam Etanol

% Kadar sari larut dalam etanol =

berat sari (g)

Beratsari = 0,294g

% Kadar sari larut dalam etanol =

0,294 g

% Kadar sari larut dalam etanol rata-rata =

28,34%+29,34%+27,25%

3

=

28,31%

4. Perhitungan Penetapan Kadar Abu Total

% Kadar abu total =

berat abu (g)

(6)

a.

Berat sampel = 3,06 g

Berat abu = 0,16g

% Kadar abu total =

0,16 g

3,06 g

×

100% = 5,22%

b.

Berat sampel = 3,08 g

Beratabu = 0,18g

% Kadar abu total =

0,18 g

3,08 g

×

100% = 5,84%

c.

Berat sampel = 3,04 g

Berat abu = 0,16g

% Kadar abu total =

0,16 g

3,04 g

×

100% = 5,26%

% Kadar abu total rata-rata =

5,22%+5,84%+5,26%

3

= 5,44%

5. Perhitungan Penetapan Kadar Abu Tidak Larut Asam

% Kadar abu tidak larut asam =

berat abu (g)

berat sampel (g)

×

100%

a.

Berat sampel = 3,06 g

Berat abu = 0,01g

% Kadar abu tidak larut asam =

0,01 g

3,06 g

×

100% = 0,32%

b.

Berat sampel = 3,08 g

Beratabu = 0,02g

% Kadar abu tidak larut asam =

0,02 g

3,08 g

×

100% = 0,64%

c.

Berat sampel = 3,04 g

Berat abu = 0,01g

% Kadar abu tidak larut asam =

0,01 g

(7)

% Kadar abu tidak larut asam rata-rata =

0,32%+0,64%+0,52%

3

= 0,42%

Perhitungan Hasil Karakterisasi Ekstrak n-Heksan Pecut Kuda

1. Perhitungan Penetapan Kadar Air

% Kadar Air =

volumeair (ml )

beratsampel (g)

x 100%

Berat sampel = 1,002 g

Volume air = 0,05 ml

% Kadar Air =

0,05 ml

1,002 g

x 100% = 4,99 %

2. Perhitungan Penetapan Kadar Abu Total

% Kadar abu total =

berat abu (g)

berat sampel (g)

×

100%

Berat sampel = 1,005 g

Berat abu = 0,015g

% Kadar abu total =

0,015 g

1,005 g

×

100% = 1,49%

3. Perhitungan Penetapan Kadar Abu Tidak Larut Asam

% Kadar abu tidak larut asam =

berat abu (g)

berat sampel (g)

×

100%

Berat sampel = 1,005 g

Berat abu = 0,004g

% Kadar abu tidak larut asam =

0,004 g

(8)

(9)

Lampiran 6.

Alat yang Digunakan

Modifikasi alat penampung urin

(10)

Lampiran 7.

Hasil Pengujian Dari AAS

Kelompok

Dosis

Absorbansi

Natrium

Kalium

Na CMC 0.5%

0.2424

0.1843

0.2527

0.1297

0.2099

0.1521

0.2729

0.1048

0.2328

0.0879

Furosemid 10

mg/kg bb

0.5896

0.3425

0.6097

0.3196

0.5032

0.3063

0.5568

0.2821

0.6302

0.2941

EnHPK 100

mg/kg bb

0.1898

0.1590

0.1702

0.1475

0.1346

0.1544

0.1046

0.1388

0.1236

EnHPK 150

mg/kg bb

0.3924

0.1966

0.5097

0.2792

0.3481

0.2469

0.4268

0.2206

0.5424

0.1596

EnHPK 200

mg/kg bb

0.3725

0.1348

0.4817

0.1853

0.4559

0.2045

0.2730

0.1253

(11)

Lampiran 8.

Data Volume Urin Tiap Jam (ml), Volume Total Urin Rata-rata

(ml), Kadar Natrium (meq/l), dan Kadar Kalium (meq/l)

Data Volume Urin Tiap Jam

Kelompok Pengujian Rata-rata volume urin tiap jam (ml)

1 2 3 4 5

Data Volume Total Urin Rata-rata, Kadar Natrium dan Kadar Kalium

No Parameter Kontrol Na-CMC 0,5%

Furosemid 10 mg/kg bb

Ekstrak n-heksan Pecut Kuda

Dosis 100

Volume Total Urin Rata-rata (ml)

1,1 3,3 1,85

Kadar Natrium (meq/l)

(12)

Lampiran 9.

Contoh perhitungan kadar elektrolit

Kadar Na =

Konsentrasi (mcg /ml )

Volume sampel

x 2500

Pembacaan pada AAS = 0,3988 mcg/ml

Kadar Na =

0,3988 mcg /ml

1 ml

x 2500 = 997 mcg/ml

= 0,997 mg/ml

meq/L =

Kadar Na (mg /ml )

BM Na

x 1 meq

=

0,997 mg /ml

(13)

Lampiran 10.

Data Kalibrasi Natrium

Kalibrasi Natrium

Konsentrasi

Absorbansi

0.0000

-0.0002

Penentuan Persamaan Regresi

Konsentrasi

Kurva Kalibrasi Natrium

(14)

a

=

∑ �� −

��

55–37.5

�

[1.08956468

−

0.739206 ]

=

2.4758

�

[17.5][0.350359 ]

=

2.4758

√

6.131277

=

2.4758

2.4761

(15)

Lampiran 11.

Hasil Kalibrasi Kalium

Kalibrasi Kalium

Konsentrasi

Absorbansi

0.0000

0.0003

Penentuan Persamaan Regresi

Konsentrasi

10.0000

_0.4379

_4.379

₁₀₀

_0.19175641

ΣX= 30 ΣY=1.2924

Kurva Kalibrasi Kalium

(16)

A

=

∑ �� −

�

[220

−

150][0.41262862

−

0.278383 ]

=

3.0638

�

[70][0.134246 ]

=

3.0638

√

9.397196

=

3.0638

3.0654

(17)

Lampiran 12.

Hasil data statistik ANAVA dengan Uji Tukey

Descriptives

Volume Urin Jam ke-1

N Mean Std.

Deviation Std. Error

95% Confidence Interval for Mean

Min Max Furosemid 10 mg/kg

bb 5 .5400 .53080 .23738 -.1191 1.1991 .00 1.20

Tukey HSD

95% Confidence Interval

Lower Bound

Upper Bound

Na-CMC 0,5% Furosemid 10

mg/kg bb -.36000 .26754 .667 -1.1606 .4406 Furosemid 10 mg/kg

(18)

Deskriptive

N Mean Std. Deviation

Std. Error

Min Max Furosemid 10

mg/kg bb 5 1.1000 1.08570 .48554 -.2481 2.4481 .00 2.35 EnHPK 100 mg/kg

bb 5 .4900 .46152 .20640 -.0831 1.0631 .00 1.00 EnHPK 150 mg/kg

bb 5 1.0100 .86342 .38613 -.0621 2.0821 .00 2.00 EnHPK 200 mg/kg

bb 5 .5600 .56833 .25417 -.1457 1.2657 .00 1.30

Total 25 .7040 .73625 .14725 .4001 1.0079 .00 2.35

Multiple Comparisons

Tukey HSD

(I) Kelompok Pengujian (J) Kelompok Pengujian

Mean Difference (I-J)

Std. Error Sig.

Lower Bound

Upper Bound

(19)

Descriptives

N Mean Std. Deviation

Std. Error

Min Max

Furosemid 10

mg/kg bb 5 1.6900 .80808 .36139 .6866 2.6934 1.10 3.00

Volume Urin Jam ke-3 Tukey HSD

(I) Kelompok Pengujian (J)Kelompok

Pengujian

Std. Error Sig.

95% Confidence Interval Lower Bound

Upper Bound

(20)

Descriptives

N Mean Std.

Min Max Furosemid 10 mg/kg

bb 5 2.4300 .76616 .34264 1.4787 3.3813 1.60 3.45

(I) Kelompok Pengujian (J) Kelompok

Pengujian

Std. Error Sig.

mg/kg bb -1.28000

*_.36116 _.015 _-2.3607 _-.1993

EnHPK 200

mg/kg bb -.80000 .36116 .215 -1.8807 .2807

Furosemid 10 mg/kg bb Na-CMC 0,5% 1.59000* .36116 .002 .5093 2.6707

EnHPK 100

mg/kg bb 1.14000

*

(21)

Descriptives

N Mean Std.

Min Max

Furosemid 10

mg/kg bb 5 3.2500 .35707 .15969 2.8066 3.6934 2.80 3.70

EnHPK 100 mg/kg

bb 5 1.7800 .22528 .10075 1.5003 2.0597 1.50 2.10

EnHPK 150 mg/kg

bb 5 2.9600 .29665 .13266 2.5917 3.3283 2.50 3.30

EnHPK 200 mg/kg

bb 5 2.2700 .44102 .19723 1.7224 2.8176 1.80 2.90

Total

25 2.2780 .83617 .16723 1.9328 2.6232 1.00 3.70

Multiple Comparisons

(I) Kelompok Pengujian (J) Kelompok

Pengujian

Std. Error Sig.

95% Confidence Interval Lower

Bound

Upper Bound

mg/kg bb -2.12000

*

mg/kg bb -1.83000

*

.19499 .000 -2.4135 -1.2465

EnHPK 200

mg/kg bb -1.14000

*

.19499 .000 -1.7235 -.5565

Furosemid 10 mg/kg bb Na-CMC 0,5%

2.12000* .19499 .000 1.5365 2.7035

EnHPK 100

mg/kg bb 1.47000

*

(22)

Descriptives

Volume total urin

N

Mean

95% Confidence

Interval for Mean

Min

Max

Furosemid 10

mg/kgbb

5 3.2500 .35707 .15969 2.8066

3.6934

2.80

3.70 Multiple Comparisons

Volume total urin

Tukey HSD

(I) Kelompok uji

(J)

95% Confidence

Interval

Lower

Bound

Upper

Bound

CMC-Na 0.5%

Furosemid

10 mg/kgbb

Furosemid 10

mg/kgbb

(23)

Descriptives

Kadar Natrium

N

Mean

Std.

Deviation

Std.

Error

95% Confidence

Interval for Mean

Min

Max

Kadar Natrium Tukey HSD

(I) Kelompok uji

(J) Kelompok uji

Mean Difference

(I-J) Std. Error Sig.

Lower Bound

Upper Bound

CMC-Na 0.5% Furosemid 10 mg/kgbb

(24)

Descriptives

Kadar Kalium

N Mean Std.

Min Max

Furosemid 10

mg/kg bb 5 45.7472 3.42493 1.53167 41.4946 49.9998 41.82 50.67

Kadar Kalium Tukey HSD

(I)Kelompok Pengujian

(J)Kelompok Pengujian

Mean Difference

(I-J) Std. Error Sig.

Lower Bound

Upper Bound

Na-CMC 0,5% Furosemid 10 mg/kg

bb -25.94624

*

3.29545 .000 -35.8075 -16.0850

EnHPK 100 mg/kg

bb -.30756 3.29545 1.000 -10.1688 9.5537

EnHPK 150 mg/kg

bb -13.00827

*

3.29545 .006 -22.8695 -3.1471

EnHPK 200 mg/kg

bb -2.80904 3.29545 .911 -12.6703 7.0522

Furosemid 10 mg/kg bb Na-CMC 0,5% 25.94624* 3.29545 .000 16.0850 35.8075 EnHPK 100 mg/kg

bb 25.63868

*

3.29545 .000 15.7775 35.4999

EnHPK 150 mg/kg

bb 12.93797

*

3.29545 .007 3.0768 22.7992

EnHPK 200 mg/kg

bb 23.13720

*

3.29545 .000 13.2760 32.9984