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ATOM INDONESIA

Author's Responses

Article : #425

Name of All Authors :

Article Title : Effect of Gamma Irradiation on Nitrate-Reducing Bacteria in Decreasing of Methane Production in vitro by Buffalo Rumen Liquid

E-mail :

Line # Referee’s Comments Author's Responses

1

Title change: Effect of Gamma Irradiation on Nitrate-Reducing Bacteria in Decreasing of Methane Production in vitro by Buffalo Rumen Liquid

Has been revised.

64

Please correct the sentence, grammatically is mistaking (rearrange the sentence). Please pay attention in grammatical

Has been revised.

78 Multipicating???

Probably: multiplicating Has been revised.

79 the growing cells was inoculated (grammar ???)

the growing cells were inoculated (plural) Has been revised.

88-89

inactivated by gamma irradiator (1500 Gy; rate doses 20 kGy/h) and autoclaved (1210C; 15 minutes).

change: inactivated by gamma irradiation with a dose of 1500 Gy; doses rate of 20 kGy/h or by autoclaved (1210C; 15 minutes)

Has been revised.

99-104

Please explain how do you determine each of parameter (The parameter measured were pH, ammonia, total volatile fatty acids (VFA), acetic, propionic and butyric acid as partial VFA, microbial synthesis protein by radioisotope of 32P [14], digestibility of organic matters

(DOM), gas production and methane production

Has been revised.

109 Data was analyzed

Change Data were analyzed Has been revised.

134 Opposite to another study report

Change: Opposite to another study reported Has been revised.

137- 148

Please explain why do you have different pH results from others (you got pH decreases by incubation time while Sakthivel P.C Ref. 9 got increase pH), it is good for rumen microbial activities i.e. 6.5–7. Elaborate in more detail

Has been revised.

308

Conclusion is not reflected the present study (this paper). It should be corrected, the

summary of this paper should be the conclusion

Has been revised.

315

ACKNOWLEDGMENT

Statement in ACKNOWLEDGMENT is not clear in what thing you will acknowledge

Has been revised.

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BATAN and UIN (eg. Supporting of budget, facility etc)

Please return to Atom Indonesia Editorial Office via e-mail:

[email protected]

and Faximile: 021-7560895.

This original sheet should be returned to:

Administrator Atom Indonesia, PPIKSN-BATAN, Gedung 71, Lantai 1, Kawasan Puspiptek Serpong, Tangerang, Indonesia 15310

Author Signature(s) Date

Name(s) (print)

Referensi

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