KHOA HOC CdNG NGHE
UIMG DLJIMG C H i T H ! PHAIXI TLT TROfVG CHOIM GIOIXIG L L I A IVIAMG GEIXI IVILJI T H Q M [Oryza satit/a. L)
H4 Ngoc HifoS Nguygn 114 KhAnh TrSn', Nguyen TTii LangS Biii Chi Buu' T6MTAT
Nghien cmi nay ap dung ban do GOT tu quan th^ F7 tren to hop lai OMCS2000/KhaoDawmali 105, mang gen thom thong qua ung dung chl thi phan tir de cai tien giong liia thoin. Trong quan the OMCS2000/KhaoDawinali 105 da ghi nhan co gen lan phan ly voi ty so (3:1) tren qudn the F2 va F3. Co su da hinh vol hai chi thi RM223 va RM 3459 tren nhilm sic die so 8. Ket qua nay co diroc nhcr sir dung 21 chi thi phan hi tren nhi^m sac the so 8, khoang each di truyen tii 0-56 cM. Cac ca the con lai diroc lira chpn phai mang gen d6ng hop troi tren viing nhiem sac the voi 4 dong co 100% vung gen triing hop voi ca the bci (Khao Daft- Mali 105), mang gen muc tieu miii thorn nha GOT. Cac ca the dirge chon co miii thom la: dong 19 (F2-4-6-16-1-7-19); dong 20 (F2-17-1-16-19-8-20); dong 29 (F2 -18-3-1-8-106-29); dong 31(F2-31-7-&-38-10-31).
Nang suat ghi nhan co dong 29 (F2 -18-3-1-8-106-29) cho nang suat cao nhat co y nghia so voi doi chiing. Do do, ket qua nay la nhung tai lieu tham khao quan trong cho viSc chpn tao giong liia thom.
Tii kh6a: Chi thi pban td, di truyen chon gidng, GGT, min thom.
1 . MO DAU
Hirong vi va vi ngon cua gao thom diroc ngiroi ti6u dung tr^n th^ gim rat ua chuong. Miii thom trong gao duoc kiem soat v^ mat di truyen bdi cac gen trong nhan t^ bao va khong phu thupc vao di truydn t^ bao chat, sir di truyen co the lifin quan den cac gen bien doi ma khong co tac diing cua cay me.
Di^u nay phii hop voi Hang etal. (1995) bao cao rang miii thom cua la lila duoc kiem scat boi kieu gen bao tOr (2n) trong khi a hat gao, miii thom duoc kiem soat boi noi nhu (3n), khong c6 miii thom la dac tinh chi phoi giong do anh huong ciia bd me khong ph6i hpp tot voi nhau. Mgt gen lan nam tren nhifim sac the so 8 da duoc xac dinh la gen mang tinh trang huong thorn (Bradbury et al., 2005; Shu et al, 2008;
Lorieux etal., 1996) da khang dinh moi lifin kfit chM che giira chi thi RG28 va . ^ ( 5 , 8 cM) tren nhifim sac the 8 va xac dinh duoc hai locus cho huong thom.
mpt tren nhifim sac the 4 va mot h-fin nhifim sac the 12. Mac dii vay. ngiroi ta da xac dinh duoc trong hau het cac giong Itia, miii thom dirge kiem soat boi mot gen lan duy nhat dmh vi ti-fin nhifim sac the so 8 ctia loai liia trong 0. sativa (U et al.. 2006); Lang va ctv, 2008). Trong bai bao nay, hinh bay cac ket qua phan tich di tmyfin va lap ban d6 GGT vk mui thom tir gidng KhaoDawMah 105 va cung cap chi tiet ket qua phan tich moi lifin ket voi mui thom lam co so
cho vific lua chon gidng liia lai dirpc trg giiip nho danh dau phan tir trfin nhifim sac the so 8 nham phuc vu cho chuong trinh nhan gidng trong lirong lai.
Z. VAT L i u VA PHUDNG PHAP 2.1.VStU§u
Gidng Khaodaw Mali 105 cua Thai Lan la gidng Ilia miia co miii thom; OMCS2000 tir Vifin Liia DBSCL diroc phat tnen rpng d DBSCL trong thap nien 2000-2010. Hai gidng ddi chimg Jasmine 85 (thom) tir Hoa Ky va OM1490 (khong thom) ciia Vien Liia DBSCL
2.2. Phuong phap 2.2.1. Danh gia min thom
Cac xet nghifim trfin la liia, 1,7% voi KOH da duoc ap dung theo Sood va Siddiq (1978). Phuong phap trfin hat gao cai tien (Lang va ctv, 2008): trfin hat liia duoc hoc vo trau bang tay va dugc xat trang bang may "test miller" trong 1 gid. Mudi hat cua mdi ca th^ duoc nghifin bang may Wil grinder, tdc dp trung binh. Bgt gao ctia moi hat duoc dat trong mgt hop plastic 5 x 5 cm. Mdi hop cho vao 500 \i\ alkali pha bang (1,7%) va day lai. Mau da xii ly dupc dat trong difiu kifin nhiet dd cua phong thi nghiem trong 30 phiit. Cac hop dugc md ra timg cai theo thu tu, roi danh gia mui thom bang phuong phap ngui.
2.2.2. Danh gia da dang tiieo kieu gen - Ly tiich A\:-lTheo Nguyen Thi Lang (2002).
' Vien Nghien cuu Nong nghiep Cong nghe cao DBSCL
KHOA HOC CONO NGHl
• Phan b'ch da hinh bang ky thuat SSR Mau DNA duoc chon phan tich PCR - SSR theo phuong phap Nguyen Thi Lang (2002).
2.2.3. Chgn loc eac quan the lai thong qua lap ban do GGT
Ki^m tra kieu gen cua quSn the con lai tren nhiSm sdc the so 8 dua tren cac chi thi phan tir da hmh giiia cay bd va me.
Lap ban d6 GGT danh gia sir da dang di duyen ciia qudn the con lai, qua do chpn lpc cac ca th^
mang gen muc tieu mong muon.
Phuang phap GGT do Tanksley va ctv de xuat (1986) va sau do, Van Berllo (2008), Milne va ctv.
(2010) da xay dung phdn m^m him dung nay. GGT
2.0: "graphical genolyping" la phuong phap moi do nhom tac gia ciia Dai hpc Wageningen phat trien, khi do cac alen the hien dong hop trpi. dong hpp lan, di hpp ff tat ca cac con lai trong mpt qudn the, cho phep cong tac chpn lpc cac ca die quy tu nhiing gen mong muon mgt each co hieu qua nhdt.
Phuong phap lap ban do GGT thong qua cac buoc nhu sau:
(1) Lap ffle dii lieu tren Excel: ma hoa gen cua qudn the voi A, B la kieu gen ding hpp tir cua cay bd me; H la kieu gen di hpp tir; U la kieu gen chua dupc xac dinh; (2) Nhap dir lieu vao cua sd GGT: chuydn doi dir lieu Excel sang da lieu GGT; (3) Xu ly sd lieu trong GGT; (4) Dang xudt ket qua.
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Hinh 1. Phan tich GtTT trfin q u ^ th^ lai d cSy lua (Ngudn: Milne va ctv. 2010)
3 . KET QUA UA THAO LUAN ^^'^ ' ^ ^ ' ^' ^^ "^^ ^ ' ^ *"9^ ^^^ ^^^^ Sis niiu 3.1. PhSn tich miii thom
Trfin bd me ghi nhan 10 cay khong thom va bd 10 cay thom dirge chgn lira de ddi chiing. Cac the he
thom ket qua dugc ghi nhan trfin hinh 2.
NONG NGHIEP VA PHAT TRIEN NONG THON - KY 1 - THANG 5/2020
KHOA HOC CdNG N G H £
MUI THaM TREN BO ME VA CON LAI
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a KHONGTHOM •THOM
3.2. Di truydn hirong thom
Cac hat Fi ciia cap lai tir OMCS2000/KhawdawMali 105 dugc cho tu thu Fg. Dir lieu mui thom cho hai quan the Fg va F3 dugc trinh bay trong bang 1. Ty le phan ly F2 khdng thom ddi vdi cay thom la 3:1, dieu nay cho thay sir di truyen cua mgt gen lan duy nhat trong gidng liia thom cho gidng KhaoDawMali 105.
Hinh 2. Su b i ^ dgng mui thom cua bd m^ v i con lai Fg, F3, F4. F7 cua to hpp OMCS2000/KhawdawMaU 105
Bang 1. Ki€u phan ly ciia thg hfi F, F, cua t6 hgp OMCS2000/KhawdawMam 105 The he
F . F:,
Nam 2018 2019
Sd cay 1220 304
Thom 305
76
Khong thom
915 228
Chi Binh Phuong (3:1)
1,028 0,017
G i a t n P 0,20-0,30 0,96-0,99 3.3. Da dgng ngu6n gen trfin cAc gidng liia bd
Vific chgn gidng liia ling dung MAS da dem lai nhimg thanh cong nhat dinh trong thdi gian gan day nhu: nit ngan thdi gian chgn tao, chpn dupc cac gidng khang vdi dieu kien bat lgi, gidng khang bfinh, gidng pham chat (Lang va ctv, 2014 - 2018). Do dd, cac gidng liia bd me (OMCS2000, OM1490; Jasmine 85 va KDML105) dugc phan tich ki^u gen xem xet cd mang gen quy dinh la gen miii thom. 35 chi thi phan tu trfin nhifim sac the sd 8 dugc su dung d^ danh gik da dang di truyen giira cac gidng lila bd me, tuy nhien chi co 21 chi thi phan tir cho da hinh (RM586, RM337. RM52, RM38, RM 25, RM 2126, RM 310, RM 344, RM 72, RM 32, RM 195, RM 3459, RM 210, RM 256, RM 230. RM 264, RM 281, RM 152, RM 547, RM223vaRM3754).
Ml 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4
Theo Nguyfin Thi Lang va ctv (2008), gen miii thom a Ilia dugc kiem soat bdi gen chinh dinh vi trfin nhifim sac the sd 8 lifin kfit vdi chi thi RM223 va RM 3459 (Shu va ctv, 2008). Phan tich b-fin chi thi RM223, ket qua khuech dai PCR cho bang hinh 6 hai kich thudc khac nhau 200bp va 220bp (Hinh 3).
Gidng (1) OMCS2000 cho bang hinh o kich thuoc 200 bp, gidng nay bieu hien khong mang gen miii thom. O kich thuoc 220 bp mang gen mtii thom cua gidng (2) KDML105. (3) Jasmine 85. (4) OM1490 khong ghi nhan min thom. Ddi vdi RM 3459 xac dinh gen muc tifiu cho gen miii thom ghi nhan miii thom trfin KhaodawMali 105 va Jasmine 85 ghi nhan voi kich thuoc 300 bp cho miii thom hai gidng OMCS2000 va OM 1490 ghi nhan khong thom kich thuoc 320 bp.
1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 M
RM223, RM586, RM337, RM52, RM3459, RM25, RM310, RM72, RM32, RM195, RM210, RM256, RM1.52, RM281, RM344
Hinh 3. K^t qua da hinh ciia c^c gidng bd m? vdi c ^ chi thi cho gen lifin quan 15 chi thj phan tu trfin bdn gidng Ilia: 1: OMCS2000,2: KhoaDawmali 105,3: Jasmine 85 v i 4: OM 1490 trfin gel polyacrylamide voi
nhudm nitrat b ^
KHOA HOC CONG NGHE
Diing chi thi phan tu RM223 de xac dinh gen miii thom trong quSn the F4 chgn ra 35 cay d^ danh gia san pham PCR (Hinh 4). Ket qua ghi nhan trfin hinh 4 cho tiiay xuat hien voi hai bang cd kich thiroc 200 bp - 220 bp tuong ung vol bd me cho gen tihom va khong thom. O vi tri band dong sd 4, 17, 18, 19,
29, 20. 31 chi xuat hien mgt bang tuong img v^'i vi tn P2 (KhaoDawMali 105) mang gen min ihoni Trai lai, d-vi tri bang sd 1, 9, 2,10, 3,11,12. 5.13, 6. 14,15, 8, 16, 25, 26, 27, 20,28, 21, 22, 23. 24. 32, 33. 34, 35 xuat hifin 1 bang tirong img v6i vi tri P1(0MCS 2000) khong mang gen thom.
Hinh 4. San pham PCR ciia chi thi phan tu RM223 trfin 35 ddng lifin k^t vdi gene mui thom trfin nhi^m s5c tti^
sd 8, vi tri hai bang 200 bp (OMCS2000) va 220 bp (KhaoDawmali 105), trfin gelpolyactylamide v6i nhudm nitrat b?ic
Ghi chu: 1: OM CS 2000; 2: KhawdawMah 105; 1-35 cay F^.
Ddi voi RM3459: ghi nhan quan the OMCS2000/KhaoDawMali 105 san pham PCR tren hinh 5 cho thay cd 9 cay ddng hop tu cung vdi alen cua KhaoDawMali 105. Cac ca the ovi tri 5, 6, 20, 21, 29, 30, 31, 32. 33 cd ciing kich thudc vdi
KhaoDawMali 105 tuong ung vdi kich thudc 300 bp.
Co 13 ca the o vi tri sd 1. 2, 3, 4. 22, 23, 24,25,26, 27, 28, 34, 35, CO ciing kich thuoc voi OMCS2000 vdi 320 bp. Cac ca th^ cdn lai mang kieu gen di hgp tir cd cung kich thudc voi bd va me (Hinh 5).
Hinh 5. San p h ^ PCR cua chi thi phan tu RM3459 trfin 35 ddng lifin k^t vdi gene miii thom trfin nhifim sdc th^ sd 8, vi hi hai bang 300 bp (KhaoDawmali 105) va 320 bp (OMCS2000). trfin gel agarose 3%
Ghi chii: M: la marker chuan; 1: OMCS2000,2: KhaodawMaU 105,1-35 la cay lai F4.
3.4. Chgn lgc ckc q u ^ th^ hdi giao thdng qua lap ban dd GGT
3.4.1. Chon loc cac ca thd F^ cua quin the lai OMCS2000/KhaoDamiian 105
Quan th^ OMCS2000/KhaoDawmali 105 cho tu thu tiep tuc va chon lgc the he F7. Trong quan the F7 chpn Igc 50 cay de danh gia kieu gen thong qua ban dd nhifim sac the tren timg cay. GGT (Graphical genotyping) la phuong phap cho phep tiie hifin cac alen ddng hgp trgi, ddng hgp lan, di hgp cua mdt quan the. Ban dd GGT giiip dfi dang nhan biet duoc kieu di truyen ciia cac con lai trong quan the cd doan gen so voi bd va me. Ban do GGT dugc xay dung
tren nen tang OMCS2000/KhaoDawmali 105 0 tii^
he F7. Trfin ban do nay, gen quy dinh tinh trang miii thom dugc danh dau bdi 21 chi Ihi phan tii trfin nhifim sac th^ sd 8, khoang each di truyen tir 0-56 cM. Cac ca the con lai dugc lua chgn phai mang gen ddng hgp trgi trfin vimg di truyen nay. Qua ban do GGT d hinh 6 cho thay 4 ddng cd 100% cac viing gen triing hgp vdi bd (KhaoDaw Mali 105), mang gen muc tieu. Cac ca the dugc chgn la ddng 19 (F2-4-6-16- 1-7-19); ddng 20 (F2-17-l-16-l&^20); dong 29 (F2 -18- 3-1-8-106-29); dong 31 (F2-31-7-8-38-10-31). Di^u nay chiing td rang trfin 4 ddng tren chgn lu F, tdi F; da cd su ddng nhat vfi doan gen miii thom tren cac ddng nay. Rieng hai dong 4 (F2-5-100-8-19-4 4) va dong 39
NONG NGHIEP VA PHAT TRIEN NONG THON - KY 1 - THANG 5/2020
KHOA HOC CdNG N G H C
(F2 - 6-1004-1-8-10-39) ghi nhan tren chidm 95,2% co miii thom huong ve bd trong khi dd dong 34 ( F2-1- 100-8-10-6-34) mill thom dat 80% nghieng vd bd. Hai ddng nay tidp tuc duoc chpn tiep the hd F,. Cd hai dong cd 100% mang vimg gen ciia me (OMCS2000) bao gdm ddng 35 {F2-10-S6-7-1835) va ddng 43 (F2- 14-16-100-1-743). Trdn hinh cung ghi nhan cac ddng
phan ly cao nhu dong 26 (F2 -10-9-105-fr826), dong 32 (F2-154-1-1-32), ddng 41 (F2-1-3-7-810541), ddng 42 (F2 -l-105*7-6-42), ddng 47 (F2-2-7-8105.647), ddng 49 (F2-1-8-7-14-1549). Cac dong nay can hep tuc trdng de xem xet va chpn lua nhidu the he hep theo.
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Hmh 6. Sv da dang di truydn th^ h^ F7 trfin qudn tbi lai hdi giao OMCS2000/KhaoDawmali 105 trfin nhiSm s ^ t h ^ s d 8
Chu thich: mau xanh duong: kieu gen theo cay bd (KhawDawMah 105), mau do: kieu gen theo cay me (OMCS2000), man xam: kieu gen di hgp td, khung mau xanh la cay: danh dau cac ca the dugc lua chon, 1-50:
cac cathScda quan tiie OMCS2000/KhaoDawmali 105.
3.4.2. Thanh phan nang suat va nang suat
TT
1 2 3 4 5 6 7 8 9 10 11
Ten ddng
19 (F2-4-6-16-1-7-19) 20 (F2-17-1-16-19-820) 29 (F2 -183-1-8-106-29) 31(F2-31-7-838-10-31) 4 (F2-S100-81944) 39 (F2 - 6-100-4-1-810-39) 35 (F2-10-8-6-7-1835) 43 (F2-14-16-100-1-743) 34 (F2-1-100-810-6-34) KHaoDawMali 105 tddi chung)
OMrssnon iddi chiing) CV(%)
Chieu dai bdng (cm) 25,35 ab 25,84 a 24,08 be 24,57 abc 23,89 c 23,64 c 23,73 c 24,64 abc 23,75 c 24,51 abc 24,02 be 24.37 5,8
Sdhat chdc/bdng
(hat) 105,50 a 85,70 ab 88,40 ab 87,10 ab 81,50 ab 74,10 b 71,60 b 89,90 ab 92,40 ab 73,30 b 99,40 a 84,45 25,3
Ty le hat lep
(%)
22,34 b 27,42 ab 24,23 ab 24,95 ab 25,53 ab 26,25 ab 27.70 ab 24,96 ab 20,86 b 22,24 a 22,87 ab 25,4 25,6
Khdi lupng 1000 hat
(g) 27,32 a 27,43 a 27,52 a 27,03 a 27,63 a 26,22 a 27,43 a 26,91 a 27,51 a 2883 a 26,02 a 27,26 8,8
Nang suat
g/bui 18,31 abc 13,33 c 22,43 a 16,22 be 16,75 be 15,63 be 15,10 be 18,89 abc 19,26 ab 14,47 be 18,64 abc 17,18 31,6
g / l m - 604,10 abc 439,96 c 740,32 a 535,13 be 552,82 be 515,63 be 498,27 be 623,21 abc 635,45 ab 477,51 be 615.02 abc 567,04 31,6 Ki^m h-a cac dong tiiom va khong
ngoai ddng de danh gia nang suat va thom trong thanh phan
nang suat trong vu dong xuan 2018-2019. Ghi nhan cac ddng cho nang suat cd y nghia. Thanh phan nant,'
KHOA HOC C 6 N G N G H i
suat va nang suat cua cac ddng dupc ghi nhan hen bang 2 cho thay: Chieu dai bdng hung binh dia cac to hpp lai la 24,37 cm. Trong dd, ddng 20 (F2-17-1-16- 19-8-20) cd chidu dai bdng dai nhit la 25,84 cm. Sd hat chac/bong cua cac ddng dao ddng tii 71,6 den 105,5 h a t Trong dd, ddng 19 (F2-4-6-16-1-7-19) cd sd hat chac/bdng cao nhat 105,5 hat. Ty le hat lep trung binh cua ddng la 25,4%. Khdi lupng 1000 hat dung binh c i a cac td hpp lai la 27,26 g. Khdng co su khac nhau ve khdi lupng 1000 hat giiia cac td hpp lai.
Nang suat ciia cac td hop lai trung binh la 567,04 e/m', td hpp lai 29 (F2 -183-1-8106-29) cd nang su&
cao nhiit la 740,32 g / m l Hau het cac gidng cao hpn bd la KhaodawMali 105.
4.1KA0UIAN
Cac gidng liia thpm tda ra min thom tir la, hat va liic ra hoa d cac giai doan tnrdng thanh khac nhau.
Mpt sd thanh phan hda hpc da dupc chiing minh la rat quan trpng ddi vdi mui thom cua gao nhu hpp ch^t 2- acetyl -1- pyrroline (2AP) la thanh phan chinh ciia mill huong gao (2AP). Wongyhchai etal. 2003).
Sir tich liiy 2AP trong cac kidu gen thom cd the dupc giai thich bang su hien dien cua cac ddt bien din den mat chirc nang cua siin pham gen ^ (Bradbury et al, 2005). Huong thom trong gao la mdt dac tinh cam quan va trong nghien cihi cua Lang va ctv (2016), cac nh^ chpn gidng da sir dung thuat ngir ma hp quen thudc de md ta miii ciia gao thpm. Dieu can thiet che cac nha tap gidng liia la phat trien danh gia dang tin cay, nhanh chdng va hidu qua chi phi che miii hupng gap trong cac chuong trinh chpn tao gidng. Vi chat lupng cam quan ludn dupc xem xet quan trpng trong cai tidn liia gap, da sir dung cac phupng phap cam quan phan giai miii thpm til la bang KOH va hat nhai de nhan biet cd miii thpm. Tuy nhien, viec phat hien mdi thom bang phuong phap earn quan hoac hda hpc khdng cd nghia la thuc te khi xir ly sd lupng ldn mlu.
De giam thieu anh hudng ciia dieu kien mdi hirdng va cae ydu td khac, viec xac dinh min thom h-ong nghien cuu cua chiing tdi da duoc thuc hien hen quan the Fg. Cay F. cd mill thom cao (nghia la hrong tu nhu gidng bd KhpaDawMah 105) dupc xac dinh bang hai phuong phap cam quan da ket hpp de phan tich di huyen vdi hai chi thi phan tii RM223 va RM 3459 cho da hinh hen ca hai bd me OMCS2000 va KhaoDawMali 105. Didu nay giiip hen lpi cho hep hic nghidn cuu h-en ban dd GGT. Tren ban dd GGT da dupc tach rat ro viing mang gen gidng bd va gen gidng me thd hien d thd he F,. Trong thd he F; nay
cac vimg cdn phan ly chiem kha cao nhu ddng 26 (r2 -10-9-105-6-826), ddng 32 (F2-1&4-1-1-32). ddng 41 (F2-1-3-7-810541). Ddi vdi cac ddng thuan cOng danh gia hnh hang nang suat va thanh phan nang suit. Cac gidng cd miii thom va nang suat cao nhu ddng ddng 29 (F2 -183-1-8-106-29) va ddng sd 19 (F2- 4-6-16-1-7-19). Didu nay gdp phin cho viec chpn gidng h-ong cac ddng nay h-ong tuong lai.
S. KET LUAN
Da xac dinh dupc su cd mat gen lan quy dinh miii thom d cay liia h-ong quan the F^ va F3 cua td hop lai OMCS2000/KhaoDawMali 105. Cd su da hinh h-en hai chi thi flii dinh vi mui thom la RM223 va RM 3459 hen nhitm sac the sd 8. Dua vao ban do GGT giiip dd dang nhan biet diroe kieu di truydn ciia cac con lai hong quan flie cd doan gen so vdi bd va me h-ong the he F7 cua td hpp lai OMCS2000/KhaoDawMah 105. Thdng qua 21 chi flii phan tir hen nhidm sac the sd 8, doan di truydn vdi khoang each hr 0-56 cM. Cac ca thd con lai dupc lua chpn phai mang gen ddng hpp trdi tren viing nhidm sic the tuong ling thu dupc 4 ddng cd chiia 100%
viing gen triing hop vdi ca the bd (KhaoDawMali 105), mang gen muc hdu mui thom. Cac ca the duoc chpn cd mill thom la: ddng 19 (F2-4-6-16-1-7-19);
ddng 20 (F2-17-1-16-19-820); ddng 29 (F2 -183-1-8 106-29); ddng 31 (F2-31-7-83810-31). Ddng cho nang suat cao nhat la ddng 29 (F2 -183-1-8106-29). Day la kdt qua ndi bat quan trpng cho vide chpn gidng liia cd mill thpm hong tuong lai.
LOI CAM ON
Nhom tac gia earn an chuang trinh Tay Nam bo da cung cap lanh phi de thuc hien de tai nay.
TAI UBI THAM KHAO
1. Bradbury MT, Fitzgerald TL, Henry RJ, Jin QS and Waters LE (2005). The gene for fragrance in rice. Plant Biotechnol J 3:363-370.
2. Hang YJ, Uu YB, Rao ZX and Pan XY (1995).
Studies on inheretance of aroma characters of scented rice. Achi Agriculhirae Jiangxi 7:8893.
3. Lorieux, M., Petrov, M., Huang, N., Guiderdoni, E., Ghesquiere, A. (1996). Aroma in rice:
genehc analysis of aquantiuthve halt. Theor Appl
Genet 93, 1145-1151.
hrtps://doi.org/10.1007/BF00230138.
4. Li JH, Wang F, Uu WG, Jin SJ and Uu YB (2006). Geneflc analysis and mapping by SSR marker for fragrance gene m rice Yuefeng B. Moi Plant Breed 4:54-58
KHOA HOC CONG NGHE
5. Milne et al.. Mihie 1, P Shaw, G Stephen, M Bayer, L Cardie, WTB Thomas, AJ Flavell and D Marshall (2010). Flapjack—graphical genotype visualizaUon. Bloinformatics, 26: 3133-3134.
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APPUCATION MARKER ASSISTED SELECTION IN BREEDING IMPROVEMENT FORFRAGRANT GENE ON RICE Oiyzsa sativa. L
Ha Ngoc Hien, Nguyen Thi Khanh Tran, Nguyen Thi Lang, Bui Chi Buu Summary
The rapid development of rice marker technology, great progress has been made in understanding the fragrant gene in nee for export, and a series ol funcdonal markers has been developed for screening the aroma gene and breeding new rice vaneties in recent yeai^. This paper mainly apply GGT from popualbon F7 from OIVICS2000/KhaoDawMali 105, of diefragranl gene, and the application of aroma gene functional markers in the genetic improvement and breeding of aromatic nee. Populations OMCS2000/KhaoDawMah 105 are noted as recessive genes on populations F2 and F3. Tliere is a polymorphic on the two only aroma positioning lest is 1^223 and RM 3459 on chromosome 8. According to the GGT map, it is easy to identify the genetic pattern of the hybrids in populations compared to the genomic offather and mother in the generation OMCS2000/KhaoDawMalI 105 in the F 7 generation. Adopted by 21 molecular SSR on chromosome number 8, die genetic distance fi-om 0-56 cM. The selected individuals must cany a superior co-gene on a chromosome that has 4 individuals with 100% of the genes that coincidize with (KhaoDawMali 105), canymg a fragrant target gene. The selected Imes as: line 19 (F2-4-6-16-1-7-19); line 20 (F2-17-i-16-19- 8-20); line 29 (F2-183-1-8-106-29), line 31 (F2-31-7-8-38-10-31). Thus, it provides unportanl references for tile cultivation of new varieUes of aromatic nee.
Keywords: Application, genetic breeding, GGT. Iragrantrice. marker Ngudi phin bidn: GS.TSKH. Trin Duy Quy
Ngly nh|n bai: 28/02/2020 Ngly thdng qua phan bi^n: 30/3/2020 Ngly duydt dang: 6/4/2020
