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2014 R&D Highlights - Philippine Rice Research Institute

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Nipa palm (Nypa fruticans Wurmb.) has been identified as an important source of biofuel in the Philippines due to its ability to produce a large amount of sap that can be converted to alcohol. Percentage decontamination of young leaf explants sterilized with different sterilants after 10 weeks of incubation in the dark.

Figure 1. Planting materials utilized in the study; a) Fruiting head of nipa; b) Mature seeds detached from the fruit stem; c) Cut seeds showing the  hardened endosperm; d)
Figure 1. Planting materials utilized in the study; a) Fruiting head of nipa; b) Mature seeds detached from the fruit stem; c) Cut seeds showing the hardened endosperm; d)

Collection and Genetic Diversity Analysis of Nipa Palm (Nypa Fruiticans Wurmb., Aracaceae) Germplasm

An overall genetic diversity of 0.61, indicating a relatively adequate level of genetic variation among the accessions, was identified. SSR markers proved useful for genetic diversity analysis as they successfully distinguished polymorphisms among nipa palm assemblages.

Figure 5. Nipa germplasm collection at PhilRice Los Banos A) Eight-month  old nipa plant planted in plastic pails B) Newly planted collections C) Newly
Figure 5. Nipa germplasm collection at PhilRice Los Banos A) Eight-month old nipa plant planted in plastic pails B) Newly planted collections C) Newly

Collection and Genetic Diversity Analysis of Nipa Palm (Nypa Fruiticans Wurmb., Aracaceae) Germplasm

Major components such as the head, infeed conveyor, thresher, cleaner, blower, screw conveyors, platform, undercarriage and undercarriage were manufactured at Rice Engineering and Mechanization Division (REMD) machine shop. Some parts such as gears, undercarriage rollers and hydraulics were outsourced from the members of Metalworking Industries Association of the Philippines, Inc.

Figure 8. 3D model of the improved 1.3m rice combine  harvester prototype.
Figure 8. 3D model of the improved 1.3m rice combine harvester prototype.

Development and Pilot Testing of a Local Riding-Type Transplanter

The tests proved the suitability of the local riding-type transplanter in different depths of rice field conditions with a field capacity of 1.25 ha/day in the first gear setting to 2.16 ha/day in the second gear setting. the 3rd. To determine its acceptability, the commercial model will undergo viability test and pilot testing in the three main islands of the country (Luzon, Visayas and Mindanao) especially in the rice producing areas.

Figure 11. The first prototype of transplanter.
Figure 11. The first prototype of transplanter.

Development of a locally adapted and manufacture riding- type precision seeder

The manufacture of 8 units of seed metering unit and container was contracted by Rollmaster Machinery. Reducing the weight of the seed metering unit is necessary to reduce the overall weight as well as the cost.

Figure 13. The hydraulic system.
Figure 13. The hydraulic system.

Development of Elite Heat Tolerance Rice Using the Common Germplasm and Analysis of QTLS Related to Heat

One hundred lines of a backcrossed population (BC4F4) with sterility (phenotype) and marker (genotype) data during planting in the 2014 dry season and phenotypically selected using marker-assisted selection data from the previous season. Number of lines selected in the field based on phenotype (percent sterility) and genotype (molecular data).

Table 10. Summary of selected heat-tolerant rice lines during the 2014 dry  season in Southern Cagayan Research Center, Cagayan and PhilRice-CES.
Table 10. Summary of selected heat-tolerant rice lines during the 2014 dry season in Southern Cagayan Research Center, Cagayan and PhilRice-CES.

Construction of Epidemiology Information Interchange System for Migratory Disease and Insect Pests in Asia Region

During the field sampling, spiders, coccinelids, mirids and tiger beetles were commonly observed in the field. These observed high populations of RPH monitored using sticky trap coincided with the reproductive to maturation phases of rice plants in the field.

Increasing productivity of direct seeded rice areas by incorporating genes for tolerance of anaerobic conditions

This study attempts to introduce anaerobic germination tolerance with submergence tolerance genes, Sub1 + AG1 (Parent: Ciherang Sub1+AG1) into NSIC Rc222. F1 seeds of Ciherang (Sub1+ Ag1) X NSIC Rc222 with anaerobic germination tolerance were planted in the greenhouse in 2014DS.

Figure 25. Establishment of anaerobic germination and crossing activities in  the glasshouse during 2014 DS.
Figure 25. Establishment of anaerobic germination and crossing activities in the glasshouse during 2014 DS.

Expanded GXE Experiments in Different Agro-Ecologies in Support of Bangladesh and Eastern India High-Zinc

Yield and other agronomic characteristics of test entries in the Pre-NCT Trial, PhilRice-CES, 2014DS. Yield and other agronomic characteristics of test entries in the Pre-NCT trial, PhilRice-Isabela, 2014DS.

Table 17. Yield and other agronimic characteristics of the test entries in the  replicated field trial, PhilRice-CES, 4DS.
Table 17. Yield and other agronimic characteristics of the test entries in the replicated field trial, PhilRice-CES, 4DS.

Ecological Engineering Approach for Pest Management

Population of predators, parasitoids and species richness were higher in fields close to flowering plants compared to fields without flowering plants. This can be attributed to the presence of flowering plants that serve as refuge and food source such as honey, nectar and pollen for these beneficial organisms. In most cases, they must fly long distances to forage for honey and nectar from flowering plants.

Therefore, it is vital for their survival and effectiveness as a biological control agent to plant these flowering plants to take advantage of their function in the rice ecosystem.

Hybrid Rice Development Consortium Multi-Location Replicated Yield Trial

Conservation of the rich communities of these beneficial organisms in the rice ecosystem is an important component for a sustainable integrated pest management (IPM) in rice. Since rice is a monoculture crop with low habitat biodiversity for beneficial organisms such as parasitoids and some species of predators, they are the most affected group as they need food that is not available in the rice field. Planting flowering plants improves habitat diversity, resulting in higher arthropod richness, higher abundance of predators and parasitoids in rice paddies and, in effect, low vulnerability to pest outbreaks.

An item developed by PhilRice, PR35711H, was also included in the trial and was given the code HRDC1435.

Table 25. Yield and trait performance of hybrids in 2014DS at Science City  of Muñoz, Nueva Ecija, Philippines.
Table 25. Yield and trait performance of hybrids in 2014DS at Science City of Muñoz, Nueva Ecija, Philippines.

Increasing Farmers’ Access to High-Quality Rice Seeds through Efficient Seed Production Systems

These educational and campaign materials can serve as a bible for the farmers every time they grow rice for seed. A baseline survey of farmer beneficiaries prior to the project implementation was conducted to establish key impact indicators that will be used as a basis for measuring the outcome of the project interventions. Seasonal monitoring survey after 2013 DS and 2014 WS harvest was carried out to evaluate the results of the project interventions, especially the impact of the 2kg seeds in facilitating adoption of HQS and providing training to farmers on improving and maintaining rice seed quality.

Green Super Rice (GSR) for Resource-poor Farmers of the Philippines

4, 2014, GSR IRI 1 emerged as the most preferred with farmers commenting on its drought resistance, higher yield and adaptation than the local check in the area. In the same season, more farmers in the adjacent municipalities in Gerona, Camiling, Moncada and Pura, Tarlac and Nampicuan, Nueva Ecija were able to access the seeds for planting in the next WS. In the same area, the Rice Crop Manager (RCM) application software was introduced to farmers in Lutayan, Sultan Kudarat as a decision tool to improve farm productivity.

List of recipient farmers for GSR lines in techno-demo seed production trials, 2013.

Table 27.  Profile of the MET entries including GSR lines, 2014.
Table 27. Profile of the MET entries including GSR lines, 2014.

Detection of Rice Viruses in Infected Plants and Viruliferous Insect by Loop Mediated Isothermal

Amplification (LAMP) and its Application for Virus Disease Management in the Philippines Rice Cropping System

Smart Farming-Based Nutrient and Water Management for Rice and Corn Production

Alternative wetting and drying or AWD (irrigating the field when the water depth reaches 15 cm below the soil surface); Mid-season drainage or MSD (withdrawal of flood water 7 to 10 days before plume onset); and Saturated condition or SSC (the field is irrigated at a water depth of 2 to 3 cm when there is no visible surface water). The same trend was also observed in water productivity with respect to irrigation plus rainfall (Table 37). CF- constant flooding; AWD - alternating wetting and drying; SSC saturated soil culture; and MSD mid-season drainage.

CF- continuous flood; AWD- alternative wetting and drying; SSC- saturated soil culture; and mid-season draining of MSD.

Table 35. Frequency of irrigation, % savings and yield, 2014
Table 35. Frequency of irrigation, % savings and yield, 2014

Genetic Improvement for Upland Rice Through Marker- Assisted Selection (MAS) for Tolerance to Phosphorus

Deficiency

Philippine Rices as Substrates in the Production and Utilization of Biopigments from Monascus Purpureus Went

The optical densities of the pigments produced by the other isolates ranged from 46.52 to 343.55 AU/g dry weight (Figure 35). Optical density (AU/g dry weight) of pigments produced by the 10 Monascus isolates grown on PD broth. Optical density (AU/g dry weight) of pigments produced by the 10 Monascus isolates grown on NSIC Rc160 rice.

Optical density (AU/g dry weight) of pigments produced by isolate B2 grown on rice with different moisture contents.

Figure 35. Optical density (AU/g dry wt.) of pigments produced by the 10  Monascus isolates cultivated on PD broth.
Figure 35. Optical density (AU/g dry wt.) of pigments produced by the 10 Monascus isolates cultivated on PD broth.

XVIII. Elucidation of Growth Promotion Mechanisms of Radiation-Modified Carrageenan and Chitosan on Rice

Effect of radiation-modified carrageenan application on the growth, nutrient, and yield of rice

Foliar application of varying amounts of carrageenan was applied at rotation (15DAT), panicle initiation (40 DAT) and at the milking stage (75DAT). Treatment with 50 ppm and half NPK produced more tillers compared to the other treatments with half NPK rate. Grain yield (14% MC) of NSIC Rc216 as affected by application of two N rates and varying amounts of carrageenan (DS2014).

Plants with full NPK fertilizer gave a higher yield compared to those applied with half of the recommended NPK fertilizer.

Table 41. Tiller number per hill of NSIC Rc216 as affected by different  treatments grown under greenhouse condition (DS2014).
Table 41. Tiller number per hill of NSIC Rc216 as affected by different treatments grown under greenhouse condition (DS2014).

Effect of radiation-modified carrageenan and chitosan application on physiological processes of rice plant

  • Screenhouse and Field Evaluation of Wide Cross- Derived Rice Breeding Lines for Drought Tolerance
  • Improving Productivity and Livelihood of Wetlands and Flood-Prone Rice-based Communities in Region III
  • Validation of the Regional Rice Fertilization Guide Maps via Moet Kit and MOET-Fertilizer Requirement Calculator
  • Evaluation of Hybrid Rice-Based Diversified and Intensified Cropping Systems
  • Pilot Testing of the Fermentation and Distillation Plant for Hydrous Ethanol Production from Nipa Sap and Other
  • Accelerating the Development and Dissemination of Associated Technologies on Rice Production that are

Fifty percent (18 out of 36) of the beneficiary farmers from the pilot cluster in Barangays Bulusan, Calizon and Sta. Soil samples based on the ten color codes in the rice fertilization maps were collected and subjected to MOET tests to determine the nutrient supply capacity of individual soils. The results of a validation experiment in the municipalities of Zambales showed promising results for the application of MOET as a fertilizer recommendation generator, as yield results were better than other fertilizer recommendation guides (Table 51).

The results of the target yield determination accuracy showed higher reliability and better proximity according to the MOET application (Table 52).

Table 44. Summary data for shoot dry weight (SDW) and root dry weight  (RDW) using carrageenan and chitosan treatments in well-watered condition  (DS2014).
Table 44. Summary data for shoot dry weight (SDW) and root dry weight (RDW) using carrageenan and chitosan treatments in well-watered condition (DS2014).

Weed control practices (any practice, not just herbicides) will have a slightly different effect on individual species in a population mix. In the wet season, the average grain yields for irrigation techniques were from 3.66 to 3.98 t/ha, and in the dry season from 6.61 to 7.25 t/ha. In the wet season, grain yields ranged from 3.87 to 4.10 t/ha, and the maximum grain yield was achieved with post-emergence herbicide application at 15 DAS.

In the wet season, the weed population was considerably less than in the dry season, but more grasses were noted, such as Echinochloa colona and Fimbristylis miliacea.

Table 54. Number of techno-demonstration farm (TDF) established in 2014.
Table 54. Number of techno-demonstration farm (TDF) established in 2014.

Evaluation of agronomic and energy efficiencies of mechanical direct-seeded rice crop establishment in combination with

  • Application of Nuclear Analytical Technique for Efficient Nutrient and Water Management in Rice

General energy ratio of different tillage, sowing method and variety during dry (DS) and wet (WS) seasons, 2014 at PhilRice-CES. Manufactured and installed the parts of the 9-row zero till seed drill, such as the main frame, the seed tank with cover, the fertilizer tank with cover, the furrow opener, the seed metering plate with housing, axle and bushing, the fertilizer tank adjustment mechanism, the seed tank mounting brackets, tow bar mounting, fertilizer dosing trough, depth guide with axle and connector mounting and ground wheel (Figure 60b); The results showed that increasing the speed of the conveyor belt led to a decrease in the distance between the rice fields.

The performance of the prototype will be evaluated on the farm. a) 3D view of the 9-row zero till planting machine and (b) fabricated prototype.

Figure 59. Over-all energy ratio of the different tillage, seeding method and  variety during dry (DS) and wet (WS) seasons, 2014 at PhilRice-CES.
Figure 59. Over-all energy ratio of the different tillage, seeding method and variety during dry (DS) and wet (WS) seasons, 2014 at PhilRice-CES.

Assessing water-use efficiency of rice in different water- saving technologies using stable isotope techniques

A significantly high water productivity with respect to irrigation input was obtained in AWD (1.8 g grain per kg water) compared to CF and SSC (1.2 g grain/kg water). The same trend was also observed in water productivity with respect to irrigation plus rainfall; Irrigation frequency, water use, grain yield and water productivity of rice under different irrigation techniques, 2014.

CF- constantly flooded; AWD- alternating wet and dry; SSC-saturated soil culture; and MSD- mid-season drainage; WP -Water productivity.

Table 55. Irrigation frequency, water use, grain yield and water productivity  of rice under different irrigation techniques, 2014
Table 55. Irrigation frequency, water use, grain yield and water productivity of rice under different irrigation techniques, 2014

XXVII. Use of Stable Isotope Technique in

Improving the Soil Tests Calibration and Fertilizer Recommendation for Rice

Rice grain yield (t/ha), agronomic nitrogen use efficiency (ANUE) in soils with different fertility levels and varying amount of N applied in Region 3, 2014DS. Application of high N fertilizer to low N soil in the wet season did not significantly increase grain yield. Optimum grain yield could also be achieved even without application of N fertilizer due to low utilization of applied N in the wet season.

Rice grain yield (t/ha), agronomic nitrogen use efficiency (ANUE) in soils with different fertility levels and different rates of applied N in Region 3, 2014 WS.

Table 56. Grain yield of rice (t/ha), agronomic nitrogen use efficiency (ANUE)  in soils with different fertility levels and varying rate of N applied in Region 3,    2014DS.
Table 56. Grain yield of rice (t/ha), agronomic nitrogen use efficiency (ANUE) in soils with different fertility levels and varying rate of N applied in Region 3, 2014DS.

XXVIII. Greenhouse Gas Mitigation Potentials of Water Saving Technologies for Rice Fields in Central

Genetic Diversity of RTBV and RTSV Isolates from Different Rice Tungro-infected Areas in the

The study aims to determine the genetic variability of RTBV and RTSV isolates from different rice tungro-infected areas in the Philippines through the use of molecular markers and response screening of a differential set of rice varieties. Differences in patterns of nucleotide diversity in RTBV across geographic regions may imply that factors influencing nucleotide diversity may also vary within provinces. Differences in nucleotide diversity patterns between RTBV and RTSV across geographic regions may imply that factors affecting nucleotide diversity are affecting the two viruses independently.

The amount of heterogeneity among isolates from the same province and the degree of genetic differentiation among provinces were reflected in the phylogenetic trees.

Figure 62. Geo-tagged of provinces where tungro-infected plants were  collected. These areas included sites of outbreak reported in the past.
Figure 62. Geo-tagged of provinces where tungro-infected plants were collected. These areas included sites of outbreak reported in the past.

Pre-MET (Multi-environment Test) Evaluation of Elite Irrigated Rice Breeding Lines under the

Monitoring for Clarification of Near Canopy Environment & Hiss of Rice Under Various Types of

Based on agronomic data collected, yield and number of spikelets per panicle decreased by more than 67% in all genotypes. Sterility also increased in all genotypes, but IR64 had the highest sterility increase of up to 81.7%. Temperature and relative humidity under field conditions at Philrice, Nueva Ecija from February 19 to April 22, 2015.

Temperature and relative humidity under field conditions in PhilRice, Nueva Ecija from April 14 to May 28, 2014.

Figure 67.  Temperature and relative humidity under field conditions at  Philrice, Nueva Ecija from February 19 to April 22, 2015.
Figure 67. Temperature and relative humidity under field conditions at Philrice, Nueva Ecija from February 19 to April 22, 2015.

XXXII. Korea Project on International Agriculture

  • Variety Development and Improvement
  • Adaptability Test and Yield Performance of Korean Cultivars NL Manigbas, LB Madrid
  • Varietal Adaptation of Philippine and Korean-Bred Rice Cultivars to Organic Farming System
  • Field Monitoring of Kopia Rice Varieties to Major Insect Pests and Diseases
  • Grain Quality Evaluation of Korean Rice Cultivars Adaptable under Philippine Conditions

This test is considered very important for the future introduction of the Korean cultivar in the Philippines. But thanks to the help of Fit Corea Philippines Incorporated, farmers can now sell their palay at Php 1 to 2 above the current market price. Korean rice cultivars are known for their good eating quality and therefore command a premium price in the market.

Most Korean rice cultivars have soft and sticky cooked grains due to their low amylose content.

Improvement in Seeding and Seedling Emergence in Direct Wet Seeded Rice System

Development of Tropical Japonica Rice for Direct Seeding NL Manigbas, LB Madrid

Yield and other agronomic characteristics of the test entries in the Observational Yield Trial, PhilRice CES, 2014DS. Yield and other agronomic characteristics of the test entries in the Observational Yield Trial (OYT), PhilRice CES, 2014WS. Yield and other agronomic characteristics of the test entries in the Pre-NCT trial, PhilRice-CES Maligaya, 2014WS.

Yield and other agronomic characteristics of the test plots in the preliminary yield trial (PYT), PhilRice-CES, 2014WS.

Table 63.  Breeding materials planted and selected during 2014 dry season,  advanced and selected during 2014 wet season.
Table 63. Breeding materials planted and selected during 2014 dry season, advanced and selected during 2014 wet season.

Gambar

Table 3. Nutrient composition of the two basal media utilized in the study.
Table 5. Percentage decontamination of anther explants sterilized using  different sterilants after 15 weeks of incubation.
Figure 4. Explants cultured on the media without and with activated  charcoal and modified Euwen’s media with swollen anther (right).
Figure 6. Polymorphism observed in at least  46 nipa accessions using  molecular markers: A)EU746382, B)mPDC1R015, and C) RM14380.
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