ATOM INDONESIA
2nd Author's Responses
Article : #745
Article Title : Radiolabeling Technique of Silver Nanoparticles (AgNPs) with Iodine-131 Radionuclide
Line # Referee’s Comments Author's Responses
1-2 The Title is Appropriate : partly correct
The title has been changed “Radiolabeling Technique of Silver Nanoparticles with Iodine-131 Radionuclide (AgNP-131I)” to
“Radiolabelling Technique of Silver Nanoparticles (AgNPs) with Iodine-131 Radionuclide”
12-13
There is a contradiction between statement Radiotherapy is an effective cancer therapy, specific to target but unaffected to normal tissue.
To overcome this issue, radioactive is attached to a carrier targeting specific organ.
The sentences have been changed as the suggested.
1st Paragr aph 15 – 36
Paragraph 1 is supposed to discuss only single claim or idea.
So it is suggested in the first paragraph you only discuss about what is radiopharmaceutical, diagnostic and therapeutic radiopharmaceuticals.
What is an ideal radiopharmaceuticals for targeted therapy
The 1st paragraph has been changed as suggested
2nd - 3rd paragr aph
In this paragraph you may discuss about nanomedicine agent and nano particle, silver nanoparticle (AgNPs).
Once AgNPs has been introduced….just used AgNPs in further discussion
The 2nd and 3rd paragraphs have been changed as suggested
4rd paragr aph
In this paragraph you may discuss why:
1. radiolabelled silver nanoparticle might be good for targeted radiopharmaceutical agent.
2. What you are going to do with silver nanoparticle
The 4th paragraph has been added the reasons
Four paragraphs for introduction should be sufficiently enough to introduce your presented work
111- 138
1. Do not write chemical name in capital except in the beginning of a sentence.
2. Pool all chemicals from the same manufacture and put manufacture’s name
Line 111-138 have been been changed as suggested
in the bracket, such a, b, c and d (Sigma- Aldrich)
3. Do not write instrument name in capital except in the beginning of a sentence, brand or model.
135 - 138
Radiolabeling of AgNPs with 131I was carried out in 3 steps: a) scaled synthesis of AgNPs and its purification, b) radiolabeling AgNPs using 131I and c) quality control of AgNPs -131I product.
Line 135-138 have been been changed as suggested
140 -
154 “Write here how AgNPs is synthesised”.
AgNPs synthesis was done by reactiong 5 mL AgNO3 0.001 N and reduced by 5 mL NaBH4 0.002 N then stabilized using 200 L PVP 0.3% (w/v) and 100L NaCl 1.5 N.
Measurement of Radionuclide and Radiochemical Purity of 131I
The parameters are radionuclide and radiochemical purity. Radionuclide purity of Na-
131I. 131I were measured using gamma ray spectrometer. Each sample was adsorbed on a rounded Whatman 1 filter paper, then added to the spacer and the energy was detected.
This paragraph was added as the suggested
156- 167
Radiolabeling AgNP with 131I
Immobilization of Chloramine-T on glass beads by performed by immersing 1 gram glass bead in 5 mL of 0.0005 M Chloramine-T for 30 min. The glass beads were separated into a clean vial. A solution of Na-131I (500 µL) was measured for its radioactivity using dose calibrator then added into a vial which contained immobilized of Chloramine-T glass beads (??) and allowed to react for 10 minutes. The solution of 1311 (???) which has been oxidised, was then transferred into a vial containing 1 mL of AgNP. The mixture allowed to react for 30 minutes at room temperature. Radioactivity of AgNPs-131I activity was then measured dose calibrator.
Note: Is there any purification step performed in this preparation???
In this step was done without further purification
171- 195
AgNPs-131I Quality Control
The quality control for AgNP-I is important parameter in order to estimate the radiolabeled compound (AgNP-131I) formed. Radiochemical purity determined by using thin layer chromatography (TLC) where Whatman paper No. 1 strip (1 cm x 17 cm) used as stationary phase and a mixture of methanol: water:
ammonium acetate (1: 1: 1, v:v:v) used as a mobile phase. A drop (5 L) of AgNPs-131I was spotted to Whatman paper No. 1 strip and was dried. The chromatographic strip was developed,
Line 171-195 have been changed as suggested
dried, then measured for its radioactivity distribution using a gamma counter. This procedure is also performed for Na-131I bulk as a standard in measuring the percentage of free Na-
131I (or Na-131I not attached to AgNPs).
Note: Radionuclide and radiochemical purity tests of 131I has to be performed before radiolabeling of AgNP with 131I. So you need a new paragraph just before a paragraph of Radiolabeling AgNP with 131I
198- 226
The mole ratio in this study, NaBH4 : AgNO3 = XX : XXX, was found to be the best for reduction processes which resulted AgNPs suitbale to be radiolabelled with 131I.
Line 227 -230 should be added to this paragraph.
Has been explored???. What do you mean by this? Do you use several reducing agents in this procedure? If only one type reducing agent you do not need to say it as explore…just use performed, done or other synonym
The sentence “The mole ratio in this study, NaBH4 : AgNO3 = XX : XXX”, has been changed into “The mole ratio in this study, NaBH4 : AgNO3 = 2 : 5”.
Line 227 -230 have been added to this paragraph.
The word explored “The preparation of silver nanoparticle have been explored” has been changed into “The preparation of silver nanoparticle have been performed”.
227- 252
Please indicate what is the wavelength shift would be categorized as significant??
According to the previous study, UV-Vis spectra recorded for biosynthesized of AgNPs were between 350 and 450 nm [What was its peak]
[17].
In this study result showed that the wavelength is still in range of SPR characteristic of AgNPs (350-450 nm).
The maximum peak shiftment only 1 nm so it is not significant.
The sentence “According to the previous study, UV-Vis spectra recorded for biosynthesized of AgNPs were between 350 and 450 nm“ has been change to “According to the previous study, UV-Vis spectra recorded for biosynthesized of AgNPs were 350 to 450 nm as the characteristic SPR of AgNPs “ [17].
Peak at the wavelength 350 to 450 nm is the SPR characteristic of AgNPs
248- 252
255- 264
This paragraph need to be moved to next paragraph.
Transmission electron microscopy data indicated that AgNPs size was below 10 nm [18] as shown in Fig. 2. The morphological form, size, stability of AgNPs is affected by experimental conditions, kinetic interactions of metal ions and the nanoparticles (NPs) adsorption with stabilizers [4].
Line 248-252 and 255-264 have been changed as suggested
287- The particle size analysis of AgNPs gave a This paragraph was changed as the
302 polydispersity index value of 0.455. The polydispersity index which has a value below 0.5 indidicates that the AgNPs were quite homogeneous. NPs could be called completely homogeneous when the index value of polydispersity is close to zero. The actual size of the synthesized-AgNPs is shown in Fig. 3. It can be seen from Fig. 3 the diameter by volume (Dv) of AgNPs, where the value of Dv10, Dv50 and Dv90 are 1.52, 3.79 nm and 7.65 nm respectively.
It means as much as 10, 50 and 90% of the total particle has a size below 1.52, 3.79 and 7.65 nm respectively. This corresponds to the TEM results which show that the AgNP size is below 10 nm.
suggested
311- 322
Radiolabeling of AgNPs using Radionuclide I- 131
A total 320 Ci/mL of the 131I solution which was incubated with immobilised Chloramine-T was used for labeling of AgNPs. The resulted AgNPs-
131I solution is a clear yellow solution as shown at Fig. 4. The color of AgNPs before and after radiolabeling with 131I were found to be similar.
This indicates that AgNPs- I is still stable without any aggregation. The AgNPs-131I radioactivity was measured using the Dose Calibrator, and the result was 96.5 µCi/mL. Fig.4. AgNPs-131I sample.
Note: for Figure 4. You should also show AgNPs prior radiolabeling
This paragraph was changed as the suggested. It have been added images of AgNPs prior radiolabelling
329- 343
Note: Radionuclide purity test result of 131I has to be discussed prior radiolabeling of AgNPs. Once Radionuclide purity test result of 131I has been found it is not neccessary to mesure the radionuclide purity of AgNPs-131I. So, this paragraph needs to be re-written!!!
Radionuclide Purity Test of Na-131I Bulk and AgNP-131 I
Based on gamma ray spectrometer analysis shows that Na-131I bulk and the AgNPs-131I have the same energy as listed in Table 1. Both Na-131I bulk and the AgNPs-131I have same energies but the count results are decrease. It means silver nanoparticle labelled Iodine-131. As shown at Fig. 5 a and b, the AgNPs-131I sample has more than 99.9% radionuclide purity (by gamma ray spectrometer) without other radionuclide impurities. This result complies with the radiopharmaceutical requirement for radionuclidic purity which must be = 99.9 % [19].
Line 329-343 were re-written and radionuclidic purity of AgNPs-131I were not measure.
383- 413
This paragraph needs to be given a title of
“Radiochemical purity test of AgNPs-131 I . This paragraph needs to be re-written!!! Please compare the result with other if there were any.
Radiochemical purity of of AgNPs-131I was measured by... The Iodine-131 bulk and radiolabeling results are presented in Fig. 6 a and b. The results of the Iodine-131 bulk and radiolabeled compound AgNPs-131I show different migration. The radionuclide 131I sample has retention factor (Rf) = 0.6, while the AgNPs-131I has Rf = 0. Based on peak chromatogram in fig 6.a, the Iodine-131 shows as I form. The radiochemical purity of 131I as the raw material was 98.42% and the product AgNPs-I was 96.49%. These parameters (radionuclidic and radiochemical purity) are important to comply with radiopharmaceutical standard [20]. Fig. 6.
Radiochromatogram of 131I (a) and AgNPs-131I (b).
Line 383-413 were changed as the suggested.
CONCLUSION
Synthesis of an AgNPs has been succesfully perfomed by XXXX. The synthesed AgNPs have spherical shape, the diameter was below 10 nm, and zeta potential value was -8 mV. The AgNPs then radiolabeled with 131I through chemisorption method to result in AgNPs-131I with radiochemical purity of xx%.
“Synthesis of an AgNPs has been succesfully perfomed by XXXX. The synthesed AgNPs have spherical shape, the diameter was below 10 nm, and zeta potential value was -8 mV. The AgNPs then radiolabeled with 131I through chemisorption method to result in AgNPs-131I with radiochemical purity of xx%” has ben changed to “The synthesis of an AgNPs has been succesfully perfomed by reduction method. The synthesized AgNPs have spherical shape, the diameter was below 10 nm, and zeta potential value was -8 mV. The AgNPs then radiolabeled with 131I through chemisorption method to result in AgNPs-
131I with radiochemical purity of 96.49%.”
Please return to Atom Indonesia Editorial Office via supplementary file in OJS aplication.
Date 8th March 2019
