STUDI KOMPUTASI ISOPANDURATIN A, 4-HIDROKSIPANDURATIN A, KAEMPFERIDA DAN ETIL p-METOKSISINAMAT DARI TANAMAN SUKU ZINGIBERACEAE SEBAGAI INHIBITOR TIROSINASE DAN ALPHA MELANOCTYE STIMULATING HORMONE.

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iii ABSTRAK

Tirosinase dan alpha melanocyte stimulating hormone (α-MSH) berperan dalam proses pigmentasi kulit. Isopanduratin A dan 4-hidroksipanduratin A rimpang Kaempferia pandurata Roxb., kaempferida rimpang Alpinia officinarum Hance. dan etil p-metoksisinamat (EPMS) rimpang Kaempferia galanga L. terbukti memiliki aktivitas penghambatan tirosinase secara empiris dan in vitro. Studi docking molekul dilakukan untuk memvisualisasikan interaksi tingkat molekul keempat senyawa dengan tirosinase dan α-MSH kemudian aktivitasnya dibandingkan dengan arbutin, asam kojik, dan hidrokuinon. Hasil menunjukkan bahwa aktivitas penghambatan tirosinase dan α-MSH berturut-turut mulai dari yang terbaik adalah kaempferida, isopanduratin A, 4-hidroksipanduratin A, dan EPMS. Dapat disimpulkan senyawa tersebut lebih mudah berinteraksi dengan tirosinase dibandingkan α-MSH. Keempat senyawa kecuali EPMS berpotensi menghambat tirosinase lebih baik dibandingkan arbutin, asam kojik, dan hidrokuinon.

Kata kunci: Isopanduratin A, 4-Hidroksipanduratin A, Kaempferida, EPMS, Tirosinase, α-MSH, Docking molekul

FAKULTAS FARMASI

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iv ABSTRACT

Tyrosinase and alpha melanocyte stimulating hormone (α-MSH) involved in skin pigmentation process. Isopanduratin A and 4-hydroxypanduratin A of Kaempferia pandurata Roxb. rhizome, kaempferide of Alpinia officinarum Hance rhizome and ethyl p-metoxycinnamate (EPMS) of Kaempferia galanga L. rhizhome have shown inhibitory activity of tyrosinase both empirically and in vitro. Molecular docking studies has been performed to visualize molecular interaction of these compounds with tyrosinase and α-MSH then the activity was compared to arbutin, kojic acid, and hidroquinon. The results indicate that the inhibitory activity of tyrosinase and α-MSH in a row starting from the best are kaempferide, isopanduratin A, 4-hydroxypanduratin A, and EPMS. In conclusion these compounds interacted more easily with tyrosinase than α-MSH. These four compounds except EPMS potentially inhibit tyrosinase better than arbutin, kojic acid, and hydroquinone.

Key words: Isopanduratin A, 4-Hydroxypanduratin A, Kaempferide, EPMS, Tyrosinase , α-MSH, Molecular docking