ATOM INDONESIA
Author's Responses
Article : #529
Article Title : Effect of Gamma Irradiated Nitrate Reducing Bacteria on the Methane Production and Fermentation in Vitro by Buffaloes Rumen Liquid
Line # Referee’s Comments Author's Responses
1-3
Title: The Effect of Gamma Irradiation on Nitrate-Reducing Bacteria (NRB) in Decreasing of Methane Production In Vitro by Buffalo Rumen Liquid
It has been revised to be:
The Effect of Gamma Irradiated Nitrate- Reducing Bacteria in Decreasing the in vitro Production of Methane by Buffalo Rumen Liquid
10
Abstract: Ruminant livestock have a contribution in in increasing the effect ---
--- applying nitrate-reducing bacteria (NRB).
Utilizing active NRB has a side effect that they it causes acidosis
liquid. The examination was applied in vitro--- Please check and recheck sentence by sentence!!
There were differences in pH values, digestibility of organic matters, ammonia production?, total
and partial volatile fatty acids,………..
The irradiated NRB produced methane slightly higher, i.e. 2.97 and 10.42 ml/200 mg after 24 and 48 h incubation respectively, compared to the
active, autoclaved and without the addition of NRB, i.e. 2.01 and 8.02; 7.82 and 11.72; 8.0 and
12.36 ml/200 mg respectively.
The value is significantly different each other, so you do not need to use 2 decimals. 3.0, 10.4 --- ---2.0, 8.0, 11.7. 8.0, 12.4 is more simple.
Thank you, we have corrected them as recommended.
19-20
---- more harmful than the other gasses, i.e. the carbon dioxide (CO2) and the nitrous oxide (N2O) [1].
Thank you, we have corrected them as recommended.
35 gasses to will create a better environment [5]. Thank you, we have corrected them as recommended.
43 ---- utilizing of hydrogen [6,7,8]. Removing of ref. no. 7-8 will reduce the old references
Thank you for the advice, but we apologize for not complying because by including the references we show the importance of this phenomenon that has been studied in previous studies.
45 in vitro → in vitro Thank you, we have corrected them as recommended.
66-70 Please use passive sentence Therefore, our current study was to examine the using of the
Thank you, we have corrected them as recommended.
NRB isolated from the rumen, inactive and inactive conditions --- Please use passive sentence! Therefore, the current study was to examine the using use of the NRB isolated from the rumen, inactive and inactive conditions --- --(both is inactive?)
74
Isolate KS1 was --- Is it common name, so everyone (every reader) could understand? It is better you explain it, since it first time appeared
Thank you, we have corrected them as recommended.
76 ---- agar (0.5g KNO3, 0.1m --- → KNO3
Thank you, we have corrected them as recommended.
78 ….Probably: multiplicating Thank you, we have corrected them as recommended.
79-80 For multiplication, of the cell culture, the growing cells was were
Thank you, we have corrected them as recommended.
90-91
gamma irradiation with dose of 1500 Gy; rate doses 20 kGy/h) or byautoclaved → gamma irradiator with irradiation at a dose of 1.5 kGy with a dose rate of 20 kGy/h) by autoclave or…….by autoclaved (choose one)
Thank you, we have corrected them as recommended.
92 Determination of in vitro Thank you, we have corrected them as recommended.
94 substrate were prepared with four treatments: A Thank you, we added “i.e.”
99-104 Please explain how do you determine each of parameter
Thank you for the suggestion. We added the explanation.
100 ----ammonia,--- →ammonia production Thank you, we have corrected them as recommended.
103 ---concentration) by Gas Chromatography method [15], ---- → gas chromatograph method
Thank you, we have corrected them as recommended.
104 microbial synthesis protein by--- → microbial protein synthesis
Thank you, we have corrected them as recommended.
109 →The change data was--- Thank you, we have corrected them as recommended.
122 ----ammonia,--- →ammonia production Thank you, we have corrected them as recommended.
123 … partial VFA and CH4 production Thank you, we have corrected them as recommended.
130- 132
A: active NRB; B: γ-irradiated NRB; C:
autoclaved NRB; D: without NRB; DOM:
Digestibility of Organic Matters. Different superscript letter (abcd 132 ) through a column shows a significant difference---
Thank you, we have corrected them as recommended.
134
Opposite to another study reported that … → Different to another study, it was reported that --- ----
Thank you, we have corrected them as recommended.
137-
148 …..Elaborate in more detail Thank you, we have corrected them as recommended.
176- 178
It is an intermediate compound before the formation of methane produced by types of acetonotrophic bacteria. → There was an intermediate compound produced by types of acetonotrophic bacteria before the formation of methane.
Thank you, we have corrected them as recommended.
180 --- indicated the inhibiting of acetate production - ---- → --- indicated the inhibition of acetate
Thank you, we have corrected them as recommended.
production --- or --- indicated an inhibiting the acetate production ---
221 hydrogen transfer. H2 → H2
Thank you, we have corrected them as recommended.
238- 241
If one decimal does not cause a significant change, please use one decimal.
Thank you, we have corrected them as recommended.
242-
255 Please Fig. 1B change also in similar manner Thank you, we have corrected them as recommended.
260 10.7 and 18.6% Thank you, we have corrected them as
recommended.
261-
263 The value was…….This proved-- Thank you, we have corrected them as recommended.
276-
277 In addition, the inoculated-- Thank you, we have corrected them as recommended.
283 It is proposed Thank you, we have corrected them as
recommended.
308- 312
Conclusion is not reflected the present study (this paper). It should be corrected, the summary of this paper should be the conclusion.
310
If you use the term” The addition” of the γ- irradiated NRB to --- has (youn should put something in --- Otherwise…….you can remove “The addition”
Thank you, we have corrected them as recommended.
315 Statement in ACKNOWLEDGEMENT is not clear
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