52
Bioinformatic and In-vitro Characterization of scaF as a Novel Gene for Identification of Staphylococcus aureus Isolates
Mohammad Reza Pourmand1, Elaheh Salami2, Jalil Fallah Mehrabadi3, Azar Hadadi4, Rahil Mashhadi5
1 Associate Professor, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2 MSc Student in Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences,
Tehran, Iran
3 Assistant Professor, Department of Bioscience and Biotechnology, Malekashtar University of Technology, Tehran, Iran 4 Associate Professor, Department of Infectious Diseases, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran
5 M.Sc. in Cellular and Molecular Biology, Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran
(Received May 31, 2014 ; Accepted August 9, 2014)
Abstract
Background and purpose: Rapid detection of Staphylococcus aureus in clinical specimens is essential to minimize the transfer and spread of this pathogen. An appropriate method for rapid and suitable detection of S. aureus is detection of one of its genes. The aim of this study was to identify the S.
aureus using scaF gene.
Material and Methods: The study was conducted on 45 isolates of S. aureus separated from clinical specimens of patients who referred to hospitals affiliated with Tehran University of Medical Sciences in 2013. At first, scaF gene was analyzed by bioinformatic tools. S.aureus isolates were confirmed using biochemical and diagnostic tests. Following DNA extraction, scaF gene was amplified by polymerase chain reaction using gene-specific primers. The results of PCR were examined by gel electrophoresis.
Results: The scaF gene was present in all studied isolates. Bioinformatics evaluation revealed that this gene encodes a protein with a molecular weight of 33kDa and contains 297amino acids with a PI value of 6.3.
Conclusion: The scaF gene belongs to Staphylococcal conserved antigen family which is conserved in S. aureus strains recorded in JCVI database. Furthermore, due to lack of the same gene in Staphylococcus epidermidis and other species, it could be used for detection of S. aureus infections.
Keywords: Staphylococcus aureus, polymerase chain reaction, detection, scaF
J Mazandaran Univ Med Sci 2014; 24(116): 52-57 (Persian).
هرود هرﺎﻤﺷ مرﺎﻬﭼ و ﺖﺴﻴﺑ 116
رﻮﻳﺮﻬﺷ لﺎﺳ 1393 ) 57 - 52 (
ﺪﻳﺪﺟ نژ ﻲﻫﺎﮕﺸﻳﺎﻣزآ و ﻲﻜﻴﺗﺎﻣرﻮﻔﻧاﻮﻴﺑ ﻲﺳرﺮﺑ ﺖﻬﺟ scaF
سﻮﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘﺳا يﺎﻫ ﻪﻟوﺰﻳا ﻲﻳﺎﺳﺎﻨﺷ
ﺪﻨﻣرﻮﭘ ﺎﺿرﺪﻤﺤﻣ 1
ﻲﻤﻴﻠﺳ ﻪﻬﻟا 2
يدﺎﺑآﺮﻬﻣ حﻼﻓ ﻞﻴﻠﺟ 3
يداﺪﺣ رذآ 4
يﺪﻬﺸﻣ ﻞﻴﺣار 5
هﺪﻴﻜﭼ
ﻪﻘﺑﺎﺳ
فﺪﻫ و : ﻪﻧﻮﮔ ﻲﻣﻮﻧژ عﻮﻨﺗ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ و ﺎﻫ
ﻪﻳﻮﺳ ﻠﻴﻓﺎﺘﺳا يﺎﻫ سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘـﺳا يﺮﺘﻛﺎﺑ ﻊﻳﺮﺳ ﺺﻴﺨﺸﺗ ،سﻮﻛﻮﻛﻮ
ﻪﻧﻮﻤﻧ رد سﻮﺋروا
يروﺮﺿ يﺮﻣا نژﻮﺗﺎﭘ ﻦﻳا شﺮﺘﺴﮔ و لﺎﻘﺘﻧا ﺶﻫﺎﻛ ياﺮﺑ ﻲﻨﻴﻟﺎﺑ يﺎﻫ ﺖﺳا
.
هار زا ﻲﻜﻳ يﺎﻫ
،ﻊﻳﺮﺳ ﺺﻴﺨﺸﺗ
نژ زا ﻲﻜﻳ بﺎﺨﺘﻧا
ﺖﺳا ﻲﻳﺎﺳﺎﻨﺷ ﺖﻬﺟ نآ يﺎﻫ .
ﻪـﻟوﺰﻳا ﻲﻳﺎـﺳﺎﻨﺷ ،ﻪﻌﻟﺎﻄﻣ ﻦﻳا زا فﺪﻫ ﺎـﺑ سﻮـﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘـﺳا يﺎـﻫ
هدﺎﻔﺘﺳا نژ زا ﻲﻣscaF
ﺪﺷﺎﺑ .
ﺎﻫ شور و داﻮﻣ :
ﻪﻌﻟﺎﻄﻣ ﻦﻳا لﺎﺳ رد
1392 يور 45
ﻪـﻧﻮﻤﻧ زا هﺪـﺷ اﺪـﺟ سﻮﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘﺳا ﻪﻟوﺰﻳا ﻲﻨﻴﻟﺎـﺑ يﺎـﻫ
نﺎﺘﺳرﺎﻤﻴﺑ
ﺖـﻓﺮﮔ مﺎﺠﻧا ناﺮﻬﺗ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد يﺎﻫ .
نژ اﺪـﺘﺑا scaF
مﺮـﻧ ﻚـﻤﻛ ﺎـﺑ راﺮـﻗ ﻲـﺳرﺮﺑ درﻮـﻣ بو يﺎـﻫراﺰﻓا
ﺖﻓﺮﮔ .
شور ﻚﻤﻛ ﺎﺑ ﻪﻣادا رد يﺎﻫ
نﻮﻣزآ و ﻲﻳﺎﻴﻤﻴﺷﻮﻴﺑ
ﻪﻟوﺰﻳا ،ﻲﺼﻴﺨﺸﺗ يﺎﻫ ﺪـﻴﺋﺎﺗ درﻮـﻣ سﻮـﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘﺳا يﺎﻫ
ﺪﻨﺘﻓﺮﮔ راﺮﻗ .
جاﺮﺨﺘﺳا ﺐﻗﺎﻌﺘﻣ
DNA
نژ ،ﻲﻣﻮﻧژ scaF
هﺮﻴﺠﻧز ﺶﻨﻛاو ﺎﺑ
ﻪﻟوﺰﻳا ﻦﻳا رد ﻲﺻﺎﺼﺘﺧا يﺎﻫﺮﻤﻳاﺮﭘ و زاﺮﻤﻴﻠﭘ يا ﺎﻫ
ﺖﻓﺎﻳ ﺮﻴﺜﻜﺗ .
زا ﻞﺻﺎﺣ ﺞﻳﺎﺘﻧ )
(PCR
polymerase chainreaction
ﻜﻟا لژ شور ﻂﺳﻮﺗ ﺪﻨﺘﻓﺮﮔ راﺮﻗ ﻲﺳرﺮﺑ درﻮﻣ زرﻮﻓوﺮﺘ
.
ﺎﻫ ﻪﺘﻓﺎﻳ :
ﻪﻟوﺰﻳا ﻪﻤﻫ رد
دﻮﺟو ﻪﻌﻟﺎﻄﻣ درﻮﻣ يﺎﻫ نژ
scaF
ﺪﻳدﺮﮔ ﺖﺒﺜﻣ ، .
،نژ ﻦـﻳا ﻪـﻛ داد نﺎﺸﻧ ﻚﻴﺗﺎﻣرﻮﻔﻧاﻮﻴﺑ ﻲﺳرﺮﺑ
ﻲﻟﻮﻜﻟﻮﻣ نزو ﻪﺑ ﻲﻨﻴﺌﺗوﺮﭘ 33
ياراد ﻪﻛ هدﺮﻛ ﺪﻛ ار نﻮﺘﻟادﻮﻠﻴﻛ 297
نآ ﻚﻳﺮﺘﻜﻟاوﺰﻳا ﻪﻄﻘﻧ و ﺖﺳا ﻪﻨﻴﻣآﺪﻴﺳا 3
/ 6 ﻲـﻣ ﺪـﺷﺎﺑ .
ﻳا نﻮﻨﻛﺎﺗ ﺖﺳا ﻪﺘﻓﺮﮕﻧ راﺮﻗ ﻲﻫﺎﮕﺸﻳﺎﻣزآ ﻲﺳرﺮﺑ درﻮﻣ ﻦﻴﺌﺗوﺮﭘ ﻦ .
جﺎﺘﻨﺘــﺳا نژ:
ﻲــﻧژ هداﻮﻧﺎــﺧ ﻪــﺑ ﻖــﻠﻌﺘﻣ scaF Staphylococcal conserved antigens
ﻪﻳﻮــﺳ رد ﻪــﻛ هدﻮــﺑ يﺎــﻫ
ﻲﺗﺎﻋﻼﻃا هﺎﮕﻳﺎﭘ رد هﺪﺷ ﺖﺒﺛ سﻮﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘﺳا JCVI
ﻲﻣ هﺪﺷ ﺖﻇﺎﻔﺣ ، ﺪﺷﺎﺑ
.
،ﻦﻳا ﺮﺑ هوﻼﻋ نژ دﻮﺟو مﺪﻋ ﺖﻠﻋ ﻪﺑ
رد ﻪﺑﺎﺸﻣ
ﻪﻧﻮﮔ ﺮﻳﺎﺳ و ﺲﻳﺪﻴﻣرﺪﻴﭘا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘﺳا ﻲﻣ ﺎﻫ
ﺖﻧﻮﻔﻋ رد سﻮﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘﺳا ﺺﻴﺨﺸﺗ ياﺮﺑ نآ زا ناﻮﺗ ﺎـﻫ
دﺮﻛ هدﺎﻔﺘﺳا .
يﺪﻴﻠﻛ يﺎﻫ هژاو
،سﻮﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘﺳا :
،زاﺮﻤﻴﻠﭘ يا هﺮﻴﺠﻧز ﺶﻨﻛاو )
(PCR
، scaF
ﻪﻣﺪﻘﻣ
ﻢﻬﻣ زا ﻲﻜﻳ سﻮﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘﺳا ﻞـﻣاﻮﻋ ﻦﻳﺮﺗ
ﺖﻧﻮﻔﻋ
ﻧﺎﺘـﺳرﺎﻤﻴﺑ يﺎـﻫ ﻪـﻛ هدﻮـﺑ ﻪـﻌﻣﺎﺟ زا ﻲﺑﺎﺴـﺘﻛا و ﻲ
ﻲﻣ
ﺖـﻧﻮﻔﻋ ﻞﻣﺎﻋ ﺪﻧاﻮﺗ ،ﻲﻤﻳﺮﺘﻛﺎـﺑ نﻮـﭽﻤﻫ ﻲـﻤﻬﻣ يﺎـﻫ
و ﻚﻴﺴﻛﻮﺗ كﻮﺷ مرﺪﻨﺳ ،ﺖﻴﻠﻴﻣﻮﺌﺘﺳا ،ﺖﻳدرﺎﻛوﺪﻧا
لﻮﺌﺴﻣ ﻒﻟﻮﻣ :
ﺪﻨﻣرﻮﭘ ﺎﺿرﺪﻤﺤﻣ ناﺮﻬﺗ-
- ناﺮﻬﺗ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،ﺖﺷاﺪﻬﺑ هﺪﻜﺸﻧاد ،يژﻮﻟﻮﻴﺑﻮﺗﺎﭘ هوﺮﮔ ﻲﺳﺎﻨﺷ بوﺮﻜﻴﻣ
E-mail:[email protected]
1 . ناﺮﻬﺗ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ، ﺖﺷاﺪﻬﺑ هﺪﻜﺸﻧاد ، يژﻮﻟﻮﻴﺑﻮﺗﺎﭘ هوﺮﮔ ﻲﺳﺎﻨﺷ بوﺮﻜﻴﻣ رﺎﻴﺸﻧاد
2 . ناﺮﻬﺗ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،ﺖﺷاﺪﻬﺑ هﺪﻜﺸﻧاد ، يژﻮﻟﻮﻴﺑﻮﺗﺎﭘ هوﺮﮔ ،ﻲﺳﺎﻨﺷ بوﺮﻜﻴﻣ ﺪﺷرا ﻲﺳﺎﻨﺷرﺎﻛ يﻮﺠﺸﻧاد
3 . هﺪﻜﺸﻫوﮋﭘ ﻲﺳﺎﻨﺷ بوﺮﻜﻴﻣ رﺎﻳدﺎﺘﺳا مﻮﻠﻋ
يروﺎﻨﻓو ﺎﻣ ﻲﺘﻌﻨﺻ هﺎﮕﺸﻧاد ،ﻲﺘﺴﻳز ﺮﺘﺷا ﻚﻟ
4 . ناﺮﻬﺗ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،ﺎﻨﻴﺳ نﺎﺘﺳرﺎﻤﻴﺑ ،ﻲﻧﻮﻔﻋ يﺎﻫ يرﺎﻤﻴﺑ رﺎﻴﺸﻧاد
5 . ناﺮﻬﺗ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ، يژﻮﻟورا تﺎﻘﻴﻘﺤﺗ ﺰﻛﺮﻣ ، ﻲﻟﻮﻜﻟﻮﻣ و ﻲﻟﻮﻠﺳ ﺪﺷرا سﺎﻨﺷرﺎﻛ
ﺖﻓﺎﻳرد ﺦﻳرﺎﺗ :
10 / 3 / 1393 تﺎﺣﻼﺻا ﺖﻬﺟ عﺎﺟرا ﺦﻳرﺎﺗ
: 10 / 4 / 1393 ﺐﻳﻮﺼﺗ ﺦﻳرﺎﺗ :
18 / 5 / 1393
نژ ﻲﻫﺎﮕﺸﻳﺎﻣزآ و ﻲﻜﻴﺗﺎﻣرﻮﻔﻧاﻮﻴﺑ ﻲﺳرﺮﺑ
نارﺪﻧزﺎﻣ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻪﻠﺠﻣ
هرود
مرﺎﻬﭼ و ﺖﺴﻴﺑ
، هرﺎﻤﺷ 116 رﻮﻳﺮﻬﺷ ،
1393 54
ﺖﻧﻮﻔﻋ
ﺪﺷﺎﺑ ﻲﺘﺳﻮﭘ يﺎﻫ
) 2،1
ﻢﻫ .(
ترﺪـﻗ ﻞـﻴﻟد ﻪـﺑ ﻦﻴـﻨﭼ
يرﺎﻤﻴﺑ سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘﺳا نوﺰﻓازور ﺖﻣوﺎﻘﻣ و هﻮﻘﻟﺎﺑ ﻲﻳاز ﻪﺒﻋﻮـﺿﻮﻣ ﻦـﻳا ،ﻲﺑوﺮﻜﻴﻣﺪﺿ يﺎﻫوراد ﺮﺑاﺮﺑ رد سﻮﺋروا
ﻢﻬﻣ زا ﻲﻜﻳ ﻞﻳﺪـﺒﺗ نﺎـﻬﺟ رد ﻲﺘـﺷاﺪﻬﺑ تﻼﻜﺸـﻣ ﻦﻳﺮـﺗ
ﺖـﺳا هﺪﺷ
) 3
.(
ﺺﻴﺨﺸـﺗ رد ﺖـﻧﻮﻔﻋ
ﻪـﻧﻮﻤﻧ ،ﻲﻨﻴﻟﺎـﺑ يﺎـﻫ
زا ،هﺎــﺗﻮﻛ نﺎــﻣز رد ﻲﻧﻮــﻔﻋ ﻞــﻣﺎﻋ ﻲﻟﻮــﻜﻟﻮﻣ ﻲﻳﺎــﺳﺎﻨﺷ
ﺖــﺳا رادرﻮــﺧﺮﺑ يدﺎــﻳز رﺎﻴﺴــﺑ ﺖــﻴﻤﻫا
) 4
.(
شور يﺎــﻫ
و ﻲﭙﻴﺗﻮــﻨﻓ يﺎــﻫ ﻲــﮔﮋﻳو ﺮــﺑ ﻲــﻨﺘﺒﻣ لواﺪــﺘﻣ ﻲﺼــﻴﺨﺸﺗ ﺮـﺑ نﺎـﻣز نﻮﭽﻤﻫ ﻲﺒﻳﺎﻌﻣ ياراد ﻲﻳﺎﻴﻤﻴﺷﻮﻴﺑ يﺎﻫ ﺶﻨﻛاو
ﺪﻨﺷﺎﺑ ﻲﻣ ندﻮﺑ
) 7 - 5
.(
شور هزوﺮﻣا ﺺﻴﺨﺸـﺗ ﻊﻳﺮـﺳ يﺎـﻫ
ﻦـﻳا يﺮﻴﮔرﺎـﻛ ﻪـﺑ و ﻪـﺘﻓﺎﻳ شﺮﺘﺴـﮔ ﺎﻫ نژﻮﺗﺎﭘ ﻲﻟﻮﻜﻟﻮﻣ زا ار ﻲﻧﻮـﻔﻋ ﻞـﻣﺎﻋ ﺺﻴﺨﺸـﺗ نﺎﻣز ،ﺎﻫ شور 2
ﺎـﺗ 3 زور
)
شور زﺎﻴﻧ درﻮﻣ ﻲﭙﻴﺗﻮﻨﻓ يﺎﻫ
(
ﺎـﻛ ﺖﻋﺎـﺳ ﻦﻳﺪﻨﭼ ﻪﺑ ﺶﻫ
ﻪـــﻣﺎﻧﺮﺑ و ﺪـــﻫد ﻲـــﻣ دﻮـــﺒﻬﺑ ار ﺖﻣﻼـــﺳ لﺮـــﺘﻨﻛ يﺎـــﻫ
ﻲﻣ ﺪﻨﺸﺨﺑ
) 8
نژ.(
)scaF sacol0270
( هداﻮﻧﺎـﺧ ﻪـﺑ ﻖﻠﻌﺘﻣ
ﺎﻳ Sca Staphylococcal conserved antigens
ﻲﻣ ﺪﺷﺎﺑ
نﻮﻨﻛﺎﺗ ﻪﻛ نآ درﻮﻣ رد ﻲﻟﻮﻜﻟﻮﻣ و ﻲﻫﺎﮕﺸﻳﺎﻣزآ ﻲﺳرﺮﺑ
ﺖــــﺳا ﻪــــﺘﻓﺮﮕﻧ ترﻮــــﺻ نﻮﻨﻛﺎــــﺗ .
هداﻮﻧﺎــــﺧ رد Sca
ﺎﺘـــــﺳا و سﻮـــــﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘـــــﺳا سﻮﻛﻮﻛﻮﻠﻴﻓ
ﻪـﻋﻮﻤﺠﻣ ﺐﻴﺗﺮﺗ ﻪﺑ ﺲﻳﺪﻴﻣرﺪﻴﭘا زا يا
9 و 10 ار ﻦﻴﺌﺗوﺮـﭘ
ﻦﻴﺌﺗوﺮـﭘ سﻮﻛﻮﻟ هرﺎﻤﺷ سﺎﺳاﺮﺑ ﻪﻛ دﻮﺷ ﻲﻣ ﻞﻣﺎﺷ يﺎـﻫ
رد ﻪﻛ يژﻮﻟﻮﻣﻮﻫ ناﺰﻴﻣ و قﻮﻓ 110
ﺶـﺨﺑ ﻪﻨﻴﻣآﺪﻴـﺳا
C
مﺎـﻧ ﻪـﺑ سﻮﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘﺳا رد ،ﺪﻧراد دﻮﺧ لﺎﻨﻴﻣﺮﺗ يﺎـﻫ
ScaA-I
ﻪـﺑ ﺲﻳﺪـﻴﻣرﺪﻴﭘا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘـﺳا رد و
مﺎﻧ يﺎﻫ
ScaA-J
ﺪـﻧﺪﻳدﺮﮔ يراﺬـﮔ مﺎﻧ
) 9
.(
ﻦﻴﺌﺗوﺮـﭘ يﺎـﻫ
هداﻮﻧﺎــــــﺧ ﻦــــــﻳا و سﻮــــــﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘــــــﺳا
ﻲـﮔﮋﻳو ﺲﻳﺪﻴﻣرﺪﻴﭘا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘﺳا رد ﻲﻬﺑﺎﺸـﻣ يﺎـﻫ
دﻮـﺟو ﻦﻴﻨﭽﻤﻫ و يژﻮﻟﻮﻣﻮﻫ ﺪﺻرد ،ﻚﻳﺮﺘﻜﻟاوﺰﻳا ﻪﻄﻘﻧ
ﺪـﻧراد ﺪﻴﺘﭙﭘ لﺎﻨﮕﻴﺳ .
ﺶﻴـﺑ ﻦﻴﺌﺗوﺮـﭘ رد يژﻮـﻟﻮﻣﻮﻫ ﻦﻳﺮـﺗ
يﺎﻫ و ScaB
ﺎﺑ ScaG
70
ﻢﻛ و ﺪﺻرد ﻮﻫ ﻦﻳﺮﺗ
يژﻮـﻟﻮﻣ
ﻦﻴﺌﺗوﺮــﭘ ﻦﻴــﺑ يﺎــﻫ
ﺎــﺑ ScaI
38 ﻪــﻧﻮﮔ ود ﻦﻴــﺑ رد ﺪــﺻرد
ﺖﺳا هﺪﺷ هﺪﻫﺎﺸﻣ
) 9
. (
تﺎـﻌﻟﺎﻄﻣ دﻮـﺟو مﺪـﻋ ﻪـﺑ ﻪﺟﻮﺗ ﺎﺑ
ﻪﻨﻣاد
نژ ﻪﻨﻴﻣز رد راد زا هدﺎﻔﺘـﺳا رﻮﻈﻨﻣ ﻪﺑ و هﺪﺷ دﺎﻳ يﺎﻫ
شور زا هدﺎﻔﺘﺳا ﻪﺑ ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ ،ﻲﻟﻮﻜﻟﻮﻣ ﻲﻳﺎﺳﺎﻨﺷ يﺎﻫ نژ
scaF
ﻪﻟوﺰﻳا ﻲﻳﺎﺳﺎﻨﺷ رد
ﻴﻨﻴﻟﺎﺑ يﺎـﻫ ﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘـﺳﺎ
س
ﻲﻣ سﻮﺋروا
ﻲﻣ ﻪﻛ دزادﺮﭘ ﺪﻧاﻮﺗ
دﺮـﻜﻳور ﻚـﻳ ناﻮـﻨﻋ ﻪﺑ
رد ﺪﻳﺪﺟ
ﺖـﻧﻮﻔﻋ رد نژﻮﺗﺎـﭘ ﻦـﻳا ﻲﻳﺎـﺳﺎﻨﺷ ﺖـﻬﺟ يﺎـﻫ
نآ ﻪﺑ طﻮﺑﺮﻣ دﺮﻴﮔ راﺮﻗ ﻪﺟﻮﺗ درﻮﻣ
.
ﺎﻫ شور و داﻮﻣ
يور ﻪـــﻌﻟﺎﻄﻣ ﻦـــﻳا 45
ﻪـــﻟوﺰﻳا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘـــﺳا
نﺎﺘــﺳرﺎﻤﻴﺑ زا هﺪــﺷ اﺪــﺟ سﻮــﺋروا مﻮــﻠﻋ هﺎﮕﺸــﻧاد يﺎــﻫ
ﺖـﻓﺮﮔ مﺎـﺠﻧا ناﺮـﻬﺗ ﻲﻜﺷﺰﭘ .
ﻲـﮔﮋﻳو ﻲﻳﺎﻴﻤﻴـﺷﻮﻴﺑ يﺎـﻫ
ﺖﻟﺎـﺳ لﻮﺘﻴﻧﺎﻣ ﻂﻴﺤﻣرد ﮓﻧر ﺮﻴﻴﻐﺗ و ﺪﺷر ﻞﻣﺎﺷ لواﺪﺘﻣ
،زﻻﺎــﺗﺎﻛ ،رﺎــﮔآ و زﻻﻮــﮔاﻮﻛ
DNase
ﺗ رﻮــﻈﻨﻣ ﻪــﺑ ﻳﺄ
ﺪــﻴ
ﻪﻟوﺰﻳا ﻦﻳا رد سﻮﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘﺳا يﺮﺘﻛﺎﺑ درﻮـﻣ ﺎﻫ
ﺖﻓﺮﮔ راﺮﻗ ﻲﺳرﺮﺑ .
مﻮﻧژ 45 ﻦﻴﻨﭽﻤﻫ و هﺪﺷ ﺪﻴﺋﺎﺗ ﻪﻟوﺰﻳا
سﻮـﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘـﺳا دراﺪﻧﺎﺘﺳا ﻪﻳﻮﺳ )
4 / 8325 ( زا
ﺖﺸﻛ يﺎﻫ 24 ﺖﻛﺮﺷ ﻞﻤﻌﻟارﻮﺘﺳد ﻖﺒﻃ و يﺮﺘﻛﺎﺑ ﻪﺘﻋﺎﺳ
ﺖﻴﻛ هﺪﻧزﺎﺳ )
نژﺎﻴﻛ - نﺎﻤﻟآ ( ﺪﻳدﺮﮔ جاﺮﺨﺘﺳا .
ﻪﺑ ﺲﭙﺳ
ﺮﻴﺜﻜﺗ رﻮﻈﻨﻣ نژ
هدﺎﻔﺘـﺳا ﻲﺻﺎﺼـﺘﺧا يﺎﻫﺮﻤﻳاﺮﭘ زاscaF
ﺪﺷ . ﺖﺳا ﺮﻳز حﺮﺷ ﻪﺑ ﺎﻫﺮﻤﻳاﺮﭘ ﻦﻳا ﻲﻟاﻮﺗ :
scaF1:GCGCGCGCTAGCATGTATACGAATGATAGCA AAACATTAG
scaF2:GCGCGCCTCGAGATGGATGTAATTATATGAT GAAACTTCTG
ﺶﻨــــﻛاو
(PCR)
)
(PCRPolymrase Chain
Reaction
ﻞﻣﺎﺷ 1 زاﺮـﺘﻴﻟ وﺮـﻜﻴﻣ ،هﺪـﺷ جاﺮﺨﺘـﺳاDNA
5 / 0
زا ﺮﺘﻴﻟوﺮﻜﻴﻣ
ﺎـﻫﺮﻤﻳاﺮﭘزا ﻚـﻳ ﺮـﻫ )
10pM
( و 5 / 12
ﺮــﺘﻴﻟوﺮﻜﻴﻣ زا
Mastermix 2x
)
Cinagene, Cat.No:
PR8250C
( و دﻮﺑ رد ﺮـﻄﻘﻣ بآ ندﺮـﻛ ﻪﻓﺎـﺿا ﺎﺑ ﺖﻳﺎﻬﻧ
ﻲﻳﺎﻬﻧ ﻢﺠﺣ 25
ﻜﻴﻣ
ﺪـﻣآ ﺖﺳد ﻪﺑ ﺮﺘﻴﻟوﺮ .
طﻮـﻠﺨﻣ ﺲﭙـﺳ
ﺶﻨـﻛاو
PCR
ﺮﻠﻜﻳﺎـﺳﻮﻣﺮﺗ هﺎﮕﺘـﺳد رد
(Sensoquest,
Labcycler, Germany)
ﺖﻓﺮﮔ راﺮﻗ و
ﺮـﻈﻧ درﻮـﻣ ﻪﻌﻄﻗ
ﺪــﺷ ﺮــﻴﺜﻜﺗ ﺮــﻳز ﻪــﻣﺎﻧﺮﺑ ﻖــﺒﻃ :
ﻪــﻴﻟوا يﺎــﻣد 94
ﻪــﺟرد
ﻲﺘﻧﺎﺳ تﺪـﻣ ﻪـﺑ داﺮﮔ 5
ﺲﭙـﺳ ،ﻪـﻘﻴﻗد 35
ﻲﻳﺎـﻣد ﻞﻜﻴـﺳ
يﺎﻣد ﻞﻣﺎﺷ 98
تﺪـﻣ ﻪـﺑ داﺮـﮔ ﻲﺘﻧﺎـﺳ ﻪـﺟرد 10
،ﻪـﻴﻧﺎﺛ
يﺎﻣد 60
ﻲﺘﻧﺎﺳ ﻪﺟرد تﺪﻣ ﻪﺑ داﺮﮔ
30 يﺎـﻣد و ﻪﻴﻧﺎﺛ 72
ﻲﺘﻧﺎﺳ ﻪﺟرد تﺪﻣ ﻪﺑ داﺮﮔ
1 يﺎـﻣد ﺖﻳﺎﻬﻧ رد و ﻪﻘﻴﻗد 72
ﻲﺘﻧﺎﺳ ﻪﺟرد تﺪﻣ ﻪﺑ داﺮﮔ
5 ﻪﻘﻴﻗد . مﺎﺠﻧا زا ﺲﭘ ﻪﺑPCR
تﻻﻮﺼــﺤﻣ ،هﺪــﺷ ﺮــﻴﺜﻜﺗ تﺎــﻌﻄﻗ ﻲــﺳرﺮﺑ رﻮــﻈﻨﻣ
PCR
ﻂــﺳﻮﺗ
KBC power load
زرﺎــﮔآ لژ يور 1
ﺪــﺻرد
رﻮﻧ ﺮﻳز و هﺪﺷ زرﻮﻓوﺮﺘﻜﻟا ﺶﻔﻨﺑاﺮﻓ
راﺮـﻗ هﺪﻫﺎﺸـﻣ درﻮـﻣ
ﺪﻨﺘﻓﺮﮔ . ،ﺎـﻫ ﻪـﻟوﺰﻳا زا ﻲـﻜﻳ رد ﺰﻳﺎﺳ فﻼﺘﺧا ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ
نژ ﺮـﻴﺜﻜﺗ رﺎـﺑ ﻪﺳ
ود و ﺖـﻓﺮﮔ ترﻮـﺻ نآ ياﺮـﺑ scaF
ﺖﻛﺮـﺷ ﻪـﺑ ﻲﻟاﻮـﺗ ﻦﻴـﻴﻌﺗ ياﺮـﺑ توﺎـﻔﺘﻣ ﺰﻳﺎـﺳ ﺎـﺑ ﻪﻟوﺰﻳا ﺪــﺷ هدﺎﺘــﺳﺮﻓ ﺖﺴــﻳزﻮﭘﺎﻜﺗ .
ﻪﺘــﺷر ﺮــﻫ ﻲﻟاﻮــﺗ ﺎــﺑ DNA
ﺰـﻓا مﺮﻧ ﻂﺳﻮﺗ و ﻪﻃﻮﺑﺮﻣ ماﺮﮔﻮﺗﺎﻣوﺮﻛ زا هدﺎﻔﺘﺳا را
gene
runner
ﺪﺷ ﺰﻴﻟﺎﻧآ .
ﺎﻫ ﻪﺘﻓﺎﻳ
ﻪﻤﻫ 45 هﺪـﺷ ﻲﺳرﺮﺑ سﻮﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘﺳا ﻪﻟوﺰﻳا
شور ﻂﺳﻮﺗ ﻲﻳﺎﻴﻤﻴﺷﻮﻴﺑ يﺎﻫ
ﺪﻨﺘﻓﺮﮔ راﺮﻗ ﺪﻴﺋﺎﺗ درﻮﻣ .
ﻪـﻟوﺰﻳا 5PCR لﻮﺼـﺤﻣ ﺪﺻرد 1 لژزرﺎﮔآ زرﻮﻓوﺮﺘﻜﻟا: 1 هرﺎﻤﺷﺮﻳﻮﺼﺗ
سﻮــــــــﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘــــــــﺳا :
نﻮﺘــــــــﺳ 1 : ﺮﻛرﺎــــــــﻣ 1kb
)
(Vivantis,Cat.No:NL1411kb
. نﻮﺘــــﺳ 6 - 2 : لﻮﺼــــﺤﻣ نژ PCR
scaF ) 894bp(
رد 5 سﻮﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘﺳا ﻪﻟوﺰﻳا .
نﻮﺘﺳ 7 : لﺮﺘﻨﻛ
ﺖﺒﺜﻣ ) سﻮـﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘـﺳا دراﺪﻧﺎﺘـﺳا ﻪﻳﻮـﺳ 4
/ 8325 .(
نﻮﺘـﺳ 8 :
ﻲﻔﻨﻣ لﺮﺘﻨﻛ
.
زا ﻞـــﺻﺎﺣ ﺞﻳﺎـــﺘﻧ
زرﺎـــﮔآ لژ و PCR
1 ﺪـــﺻرد
زرﻮﻓوﺮﺘﻜﻟا )
هرﺎﻤﺷ ﺮﻳﻮﺼﺗ 1
( ﻪـﻤﻫ رد ﻪـﻛ داد نﺎﺸـﻧ 45
،ﻪﻟوﺰﻳا دوﺪﺣ يﺪﻧﺎﺑ 894
رد ﻲـﻟو دراد رﻮﻀﺣ زﺎﺑ ﺖﻔﺟ
لﻮﺼﺤﻣ ود دوﺪﺣ ﺎﺑ توﺎﻔﺘﻣ هزاﺪﻧا ود PCR
100 ﺖﻔﺟ
ﺪﻳدﺮﮔ هﺪﻫﺎﺸﻣ زﺎﺑ .
ﻲﻟاﻮﺗ ﻦﻴﻴﻌﺗ تﺎﺸﻳﺎﻣزآ ﺞﻳﺎﺘﻧ و scaF
هزاﺪــﻧا فﻼﺘــﺧا ﭻﻴــﻫ ﻲﻟاﻮــﺗ ﻦﻴــﻴﻌﺗ ﺪــﻧدﺮﻜﻧ ﺪــﻴﺋﺎﺗ ار يا
.
ﻪـﺑ ﻲـﻨﻴﺌﺗوﺮﭘ نژ ﻦﻳا ﻪﻛ داد نﺎﺸﻧ ﻚﻴﺗﺎﻣرﻮﻔﻧاﻮﻴﺑ ﻲﺳرﺮﺑ ﻲﻟﻮﻜﻟﻮﻣ نزو 33
ﻛ ياراد و هدﺮـﻛ ﺪﻛ ار نﻮﺘﻟادﻮﻠﻴ 297
ﻚــﻳﺮﺘﻜﻟاوﺰﻳا ﻪــﻄﻘﻧ ﺎــﺑ ﻪﻨﻴﻣآﺪﻴــﺳا 3
/ 6 ﻲــﻣ ﺪــﺷﺎﺑ . ﻦــﻳا
ﺶـﺨﺑ رد ﻦﻴﺌﺗوﺮﭘ
لﺎﻨﮕﻴـﺳ ﻚـﻳ ياراد دﻮـﺧ لﺎـﻨﻴﻣﺮﺗ N
رد و هدﻮــــﺑ ﺪــــﻴﺘﭙﭘ يﺎــــﻬﺘﻧا
ﻚــــﻳ ياراد لﺎــــﻨﻴﻣﺮﺗ C
CHAP domain
ﻲــﻣ
ﻪﻳﻮــﺳ ﻪــﻤﻫ رد ﻪــﻛ ﺪــﺷﺎﺑ يﺎــﻫ
ﺖﺳا هﺪﺷ ﻆﻔﺣ ﻞﻣﺎﻛ رﻮﻃ ﻪﺑ سﻮﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘﺳا .
ﺚﺤﺑ
لﻮﺌﺴﻣ ﻲﻠﺻا نژﻮﺗﺎﭘ ﻚﻳ سﻮﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘﺳا ﻪـــﻌﻣﺎﺟ زا هﺪـــﺷ ﺐﺴـــﻛ و ﻲﻧﺎﺘـــﺳرﺎﻤﻴﺑ يﺎـــﻫ ﺖـــﻧﻮﻔﻋ
ﻲﻣ ﺪﺷﺎﺑ
) 10
سﻮـﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘـﺳا ﻊﻳﺮـﺳ ﺺﻴﺨﺸﺗ (.
ﻞــﺑﺎﻗ ﺮﻴﺛﺎـﺗ ﺎـﻫ ﻪـﻨﻳﺰﻫ ﺶﻫﺎـﻛ و يرﺎـﻤﻴﺑ دﻮـﺒﻬﺑ ﺪـﻧور رد ﻲﻬﺟﻮﺗ دراد
) 11
.(
ﻪـﻋﻮﻤﺠﻣ سﻮﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘﺳا يا
نژ زا ﻪـﻛ ﺪـﻨﻛ ﻲـﻣ ﻞـﻤﺣ ار يا هﺪﺷ ﺖﻇﺎﻔﺣ رﺎﻴﺴﺑ يﺎﻫ
ﻲﻣ
نژ ﻦﻳا زا ناﻮﺗ نژﻮﺗﺎﭘ ﻦﻳا ﻊﻳﺮﺳ ﺺﻴﺨﺸﺗ ﺖﻬﺟ رد ﺎﻫ
دﺮﻛ هدﺎﻔﺘﺳا .
هﺪـﺷ ﺖـﻇﺎﻔﺣ نژ ،ﻲﻟﻮـﻜﻟﻮﻣ تﺎـﻌﻟﺎﻄﻣ رد femA
ـﺑ سﻮـﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘﺳا ﻲﻳﺎﺳﺎﻨﺷ ياﺮﺑ ﻪ
رﺎـﻛ
ﻲــﻣ ﻪــﺘﻓﺮﮔ دﻮــﺷ
) 12 ، 13
.(
ًﺎﻨﻤــﺿ ياﺮــﺑ سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘــﺳا
ﻲﺑﺎــﻳدر زا ﻦﻴﻠﻴــﺳ ﻲــﺘﻣ ﻪــﺑ موﺎــﻘﻣ سﻮــﺋروا نژ
mecA
ﻲﻣ هدﺎﻔﺘﺳا ﻲﺻﺎﺼـﺘﺧا،ﻊﻳﺮﺳ ﻲﻳﺎﺳﺎﻨﺷ شور ﻦﻳا ﻪﻛ دﻮﺷ
ﻲــﻣ سﺎﺴـﺣ و ﺪــﺷﺎﺑ
) 15،14
شور .(
ﻲﻟﻮــﻜﻟﻮﻣ ﺮــﮕﻳد يﺎـﻫ
نژ ياﺮــﺑ ﺮــﻴﻈﻧ ﻲﻳﺎــﻫ
)pvl 17،16
،(
و sea )seb 19،18
رد (
ﻲﻳﺎﺳﺎﻨﺷ ﺖﻬﺟ سﻮﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘﺳا
ﺑ ﻪ ﻪـﺘﻓﺮﮔ رﺎـﻛ
ﺖﺳا هﺪﺷ .
رﻮﻈﻨﻣ ﻪﺑ ﻲﻟﻮﻜﻟﻮﻣ تﺎﻌﻟﺎﻄﻣ رد ،ﻦﻳا ﺮﺑ هوﻼﻋ
نژ زا ﺲﻳﺪــﻴﻣرﺪﻴﭘا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘــﺳا ﻊﻳﺮــﺳ ﻲﻳﺎــﺳﺎﻨﺷ
femA-SE
دﻮﺷ ﻲﻣ هدﺎﻔﺘﺳا
) 21،20
ﻲـﻧژ عﻮﻨﺗ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ .(
نژ زا هدﺎﻔﺘــﺳا ﺖــﻴﻓﺮﻇ سﻮــﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘــﺳا يﺎــﻫ
ﺪﺷﺎﺑ ﻲﻣ ﺮﺴﻴﻣ ﺮﮕﻳد .
ﻪﻳﻮﺳ ﺮﺑ هوﻼﻋ ﻲـﺘﻧآ ﻪﺑ سﺎﺴﺣ يﺎﻫ
ﺑ ﻪﻳﻮـﺳ هد ﻞﻗاﺪـﺣ ،ﻪﻧﻮﮔ ﻦﻳا رد هﺪﺷ ﻲﻟاﻮﺗ ﻦﻴﻴﻌﺗ ﻚﻴﺗﻮﻴ
ـﺑ سﻮـﺋروا سﻮﻛﻮﻠﻴﻓﺎﺘﺳا ﻪ
ﻲﻟاﻮـﺗ ﻦﻴـﻴﻌﺗ ﻞـﻣﺎﻛ ترﻮـﺻ
هﺪﺷ ﺪﻧا . نژ زا ﻲـﺘﺣ ﻲـﺘﺣار ﻪـﺑ هزوﺮـﻣا
vanA
ﻲـﻣ ناﻮـﺗ
ﻪﻟوﺰﻳا يﺮﮕﻟﺎﺑﺮﻏ ﺖﻬﺟ هﺮـﻬﺑ ﻦﻴﺴـﻳﺎﻣﻮﻜﻧو ﻪـﺑ موﺎﻘﻣ يﺎﻫ
ﺖﻓﺮﮔ . شور
نژ سﺎـﺳاﺮﺑ ﻲﻟﻮـﻜﻟﻮﻣ ﻲﻳﺎـﺳﺎﻨﺷ يﺎﻫ يﺎـﻫ
،هﺪــﺷ ﺮــﻛذ ﺪــﻨﻣزﺎﻴﻧ
نﺎــﻣز ﻢــﻛ ﺎــﺑ ﻪﺴــﻳﺎﻘﻣ رد ار يﺮــﺗ
شور
ﻲﭙﻴﺗﻮــﻨﻓ لواﺪــﺘﻣ يﺎــﻫ ﺖــﺳا
. نژ زا ﻪــﭼﺮﮔا
pvl
ﻲﻣ ﺪﻧاﻮﺗ د ر
ﻪﻟوﺰﻳا ﻲﻳﺎﺳﺎﻨﺷ ﺖﻬﺟ سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘـﺳا يﺎﻫ
ﺖﻧﻮﻔﻋ رد سﻮﺋروا
يﺎـﻫ نژ و مﺮـﻧ ﺖـﻓﺎﺑ و ﻲﺘـﺳﻮﭘ يﺎﻫ
نژ ﻲﻫﺎﮕﺸﻳﺎﻣزآ و ﻲﻜﻴﺗﺎﻣرﻮﻔﻧاﻮﻴﺑ ﻲﺳرﺮﺑ
نارﺪﻧزﺎﻣ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻪﻠﺠﻣ
هرود
مرﺎﻬﭼ و ﺖﺴﻴﺑ
، هرﺎﻤﺷ 116 رﻮﻳﺮﻬﺷ ،
1393 56
و sea
seb
ﺖﻴﻣﻮﻤﺴﻣ رد ﻲـﻟو دﺮـﻛ هدﺎﻔﺘـﺳا ﻲﻳاﺬﻏ يﺎﻫ
ﻲﻤﻧ ﺎـﺑ ار سﻮـﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘﺳا يﺎﻫ ﻪﻳﻮﺳ ﻪﻤﻫ ناﻮﺗ
نآ
دﻮﻤﻧ ﻲﺑﺎﻳدر ﺎﻫ .
ﻲﻟﺎﺣ رد نژ زا هدﺎﻔﺘﺳا ﺎﺑ ﻪﻛ ﻪـﻧscaF
ﻲــﻣ ﺎــﻬﻨﺗ ﻪﻳﻮــﺳ ناﻮــﺗ
ﻪــﻟوﺰﻳا ﺮــﻫ ﻪــﻜﻠﺑ صﺎــﺧ يﺎــﻫ
ﻪﻧﻮﻤﻧ رد ار سﻮﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘﺳا درﻮـﻣ ﻲﻨﻴﻟﺎـﺑ يﺎـﻫ
داد راﺮﻗ ﻲﺳرﺮﺑ .
نژ زا هدﺎﻔﺘﺳا ﺎﺑ ﻲﻤﻧscaF
ﺖـﻧﻮﻔﻋ ناﻮﺗ
ﻮﺳ زا ﻲﺷﺎﻧ ار
دروآ رﺎﻤـﺷ ﻪﺑ ﻦﻴﻠﻴﺳ ﻲﺘﻣ ﻪﺑ موﺎﻘﻣ يﺎﻫ ﻪﻳ .
ﻲـﻣ مﺎـﻬﺑا ﻦـﻳا ﻊـﻓر ﺖﻬﺟ ناﻮـﺗ
نژ ﻲـﺳرﺮﺑ ﻪـﺑ نﺎـﻣﺰﻤﻫ
ﺖﺧادﺮﭘ mecA
. ﺖـﺳا نآ زا ﻲﻛﺎﺣ هﺪﺷ مﺎﺠﻧا تﺎﻌﻟﺎﻄﻣ
ﻦﻴﺌﺗوﺮﭙــﺳﻮﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘــﺳا يﺮﺘﻛﺎــﺑ رد ﻪــﻛ يﺎــﻫ
هداﻮﻧﺎﺧ
يﺎﻀـﻋا و هدﻮﺑ هﺪﺷ ﺖﻇﺎﻔﺣ ﻲﻳﻻﺎﺑ ﺪﺣ رد Sca
روا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘﺳا رد هداﻮﻧﺎﺧ ﻦﻳا ﺪﻗﺎﻓ سﻮﺋ
LPTXG
ﻪـﺑ لﺎـﺣ ﻦـﻳا ﺎـﺑ ،ﺪﻨﺷﺎﺑ ﻲﻣ دﻮﺧ يا ﻪﻨﻴﻣآﺪﻴﺳا ﻒﻳدر رد يﺎـــﻬﺘﻧا رد ﺪـــﻴﺘﭙﭘ لﺎﻨﮕﻴـــﺳ دﻮـــﺟو ﻞـــﻴﻟد لﺎـــﻨﻴﻣﺮﺗ N
ﻦﻴﺌﺗوﺮﭙﻨﻳا
،ﺎﻫ ﻲﺤﺷﺮﺗ ﺖﺳا ﻦﻜﻤﻣ هداﻮﻧﺎﺧ ﻦﻳﺎﺋﺎﻀﻋا ﻪﻤﻫ
ﺪﻧﻮـﺷ ﻪﺿﺮﻋ يﺮﺘﻛﺎﺒﻟﻮﻠﺳ ﺢﻄﺳ رد ﺎﻳ و
) 9
.(
هداﻮﻧﺎـﺧ ﻦـﻳا سﻮﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘﺳا ندﺮﻛ هﺰﻴﻧﻮﻠﻛ رد ﻲﺗﺎﻴﺣ ﺶﻘﻧ
ور
دراد نﺎﺑﺰﻴﻣ لﻮﻠﺳ ي
) 9
. (
ﺎﻣ ﻪﻌﻟﺎﻄﻣ ﻦﻳا رد نژ
ارscaF
رد 45 شور ﺎــﺑ ﻪــﻟوﺰﻳا
ﺞﻳﺎــﺘﻧ ﻪــﻛ ﻢﻳدﺮــﻛ ﻲــﺳرﺮﺑPCR
زا ﻞﺻﺎﺣ يﺎـﻫ ﻪﻟوﺰﻳا ﻪﻤﻫ رد نژ ﻦﻳا ﻪﻛ داد نﺎﺸﻧ PCR
هدرﻮﺨﻨﺘـﺳد ترﻮـﺻ ﻪـﺑ سﻮﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘﺳا ﻞـﺑﺎﻗ
ﻲﻣ هﺪﻫﺎﺸﻣ ﺪـﺷﺎﺑ
. نآ زا ﻪـﻛ ﻲﻳﺎـﺟ نژ
هداﻮﻧﺎـﺧ زاscaF
ﻲﻧژ Sca
ﻪﻳﻮﺳ ﻪﻤﻫ رد ، سﻮـﺋروا سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘـﺳا يﺎـﻫ
ﻲــــﻣ هﺪــــﺷ ﺖــــﻇﺎﻔﺣ رد نژ ﻦــــﻳا ﻪﺑﺎﺸــــﻣ و ﺪــــﺷﺎﺑ
ﻛﻮﻛﻮﻠﻴﻓﺎﺘـــﺳا ﻪـــﻧﻮﮔ ﺮﻳﺎـــﺳ و ﺲﻳﺪـــﻴﻣرﺪﻴﭘا سﻮ
يﺎـــﻫ
ﻲﻣ ﺖﺳا هﺪﺸﻧ ﺖﻓﺎﻳ ﻲﻳﺎﻳﺮﺘﻛﺎﺑ ﺎـﺑ نژ ﻦـﻳا ﻲﺑﺎﻳدر زا ناﻮﺗ
شور سﻮﻛﻮﻛﻮﻠﻴﻓﺎﺘـﺳا ﻲﻳﺎﺳﺎﻨﺷ رﻮﻈﻨﻣ ﻪﺑ ﻲﻟﻮﻜﻟﻮﻣ يﺎﻫ
ﺖﻧﻮﻔﻋ رد سﻮﺋروا دﺮﻛ هدﺎﻔﺘﺳا ﺎﻫ
.
ﮕﺳﺎﭙﺳ ﺰ
يرا
ﻲﺸـﻫوﮋﭘ ﺖﻧوﺎﻌﻣ يرﺎﻜﻤﻫ و ﻲﻧﺎﺒﻴﺘﺸﭘ ﺎﺑ ﻖﻴﻘﺤﺗ ﻦﻳا ﺖﺳا ﻪﺘﻓﺮﮔ ترﻮﺻ ناﺮﻬﺗ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد .
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