ATOM INDONESIA
Author's Responses
Article : #425
Name of All Authors :
Article Title : Characterization Uptake and Cytotoxic of Radioiodine in MCF-7 and SKBR- 3 Breast Cancer Cell Lines
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Line # Referee’s Comments Author's Responses
1
The title should be slightly changed to:
Uptake and Cytotoxic Characterization of Radioiodine in MCF-7 and SKBR-3 Breast Cancer Cell Lines
Change is done
14 The abstract should be written in a single
paragraph Abstract in a single paragraph
19 …It presents Revised
45 .have been reported elsewhere […]
(references are required here) Revised 69 Were MCF-7 and Hacat cells certified? Yes, they were 73-74 Sentence needs revising: Hacat is normal
keratin cells, and in this investigation they were used as a control
done
91 …The cells were… done
152 Replace the word “non-significant” with
“insignificant” done
152-152 Why insignificant difference? Please discuss the reasons!
We find insignificant difference in radioiodine uptake between MCF-7 and Hacat cells. In our study, MCF-7 and Hacat cells do not express NIS, which is needed as iodine co-transporter (data do not publish).
We assume that insignificant difference in radioidine uptake between MCF-7 and Hacat cells is related to the absence of NIS
expression in both cells..
163-173
These earlier findings should be put in the introduction section instead of in the discussion section
Done
164-165 It is co-transports …. revised
166-167 Sodium gradient serves as…… and is
maintained by Change is done
245-256 This paragraph is not appropriate here. It
should be put in the introduction section. Is moved
262-273
This paragraph needs revising since there is lack of clarity due mostly to poor English grammar!
Also, why the percentages of colony formations 500 cells are different from those
revised
of 1000 cells?
274-282
Please check the grammar! Also, why the xytotoxic effect of radioiodine in MCF-7 cells are two-fold compared with that of SKBR-3 cells?
Further investigation in radiation biology is needed to cover up the different of
sensitivity between MCF-7 and SKBR3 cells toward radioiodine exposure
283-286 Why no effect for the control cells?
From this finding, we can conclude that Hacat as normal control cells do not become affected by radioiodine exposure
283-295 Please check the grammar 289-295
This is not your experimental result. Quote the reference(s)! What is the relationship between the first and second sentences in this paragraph?
References added Revised
310-315
This statement is probably incorrect. There are various research showing that radioiodine therapy has increased further cancer risks.
Check this websites:
http://www.breastcancer.org/research- news/20070604-2
http://www.cancernetwork.com/thyroid- cancer/increased-risk-second-
malignanciesradioactive-iodine-therapy- thyroid-cancer
http://erc.endocrinology-
journals.org/content/early/2012/07/31/ERC- 12-0176.full.pdf
Iodine -131 treatment gives to adult patients, so far we avoid to give I-131 to children or young patients as report as in
http://www.breastcancer.org/research- news/20070604-2
And also report that radioiodine increase 25% cancer risk do not state this research And also
(http://erc.endocrinology-
journals.org/content/early/2012/07/31/ERC- 12-0176.full.pdf)
Although case reports of several cancers(McCormack and Sheline, 1963, Kennedy and
Fish, 1959, Bundi et al., 1977, Spencer et al., 1983, Munoz et al., 1978) after radioactive iodine treatment have raised question about the carcinogenesis of 131I, the evidence from this
meta-analysis is lacking. In exposed patients, there was no increase in cancer risk overall.
Individual organ groups as well as a
combination of organ groups (CG) known to concentrate
radioiodine (salivary glands, digestive tract and urinary tract) did not also demonstrate an
increased risk of cancer. Individual studies of overall(Holm et al., 1991, Metso et al., 2007)
or CG(Hoffman et al., 1982) risks do report a slight increase in cancer but the overall burden
of risk by our population weighted estimate as well as conventional models concurred suggesting that overall burden of risk was null.
324-331 Put this paragraph in the experimental
section! Done
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