生命化学研究レター No.28 (2008 October)

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Enz NH₂ (a)

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domain +

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CHNHQKATCRRMRSRETSVKK (rpf1368) Sequences of peptides

CXXXXXXXC XXXXXXX XXXXXXXXXXXX

Selection

Selection Libraries (first generation)

Libraries (third generation)

Sequences of peptides that bound to the diketone

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Selection Libraries (second generation)

Sequences of peptides that bound to the diketone NPLGAKLAGVFWWGQTPGG

R

O O

21-mer peptide Lys

NH

protein of interest

R O

R = Labeling compounds 21-mer peptide

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protein of interest NH₂

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HN O H

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HN O

O OH

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weakly fluorescent highly fluorescent

H CO₂Et O

R NHPMP H CO2Et

N H

O

R

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H CO2H

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syn-selective CO₂H H CO₂Et

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R NHPMP H CO2Et

N R¹

O

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anti-selective +

NH

syn-selective CO2H R¹ CO₂Et

O

R NHPMP

NH CO2H

3 2 3 2

(2S,3S) Ketones (2S,3R)

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1) Tanaka, F.; Fuller, R.; Shim, H.; Lerner, R. A.; Barbas, C. F., III. J. Mol. Biol. 2004, 335, 1007-1018.

2) Tanaka, F.; Fuller, R.; Barbas, C. F., III. Biochemistry 2005, 44, 7583-7592.

3) Tanaka, F.; Barbas, C. F., III. J. Am. Chem. Soc. 2002, 124, 3510-3511.

4) Tanaka, F.; Fuller, R.; Asawapornmongkol, L.; Warsinke, A.; Gobuty, S.; Barbas, C. F., III. Bioconjugate Chem. 2007, 18, 1318-1324.

5) Tanaka, F.; Thayumanavan, R.; Barbas, C. F., III. J. Am. Chem. Soc. 2003, 125, 8523-8528.

6) Tanaka, F.; Thayumanavan, R.; Mase, N.; Barbas, C. F., III. Tetrahedron Lett. 2004, 45, 325-328.

7) (a) Mase, N.; Tanaka, F.; Barbas, C. F., III. Org. Lett. 2003, 5, 4369-4372. (b) Mase, N.; Tanaka, F.; Barbas, C. F., III.

Angew. Chem., Int. Ed. 2004, 43, 2420-2423. (c) Mase, N.; Thayumanavan, R.; Tanaka, F.; Barbas, C. F., III. Org. Lett.

2004, 6, 2527-2530.

8) Tanaka, F.; Mase, N.; Barbas, C. F., III. J. Am. Chem. Soc. 2004, 126, 3692-3693.

9) (a) Mitsumori, S.; Zhang, H.; Cheong, P. H.-C.; Houk, K. N.; Tanaka, F.; Barbas, C. F., III. J. Am. Chem. Soc. 2006, 128, 1040-1041. (b) Zhang, H.; Mifsud, M.; Tanaka, F.; Barbas, C. F., III. J. Am. Chem. Soc. 2006, 128, 9630-9631. (c) Zhang, H.; Mitsumori, S.; Utsumi, N.; Imai, M.; Garcia-Delgado, N.; Mifsud, M.; Albertshofer, K.; Cheong, P. H.-Y.; Houk, K. N.;

Tanaka, F.; Barbas, C. F., III. J. Am. Chem. Soc. 2008, 130, 875-886.

10) Tanaka, F. The Chemical Record 2005, 5, 276-285.

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J. Am. Chem. Soc., 130, 7798-7799 (2008).

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K. M. Wilcoxen, L. J. Leman, D. A. Weinberger, Z.-Z. Huang, and M. R. Ghadiri, J. Am. Chem. Soc., 129, 748-749 (2007).

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J. Huang, A. Koide, K. Makabe, and S. Koide, Proc. Natl. Acad. Sci. USA, 105, 6578-6583 (2008).

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F. Fan, B. F. Binkowski, B. L. Butler, P. F. Stecha, M. K. Lewis, and K. V. Wood, ACS Chem. Biol., 3, 346-351 (2008).

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D. Röthlisberger, O. Khersonsky, A. M. Wollacott, L. Jiang, J. DeChancie, J. Betker, J. L. Gallaher, E. A.

Althoff, A. Zanghellini, O. Dym, S. Albeck, K. N. Houk, D. S. Tawfik and D. Baker, Nature, 453, 190-195 (2008).

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Papp-Szabo, D. W. Lowman, T. D. Power, M. F. Wempe, and D. L. Williams, J. Pharmacol. Exp. Ther., 325, 115-123 (2008).

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[1] Luisi, P.L., Walde, P. Giant Vesicles; John Wiley & Sons Ltd: Chichester (2000).

[2] Angelova, M.I., Soléau, S., Méléard, Ph., Faucon, J.F., Bothorel, P. Progr. Colloid Polym. Sci., 89, 127-131 (1992).

[3] Magome, N., Takemura, T., Yoshikawa, K. Chem. Lett., 26, 205-206 (1997).

[4] Yamada, N.L., Hishida, M., Seto, H., Tsumoto, K., Yoshimura T. Europhys. Lett., 80, 48002 (2007).

[5] Yamashita, Y., Oka, M., Tanaka, T., Yamazaki, M. Biochim. Biophys. Acta, 1561, 129-134 (2002).

[6] Akashi, K., Miyata, H., Itoh, H., Kinoshita, K.Jr. Biophys. J., 74, 2973-2982 (1998).

[7] Yamada, A., Yamanaka, T., Hamada, T., Hase, M., Yoshikawa, K., Baigl, D. Langmuir, 22, 9824-9828 (2006).

[8] Pautot, S., Frisken, B.J., Weitz, D.A. Proc. Natl. Acad. Sci. U.S.A., 100, 10718-10721 (2003).

[9] Evans, E., Rawicz, W. Phys. Rev. Lett., 64, 2094-2097 (1990).

[10] Hamada, T., Miura, Y., Ishii, K., Araki, S., Yoshikawa, K., Vestergaard, M., Takagi, M. J. Phys. Chem. B, 111, 10853-10857 (2007).

[11] Nomura, S.M., Tsumoto, K., Hamada, T., Akiyoshi, K., Nakatani, Y., Yoshikawa, K. ChemBioChem, 4, 1172-1175 (2003).

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Am. Chem. Soc., 128, 15948-15949 (2006); Cohen, E., et al., Science, 313, 1604-1610 (2006); Bosco, D.A., et al., Nat. Chem. Biol., 2, 249-253 (2006); Fowler, D.M., et al., PLoS Biol., 4, e6, (2006)§¦

2) Zhang, Q. et al., Proc. Natl. Acad. Sci. U.S.A., 101, 4752-4757 (2004); Bieschke, J., et al., Acc. Chem.

Res., 39, 611-619 (2006) §¦

3) http://www.geocities.jp/japan_scripps/

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