Hy, tinh Thdi Nguydn, T^p chi Y hpc Thi/c hdnh. s6 3 (709), Tr 56
2. Mai T h i Hu'ng (2010), T/jyc tr^ng vd nh$n thirc, thdi do. thuc hdnh cua ngu&i ddn vi vi^c thu gom xt/ ly rdc t^i hai phuang thudc thdnh phi Thdi Binh ndm 2010, Luan van thae sy Y hpc, Trudng Dai hoe Y Thdi Binh
3. Ld Thi Song Huang, Ddng Trung Ki&n (2006),
"Danh gid hs^u qua dy dn eai thi^n cung d p nude sach vd v§ sinh mdi trudng ndng thdn tgi huy^n An Duang Hai Phdng", T^p chi Y hpc dij'phdng. t^pXVI, s i 2(82), tr. 50.
4. TrIn Thi KhuySn (2004), Ddnh gid mdt s6 chi tidu v4 chit Iup'ng nu&c sinh ho^t vd yiu td liin quan din cliit iup-ng nu&c ti^i 3 xS thudc huyin Kiin Xuxrng. tinh Thdi Binh, LuSn van thae sy Y hpc, Trudng Dai hpc Y Thai Binh
5. Pham Trung Kidn, Nguyin Gia Khdnh (2010), Hidu qua chuxmg trinh cung cip nu&c sach vd sinh mdi tru&ng doi v&i sue khde binh t$t tri em du&i 5 tuoi tai xa Hoang Tdy. Kim Bdng. Hd Nam: T^p chi Y hpc Thue hanh, s6 (2), Tr 41
6. Ngd Thi Nhu (2008), Nghi&n ciru mdt s6 yiu chit luxyng nu&c sinh ho^t vd binh liin quan & 6 xd
ndng thdn Ddng Hwng Thdi Binh. Hi$u qui biin phdp can thi$p, Luan an t i l n sy y hpc, Hpc Vi^n Quan Y,
7. T r I n D i e Phu va CS (2010), Nghi§n ciru mdi li&n quan gida vi sinh mdi tru&ng. nguon nu<fc hO gia dinh vd hdnh vi vi sinh chSm sde tri cua bd me vdi tinh tr^ng dinh du&ng cua tri dw&i 5 tu6i tai Vtit Nam. Bdo eao k i t qui d l tdi d p B |
8. Dgng Th| VSn Quy (2010), Ddnh gid diiu ki^n vi sinh vd mdt s6 binh t$t & hpc sinh t^i cdc tm&ng THCS tinh Thdi Binh. Luan van thge s j Y hpc, Trudng Dgi hpc Y Thdi Binh.
9. Nguyin Thj H i n g Thuy (2006), Ddnh gid kiin thue, thdi dd cua ngu&i trw&ng thdnh vd Ihi/c trang cdng trinh v$ sinh gia dinh t$i 2 huydn miin Tmng. - Lugn van thae sy Y hpc, Trudng Dgi hpc Y Thai Blnti,
10. Vl v a n Thuylt (2009), Nghi&n ciru nh$n thOe, thdi dd, thtje hdnh eua ngw&i ddn vi quin ly su dung phdn ngu&i tai Bd Thw&c, tinh Thanh Hda. Lu^n vln thae sy Y hqe, Trudng Dai hoc Y Thdi Binh
11. Nguyin v a n T i l n (2009), Nh$n thirc. thdi 6^
ihije hdnh eua ngw&i trw&ng thdnh vi b^nh sdn Id gan nhd tai 4 xa huydn Xudn Tru&ng- Nam Dir)h, Lu^n van thae sy Y hoc, Trudng Dgi hpc Y Thai Binh,
BAO CAO MOT TRlTONG HOP GIAM DINH HAI COT DUA TREN PHUONG PHAP NHAN DANG NHAN CHUNG HOC PHAP Y VA PHAN TJCH ADN TY THE
TOM T A T
Muc tieu Ap dung phuang phdp nhdn dang nhdn chung hoc phap y vd phdn tich ADN ty the trong gidm dinh hdi cot Doi twong vd phwang phap nghien cipu Bdo cdo mot tru&ng hp'p gidm dinh hai cot tai Vien Phdp y Quoc gia Ket qua vd bdn luan Gidm dmh hai cot bdng phuxyng phdp nhan dang nhdn chung hoc phdp y da xdc dinh duxyc mot sd diu hidu quan trpng nhw tuoi, gi&i tinh. lam vde, dSc diem ton thuang vd benh ly cua xuxrng Kit qua phdn tich ADN ty the da xdc dmh moi quan hd huyit thing theo ddng me giCra bd hdi cot vd ngu&i thdn Ciing v&i nhung thong tin thu thap du&c tru&c vd sau khi chit, kit hpp phan tich cdc dw kidn thu duuc qua gidm dmh dd xdc dinh duxyc chinh xdc hdi cot Ket luan Chung tdi dd gidm dmh thdnh cdng 01 tru&ng hop hdi cot bdng phuxyng phdp nhdn dang kinh diin nhw nhdn chiing hpc phdp y vd phuxyng phdp hidn dai nhw phdn tich ADN ty the
TCr khda Gidm dinh hdi cit, nhdn ehung hpc phap y, ADN ty thi
SUMMARY
CASE REPORT HUMAN REMAINS IDENTIFICATION BASED ON FORENSIC ANTHROPOLOGICAL METHOD AND MITOCHONDRIAL DNA ANALYSIS
NGUYEN DL/C NHU, NGO HUONG DUNG Vien Phdp y Quoc gia
Objectives Application of forensic anthropologicat method and mitochondnal DNA (mtONA) analysis in human remains identification.
Subjects and methods A case report of human remains identification at the National Institute of forensic medicine. Results. Human remains identification based on forensic anthropological method, we had determined some important components as age, sex, stature, trauma and pathological lesions of bone mtDNA analysis result showed that mtDNA haplotype found in bone sample matches with suspected relative's blood sample. We combined the ante-mortem and post-morten) information with data of forensic anthropology, mIDNA result to identify human remains accurately.
Conclusion V/e are successful in identification of a skeleton remains accurately, scientifically based on combination of classical identification methods as forensic anthropology and modem methods as mitochondrial DNA analysis
Keywords: Human remains identification, forensic anthropology. mtDNA.
DAT V A N D t
Giam dinh hdi cot la mot trong nhUng ITnh virc khd, r l t quan trpng trong chuyen ngdnh phap y, flap
Y HOC THU'C HANH (860) - SO 3/2013
ipng nhu c l u ngdy cang nhilu d Vi^t Nam hi^n nay.
Giam djnh hdi cot b l n g phuang phap nh$n dgng nhan ehCing hpc phap y ddi hdi phai thu thdp thdng tin trude va sau chit, thilt \^p dugc h i sa sinh hpc dya tren phan tich hinh thai xuang d l xde djnh nhu'ng y l u to ea ban nhu tuli, gidi tinh, chiing tde vd t i m vde, d i n g thdi ed t h i xdc djnh dupe dae d i l m t i n thuang hoSe b$nh ly eiia xuang [3]. Hi6n nay, phuang phdp phan tieh ADN ty t h i cho phdp xae djnh dupe m i l quan he huylt thing theo ddng me, dup-c Crng dung rdng rai trong vide xac ^ n h danh tinh hai c i t , dae bidt d l i vdi nhung m l u xuang lau nam [3, 5], Tuy nhien, mdi phuang phap deu cd nhung gidi han nhlt djnh ciia nd khi dua ra k i t luan. Vi vSy, viec k i t hpp nhieu phuang phap nhu nhan dang nhan chiing hpc phap y va phdn tich ADN ty the la rlt c i n thiet trong giam dinh hai cit, lam tdng kha nang k i t luan ehinh xac Trong nhirng nam qua, Vi0n Phap y Quoe gia dd gidm dinh hai cot eho nhilu trudng hpp blng phuang phap nhdn ehung hpc phap y hoac phan tieh ADN ty t h i giup cho cae ea quan t l tung giai quylt duae nhung vu an hoac ca nhdn, t l chLce tim 6irac hai cot ngudi thdn ciia minh. Do dd, nhan mdt trudng hpp gidm dmh hai p i t chiing tdi xin bdd cad,
Hd so-giam djnh:
Ba M Nguyen, Viet kieu d Uc, ed ngudi eha tdn la N, D. sinh nam 1934 tai Quang Binh, mIt nam 1977 tai banh vi^n Lao Cai, Hodng Lien San (eu) Mp phan CLia dng N. D. dupe xac dmh da ehon tai NghTa trang km 6, xa D i n g TuyIn, thanh p h i Lao Cai. Tuy nhien, do thdi gian 6a \du. gia dinh ba M. Nguyen bi thIt lac md, chua xdc djnh dupe phIn md ciia dng D. Gia dinh ba M Nguyen da lien lac vdi ca quan chire ndng lien quan va xde dinh dupe ba ngdi mp ehua rd danh tinh tai NghTa trang xa Dong TuyIn. Ngay 09 thang 8 nam 2012, ba M Nguyen ed dan nay d l nghi Vien Phdp y Quoc giam dmh hai cot tai ba ngdi md neu tren d l xdc dinh tim hai cot cua dng N D khdng Ngay 13 thdng 8 nam 2012, chung tdi tien hanh khai quat 03 ngdi mp d l giam dinh
Qua trinh giam djnh:
1 Thu thap thdng tin trudc chit' Theo thdng tin gia dinh cung d p , dng N D. bj ngd tir eao dap dau xuong nen eirng. sau dd 6w<?c dua di benh vien d p eifu nhung da tu vong. 6ng N D. trudc khi ehet cd 02 rang bi sau, trong dd 01 rang d5 duae d i l u tn.
2. Nghien edu ho sa b$nh an. Theo bien ban so 11/HS/PQ ngay 12/12/1977, dng N. D. chit ngay 21 thang 12 nam 1977 tai benh vidn Lao Cai, Hoang Lien San, Nguyen nhan chit: Ty mau dudi mdng cung (TMDMC).
3 Khai quat' L l n luat khai quat 03 ngdi md duae danh so la LC01, LC02 va LC03. Ngdi md thir nhlt (LC01) khdng phat hien thay xuang, ngdi mp thir hai {LC02) xde djnh la xuang tre em. Ngdi md thir ba (LC03) thu duae hai c i t nhu sau,
+ 01 hdp so khdng edn nguyen ven, cd mdt dudng nirt xuang vung thdi duang dmh phai ddi 05
cm, Xuang u ehim vd mdm xuang chum rd, kich thudc Idn.
+ Rang : Phdt hien thIy tong s i 25 rang, g I m cd 17 rang cira, 8 rang ham, mat nhai dd mdn Trong dd' cd rang so 4,7 sdu, vd than rang, edn ehdn rang nIm trong I chan rang va 01 rang hdm nhd bi sdu, da khoan tiiy
+ Cdc xuang ehi trdn vd chi dudi gom, xuang ednh tay trai vd phai; hai xuang d n g tay trai vd phai, xuang dui trai vd phai; xuang chdy trai va phai, xuang mac trai Cac xuang 6d phdn hiiy, myc nat nhieu.
+ Mdt Sd m l u xuang nhd khdng rd hinh va vi tri giai phiu
Hinh dnh dw&ng va xuang cu vung thdi duang phai 4. Phan tieh ADN ty t h i - _
Thu mau giam dmh mau xuang gom 03 chile rang cdn chan rang, M l u doi chieu la m l u mau cua dng Vd VSn K , ngudi chdu rupt (con trai ciia em gai o n g N D )
Phuang phap phan tieh ADN ty t h i [2]- - Tach chllt ADN ty the ICr m l u xuang (rang) nghi la hai edt eiia dng N. D. (ky hieu PY Q20) va m l u mau ciia dng V6 Van K {ky hieu PY.K20)
- Khuech dai ADN ty the va giai trinh tu nucleotide he gen ty t h i ciia ngudi Doi chieu vdi trinh t u thu duae vdi trinh tu chuin tren Ngdn hdng Gen quoe te (Anderson, 2005) va so sanh cae trinh tu thu duae vdi nhau [1],
STT
1 2 3 4 5 6 7 8 9 10 11
Vl tri Nu tr&n h^
qen ty the 16129 16140 16172 16183 16184 16194 16223 16234 16274 16290 16319
Trinh t u Anderson (K^RS)
G T T A A T C C G C G
M l u mau (PY K20)
A T C C 0
c
T
c
A T A
M l u rang (PY Q20)
A T C C
c
0 T
c
A T A K i t qua: Trinh t u nucleotide thu duae tir m l u rang trung vdi trinh tu nucleotide thu duae tir m l u mau.
Y HQC THirC HANH (860) - SO 3/2013
BAN LUAN
K i t qua sau khi khai quat ngdi md s6 3 da phat hifen dupe 01 b | hai c i t bi phan hiiy nhilu Tuy nhign, can cii' vdo ede m i e g i l i p h i u xuang edn Igi va cac tidu ehl v l nhan ehung hpc phdp y trfin xuang y ehIm, mdm xuang chOm, tinh trgng ya 6d mdn eua rang, t i n thuang xuong sp, chiing tdi k i t lu$n dupe.
Day la bd hai d t eiia ngudi nam gidi; khoang 40 d i n 45 t u l i ; t i m vde trung binh; phat hidn 01 dudng vd xuang cu vung thdi duang dinh p h l i ; 02 rdng hdm bj sau
K i t qua phdn tich ADN ty t h i cho thIy, Trinh ty nucleotide thu dupe tir m l u hdi c i t thu dup'c triing vdi trinh ty nucleotide tii' m l u mdu eiia dng Vd vSn K K i t luSn: Ong Vd v a n K ed lidn quan huylt thing theo ddng mp vdi ngudi cd m l u rang thu dupe tai ngdi md sd 3
N l u ehl dan thuIn k i t qu^ gidm i^nh nhdn ehijng hpe hoae ADN ty t h i trdn day chiing tdi chua thi khing djnh ehle c h i n dup'c day Id bd hai e l t cua flng N D Bdi vl, k i t q u i gidm djnh nhSn chiing hpe ptiap y trdn b | hai e l t thu th$p 6w&c da bj phan hiiy kha nhilu, chi edn mdt s6 tidu c h u i n chung tdi ddnh gia dupe la d | t u l i , gidi tinh, t i m vde, b^nh 1^' rang va t i n thuang xuang. K i t qua phan tich ADN ty t h i xac dinh dupe ngudi ed m l u xuang ed lidn quan huylt thing theo ddng mp vdi dng Vd v a n K chir chua thi khIng djnh c h i c c h i n m l u xuang dd Id eua dng N.D Theo qui luat di truyin ADN ty t h i thi nhOng ngudi c6 cung ddng mp sd ed eiing trinh t y ADN ty t h i [4]
Do v$y, trude khi dua ra k i t ludn eIn phai kit hpp, d l i chilu, sc sdnh t i t ca cac dO" ki$n chinh thu thdp dupe qua qud trinh giam dinh:
Du' ki^n chinh Gidi tinh Tu6i khi mIt
Tim v6c Rang
Ton thuang Ket qua phSn tich ADN ty
the
Thftng tin triidc chfet do gia dinh cung d p
Nam 43 Trung binh 02 rang sau (01 rang ham 3&
difeu In}
Nga cao dgp dau xuong nen cung Trinh ti/ nucleotide thu dug
Hi sa b$nh an do b$nh vi$n cung d p
Nam 43
Nga cao dap diu, chan doan TMDMC
K I I qua giam djnh nhan chiing hpc phap y
Nam 40-45 Trung binh 02 rang ham sau {01 rang
4 7 da khoan tuy) Du'dng v& cu xuang thai
du-ong phai ti> mlu rang trung vdi trinh ty nucleotide thu duae tir mau mSu
cua 6ng Ve Van K Dua vao nhirng du- kidn quan treng, can thiet
phue vu cho vide nhan dang hai e l t neu tren thdng qua thu thap thdng tin va k i t qua phan tich xuang, k i t qua phan tieh ADN ty the, chung tdi dua ra k i t iudn giam dinh Bd hai c6t dupe khai qudt tai ngdi md s i LC03 la hai cot dng N D.
K £ T LUAN
Mdi phuang phap nhan dang hai e l t d i u cd nhirng gid tn va nhung han e h l nhat dmh, dde biet d l i vdi nhung bd hai cot lau nam bj phan hiiy nhilu De ed dupe k i t luan giam dmh hdi cot chinh xde, khoa hpc ddi hoi ket hap phuang phap nhdn dang kinh diln nhu nhan chung hpe phap y va phuang phap hien dai nhu phan tich ADN ty the. Trong dd, viec thu thap thdng tin trudc va sau c h i t Id mdt trong nhirng nguyen t i c va la yeu t l rat can thilt de tang them kha ndng k i t luan giam dmh chinh xac, Dua tren nhirng phuang phap tren, chung tdi da giam djnh thdnh cdng 01 trudng hop xac dmh hdi eot mdt each ehinh xae, khach quan, khoa hoe
TAI LIEU THAM KHAO
1 Anderson S , et al Sequence and organization of the human mitochondrial genome, Nature (1981) 290 4 5 7 ^ 6 5
2 Angle Carracedo (2005). Forensic DNA Typing Protocols (Methods in Molecular and Biology) [Hardcover], Methods in Molecular and Biology Vol 297 Humana Press Inc.
3, Braday J. Adams Forensic Anthropology, Inside Forensic Seienee. Chelsea House Pulishers (2007). USA
4, Giles R. E,, Blanc H , Cann H M,, and Wallace D. C. Maternal inheritance of human mitochondnal DNA, Proceedings of the National Academy of Sciences (1980) 77:6715-6719
5, ICCR. Mising people, DNA analysis and identification of human remains A guide to best practice in armed conflicts and other situations of armed violence. Second edition. (2009)
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