ATOM INDONESIA
Author's Responses
Article : #741
Article Title : Pharmacokinetics Interaction of Non-Steroid Anti Inflammatory Drugs with
99mTc-MDP Radiopharmaceuticals for Bone Imaging
Line # Referee’s Comments Author's Responses
12-13
….drugs, meloxicam and natrium diclofenac with….….
….by injecting 100 mCi/ 100 mL
99mTc-MDP intravenously.
Blood samples were withdrawn from each mouse which were then weighted and counted using single channel analyzer.
The pharmacokinetic profile (%ID/g??) of
99mTc-MDP in blood of normal/ untreated (Group I), treated with meloxicam (Group II) and natrium diclofenac (Group III) mice 5 minutes post injection were 3.71, 8.96 and 9.15% respectively, then decrease to 0.12, 0.01, and 0.01%
respectively 24 hours post injection.
…T-test showed there was no….in distribution
99mTc-MDP of in normal/ untreated mice (Group I) and in treated mice either with meloxicam (Group II) or natrium diclofenac (Group III). However, there was significant difference in elimination of 99mTc-MDP in normal/ untreated mice (Group I) and in treated mice either with meloxicam (Group II) or natrium diclofenac (Group III).
Results of biodistribution study…please refer to correction 213-230. This part of abstract up to line 13 needs to be rewritten.
The injected dose is 100 µCi/100 µL
16-25 It’s suggested to be removed. It’s unnecessary
statement. Already revised
33 …compounds such as… Already revised
71 Remove The. Materials used… Already revised
74 …HCl dan acetone (E. Merck), NaCl 0.9%,
aquabidest (IPHA) Already revised
76 …3MM Whatman chromatography paper Already revised 78 …(Merck). In-house prepared MDP
radiopharmaceuticals kits. Already revised
84-90 These lines should be deleted. There are
duplications (see previous lines)!!! Already revised
93 All steps Already revised
93-96
Please do not use number as a subject of a sen- tence!!!
99mTc-Pertecnetate (3 mL, 3 mCi) was added into vial containing MDP kit.
Already revised
96 he solution was shook…..at room temperature re-
sulted in 99mTc-MDP radiopharmaceutical. Already revised
99
Radiochemical purity of 99mTc-MDP was determined using 2 paper chromatography system. The first one is using 3MM Whatman chromatography paper as stationary phase and 100% acetone for separating reduced- 99mTc (99mTcO2) and 99mTc-MDP from 99mTc-Pertecnetate.
The second one is using 3MM Whatman chromatography paper as stationary phase and 0.9% NaCl as a mobile phase for separating
99mTcO2 from 99mTc-MDP and 99mTc-Pertecnetate [Ref]
Already revised
108- 109
This research….. This sentence is suggested to be
removed. It’s unnecessary statement Already revised
115 …except in… Already revised
125 …test used… Already revised
128 and III. The treated mice were injected with
….injected through their tail veins Already revised
130 At specified time…. Already revised
132- 134
...99mTc-MDP, blood samples were withdrawn from the tail of each mouse. Blood samples were then weighted and counted using …single chan- nel analyzer Na(I)Tl detector.
Already revised
135
The results of measurement expressed as percent- age of injected dose per gram organ (blood in this case) (%ID/g) [x]
Already revised
137- 139
In order to determine the biological half-life of
99mTc-MDP the %ID/g of blood samples plotted again time of their withdrawal.
Please Note:
Instead of biological half-time most articles use term of biological half-life
Already revised
142- 144
It’s not clear what the meaning of this sentence is.
Is it t-test used to compare the biological half-life of 99mTc-MDP injected to mice in control group with treated group???
Need to be re-written.
Already revised
145
Biodistribution study performed on normal ani- mal group (I) and animal models group IV and V by injecting each mice with 0.1 mL of
99mTc-MDP (100 µCi) intravenously through the tail.
Already revised
149- 158
Please do not use then as a subject of a sentence.
Mice were then euthanized 3 hours post injection
Already revised
and vital organs such as muscle, bone,
blood, intestine, stomach, liver, spleen, kidneys, heart and lungs were taken. The collected samples weighed and then counted using single channel analyzer with Na(I)TL detector.
The %ID/g for each sample was then calculated.
163- 167
, vacumm….. What does is mean vacuum in this context???
________________________________________
__________________________________
Radiochemical purity is one of quality require- ments for a radiopharmaceutical in addition to clarity, sterility, non-pyrogenic and pH. Radio- chemical purity in this study was performed using 2 ascending paper chromatography paper sys- tems.
Already revised
167-
There is a miss-match between Figures and expla- nations.
Explanation Acetone (mobile phase) Figure Saline
Explanation Saline (mobile phase) Figure Ace- tone
--- --- Figure 1 should be changed to Figure 2 and vice versa!!!
Fig.1 shows a radiochromatogram of 99mTc-MDP where acetone used as a mobile phase, 99mTcO2
and 99mTc-MDP stayed on origin (Rf = 0) while
99mTc-pertecnetate moved with the solvent to give an Rf of 1.
In Fig. 2 on the hand shows a radiochromatogram of 99mTc-MDP where saline solution (0.9% NaCl) used as a mobile phase, 99mTcO2 stayed at origin while 99mTc-MDP and 99mTc-pertecnetate moved with the solvent to give an Rf of 1.
Already revised
174- 179
….respectively. This 99mTc-MDP conformed to the..
….radiochemical purity has to be greater than 95%.
Already revised 179-
181
The solution of MDP dissolved easily in saline, giving a clear and colorless solution, 180
vacuum (???) and pH between 5.8 – 6.0.??? Not sure the meaning of this sentence
Do you mean “The MDP kits (???) dissolved eas- ily in saline and gave a clear and colorless solution with pH of between 5.8 – 6.0.???. If yes,this sentence is not relevance as you were discussing 99mTc-MDP
Already revised
Or
The solution of 99mTc-MDP is a clear and color- less with pH between 5.8 and 6.0. ???
182- 184
The blood clearance profile tests were performed in order to determine changes in pharmacokinetic profiles and biological half-life of 99mTc-MDP in mice which had been previously treated with meloxicam and natrium diclofenac.
Already revised
184- The results in Fig. 3 ….. Already revised
188- 192
….profile (%ID/g??) of 99mTc-MDP in blood of normal/ untreated (Group I), treated with meloxi- cam (Group II) and natrium diclofenac (Group III) mice 5 minutes post injection were 3.71, 8.96 and 9.15% respectively, then decrease to 0.12, 0.01, and 0.01% respectively 24 hours post injec- tion.
--- ---
Note:
To make the statement much clearer, use normal/
untreated (Group I), treated with meloxicam (Group II) and natrium diclofenac (Group III) mice instead of Group I, II and III. Ths is in line with explanation on the graph (Fig. 3)
Already revised
193- 195
Based on the calculation of %ID/ g of blood, bio- logical half-life (distribution and elimination) can be determined as shown in Table.
--- --- Note:
1. The most common term used in biodistribution study is % ID/ g organ not radioactivity/ g organ.
2. Biological half-time is also not commonly used in biological study. Biological half-life is.
Already revised
196
---showed there was no….in distribution 99mTc- MDP of in normal/ untreated mice (Group I) and in treated mice either with meloxicam (Group II) or natrium diclofenac (Group III). However, there was significant difference in elimination of 99mTc- MDP in normal/ untreated mice (Group I) and in treated mice either with meloxicam (Group II) or natrium diclofenac (Group III).
Already revised
200- 205
To make the table to be easily read it is suggested to put some explanations/ legends as bellows:
Control/ Untreated mice (Group I) Treated with meloxicam (Group II)
Treated with natrium diclofenac (Group III).
Already revised
208- 211
…medicine, bone scintigraphy using 99mTc-MDP usually performed 2-5 hours post injection for al- lowing a clearance of administered..……
Already revised 213-
214
Biodistribution study (Fig. 4 and 5)…
…highest %ID/g organ was found in bone as the
Already revised
primary target of 99mTc-MDP radiopharmaceuti- cal.
--- ---
Be consistent!!
If you use Fig once use it all the time. Don’t use Fig and then Figure!!
216- 219
This sentences need to be re-written as it is not clear which data belong to Fig. 4 and which data belong to Fig 5.
________________________________________
__________________________________
It can be seen from Fig 4 that the % ID/ g organ (bone) of 99mTc-MDP were XX ± XX, XX ± XX, XX ± XX, XX ± XX, XX ± XX% in control/
untreated mice (Group I)
etc……..respectively…….xx hours post injec- tion.
It can be seen from Fig 5 that the % ID/ g organ (bone) of 99mTc-MDP were XX ± XX etc.
See the above-mentioned example!!!
Already revised
219- 230
This sentences need to be re-written!!!
…….respectively. It can be seen from these re- sults that there were no significant different in bone uptake of 99mTc-MDP in mice with previ- ously treated with meloxicam………etc
Already revised
231 99mTc-MDP bone uptake…. Already revised
255 …99mTc-MDP bone uptake too. Already revised 319-
323 Move Fig 2 to Fig 1 and vise versa Already revised
325- 327
Radioactivity (%ID/g) …should be only %ID/g”:
Suggested Legend change:
Normal Normal mice
Natrium Dicofenac Mice treated with Normal Meloxicam Mice treated with Meloxicam
Already revised
329-
335 Same suggestion as line 325-335 Already revised
Please return to Atom Indonesia Editorial Office via supplementary file in OJS aplication.
6/6/2018
