BAB V SIMPULAN DAN SARAN

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60 BAB V

SIMPULAN DAN SARAN

5.1. Simpulan

1)

Senyawa asam 2-(3-klorobenzoiloksi)benzoat memiliki efek toksik

yang sama dengan asam asetilsalisilat.

2)

Pada pengamatan organ lambung : penghentian pemakaian

senyawa asam 2-(3-klorobenzoiloksi)benzoat memberikan hasil

efek perbaikan dari iritasi lambung dibanding senyawa asam

asetilsalisilat.

3)

Pada parameter urin : data toksisitas subkronis senyawa asam

2-(3-klorobenzoiloksi)benzoat tidak berbeda dengan data subkronis

asam asetilsalisilat.

4)

Pada parameter profil darah : data toksisitas subkronis senyawa

asam 2-(3-klorobenzoiloksi)benzoat antara mencit jantan dan

betina memberikan hasil yang berbeda.

5.2. Saran

Dilakukan penelitian lanjutan pada pemeriksaan histopatologi

organ atau penelitian khusus pada organ ginjal pada mencit jantan dan

betina dan dilakukan penelitian toksisitas subkronis pada hewan dengan

tingkat spesies yang lebih tinggi untuk pemeriksaan biokimia darah pada

pemberian senyawa asam 2-(3-klorobenzoiloksi)benzoat secara subkronis

untuk melengkapi kemungkinan efek samping yang ditimbulkan dari

pemakaian senyawa asam 2-(3-klorobenzoiloksi)benzoat secara subkronis.

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61 DAFTAR PUSTAKA

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65 LAMPIRAN A

Perkembangan Berat Badan Mencit Interval Tiap Minggu

Tabel L.1.1. Perkembangan berat badan (g) kelompok mencit jantan

Kelompok ♂ No. T0 T1 T2 T3 T4 T5 T6

Kontrol Negatif (PGA 3%) 17 15 19 19 25 24 18 16 20 24 24 25 19 18 23 24 24 21 20 18 22 26 30 23 Rata-rata ± SD 16,8 ± 1,5 21 ± 1,83 23,3 ± 2,99 26 ± 2,71 23 ± 1,71

Kontrol Positif (AAS 1,3 mg/20 gBB) 1 21 25 26 28 26 2 17 19 18 20 16 3 19 21 24 27 26 4 21 19 17 18 16 Rata-rata ± SD 19,5 ± 1,91 21 ± 2,83 21,3 ± 4,43 23 ± 4,99 21 ± 5,77 Asam 2-(3-klorobenzoiloksi)benzoat 1,3 mg/20 gBB 1 18 21 23 25 23 2 20 23 23 27 24 3 20 21 22 23 21 4 17 20 22 25 21 Rata-rata ± SD 18,75 ± 1,5 21 ± 1,26 22,5 ± 0,58 25 ± 1,63 23 ± 1,5 Asam 2-(3-klorobenzoiloksi)benzoat 2,6 mg/20 gBB 9 22 25 27 27 27 10 22 27 26 24 25 51 19 21 21 19 19 52 24 26 25 24 25 Rata-rata ± SD 21,8 ± 2,06 24 ± 2,63 24,8 ± 2,63 24 ± 3,32 24 ± 3,46

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66

Asam 2-(3-klorobenzoiloksi)benzoat 3,9 mg/20 gBB 17 16 17 17 19 17 18 20 21 25 27 23 19 19 20 23 22 21 20 20 22 20 27 24 Rata-rata ± SD 18,8 ± 1,89 20 ± 2,16 21,25 ± 3,5 24 ± 3,95 21 ± 3,1

Kontrol Positif (AAS 1,3 mg/20 gBB) Satelit 9 20 20 22 22 19 21 23 10 21 21 21 21 25 25 26 11 19 22 25 27 25 23 25 12 17 18 15 16 15 15 17 Rata-rata ± SD 17,25 ± 1,5 20 ± 1,71 20,8 ± 4,19 22 ± 4,51 21 ± 4,90 21 ± 4,32 23 ± 4,03 Asam 2-(3-klorobenzoiloksi)benzoat 1,3 mg/20 gBB Satelit 5 17 21 22 25 22 20 18 6 16 23 21 26 23 23 22 7 23 21 22 26 26 25 23 8 23 20 27 29 27 26 24 Rata-rata ± SD 19,8 ± 3,77 21 ± 1,26 23 ± 2,71 26,5 ± 1,73 25 ± 2,38 24 ± 2,65 22 ± 2,63 Asam 2-(3-klorobenzoiloksi)benzoat 2,6 mg/20 gBB Satelit 13 20 23 24 30 26 28 27 14 22 23 26 27 28 28 30 15 24 26 29 33 22 20 22 16 23 22 22 26 26 26 28 Rata-rata ± SD 22,5 ± 1,71 24 ± 1,73 25,3 ± 2,99 29 ± 3,16 26 ± 2,52 26 ± 3,79 26,8 ±3,4 Asam 2-(3-klorobenzoiloksi)benzoat 3,9 mg/20 gBB Satelit 21 19 19 20 25 22 24 30 22 19 17 20 23 24 25 25 23 14 24 26 29 25 28 27 24 18 20 23 26 21 23 26 Rata-rata ± SD 17,5 ± 2,38 20 ± 2,94 22,3 ± 2,87 25,8 ± 2,50 23 ± 1,73 25 ± 2,16 27 ± 2,16

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67 Tabel L.1.2. Perkembangan berat badan (g) kelompok mencit betina

Kelompok ♀ No. T0 T1 T2 T3 T4 T5 T6

Kontrol Negatif (PGA 3%) 47 20 20 22 20 18 48 22 22 21 24 22 49 22 22 19 23 23 50 17 17 19 22 22 Rata-rata ± SD 20,3 ± 2,36 20 ± 2,36 20,3 ± 1,5 22,3 ± 1,71 21 ± 2,22

Kontrol Positif (AAS 1,3 mg/20 gBB) 25 15 15 11 12 14 26 21 21 21 23 25 27 18 18 20 22 23 28 19 19 21 19 21 Rata-rata ± SD 18,3 ± 2,5 18 ± 2,5 18,3 ± 4,86 19 ± 4,97 21 ± 4,79 Asam 2-(3-klorobenzoiloksi)benzoat 1,3 mg/20 gBB 25 21 18 22 25 22 26 18 17 20 22 23 27 17 15 19 24 20 28 19 18 21 23 21 Rata-rata ± SD 18,8 ± 1,71 17 ± 1,41 20,5 ± 1,29 23,5 ± 1,29 21 ± 1,29 Asam 2-(3-klorobenzoiloksi)benzoat 2,6 mg/20 gBB 33 20 19 23 23 20 34 20 21 19 21 19 35 14 15 13 14 12 36 21 22 22 23 21 Rata-rata ± SD 18,8 ± 3,2 19,3 ±3,1 19,3 ± 4,5 20,3 ±4,27 18 ± 4,08

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68

Asam 2-(3-klorobenzoiloksi)benzoat 3,9 mg/20 gBB 41 19 21 23 22 21 42 20 22 25 24 23 43 16 19 22 25 20 44 19 21 22 25 23 Rata-rata ± SD 18,5 ± 1,73 21 ± 1,26 23 ± 1,41 24 ± 1,41 21,8 ±1,5

Kontrol Positif (AAS 1,3 mg/20 gBB) Satelit 9 20 20 22 22 19 21 23 10 21 21 21 21 25 25 26 11 19 22 25 27 25 23 25 12 17 18 15 16 15 15 17 Rata-rata ± SD 17,3 ± 1,5 17 ± 1,5 20,5 ± 3,11 19,8 ± 4,35 19 ± 2,63 19,5 ± 3 21 ± 2,38 Asam 2-(3-klorobenzoiloksi)benzoat 1,3 mg/20 gBB Satelit 5 17 21 22 25 22 20 18 6 16 23 21 26 23 23 22 7 23 21 22 26 26 25 23 8 23 20 27 29 27 26 24 Rata-rata ± SD 21,3 ± 2,36 23 ± 1,83 25 ± 2,16 24,5 ± 5,45 23 ± 4,97 24 ± 5,19 26 ± 4,03 Asam 2-(3-klorobenzoiloksi)benzoat 2,6 mg/20 gBB Satelit 13 20 23 24 30 26 28 27 14 22 23 26 27 28 28 30 15 24 26 29 33 22 20 22 16 23 22 22 26 26 26 28 Rata-rata ± SD 18,3 ± 3,14 20 ±2,1 6 21,8 ± 1,89 23 ± 3,65 21 ± 3,51 19,5 ±3,7 22 ± 4,24 Asam 2-(3-klorobenzoiloksi)benzoat 3,9 mg/20 gBB Satelit 21 19 19 20 25 22 24 30 22 19 17 20 23 22 25 25 23 14 24 26 29 25 28 27 24 18 20 23 26 21 23 26 Rata-rata ± SD 19,3 ± 3,14 20,5 ±3,1 21,8 ± 3,26 21,5 ± 3,53 23 ± 3,87 23 ± 3,16 25 ± 2,68

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69 LAMPIRAN B

Hasil Statistik Perkembangan Berat Badan Mencit pada Kelompok Uji

dan Satelit

Tabel L.2.1.

Olahan data perkembangan berat badan mencit jantan

Descriptives Minggu Pertama N Mean Std. Deviation Std. Error 95% Confidence Interval for Mean

Minimum Maximum Lower Bound Upper Bound K (-) 4 4.25 .500 .250 3.45 5.05 4 5 K (+) Uji 4 1.50 2.517 1.258 -2.50 5.50 -2 4 K (+) Satelit 4 1.00 1.414 .707 -1.25 3.25 0 3 Uji 1.3 mg/20 gBB 4 2.50 1.000 .500 .91 4.09 1 3 Uji 2.6 mg/20 gBB 4 3.00 1.414 .707 .75 5.25 2 5 Uji 3.9 mg/20 gBB 4 1.25 .500 .250 .45 2.05 1 2 Sat. 1.3 mg/20 gBB 4 1.50 4.796 2.398 -6.13 9.13 -3 7 Sat. 2.6 mg/20 gBB 4 1.25 1.708 .854 -1.47 3.97 -1 3

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70

Sat. 3.9 mg/20 gBB 4 2.50 5.260 2.630 -5.87 10.87 -2 10

Total 36 2.08 2.579 .430 1.21 2.96 -3 10

Test of Homogeneity of Variances

Levene Statistic df1 df2 Sig.

4.381 8 27 .002

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71

Descriptives Minggu Ke dua N Mean Std. Deviation Std. Error 95% Confidence Interval for Mean

Minimum Maxim um Lower Bound Upper Bound K (-) 4 2.25 2.062 1.031 -1.03 5.53 0 4 K (+) Uji 4 .25 2.217 1.109 -3.28 3.78 -2 3 K (+) Satelit 4 .50 2.646 1.323 -3.71 4.71 -3 3 Uji 1.3 mg/20 gBB 4 1.25 .957 .479 -.27 2.77 0 2 Uji 2.6 mg/20 gBB 4 .00 1.414 .707 -2.25 2.25 -1 2 Uji 3.9 mg/20 gBB 4 1.25 2.754 1.377 -3.13 5.63 -2 4 Sat. 1.3 mg/20 gBB 4 1.75 3.775 1.887 -4.26 7.76 -2 7 Sat. 2.6 mg/20 gBB 4 1.75 1.500 .750 -.64 4.14 0 3 Sat. 3.9 mg/20 gBB 4 2.25 .957 .479 .73 3.77 1 3 Total 36 1.25 2.103 .350 .54 1.96 -3 7

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72

1.664 8 27 .153

Data homogen

ANOVA

Sum of Squares df Mean Square F Sig.

Between Groups 22.500 8 2.813 .574 .790

Within Groups 132.250 27 4.898

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73

Descriptives Minggu Ke tiga N Mean Std. Deviation Std. Error 95% Confidence Interval for Mean

Minimum Maximum Lower Bound Upper Bound K (-) 4 2.50 3.000 1.500 -2.27 7.27 0 6 K (+) Uji 4 2.00 .816 .408 .70 3.30 1 3 K (+) Satelit 4 .75 .957 .479 -.77 2.27 0 2 Uji 1.3 mg/20 gBB 4 2.50 1.291 .645 .45 4.55 1 4 Uji 2.6 mg/20 gBB 4 -1.25 .957 .479 -2.77 .27 -2 0 Uji 3.9 mg/20 gBB 4 2.50 3.317 1.658 -2.78 7.78 -1 7 Sat. 1.3 mg/20 gBB 4 3.50 1.291 .645 1.45 5.55 2 5 Sat. 2.6 mg/20 gBB 4 3.75 2.062 1.031 .47 7.03 1 6 Sat. 3.9 mg/20 gBB 4 3.50 1.000 .500 1.91 5.09 3 5 Total 36 2.19 2.227 .371 1.44 2.95 -2 7

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74

2.187 8 27 .061

Data tidak homogen

Minggu Ke empat N Mean Std. Deviation Std. Error 95% Confidence Interval for Mean

Minimum Maximum Lower Bound Upper Bound K (-) 4 -2.50 3.416 1.708 -7.94 2.94 -7 1 K (+) Uji 4 -2.25 1.258 .629 -4.25 -.25 -4 -1 K (+) Satelit 4 -.50 3.109 1.555 -5.45 4.45 -3 4 Uji 1.3 mg/20 gBB 4 -2.75 .957 .479 -4.27 -1.23 -4 -2 Uji 2.6 mg/20 gBB 4 .50 .577 .289 -.42 1.42 0 1 Uji 3.9 mg/20 gBB 4 -2.50 1.291 .645 -4.55 -.45 -4 -1 Sat. 1.3 mg/20 gBB 4 -2.00 1.414 .707 -4.25 .25 -3 0

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75

Sat. 2.6 mg/20 gBB 4 -3.50 5.447 2.723 -12.17 5.17 -11 1

Sat. 3.9 mg/20 gBB 4 -2.75 2.630 1.315 -6.93 1.43 -5 1

Total 36 -2.03 2.635 .439 -2.92 -1.14 -11 4

2.483 8 27 .037

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76

Descriptives Minggu Kelima N Mean Std. Deviation Std. Error 95% Confidence Interval for Mean

Minimum Maximum Lower Bound Upper Bound K (+) Satelit 4 .00 1.633 .816 -2.60 2.60 -2 2 Sat. 1.3 mg/20 gBB 4 -1.00 .816 .408 -2.30 .30 -2 0 Sat. 2.6 mg/20 gBB 4 .00 1.633 .816 -2.60 2.60 -2 2 Sat. 3.9 mg/20 gBB 4 2.00 .816 .408 .70 3.30 1 3 Total 16 .25 1.612 .403 -.61 1.11 -2 3

.400 3 12 .756

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77

ANOVA

Sum of Squares Df Mean Square F Sig.

Between Groups 19.000 3 6.333 3.800 .040

Total 39.000 15

Multiple Comparisons

Tukey HSD

(I) Kelompok (J) Kelompok

Mean Difference

(I-J) Std. Error Sig.

90% Confidence Interval Lower Bound Upper Bound K (+) Satelit Sat. 1.3 mg/20 gBB 1.000 .913 .699 -1.34 3.34 Sat. 2.6 mg/20 gBB .000 .913 1.000 -2.34 2.34 Sat. 3.9 mg/20 gBB -2.000 .913 .181 -4.34 .34

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78

Sat. 1.3 mg/20 gBB K (+) Satelit -1.000 .913 .699 -3.34 1.34 Sat. 2.6 mg/20 gBB -1.000 .913 .699 -3.34 1.34 Sat. 3.9 mg/20 gBB -3.000* _.913 _.029 _-5.34 _-.66 Sat. 2.6 mg/20 gBB K (+) Satelit .000 .913 1.000 -2.34 2.34 Sat. 1.3 mg/20 gBB 1.000 .913 .699 -1.34 3.34 Sat. 3.9 mg/20 gBB -2.000 .913 .181 -4.34 .34 Sat. 3.9 mg/20 gBB K (+) Satelit 2.000 .913 .181 -.34 4.34 Sat. 1.3 mg/20 gBB 3.000* _.913 _.029 _.66 _5.34 Sat. 2.6 mg/20 gBB 2.000 .913 .181 -.34 4.34

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79

Descriptives Minggu Ke enam N Mean Std. Deviation Std. Error 95% Confidence Interval for Mean

Minimum Maximum Lower Bound Upper Bound K (+) Satelit 4 1.75 .500 .250 .95 2.55 1 2 Sat. 1.3 mg/20 gBB 4 -.75 1.893 .946 -3.76 2.26 -2 2 Sat. 2.6 mg/20 gBB 4 1.25 1.500 .750 -1.14 3.64 -1 2 Sat. 3.9 mg/20 gBB 4 .50 3.873 1.936 -5.66 6.66 -3 6 Total 16 .69 2.272 .568 -.52 1.90 -3 6

2.382 3 12 .121

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80

ANOVA

Between Groups 14.188 3 4.729 .897 .471

Total 77.438 15

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81 Tabel L.2.2.

Olahan data perkembangan berat badan mencit betina

Descriptives Minggu Pertama N Mean Std. Deviation Std. Error 95% Confidence Interval for Mean

Minimum Maximum Lower Bound Upper Bound K (-) 4 .00 .000 .000 .00 .00 0 0 K (+) Uji 4 .00 .000 .000 .00 .00 0 0 K (+) Satelit 4 1.00 1.414 .707 -1.25 3.25 0 3 Uji 1.3 mg/20 gBB 4 -1.50 .577 .289 -2.42 -.58 -2 -1 Uji 2.6 mg/20 gBB 4 .50 1.000 .500 -1.09 2.09 -1 1 Uji 3.9 mg/20 gBB 4 2.25 .500 .250 1.45 3.05 2 3 Sat. 1.3 mg/20 gBB 4 1.50 4.796 2.398 -6.13 9.13 -3 7 Sat. 2.6 mg/20 gBB 4 1.25 1.708 .854 -1.47 3.97 -1 3 Sat. 3.9 mg/20 gBB 4 2.50 5.260 2.630 -5.87 10.87 -2 10 Total 36 .83 2.513 .419 -.02 1.68 -3 10

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82

6.739 8 27 .000

Data tidak homogen

Descriptives Minggu Ke dua N Mean Std. Deviation Std. Error 95% Confidence Interval for Mean

Minimum Maximum Lower Bound Upper Bound K (-) 4 .00 2.449 1.225 -3.90 3.90 -3 2 K (+) Uji 4 .25 3.096 1.548 -4.68 5.18 -4 3 K (+) Satelit 4 .50 2.646 1.323 -3.71 4.71 -3 3 Uji 1.3 mg/20 gBB 4 3.50 .577 .289 2.58 4.42 3 4 Uji 2.6 mg/20 gBB 4 -.25 3.096 1.548 -5.18 4.68 -3 4

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83

Uji 3.9 mg/20 gBB 4 2.25 .957 .479 .73 3.77 1 3 Sat. 1.3 mg/20 gBB 4 1.75 3.775 1.887 -4.26 7.76 -2 7 Sat. 2.6 mg/20 gBB 4 1.75 1.500 .750 -.64 4.14 0 3 Sat. 3.9 mg/20 gBB 4 2.25 .957 .479 .73 3.77 1 3 Total 36 1.33 2.414 .402 .52 2.15 -4 7

1.657 8 27 .155

Data homogen

ANOVA

Between Groups 51.500 8 6.438 1.140 .370

Total 204.000 35

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84

Descriptives Minggu Ke tiga N Mean Std. Deviation Std. Error 95% Confidence Interval for Mean

Minimum Maximum Lower Bound Upper Bound K (-) 4 2.00 2.708 1.354 -2.31 6.31 -2 4 K (+) Uji 4 .75 1.893 .946 -2.26 3.76 -2 2 K (+) Satelit 4 .75 .957 .479 -.77 2.27 0 2 Uji 1.3 mg/20 gBB 4 3.00 1.414 .707 .75 5.25 2 5 Uji 2.6 mg/20 gBB 4 1.00 .816 .408 -.30 2.30 0 2 Uji 3.9 mg/20 gBB 4 1.00 2.309 1.155 -2.67 4.67 -1 3 Sat. 1.3 mg/20 gBB 4 3.50 1.291 .645 1.45 5.55 2 5 Sat. 2.6 mg/20 gBB 4 3.75 2.062 1.031 .47 7.03 1 6 Sat. 3.9 mg/20 gBB 4 3.50 1.000 .500 1.91 5.09 3 5 Total 36 2.14 1.959 .326 1.48 2.80 -2 6

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85

1.593 8 27 .173

Data homogen

ANOVA

Between Groups 54.056 8 6.757 2.273 .053

Total 134.306 35

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86

Multiple Comparisons

Tukey HSD

(I) Kelompok (J) Kelompok

Mean Difference (I-J) Std. Error Sig. 90% Confidence Interval Lower Bound Upper Bound K (-) K (+) Uji 1.250 1.219 .980 -2.46 4.96 K (+) Satelit 1.250 1.219 .980 -2.46 4.96 Uji 1.3 mg/20 gBB -1.000 1.219 .995 -4.71 2.71 Uji 2.6 mg/20 gBB 1.000 1.219 .995 -2.71 4.71 Uji 3.9 mg/20 gBB 1.000 1.219 .995 -2.71 4.71 Sat. 1.3 mg/20 gBB -1.500 1.219 .942 -5.21 2.21 Sat. 2.6 mg/20 gBB -1.750 1.219 .874 -5.46 1.96 Sat. 3.9 mg/20 gBB -1.500 1.219 .942 -5.21 2.21 K (+) Uji K (-) -1.250 1.219 .980 -4.96 2.46 K (+) Satelit .000 1.219 1.000 -3.71 3.71

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87

Uji 1.3 mg/20 gBB -2.250 1.219 .654 -5.96 1.46 Uji 2.6 mg/20 gBB -.250 1.219 1.000 -3.96 3.46 Uji 3.9 mg/20 gBB -.250 1.219 1.000 -3.96 3.46 Sat. 1.3 mg/20 gBB -2.750 1.219 .401 -6.46 .96 Sat. 2.6 mg/20 gBB -3.000 1.219 .294 -6.71 .71 Sat. 3.9 mg/20 gBB -2.750 1.219 .401 -6.46 .96 K (+) Satelit K (-) -1.250 1.219 .980 -4.96 2.46 K (+) Uji .000 1.219 1.000 -3.71 3.71 Uji 1.3 mg/20 gBB -2.250 1.219 .654 -5.96 1.46 Uji 2.6 mg/20 gBB -.250 1.219 1.000 -3.96 3.46 Uji 3.9 mg/20 gBB -.250 1.219 1.000 -3.96 3.46 Sat. 1.3 mg/20 gBB -2.750 1.219 .401 -6.46 .96 Sat. 2.6 mg/20 gBB -3.000 1.219 .294 -6.71 .71 Sat. 3.9 mg/20 gBB -2.750 1.219 .401 -6.46 .96 Uji 1.3 mg/20 gBB K (-) 1.000 1.219 .995 -2.71 4.71

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88

K (+) Uji 2.250 1.219 .654 -1.46 5.96 K (+) Satelit 2.250 1.219 .654 -1.46 5.96 Uji 2.6 mg/20 gBB 2.000 1.219 .775 -1.71 5.71 Uji 3.9 mg/20 gBB 2.000 1.219 .775 -1.71 5.71 Sat. 1.3 mg/20 gBB -.500 1.219 1.000 -4.21 3.21 Sat. 2.6 mg/20 gBB -.750 1.219 .999 -4.46 2.96 Sat. 3.9 mg/20 gBB -.500 1.219 1.000 -4.21 3.21 Uji 2.6 mg/20 gBB K (-) -1.000 1.219 .995 -4.71 2.71 K (+) Uji .250 1.219 1.000 -3.46 3.96 K (+) Satelit .250 1.219 1.000 -3.46 3.96 Uji 1.3 mg/20 gBB -2.000 1.219 .775 -5.71 1.71 Uji 3.9 mg/20 gBB .000 1.219 1.000 -3.71 3.71 Sat. 1.3 mg/20 gBB -2.500 1.219 .524 -6.21 1.21 Sat. 2.6 mg/20 gBB -2.750 1.219 .401 -6.46 .96 Sat. 3.9 mg/20 gBB -2.500 1.219 .524 -6.21 1.21

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89

Uji 3.9 mg/20 gBB K (-) -1.000 1.219 .995 -4.71 2.71 K (+) Uji .250 1.219 1.000 -3.46 3.96 K (+) Satelit .250 1.219 1.000 -3.46 3.96 Uji 1.3 mg/20 gBB -2.000 1.219 .775 -5.71 1.71 Uji 2.6 mg/20 gBB .000 1.219 1.000 -3.71 3.71 Sat. 1.3 mg/20 gBB -2.500 1.219 .524 -6.21 1.21 Sat. 2.6 mg/20 gBB -2.750 1.219 .401 -6.46 .96 Sat. 3.9 mg/20 gBB -2.500 1.219 .524 -6.21 1.21 Sat. 1.3 mg/20 gBB K (-) 1.500 1.219 .942 -2.21 5.21 K (+) Uji 2.750 1.219 .401 -.96 6.46 K (+) Satelit 2.750 1.219 .401 -.96 6.46 Uji 1.3 mg/20 gBB .500 1.219 1.000 -3.21 4.21 Uji 2.6 mg/20 gBB 2.500 1.219 .524 -1.21 6.21 Uji 3.9 mg/20 gBB 2.500 1.219 .524 -1.21 6.21 Sat. 2.6 mg/20 gBB -.250 1.219 1.000 -3.96 3.46

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90

Sat. 3.9 mg/20 gBB .000 1.219 1.000 -3.71 3.71 Sat. 2.6 mg/20 gBB K (-) 1.750 1.219 .874 -1.96 5.46 K (+) Uji 3.000 1.219 .294 -.71 6.71 K (+) Satelit 3.000 1.219 .294 -.71 6.71 Uji 1.3 mg/20 gBB .750 1.219 .999 -2.96 4.46 Uji 2.6 mg/20 gBB 2.750 1.219 .401 -.96 6.46 Uji 3.9 mg/20 gBB 2.750 1.219 .401 -.96 6.46 Sat. 1.3 mg/20 gBB .250 1.219 1.000 -3.46 3.96 Sat. 3.9 mg/20 gBB .250 1.219 1.000 -3.46 3.96 Sat. 3.9 mg/20 gBB K (-) 1.500 1.219 .942 -2.21 5.21 K (+) Uji 2.750 1.219 .401 -.96 6.46 K (+) Satelit 2.750 1.219 .401 -.96 6.46 Uji 1.3 mg/20 gBB .500 1.219 1.000 -3.21 4.21 Uji 2.6 mg/20 gBB 2.500 1.219 .524 -1.21 6.21 Uji 3.9 mg/20 gBB 2.500 1.219 .524 -1.21 6.21

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91

Sat. 1.3 mg/20 gBB .000 1.219 1.000 -3.71 3.71 Sat. 2.6 mg/20 gBB -.250 1.219 1.000 -3.96 3.46 Descriptives Minggu Ke empat N Mean Std. Deviation Std. Error 95% Confidence Interval for Mean

Minimum Maximum Lower Bound Upper Bound K (-) 4 -1.00 1.155 .577 -2.84 .84 -2 0 K (+) Uji 4 .75 1.893 .946 -2.26 3.76 -2 2 K (+) Satelit 4 -2.50 1.291 .645 -4.55 -.45 -4 -1 Uji 1.3 mg/20 gBB 4 -2.00 2.160 1.080 -5.44 1.44 -4 1 Uji 2.6 mg/20 gBB 4 -2.25 .500 .250 -3.05 -1.45 -3 -2 Uji 3.9 mg/20 gBB 4 -2.25 1.893 .946 -5.26 .76 -5 -1 Sat. 1.3 mg/20 gBB 4 -2.00 1.414 .707 -4.25 .25 -3 0 Sat. 2.6 mg/20 gBB 4 -3.50 5.447 2.723 -12.17 5.17 -11 1

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92

Sat. 3.9 mg/20 gBB 4 -3.25 1.708 .854 -5.97 -.53 -5 -1

Total 36 -2.00 2.390 .398 -2.81 -1.19 -11 2

2.669 8 27 .027

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93

Descriptives Minggu Ke lima N Mean Std. Deviation Std. Error 95% Confidence Interval for Mean

Minimum Maximum Lower Bound Upper Bound K (+) Satelit 4 -1.00 1.155 .577 -2.84 .84 -2 0 Sat. 1.3 mg/20 gBB 4 -1.00 .816 .408 -2.30 .30 -2 0 Sat. 2.6 mg/20 gBB 4 -1.00 1.155 .577 -2.84 .84 -2 0 Sat. 3.9 mg/20 gBB 4 2.50 .577 .289 1.58 3.42 2 3 Total 16 -.13 1.784 .446 -1.08 .83 -2 3

4.000 3 12 .035

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94

Descriptives Minggu Ke enam N Mean Std. Deviation Std. Error 95% Confidence Interval for Mean

Minimum Maximum Lower Bound Upper Bound K (+) Satelit 4 1.75 .500 .250 .95 2.55 1 2 Sat. 1.3 mg/20 gBB 4 -1.75 .500 .250 -2.55 -.95 -2 -1 Sat. 2.6 mg/20 gBB 4 1.25 1.500 .750 -1.14 3.64 -1 2 Sat. 3.9 mg/20 gBB 4 2.00 3.162 1.581 -3.03 7.03 -1 6 Total 16 .81 2.228 .557 -.37 2.00 -2 6

6.823 3 12 .006

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95 LAMPIRAN C

Pengamatan Skrining Farmakologi Senyawa Asam

2-(3-(klorobenzoiloksi)benzoat

Tabel L.3.1. Data pengamatan skrining farmakologi kontrol negatif (PGA 3%) pada mencit jantan

Pengamatan

♂ T0’ T30’ T60’ T120Kontrol Negatif (PGA 3%) ’ T240 ’ T24 J T1 M T2 M T3 M T4 M Platform 39 19 21 17 29 37 29 31 23 27 Akt. Motorik A = 4 A = 2 A = 2 A = 4 A = 4 A = 4 A = 4 A = 4 A = 4 A = 4 Straub - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Piloereksi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Ptosis - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 R. pineal + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 R. Kornea + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Lakrimasi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Midriase - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Katalepsi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Sikap tubuh N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 Gelantung + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Retablisment + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Fleksi + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Haffner - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Kolik Ach - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Mortalitas - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Grooming - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Urinasi 1 2 - 1 - - - 1 - - Defekasi 1 1 - - 1 - 1 - 1 2 Keterangan :

Definisi operasional : pemberian subkronik adalah 28 hari ‘ : menit ; J : jam ; M : minggu

N : jumlah mencit (dinyatakan dengan angka) A : aktif ; D : diam ; N : normal

Platform : Jumlah jengukan per menit Straub (+) : Ekor hewan coba berdiri Piloereksi (+) : Bulu punggung hewan coba berdiri Ptosis (+) : Mata hewan coba menutup R. pineal (+) : Refleks pada tulang pina hewan coba R. kornea (+) : Refleks pada mata hewan coba Lakrimasi (+) : Hewan coba mengeluarkan air mata Midriase (+) : Pupil mata hewan coba melebar

Katalepsi (+) : Efek tidak nyaman namun tetap diikuti oleh hewan coba Sikap tubuh : Hewan coba segera membalikkan tubuhnya apabila diterlentangkan Gelantung (+) : Hewan coba mampu menggelantung dengan 2 tangannya

Retablisment (+) : Hewan coba mampu berjalan menggunakan ke empat kakinya pada alat uji gelantung Fleksi (+) : Refleks pada telapak kaki hewan coba

Haffner : Uji efek analgesik ; (+) : Ada efek analgesik Kolik Ach (+) : Hewan coba berjalan menggunakan perutnya Mortalitas (+) : Hewan coba mati

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96

Tabel L.3.2. Data pengamatan skrining farmakologi kontrol positif (AAS 1,3 mg/20 gBB) pada kelompok uji mencit jantan

Pengamatan

♂ T0’ T30’ T60’ Kontrol Positif (AAS 1,3 mg/20 gBB) T120 ’ T240 ’ T24 J T1 M T2 M T3 M T4 M Platform 57 19 5 15 25 18 19 20 25 17 Akt. Motorik A = 3 A = 2 A = 2 A = 3 A = 3 A = 3 A = 3 A = 3 A = 3 A = 2 Straub - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 + = 3 - = 4 - = 4 - = 4 Piloereksi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Ptosis - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 R. pineal + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 R. Kornea + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Lakrimasi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Midriase - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Katalepsi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Sikap tubuh N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 Gelantung + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Retablisment + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Fleksi + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Haffner - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Kolik Ach - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Mortalitas - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Grooming - = 4 - = 4 + = 3 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Urinasi 2 - - - - 1 - 1 - - Defekasi 1 - - - 1 - - 1 Keterangan :

Platform : Jumlah jengukan per menit

Straub (+) : Ekor hewan coba berdiri Piloereksi (+) : Bulu punggung hewan coba berdiri Ptosis (+) : Mata hewan coba menutup R. pineal (+) : Refleks pada tulang pina hewan coba R. kornea (+) : Refleks pada mata hewan coba Lakrimasi (+) : Hewan coba mengeluarkan air mata Midriase (+) : Pupil mata hewan coba melebar

Katalepsi (+) : Efek tidak nyaman namun tetap diikuti oleh hewan coba

Sikap tubuh : Hewan coba segera membalikkan tubuhnya apabila diterlentangkan Gelantung (+) : Hewan coba mampu menggelantung dengan 2 tangannya

Retablisment (+) : Hewan coba mampu berjalan menggunakan ke empat kakinya pada alat uji

gelantung

Fleksi (+) : Refleks pada telapak kaki hewan coba Haffner : Uji efek analgesik ; (+) : Ada efek analgesik Kolik Ach (+) : Hewan coba berjalan menggunakan perutnya Mortalitas (+) : Hewan coba mati

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97

Tabel L.3.3. Data pengamatan skrining farmakologi senyawa asam 2-(3-klorobenzoiloksi)benzoat 1,3 mg/20 gBB pada kelompok uji mencit jantan Pengamatan ♂ T0’ T30’ Asam 2-(3-klorobenzoiloksi)benzoat 1,3 mg/20 gBB T60’ T120 ’ T240 ’ T24 J T1 M T2 M T3 M T4 M Platform 25 14 8 5 5 7 13 9 10 8 Akt. Motorik A = 3 A = 3 A = 2 A = 1 A = 1 A = 2 A = 3 A = 2 A = 1 A = 1 Straub - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 + = 3 - = 4 Piloereksi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Ptosis - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 R. pineal + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 R. Kornea + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Lakrimasi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Midriase - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Katalepsi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Sikap tubuh N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 Gelantung + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Retablisment + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Fleksi + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Haffner - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Kolik Ach - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Mortalitas - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Grooming - = 4 - = 4 - = 4 + = 3 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Urinasi - - - 2 - - 1 Defekasi 1 - 1 1 - 1 - - 2 - Keterangan :

gelantung

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98

Tabel L.3.4. Data pengamatan skrining farmakologi senyawa asam 2-(3-klorobenzoiloksi)benzoat 2,6 mg/20 gBB pada kelompok uji mencit jantan Pengamatan ♂ T0’ T30’ Asam 2-(3-klorobenzoiloksi)benzoat 2,6 mg/20 gBB T60’ T120 ’ T240 ’ T24 J T1 M T2 M T3 M T4 M Platform 22 7 4 5 1 2 11 9 5 8 Akt. Motorik A = 3 A = 2 A = 2 A = 1 A = 1 A = 1 A = 2 A = 2 A = 1 A = 1 Straub - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 + = 3 - = 4 Piloereksi + = 3 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Ptosis - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 R. pineal + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 R. Kornea + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Lakrimasi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Midriase - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Katalepsi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Sikap tubuh N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 Gelantung + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Retablisment + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Fleksi + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Haffner - = 4 - = 4 - = 4 - = 4 + = 3 - = 4 - = 4 - = 4 - = 4 - = 4 Kolik Ach - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Mortalitas - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Grooming + = 3 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 + = 3 Urinasi - - - 1 - - - 2 - - Defekasi 2 - - - - 1 - - 1 - Keterangan :

gelantung

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99

Tabel L.3.5. Data pengamatan skrining farmakologi senyawa asam 2-(3-klorobenzoiloksi)benzoat 3,9 mg/20 gBB pada kelompok uji mencit jantan Pengamatan ♂ T0’ T30’ Asam 2-(3-klorobenzoiloksi)benzoat 3,9 mg/20 gBB T60’ T120 ’ T240 ’ T24 J T1 M T2 M T3 M T4 M Platform 30 18 9 5 4 8 16 13 9 11 Akt. Motorik A = 3 A = 3 A = 2 A = 1 A = 1 A = 1 A = 3 A = 2 A = 1 A = 2 Straub - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 + = 4 - = 4 Piloereksi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Ptosis - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 R. pineal + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 R. Kornea + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Lakrimasi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Midriase - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Katalepsi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Sikap tubuh N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 Gelantung + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Retablisment + = 4 + = 4 + = 4 - = 3 - = 2 + = 4 + = 4 + = 4 + = 4 + = 4 Fleksi + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Haffner - = 4 - = 4 + = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Kolik Ach - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Mortalitas - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Grooming - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 + = 3 + = 3 Urinasi - 1 - - - 1 - - 1 - Defekasi 2 1 - - - 1 - 2 - - Keterangan :

gelantung

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100

Tabel L.3.6. Data pengamatan skrining farmakologi kontrol positif (AAS 1,3 mg/20 gBB)pada kelompok satelit mencit jantan

Pengamatan

♂ T0’ T30’ T60’ Kontrol positif (AAS 1,3 mg/20 gBB) T120 ’ T240 ’ T24 J T1 M T2 M T3 M T4 M Platform 34 10 13 23 31 22 45 22 20 17 Akt. Motorik A = 3 A = 1 A = 1 A = 3 A = 3 A = 2 A = 3 A = 3 A = 3 A = 1 Straub - = 4 - = 4 - = 4 - = 4 + = 3 - = 4 + = 3 - = 4 - = 4 + = 3 Piloereksi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Ptosis - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 R. pineal + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 R. Kornea + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Lakrimasi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Midriase - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Katalepsi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Sikap tubuh N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 Gelantung + = 3 + = 2 + = 3 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 2 Retablisment + = 3 + = 2 + = 3 + = 4 + = 4 + = 4 + = 4 + = 4 + = 2 + = 2 Fleksi + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Haffner - = 4 - = 4 + = 3 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Kolik Ach - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Mortalitas - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Grooming - = 4 - = 4 + = 3 - = 4 - = 4 - = 4 - = 4 - = 4 + = 3 + = 3 Urinasi 3 1 - 2 - 1 `1 1 - - Defekasi - 2 - - - - 1 - - 2 Keterangan :

gelantung

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101

Tabel L.3.7. Data pengamatan skrining farmakologi senyawa asam 2-(3-klorobenzoiloksi)benzoat 1,3 mg/20 gBB pada kelompok satelit mencit jantan Pengamatan ♂ T0’ T30’ Asam 2-(3-klorobenzoiloksi)benzoat 1,3 mg/20 gBB T60’ T120 ’ T240 ’ T24 J T1 M T2 M T3 M T4 M Platform 39 22 25 16 12 15 10 8 11 13 Akt. Motorik A = 3 A = 3 A = 3 A = 2 A = 2 A = 1 A = 3 A = 3 A = 2 A = 1 Straub - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 + = 3 - = 4 Piloereksi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Ptosis - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 R. pineal + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 R. Kornea + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Lakrimasi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Midriase - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Katalepsi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Sikap tubuh N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 Gelantung + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Retablisment + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Fleksi + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Haffner - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Kolik Ach - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Mortalitas - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Grooming - = 4 - = 4 + = 3 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Urinasi 1 - - 1 - - - 2 - - Defekasi - 2 - 1 - - - 1 Keterangan :

gelantung

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102

Tabel L.3.8. Data pengamatan skrining farmakologi senyawa asam 2-(3-klorobenzoiloksi)benzoat 2,6 mg/20 gBB pada kelompok satelit mencit jantan Pengamatan ♂ T0’ T30’ Asam 2-(3-klorobenzoiloksi)benzoat 2,6 mg/20 gBB T60’ T120 ’ T240 ’ T24 J T1 M T2 M T3 M T4 M Platform 27 19 9 10 6 10 4 2 8 3 Akt. Motorik A = 3 A = 2 A = 2 A = 3 A = 1 A = 2 A = 2 A = 1 A = 2 A = 1 Straub - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 + = 3 + = 3 - = 3 Piloereksi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Ptosis - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 R. pineal + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 R. Kornea + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Lakrimasi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Midriase - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Katalepsi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Sikap tubuh N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 Gelantung + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Retablisment + = 4 + = 4 + = 4 + = 2 + = 2 + = 4 + = 4 + = 4 + = 4 + = 4 Fleksi + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Haffner - = 4 - = 4 - = 4 - = 4 + = 3 - = 4 - = 4 - = 4 - = 4 - = 4 Kolik Ach - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Mortalitas - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Grooming - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Urinasi 2 - 1 1 - - - 1 - 1 Defekasi - 1 - - - - 1 1 - 1 Keterangan :

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103

Tabel L.3.9. Data pengamatan skrining farmakologi senyawa asam 2-(3-klorobenzoiloksi)benzoat 3,9 mg/20 gBB pada kelompok satelit mencit jantan Pengamatan ♂ T0’ T30’ Asam 2-(3-klorobenzoiloksi)benzoat 3,9 mg/20 gBB T60’ T120 ’ T240 ’ T24 J T1 M T2 M T3 M T4 M Platform 42 26 13 11 10 15 12 9 11 7 Akt. Motorik A = 3 A = 3 A = 2 A = 1 A = 1 A = 2 A = 3 A = 2 A = 2 A = 2 Straub - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 + = 3 + = 3 Piloereksi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Ptosis - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 R. pineal + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 R. Kornea + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Lakrimasi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Midriase - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Katalepsi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Sikap tubuh N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 Gelantung + = 4 + = 4 + = 4 + = 3 + = 3 + = 4 + = 4 + = 4 + = 4 + = 4 Retablisment + = 4 + = 4 + = 4 + = 2 + = 1 + = 4 + = 4 + = 4 + = 4 + = 4 Fleksi + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Haffner - = 4 - = 4 + = 3 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Kolik Ach - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Mortalitas - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Grooming - = 4 + = 3 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 + = 3 Urinasi - 2 - - 1 - 2 - 1 - Defekasi - 1 - 1 - - - 1 - 1 Keterangan :

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104

Tabel L.3.10. Data pengamatan skrining farmakologi kontrol negatif (PGA 3%) pada mencit betina

Pengamatan

♀ T0’ T30’ T60’ T120Kontrol Negatif (PGA 3%) ’ T240 ’ T24 J T1 M T2 M T3 M T4 M Platform 55 32 25 30 34 21 34 29 31 24 Akt. Motorik A = 3 A = 2 A = 3 A = 3 A = 3 A = 3 A = 3 A = 2 A = 3 A = 3 Straub - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Piloereksi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Ptosis - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 R. pineal + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 R. Kornea + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Lakrimasi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Midriase - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Katalepsi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Sikap tubuh N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 Gelantung + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Retablisment + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Fleksi + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Haffner - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Kolik Ach - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Mortalitas - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Grooming - = 4 - = 4 - = 4 + = 3 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Urinasi 2 1 - - 1 - - 1 - - Defekasi 1 1 - 1 - 2 1 - 1 2 Keterangan :

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105

Tabel L.3.11. Data pengamatan skrining farmakologi kontrol positif (AAS 1,3 mg/20 gBB) pada kelompok uji mencit betina

Pengamatan

♀ T0’ T30’ T60’ Kontrol Positif (AAS 1,3 mg/20 gBB) T120 ’ T240 ’ T24 J T1 M T2 M T3 M T4 M Platform 60 21 7 13 20 19 17 21 22 17 Akt. Motorik A = 3 A = 2 A = 1 A = 3 A = 3 A = 3 A = 3 A = 3 A = 3 A = 2 Straub - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 + = 4 + = 4 Piloereksi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Ptosis - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 R. pineal + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 R. Kornea + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Lakrimasi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Midriase - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Katalepsi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Sikap tubuh N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 Gelantung + = 4 + = 2 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Retablisment + = 4 + = 2 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Fleksi + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Haffner - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Kolik Ach - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Mortalitas - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Grooming - = 4 - = 4 + = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Urinasi 1 1 - - - 1 - 1 - - Defekasi Keterangan :

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106

Tabel L.3.12. Data pengamatan skrining farmakologi senyawa asam 2-(3-klorobenzoiloksi)benzoat 1,3 mg/20 gBB pada kelompok uji mencit betina Pengamatan ♀ T0’ T30’ Asam 2-(3-klorobenzoiloksi)benzoat 1,3 mg/20 gBB T60’ T120 ’ T240 ’ T24 J T1 M T2 M T3 M T4 M Platform 35 17 7 8 3 9 11 9 3 7 Akt. Motorik A = 3 A = 3 A = 3 A = 2 A = 2 A = 3 A = 3 A = 2 A = 1 A = 2 Straub - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Piloereksi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Ptosis - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 R. pineal + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 R. Kornea + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Lakrimasi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Midriase - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Katalepsi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Sikap tubuh N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 Gelantung + = 4 + = 4 + = 4 + = 4 + = 3 + = 3 + = 4 + = 4 + = 4 + = 4 Retablisment + = 4 + = 4 + = 4 + = 4 + = 2 + = 3 + = 4 + = 4 + = 4 + = 4 Fleksi + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Haffner - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Kolik Ach - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Mortalitas - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Grooming - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 + = 4 Urinasi - 1 - 1 - 1 - 1 - - Defekasi - - 2 - 1 - - - - 1 Keterangan :

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107

Tabel L.3.13. Data pengamatan skrining farmakologi senyawa asam 2-(3-klorobenzoiloksi)benzoat 2,6 mg/20 gBB pada kelompok uji mencit betina Pengamatan ♀ T0’ T30’ Asam 2-(3-klorobenzoiloksi)benzoat 2,6 mg/20gBB T60’ T120 ’ T240 ’ T24 J T1 M T2 M T3 M T4 M Platform 22 18 13 14 8 9 19 10 12 7 Akt. Motorik A = 3 A = 3 A = 3 A = 3 A = 2 A = 3 A = 3 A = 2 A = 2 A = 2 Straub - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 + = 4 - = 4 Piloereksi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Ptosis - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 R. pineal + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 R. Kornea + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Lakrimasi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Midriase - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Katalepsi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Sikap tubuh N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 Gelantung + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Retablisment + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Fleksi + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Haffner - = 4 - = 4 + = 4 + = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Kolik Ach - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Mortalitas - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Grooming + = 4 - = 4 - = 4 - = 4 - = 4 + = 4 - = 4 - = 4 - = 4 + = 4 Urinasi 2 - 1 - - - 1 - - - Defekasi 1 - - 1 - 1 - - - - Keterangan :

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108

Tabel L.3.14. Data pengamatan skrining farmakologi senyawa asam 2-(3-klorobenzoiloksi)benzoat 3,9 mg/20 gBB pada kelompok uji mencit betina Pengamatan ♀ T0’ T30’ Asam 2-(3-klorobenzoiloksi)benzoat 3,9 mg/20gBB T60’ T120 ’ T240 ’ T24 J T1 M T2 M T3 M T4 M Platform 37 16 18 10 7 9 11 14 10 2 Akt. Motorik A = 3 A = 3 A = 3 A = 3 A = 2 A = 2 A = 3 A = 2 A = 2 A = 1 Straub - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 + = 3 + = 3 Piloereksi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Ptosis - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 R. pineal + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 R. Kornea + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Lakrimasi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Midriase - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Katalepsi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Sikap tubuh N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 Gelantung + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Retablisment + = 4 + = 4 + = 4 + = 4 - = 4 - = 4 + = 4 + = 4 + = 4 + = 4 Fleksi + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Haffner - = 4 - = 4 + = 4 + = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Kolik Ach - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Mortalitas - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Grooming + = 4 - = 4 - = 4 - = 4 - = 4 + = 4 - = 4 - = 4 - = 4 + = 4 Urinasi 2 - 1 - - - 1 - - - Defekasi 1 - - 1 - 1 - - - - Keterangan :

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109

Tabel L.3.15. Data pengamatan skrining farmakologi kontrol positif (AAS 1,3 mg/20 gBB)pada kelompok satelit mencit betina

Pengamatan

♀ T0’ T30’ T60’ Kontrol positif (AAS 1,3 mg/20 gBB) T120 ’ T240 ’ T24 J T1 M T2 M T3 M T4 M Platform 44 16 15 17 29 51 18 20 20 16 Akt. Motorik A = 3 A = 2 A = 2 A = 3 A = 3 A = 3 A = 3 A = 3 A = 3 A = 2 Straub - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 + = 4 - = 4 + = 4 + = 4 Piloereksi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Ptosis - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 R. pineal + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 R. Kornea + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Lakrimasi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Midriase - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Katalepsi - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Sikap tubuh N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 N = 4 Gelantung + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 3 + = 4 Retablisment + = 4 + = 2 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 2 + = 4 Fleksi + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 + = 4 Haffner - = 4 + = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 + = 4 - = 4 Kolik Ach - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Mortalitas - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Grooming - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 - = 4 Urinasi 2 - 3 - - 1 - 1 - 2 Defekasi - 1 - - 1 - 1 - 1 - Keterangan :

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