Ket qua nghifin c u u khoa hpc B V T V - S 6 5/2016
7 ngdy phun; vd Anvado 100WP cd hifiu Ivc cao nhdl dat 79.76% sau 10 ngay phun (bang 1).
4. K£T LUAN
- Rfip mufii ngd ( R maidis) cd xu hudng phdt sinh vd gdy hgi nfing trfin gidng cao luang ngpt KSC105 khi trdng vdi mfit dfi gieo trdng 10,3 vgn vd 15.4 vgn cdy/ha so vdi cdng thuc mfit dfi trdng 7,7 vgn cdy/ha.
- Rfip mufii ngfi eung cd xu h u d n g phdt sinh, gdy hgi nfing han trfin giong cao luang ngpt KCS105 d u p c bdn vdi hdm l u v n g phdn dgm cao 0" lidu lupng 250N + 8OP2O5 + II2K2O;
300N + 96P2OS + 134K20 vd 350N + II2P2OS + 156K20/ha, m$t dfi rfip mufii ngfi cao han so mfit dfi rfip mufii ngd d lidu lupng phdn bdn 200N + 64P205 + gOKjO/ha.
- Thudc trir sfiu Elsin 10EC (300 ml thude/ha).
Chess 50WG (300 g thudc/ha) vd Oshin 20WP (200 g thudc/ha) cd hifiu Ivc phdng trir lodi rfip mufii ngd trfin gidng cao luang ngpt KCS105 trdng trong vy Xudn 2014 tgi Thdi Nguyfin. Hifiu Ive phdng tru eua cd 3 logi thudc ddu dgt trfin 80% d thdi didm 3 ngdy sau phun, tdng ddn vd duy tri d mirc trfin 90% d thdi diem 10 ngdy sau phun.
Ldi cdm an
Cdng trinh nghifin cdu dupc \h\fc hifin bdng kinh phi cua dd tdi "Nghifin o / u bifin phdp phdng trir rfip mufii vd bfinh thdi rd trfin cao luong ngpt cao sdn
nhfip nfii t u Nhfit Bdn"; md sd B2014-TN02-03;
Chu nhifim dd tdi; TS. Duong Thj Nguyfin, Tnrdng Dgi hpc Ndng Ldm - Dgi hpc Thdi Nguyfin.
T A I L I $ U T H A M K H A O 1. Blackman R.L. and Eastop V.F., 2000. Aphid on the word's Cmps, An Identificalion Guide. 2nd edn, Vidley - Interscience.
2. Dik A.J. and van Pelt A.J.. 1992. Interaction tietween phyllosphere yeasts, aphid honeydew and fungicide effectiveness in wheat under field conditions'. Plant Pathc^ogy, 41, 661 - 675.
3. Duang Thi Nguyfin. Hodng Bich Thdo, Nguyfin Thi Mfio, NguySn Th| Phuang Oanh. Gidp Thj Thanh.
Mai Vdn Qudn, Trjnh Xudn Hogt, 2015. Xdc djnh nguyfin nhdn gdy bfinh thdi rS cao luang ngijt {Sorghum bicolor L) tgi Thdi Nguyfin. T$p chl Khoa hoc Ndng nghi$p Vidt Nam 6 {52), 121 -128.
4. Qudch Thj Ngp, 2000. Nghifin cdu rfip mu^i.
(Homoptera: Aphididae) tren mfit sd cfiy trdng chinh 6 ddng bdng song Hdng vd bi§n phdp phing trir. Lu$n dn tiin sT ndng nghidp, Vi$n Khoa hpc Ndng nghidp Vi$tNam.
5. Studebaker G.E.. Lorenz G. and Akin S.. 2013).
Grain sorghum insect control. University of Arkansas, Division of Agriculture, httpV/www.uaex.edu.
Phdn bl^n: GS.TS. NCVCC. Phgm Vdn L i m
si NGHieN ClfU A N H mrCrUG C U A C H ^ P H A M S I N H H Q C Pseudomonas putida O^N SINH TRirerNG VA TY L? S 6 N G CUA CAY H 6 TlfeU G I A M HOM
TAI PLEIKU, GIA LAI
Effects of Pseudomonas putida Biological Product on Growth and Alive Ratio of Stem Cutting Blacit Pepper in Pleiku, Gla Lai
NguySn T h | Hu$, Nguydn Quang C a , L i Vdn Chdnh v d Trdn T h | T h u Hd Tnjt^g Dal hoc Ndng Ldm. Dg/ hQC Hui
Ngdy nhin bdi: 15.07.2016 Ngdy chip nh$n: 03.08.2016 Abstract
Pseudomonas putkia bnlogicai product had effects on growth of black pepper stem cutting. The number of leaves were 24.92 and 16.94 in treatments with and without P. putida. respectively. In the treatments vnth P.
putida. the mam stem height was ranging from 17.99 - 44.36 cm, 8-ignifk:anttly higher than that in the control*.
12
Kdt qua nghifin c u u khoa hpc BVTV-So 5/2016
Besides, the number of roots/cutting, average of root length and longest root were 244.21. 4.85 and 7.98, respectively, a i d significanlJy differences with the control (the values were 211.50. 2.85 and 6.01 respectively). At ttie 90 days after cutting, in treatment with P. putida, alive ratio reached 98.08%, higher ttian the control.
Keywords: Pseudomonas putida bk)togical product, number of teavesfctem cutting, number of root/stem cuttng. numtier of dead stem cuttkig, percentage of shoot, the height of main shooL
I . D A T V A N B £
Cdy hd tifiu {Piper nigrum L.) Id cdy cdng nghifip cd gid t i kinh td ldn d Vifit Nam vd Id cdy gia v| cd tinh chdt thuang mgi quan trpng nhat, hifin dang cd gid tii xudt khdu cao. Hdng ndm, hd tifiu Vifit Nam chidm ti trpng 30 - 50% trong tdng gid trj lupng gia vj mua bdn trfin the gidi.
Cdy hd tifiu ed the nhfin gidng bdng hgt vd nhdn gidng vd tlnh bdng cdc logi cdnh. Ky Uiufit nhdn gidng bdng hgt thudng dp dgng v d i mgc dfch nghifin euu thf nghifim, lai tgo gidng vd hau nhu khfing thvc hifin trong thuc td san xudt vi cdy eon khdng dam bao dfic Unh di truydn cua
^ y mp. cdy ydu vd chfim phdt tndn {Tdn Nu' Tudn Nam, 2005). Cdy con gieo tir hgt thudng cd ty Ifi bat thudng cao, cdy con phai mdt 7 ndm sau mdi cho Irdi vd mfit sd cfiy cd thd cho hoa dan phdi (Trdn Vdn Hda vd cfing s v , 2001).
Gidm cdnh Id phuang phdp dd thvc hifin, phd bidn nhdt d u p c dp dgng cho hdu hdt ede nudc trdng tifiu ^fin thd gidi Cd thd s u dgng ednh thdn, cdnh l u a n vd cdnh qud dd nhfin gidng hd tifiu. Ldy hom t u thdn chinh dd san xuat cdy gidng rdt phd bidn hifin nay d Tdy Nguyfin. Hom thfin cd thd d u p c u a m trfin cdc ludng u a m cho ddn khi ra re rdi dem trdng, phuang phdp ndy khde vdi u o m tnjng bdu. V d i cdch Idm ndy ngudi ndng ddn cd thd logi bd t}dt mdt sd hom ydu xdu, bfi re khdng dgt yfiu cdu trudc khi dua ra trdng (Tfin N u Tudn Nam. 2005).
Chd pham sinh hpc Pseudomonas putida d u p c tgo ra ti> chung vi khudn ddi khdng Pseudomonas putida d u v c phan Ifip h> rd cdy hd tifiu khoe d Vifit Nam vd cd kha ndng tgo ra hogt ehdt putisolvin, cd kha ndng phdn giai bdo t u dfing ciia ndm bfinh chet nhanh vd Idm gidm ddng kd ty Ifi bfinh ehdt nhanh eua cdy tifiu gidm cdnh d v u d n u a m trdng trong dat nhtdm ndm Phytophthora capsit^. Ngodi ra cdc ehung P. putida cd kha ndng tgo ra indole acetic axit (lAA) Id mfit ehdt kfch thfch sinh tnfdng cho cdy trdng (Tran Thi Thu Ha, 2007). Xuat phdt tir vdn dd trfin, chOng tfii tidn hdnh nghifin c d u s u dgng d i d phdm sinh hpc P. putida dd xO- IJ" u a m hom ttidn nhdn gidng hd tifiu trfin ludng nhdm san xuat cdy gidng M\6e.
2. VAT L1$U VA PHUUNG PHAP NGHIEN CUV 2.1 Vgt lifiu nghien c i r u
- Gidng hd Ufiu; Gidng hd tifiu VTnh Linh..
- S u dgng hom thdn 1 ndm tudi
- Chd phdm sinh hpc P. putida d u p c Idm tir Chung vi khudn ddi khdng P. putida 199B (Tran et al, 2008) vfil mfit dfi 10" CFU/g chd phdm.
2.2 P h t K m g phdp nghifin c i r u
Thi nghifim d u p c thvc hifin vdo thdng 7 ndm 2015 tgi Thdn 1. xd Tdn S a n . thdnh phd Pleiku, tlnh Gia Lai. Thi nghifim bao gdm 2 cdng thirc:
Cflng thifc ed x u ly chd phdm P. putida vd cdng thirc ddi chirng (Khfing x u ly chd phdm).
Bdng 1 . Cong t h i r c t h i n g h i d m Chi tifiu theo ddi
Giong tifiu Ludng ucrm
TT lfi phdi trfin ddt vd phdn
XOr ly hom gidng
Ddi ehCrnq VTnh Ltnh - Su dgng ddy dc Mdi hom gidng ddi 5-6 ddt Lgdng uam cd kfch thudc rfinq 1,5 x 5m Ddt vd phdn dupc trfin theo ty lfi: 4 ddt +1 phdn chudng hoal mge + 0,5% super idn + 1% vfii bfit
Khdng
Xu (y Pseudomonas putida VTnh Linh - Su dgng ddy dc
Mdi hom qidnq ddi 5-6 ddt
Ludng uam ed kich thudc rfing 1,5 x 5m
Ddt vd phdn dupe trfin theo ty Ifi: 4 ddt + 1 phdn chudng hoai mgc + 0.5% super Idn + 1 % vdi bfit Chdm mifing cdt hom gidng vdo tih^ phdm P.putida. dd 5 phut vd chdm ldn 2 rdi gidm vdo ludng (SOOg chd phdm/1000 hom qidnq)
Kdt qua nghien ciru khoa hpc B V T V - S 6 5/2016
Chdm sdc hom gidng sau khi gidm theo phuang phdp cua Tdn Nir Tudn Nam (Tdn NCr Tuan Nam 2005, 2008).
Odi vdl cdng thde xir IJ chd phdm sinh hpc P.
putida, d cdc thdi diem sau 1 vd 2 thdng gidm hom tidn hdnh tudi chd pham P. putida vdi lieu lupng 1 kg hda vdi vdi 50 lit nudc tudi cho SOO hom giong vd cdng thirc ddi chirng tudi nudc Id.
Theo ddi djnh ky 2S, 50. 65 vd 90 ngdy sau khi gidm cdc chi tifiu ty Ifi bfit mdm (%}. dfing thdi ra Id (Id) vd tdngtm-OTig chidu cao thdn chlnh (cm), sd lupiig re/hom (re), chidu ddi r l (cm) (Nguyen Minh Hieu. 1998) vd ty lfi hom chdt (%) (Tdn N& Tudn Nam, 2005).
Cdc chf tifiu duvc theo ddi trfin 5 didm. 4 gde cua ludng uam vd tgi giao didm hai dudng chfio eua ludng. Mdi didm theo ddi 50 hom gidng.
Rifing vdi chi tifiu ty Ifi hom ehdt (%) thi Uieo ddi trfin ca ludng. Tgi thdi didm 25 ngdy sau gidm hom, hom tifiu duvc mang ra trdng d vudn san xudt thi mfit hom gidng duvc tlnh Id ehdt khi thdn hom bj hfio, ddu gde bf thdm den vd khdng phdt sinh re mdi.
Cdc thdi didm theo ddi sau khi mang hom gidng ra trdng d vudn sdn xuat cdy tifiu eon duvc tinh Id chdt khi todn bfi Id trfin cdy chuydn mdu vdng, njng vd ttidn cdy hfio.
Cdc sd lifiu duvc xu IJ thdng kfi T-test sii dyng phdn mdm thdng kfi chuyfin dgng SPSS 20,0.
3. KfeT QUA NGHIEN Cl>U VA T H A O L U A N 3.1 Anh hudng ciia chd phdm sinh hpc P. putida ddn tif 1$ b$t mdm vd d^ng thdi ra id
Bdng 2. Anh hu^ng ciia chd phdm sinh hpc P. put/da ddn d$ng thai ra Id cua h& tieu gidm hom
Ngiy sau giam 25 60 65 90
S6 ti mM t30 thSnh/chii (14) D6i chi>ng
0,50 1,09 6,22 16,94
Xi>ly
P. putkia0,47 1,98 8,36 24,92
T-test 0,817 0,003 0,041 0,000 Ghi c/li): Trong m « hing, T-tesI < 0,05 sai khic cdf nghta
Kit qua d bing 2 cho th4y, * thdri i3i4m sau
25 ngiy sau giam hom s4 la m*i fiio thinh c«
tang I6n nhu'ng IthSng d i n g k l vi khdng cd sv khac bi$t vk thing kS giO'a hai cfing thl>c thi nghidm. TJ 1$ bjt mkm 6 thdi a i l m n i y la 28%
va khfing ofi Skr sai khio giOa hai ofing thi>o (s4 liju khfing trlnh biy). O thfii d i l m 50 vi 65 ngSy sau giam hom, s6 t i mfii tao thanh trong hai cfing thi>c cfi sv tang I6n rfi rfit, cho th^y hom tifiu sau khi mang ra tring dfi bat d4u bfin r l , s6 la mfi'i t^o thfinh d cfing thCrc ofi sO dgng ch^
pham P. putida (8,36 lfi) cao han h i n so vfii ofing thijo d i i chi>ng (6,22 lfi).
Cf thfii dilm 90 ngfiy sau gifim hom, cfiy con di vfio phat triln i n djnh vfi thich nghi tit vfi'i dilu kifin CT vu'fi'n s i n xult, do dfi s i i i mfi'i tgo thinli 0' hai cfing thi>o ting Ifin ding k l so vfi'i lin theo dfii tru'fi'c dfi. C/ thfi'i d i l m 90 ngfiy sau khi giam hom, s i l i ting thfim Ifin d ofing thi>c cfi sO dyng P. putida la 16,56 l i , cao hon h i n so vfii d4i ChCrng l i 7,98 l i v i cfi s y k h i c bifit cfi y nghTa vi mfit thing kfi. O l l u n i y cho thiy sir dgng ch6 phim P. putida cd khS nfing kich thich ra l i fi hi tifiu giim hom. K i t q u i n i y phCi hap vfii k i t qud da nghifin ctru cua Pham Thj Anh T h i o v i 7 ' Thj Thu H i (2012) va k i t q u i nghifin oi>u L i m Minh van va T r i n Thj Thu H i (2015) khi dgng c h l phim P. putida nam hom giing h i bing day lycn vfi thin.
3.2 AnK hirfiing caa c h l p h i m sinh I P. putida ain d^ng t h i i t i n g trirfimg ch cao hom tieu
T i c dfi, dfing thii Bng trufing chilu cao cay B tifiu phin finh khi nfing hip Ihu, sij dgng chit ( dufing d l thiJc diy qua trlnh sinh tiu'fing cOa cfiy.
B i n g 3. Anh hipfirng ciia c h l p h i m sinh hf P. putida d i n d f n g t h i i t i n g tnrding
c h i l u cao c i n h ciia hom tifiu g i i n g
Ngay
sau giam 25 50 65 90
Chilu cao thin chlnh (cm)
€)ii ChCrng 6,63
6,87 14,00 34.88Xi> i; p.
putida
6.51 6,69 17.99 44.35
T-te:
049!.
040 000 0,00 Ghi chd: Trong m^t hdng. T-test < 0 05 khde c6 y nghTa
Kit qua nghifin cuu khoa hpc BVTV-S6 5/2016
Kit qui d bang 3 cho thiy, d ttifii dilm 25 v i 50 ngiy sau giim hom, scr tfing trucmg chilu cao cua hom tifiu giOa ofing thCrc cfi xir ly ohl phim P putida vfii cfing thiJc d i i chCrng gin nhu khflng dfing k l . Tuy nhifin, cr thfi'i dilm 65 ngay sau giam hom, qui trinh bfit mim cOng vfii scr tfing trvCTng chilu cao cOa chii mfii tao thinh lim chilu cao than chfnh cfia hom tfing manh.
Cfing thCrc xu' IJ c h l phim P. putkia cfi chilu cao cfiy dgt 17,99 cm, cao hon hin so vfii d i i chCrng (14,00 cm).
O thfii dilm 90 ngiy sau khi gifim hom, s i la mfi'i tgo thinh tang nhanh, tgo dilu kifin thuc diy qui trlnh quang hap tich IDy vfit chit, lim ling kha ning hlnh thanh dit than mfi'i, do dfi chilu cao than chlnh cua hom tifiu cOng ting ISn nhanh chfing. Ct cfing thCrc cfi sO dgng chl phim P.
putida, chilu cao ciy dgt 44,36 cm v i cfi scr khic bifit cfl J nghTa v l mfit thing kfi so vfii d cfing thCrc dii ChCrng (34,88 cm). Kit qui niy cOng d i duac Trin Thi Thu Hfi (2008) cfing b i P. putkia cfi khi nfing kich thich sinh truo'ng cua cay h i tifiu va ciy dira chufit do vi khuin niy cfi khi nang tilt ra chit kich thich sinh truimg indole acetic axit (lAA).
3.3 Anh hirfi'ng ciia c h l phim sinh hpc P. putida d i n k h i ning ra r l v i k h i nSng lich iOy v^t chit cua ciy hom tifiu
Bing 4. Anh hu'fi^g cua c h l phim sinh hpc P. putida d i n scr ra r l cfia hom tifiu
(25 ngiy sau glim)
Cfing thOc
D i i chOmq XCrlJ
P. putkia
T-tesI
S i rl/hom
(rl) 211,50 244,21
0,007II I
6.01 7,98 0.004
C h i l u dai trung
blnh r l (cm) 2,87 4,85 0,000 Ghi chd: Trong m^t hdng, T-test < 0,05 sai khde c6 y nghTa
Vdi cfing thdc ddi chirng, ngudi dfin thudng dd nguyfin bfi Id d hom gidng thay vl tia bd.
Chinh difiu ndy Idm chfim s v bfit mdm song l?l Idm tdng khd ndng hlnh thdnh rd ciia hom tifiu (bdo cdo nghidn cOu khoa hpc 2001 - 2005). Tuy
nhifin, ket qua thi nghifim d bdng 4 eho thdy, sau 25 ngdy sau khi gidm trfin ludng, cdc hom tifiu d cdng thuc c d s u dgng chd phdm P. putida cd sd luvng rfi/hom, chieu ddi trung binh re/hom ldn l u v t id 244,21 r l / h o m , 4,85 cm/rd, 7,98 cm/hom, cao han hdn so v d i cdng thi/c ddi chung khfing s u dgng chd phdm {211,50 re/hom, 2,87 cm/rd, 6,01 cm/hom). Ket qua ndy eho thdy che phdm P.
putida cd kha ndng kich thich s v ra re mdi d tifiu gidm hom, giup tdng kha ndng sdng cho hom tifiu khi mang ra trdng d v u d n san xudt.
Kha ndng tich IQy vfit chat cua hom tifiu Id ch!
tifiu ddnh gid khd ndng hut nudc, dinh d u d n g , quang h v p vfin chuydn cdc chdt trong edy dd tich luy vfit chdt vdo cdc bfi phfin sinh t n / d n g cdnh, Id v d r d .
Bang 5. A n h hu-drng cua c h d p h d m P. putida dfin s i n h khdi cdnh vd rd cdy
(25 ngdy sau gidm)
Cfing thuc D i i ohCrnq XCrlJ P. putida T-test
K h i i lirang c i n h (q/cinh) TuCTi 64,43 76,85 0.406
Khfi 10,43 12.13 0,524
K h i i Iu'O'ng r l (q/rl) Tu'oi 4,65 9,05 0 0 4 6
Khfl 1,48 4,98 0,016 Ghi chCi: Trang mdt cdt, T-test < 0,05 sai khic cd y nghta
Kit qui d bing 5 cho thiy, cflng thCrc cfi su dgng chl phim Pseudomonas thi khii luang tu'ffi v i khfi cua cinh lin li/at l i 76,85 g v i 12,13 g, diu cao han so vfi'i d i i chi>ng (64,43g v i 10,43g). Tuy nhifin khfing cfi su khfio bifit v l mfit thing kfi dii vfii khii li/ang tirai v i khfi cua cinh giCra 2 cing thiJc.
SO dgng chl phim Pseudomonas lim ting s i lu'ang rl/hom vfi chilu d i i r l din tfii lim tang khii lirang tual v i khfi cda r l . 0 cflng thCrc cfl su dgng chl phim Pseudomonas, khii lirang tirol v i khfl cga r l lin luot l i 9,05 g va 4,98 g, cao han hin so vfii d i i chCrng l i 4 65 g vai,48g.
Tflm Igi, cfing thuc cfi xir IJ chl phim
Pseudomonas diu cfl inh huing din dflng thii
ra la, chilu cao cfinh. s i lu'ang r l , chilu dai r l
va sinh khii tiroi v i khfi cfla cfinh v i r l , dilu nay
cfl thi giii thich do chung vi khuin P. putida
Kdt qua nghifin c i j u khoa hpc B V T V - S 6 5/2016
1993 cd khd ndng tidt ra chdt kich thich sinh trudng indole acetic acid (Tran e( al., 2008) nfin cd tde dgng kich thieh cfiy hd tifiu gidm hom sinh tnrdng phdt tridn tdt hon so vdi ddt chung.
3.4 A n h hu>dmg cua c h d p h d m s i n h hpc Pseudomonas ddn t y i f s d n g
Bang 6. A n h huv^ng cua chfi p h d m s i n h hpc Pseudomonas ddn t ^ I f s d n g
Ngay
sau giim 25 50 65 90
Tvl Dil ChCrng
60,00 91,67 81,82 88,89
> sinq (%) Xu'lJ P. putida
91,91 94,60 96,22 98,08
T-test 0,001 0,218 0,001 0,014
Ghi chO: Trong cOng mdt hing, T-test < 0,05 sai khic cd f nghTaBfinh c h i t nhanh do n i m Phythophthora capsici l i bfinh cgc kJ nggy h i l m . nfi cfi t h i gfiy bfinh trfin t i t c i cfic giai dogn cua cfiy h i tifiu v i gfiy thifit hgi kinh t l r i t Ifin, do dfl vific phflng bfinh ngay tCr giai dogn b i t d i u tiam hom g i i n g l i h l l sirc c i n thilt. O giai dogn v u f i i i u'am bfinh c h i t nhgnh t h i hifin c i c trifiu ohOng nhu: t h i i nhOn p h i n r l , l i h i tifiu cfl hifin tuang hfio xanh hofic v i n g .
Qug theo dfii h i u h i t c i c hom tifiu g i i n g c h i t d i u cfi trifiu ChCrng t h i i den d i u g i c , khfing phfit r l mfii (fl' gial dogn 25 ngay sau g i i m hom) v i d cac giai dogn theo dfii k h i c trfin c i y tifiu con trong vufl-n s i n x u l t , mflt s i c i y cfl b i l u hifin t h i n hfio, l i c h g y i n v i n g hofic hfio xanh, khi n h i Ifin qgan sfit t h i y p h i n r l cfiy bj t h i i nhOng. Qug day cfi I h l k h i n g djnh nguyfin n h i n g i y c h i t hom tifig chu y i u d giai dogn g i i m hom l i do n i m P. capsid. Tuy nhifin, qua xCr IJ vfii c h l p h i m P. putUa chfing tfii nhfin t h i y r i n g c h l p h i m khfing chl glClp t i n g k h i n i n g sinh tru&ng p h i t t r i l n cua hom h i Bfiu g i i n g m i cfin cfi t i c dgng Crc c h l scr phat I r i l n cga bfinh hgi v i t i n g ty Ifi s i n g hom tifig g i i n g trfin t i t c i c i c gigi dogn theo dfli 6' vgfl'n u a m .
Hom tifig g i i n g d u o c mang ra t r i n g d vcrfin s i n x g i t sau 25 n g i y d u a c g i i m Irfin l u i n g . Chlnh d i l u n i y i n h h u i n g d i n ty Ifi s i n g cua hom g i i n g . Cl giai dogn 25 n g i y , xO ly c h l p h i m
P. putida l i m t i n g t i lfi s i n g oija c i y g i i n g so vfii cfing IhOc d i i chirng. Ty Ifi s i n g cua hom tifiu o cflng thCrc xfl' IJ dgt 9 1 , 9 1 % cao han h i n so vfli cflng thO'c d i i chOng (60.00%). Vfi'i cfing thO'c sir dgng c h l p h i m Pseudomonas, ty Ifi c h i t cfla hom chi 3,78%. Trong khi dfl vfi'i cfing thCrc d i i ChCrng, ty Ifi hom c h i t Ifin td'i 19,18% tgi thfii d i l m 65 ngfiy sau gifim hom.
Q thfi'i d i l m 90 ngfiy sau g i i m hom, hom g i i n g d i thich nghi vfi'i d i l u kifin vu'fi'n s i n xuit thi tl Ifi hom c h i t d i u g i i m . Tuy nhifin t i Ifi sing cfla hom g i i n g d cfing t h u ^ cfi sir dgng c h i p h i m P. putkia (98,08%) v i n cao h a n h i n so vfii cfing thOc d i i chO'ng (88,89%). B i l g n i y cho t h i y , vific sfl' dgng c h l p h i m l i m tSng k h i ning t3 r l cOng nhu dfl d i i r l cug hom g i i n g tCr dfi Ifim tfing k h i n i n g s i n g cug hom g i i n g khi tring trfin vufl'n s i n x u i t .
4. K £ T LUiliN
Sgu 25 n g i y sgu gifim hom, tJ Ifi bfit m i m gi&g cfing thOo d i i chfl-ng v i cflng thO'c xu- IJ Pseudomonas putida lfi khflng cfl scr sgi khic.
Tuy nhifin.tCr sau thW d l l m 50 sau g l i m hom s i l i cfl sg' sai k h i c , d cfing thO'c cfi xfl' IJ c h l p h i m P. putida (1,98 l i ) cao h a n h i n so vfii cflng thCi'C d i i ChO'ng (1,09 lfi), v i s i l i dgt 24,92 l i khio bifit v l t h i n g kfi so vfi'i cflng thCrc d i i chOng (16,94 l i ) tgi thfli d i l m 90 n g i y sau g l i m hom.
Cfing thOc xir IJ c h l p h i m P. putkia cfi i n h hu'd'ng d i n c h i l u cao hom g i i n g v i tfing t r u i n g k h i mgnh so vfli d i i chirng (14,00 cm) sau 50 - 65 n g i y sau g i i m dgt 17,99 c m . Cfing thO'c cfl xi>
IJ c h l p h i m sinh hoc P. putkia thi cfl s i luvng r l / h o m l i 244,21 r l / h o m , r l d i i n h i t d i i l i 7,98 cm v i c h i l u d i i r l trung blnh la 4,85 cm d i u cao han so vfi'i cflng thCrc d i i chO'ng.
C h l p h i m sinh hoc ofi i n h h t f i n g d i n tJ 1$
s i n g cua hom dgt 98,08% trang khi d cflng thCrc d i i ChCrng dgt 88,89% sau g i i m 90 n g i y
T A i L i f u T H A M K H A O 1. Bdo Cio nghifin ciru khoa hoc 2001- 2005 tJghiin COu chon tao gidng vi hi thing kf thuit ling hvp nhim Phil Uin ciy hd tiiu vOng Tiy Nguyin Vifin KHKT Nfing Lfim nghifip T i y Nguyfin H J ' ^ ^ ^ ' • f * ' T « Thu Hfi v i Nguyin HOu S ^ l , S ° ^ : i ? " " d "Sng nhan hem aling h i liSu VTnh Unh bSng hom M n vfii s4 d i t W n thin wSc nhau,
