KHOA HOC CONG N G H l
IVGHIEIM CLTU HIEU QUA PHOIVG TRIJ
Fusarium axysparum GAY HAI CA PHE CLIA CHAT CHIET XUAT VD QUE KET HOP VOI CHITOSAIM
Nguydn Ddng Mmh Chdnh'. Luong Thi Hoan^ Nguyen Xudn H6a^ H 6 Phiic Nguyfin*
T6MTAT
Nghien cuu diroc thuc hien voi muc lieu danh gia hieu qua phong trir nam Fusanuni o.xysporum gay h^i cSy ca phe cua chd pham sinh hoc (CPSH). Thi nghiem diroc thuc hien tren vuon ca phe kinh doanh 8 nam Uioi, bo tri Ibeo kieu khoi ddy dii ngdu nhien 1 yeu td (RCBD) gom 4 cong thirc, 3 lan Igp. moi 6 co so 9 cay cd phe, giira cdc 6 co so c6 dai phan each la 1 hang ca phe Mot lit CPSH duoc hoa lan trong 500 lit nude tuong ung voi nong dp 0,2%. Bdn cong thiic thi nghiem bao gdm: Khong xii ly che pham sinh hpc (doi chiing);
CPSH 0,2% (3 lit/cay); CPSH 0,2% (5 lil/cay); CPSH 0.2% (7 lit/cay). Ket qua cho diay, ch4 pham smh hoc CO lac dung han che ly le cay bi vang la va chi sd hi benh tren cay ca phe. Dac biet sau 3 thang xii ly CPSH, cong thirc 3 (5 lit/cay) va cong thuc 4 (7 lit/cay) ty le cay bi vang la giam co y nghia so voi cong thirc 1 (khong xii ly). giiia cong thirc 3 va cong thiic 4 khong co su khac bi?l thdng ke. Chi sd b?nh o 3 cong thiic CO xu ly CPSH giam co y nghia so voi cong thiic ddi chiing. Xii ly cb£l pham smh hpc gidm dang k^ benh vang la, ty 16 u sung va thoi r& Q-en cay ca phe so voi cong thiic ddi chimg. Tir nhung ket qua tren co th^
khuyen cao sir dung che pham sinh hoc voi thanh phan chinh Id chat chi^t xudt vo que va chitosan dd phong trir hieu qua /i/s5/7i/mo.vj'S/)on//ngay hai tren cay ca phfi.
Tu kh6a: Che'pham sinh hoc. chat chiet xuat vo que. chitosan. Fusarium oxysponim.
l.fiATVJlNBE
Tdy Nguyen Id vimg cd dien tich vd san lupng Idn nhat ca nude vd cd phe {Coffea canephora). Dien hch ca phe chidm 89,4% so vol tdng dien hch ciia ca nude [8]. Hau het ngudi ndng dan d viing nay sdng dua vdo ngudn thu nhap tir cay hdng ndy. Vimg cd phe Tdy Nguyen dang diing trudc nguy ca gid cdi, hien tai cd khoang 100.000 ha nang sudt dudi 1,5 Idn/ha, edn hdng tdi canh. Thuc te hien cd nhieu didn tich tai canh khdng thanh cdng, nguyen nhdn chinh la van de sdu benh hai, dac biet Id tuyen trung va nam benh. Fusarium sp. Id mdt lodi ndm gdy benh vdng Id trdn ca edy con vd cdy ca phe kmh doanh, ndu ndm benh nay tdn cong cdy ca phe se smh Irudng phdt hien kem, nang sudt va chat lupng giam ro ret. Ngudi ddn vdn cd xu hudng sic dung thude hda hpc dd phdng trir nam gdy hai cdy ca phe. tuy nhien su dung thude hda hgc gay anh hudng nghiem trpng cho nguoi su dung, chat luong san pham gidm
' \'ien Cay luong thuc va Cay thuc pham
" \'ien Duoc lieu
•" Vien Khoa hoc Ky Uiual Nong Lam nghiep TayNguyen
•* Cong u TNHH Thuong mai va Djeh vu Qu6c te TipTo Ma Lai '
Email, [email protected]
vd d nhidm mdi trudng do du lupng ciia thuoc hda hoc delai [1].
Nghidn cuu trudc ddy cho thay chat chiet xuat tir vd cay qud cd hdm ndng phdng trir hieu qua nam Rhizoctonia solani gdy hai cdy hdng [6] vd Inyen triing Meloidogyne incognita [5j. Ben canh dd chitosan da dupe nhieu nghien cuu chiing minh Id hpp chat cd kha ndng phdng trir nam benh tidm ndng. Chitosan vdi hoat tinh uc che su nay mam cua bdo tu nam, su smh hudng phat trien cua spi nam Phytophthora capslci va Altemaria solani [9], Fusarium [3], Alternana kikuchiana vd Physalospora piiicola [4]. Vi vdy nghien ciiu nay nham ddnh gia hieu qua phdng trir cua chd pham smh hpe la hdn hpp dup-c phdi hdn gitra chat chidt xuat vd que va chitosan cho viec phdng trir nam benh Fusanum sp.
gay hai hen cay ea phe hong dieu kien ddng rudng.
2. VAT UEU VA PHUONG PHAP NGHei CUU 2.1. vat li$u nghidn cuu
• Che pham sinh hpc Bade Cmsan gom chat chiet xudt tir vo que 16% + 3% chitosan trong 1 lit che pham.
+ Vd que cd ngudn gdc tu huyen Tra My, tmh Qudng Nam.
NONG NGHIEP VA PHA 2/2020
KHOA HOC CONG NGHE
+ Chitosan (1.000 - 3.000 Da, chiing nhdn phan tich kem theo) co ngudn gdc tir Cdng ty Sokcho Mulsan, 76 Nonggongdanji-gil, Sokcho-si, Gangwon- do 24899. Han Qudc.
- Nam bdnh Fusarium oxysponim: Duoc phdn lap tu cdc rd cay cd phe vdi bi benh hdn mdi trudng PDA theo phuong phap cua Burgess etai. (2008) [2].
2.2. Phirong phdp nghidn ciiu
Chiet xuat vd que bdng dung mdi methanol: Su dung theo phuong phap ciia Nguyen etai. (2012) [5].
Mau dupc say b nhiet dp 50"C trong vdng 3 ngdy, sau do cdt nhd 2 - 3 em, cho vao binh (the tieh phu thude vao sd lupng mdu) vd mdu dupc chiet xudt bdng methanol (80%) vdi ty Id vo que/methanol Id 1/5 theo the hch. Sau dd cho vdo may de ldc vdi tdc dp 150 vdng/phiit. Sau 3 - 5 ngdy, dich chidt xuat se dupe Ihu bang cdch Ipc sach, sau dd cho methanol 80% vdo tiep hdn hanh tuong tu sau 3 ngdy thu tidp dgt 2.
Djeh Ipc dupc cd dac bdng each sir dung mdy bdc hoi chan khdng (Eyela N-1000) b nhiet dp 40"C dd loai bo hoan loan lupng methanol.
Thanh phdn ciia che pham sinh hpc (CPSH) gom chat chidt xudt vd que (16%) theo the tich vd chitosan (3%) theo khdi lupng.
Bd hi thi nghidm: Thi nghiem gdm 4 cdng thiic, 3 ldn lap, mdi d co so' 9 cdy ca phd (ca phe kinh doanh 8 nam tudi). Cac cdng thirc dupc bd tri theo d vuong, gitita cdc d co sd cd dai phan each Id 1 hang ed phd. Cdng Ihirc 1 (CTl): Khdng xii ly (ddi chiing);
Cr2; CPSH 0,2% (3 lit/cdy); CT3: CPSH 0,2% (5 lit/cay); CT4: CPSH 0,2% (7 lit/cay).
Phuong phdp xii ly: Che pham duoc xu Iy khi dat dll am, xii ly 1 Idn. Tudi dung dieh thude hode nude Is theo cdc cdng thiic thi nghiem vd tudi udt deu xung quanh vimg rd cdy cd phe. Giir ddt dii am sau khi xir ly thude.
Phuong phdp didu ha va ehi tieu theo doi:
- Ty Id va chi sd benh vdng la (%): Mdi d co sd Iheo doi todn bp sd cdy, dem sd cay bi benh vdng Id va phdn cap benh ddi vdi cac cdy bi benh vdng Id do bi hai vimg rd. Mirc dp cay bi vdng Id dupc phdn thanh 5 cdp: Cap 0: Khdng benh (hoac khdng vang la); cap 1: Cdy sinh trudng kem, cd < 25% Id vang hoac rung; cap 2: Cay co > 25 - 50% la vdng hoac ning; cap 3. Cay cd > 50 - 75% Id vang hoac rung; cdp
^' Cay cd > 75 Id rung hoac todn edy bi heo.
+ Ty le vdng Id frL\T,) dupc tinh theo cdng thiic:
TLVL (%) = (Tdng sd cdy bi vang la / Tdng sd cdy didu tra) xlOO.
T Chi so cdy vang Id (CSVL) dupc tinh theo cdng thiic:
CSVL chi sd vang la - Tdng cua tich sd giira cdy bi benh vdi cdp benh tuong img.
- Ty le cdy chet (TLCC) % = (Tdng sd cay chet / Tdng sd edy didu ha) x 100.
- Mat dd nam benh gay hai hong dat (CFU/g dat) va tan suat xudt hien nam hong rd cd phd (%):
mdi cdng thiic lay mdu dat vd re d- 3 edy cd dinh eua mdi d ca scr, trpn deu thanh 1 mdu. Vi tri Idy mdu dat vd rd d tdng dat 0 - 20 cm, lay hong khu vuc hinh chidu tan la (xung quanh khu virc dupc xir Iy thude). Khdi luong mau dat Id 1 kg, khdi Iuong mau rd Id 100 g.
- Hieu lire cua thuoc dupc tinh theo cdng thiic Henderson-Tilton.
Thdi gian theo doi: trudc xii ly va sau xir ly 1, 2, 3 thdng.
Cac sd heu phdn tich dupc tdng hpp vd xu Iy bdng phdn mdm Excel va SAS 9.1. (SAS. 2004). Cdc gia hi trung binh dupc gdn cac ky tu gidng nhau tren cimg mpl cdt la khong sai khac cd y nghia thdng ke.
2.3. Thdi gian vd dia di^m nghidn ciiu Nghien cim duoc thuc hien tii' thang 01 nSm 2018 den thang 01 nam 2019 tai Vien Khoa hge Ky thuat Nong Lam nghiep Tdy Nguyen.
3. KFT QUA NGHEU CUU VA THAU LUAlU
Bang 1 cho thdy: Trudc khi xir Iy che pham, vudn ca phe dd bi nhidm benh va ly le benh tuong doi ddng ddu. Sau khi xii ly CPSH vdi ede ty le khde nhau eho kdt qua phdng trir khde nhau. Che pham sinh hoe cd tdc dung han ehe ty Id edy bi vdng la va chi sd bl benh tren cdy cd phe. Dac biet sau 3 thdng xir ly CPSH, cdng thiic 3 (5 lit/cay) va cdng thirc 4 (7 ht/cay) ty le cdy hi vang Id gidm cd y nghia so vdi cong thiie 1 (khdng xu ly), giiia cdng thiic 3 vd cdng thiic 4 khdng cd su khac biet thdng ke. Chi sd benh d- 3 cong thtic cd xu Iy giam cd y nghia so vdi cdng thiic dot chiing. Hieu luc phdng benh vdng Id ciia cd phe do nam Fusanum oxysponim cung dupc the hien d-hinh 1.
"^N-KY 1 + 2 - T H A N G 2/2020 159
KHOA HOC cdes® ^@se£
Bang 1. Anh huong cua che pham sinh hpc dky ^ 1^ cdy bi vdng Id vd chJ sd bi bgnh trdn cay ca phd Cong
thiic 1 2 3 4 LSD„a,
CV%
TXL(%) TLB 35,3 34,8 34,1 34.1 3,91 4.32
CSB 11.0 10,6 10,0 9.6 2,63 9,77
SXL 1 than? (%) TLB 52,5"
38,5'' 36,2' 25,0'- 3,85 3,87
CSB 26.7' W.l' 10.4'"
9.8-"
2.55 6.73
SXL 2 thanfi (%) TLB 56.5"
43.4' 35.6' 33.8' 9,55 9.29
CSB 26.2' 16.8' 13.3'"
9.5' 7,14 16,6
SXL 3 thani; 0 ' TLB 62,3' 34.5'' 22 9' 18.6' 10.03 11.09
CSB 23.8 13.1"
9.5' 7 2' 8.01 22.95 Ghi chu: CTl: Ddi chung; CT2: Che pham smh hoc 0.2%31/cay; CT3: Che phdm sinh hoc 0.2% 51/cay;
CT4: Che'pham sinh hpc 0.2% 7I/cay; 7XL- tiirdcxuh": SXL sau xuly; TLB: Ty le benh: CSB: chi sd benh.
Hmh 1. Cdy cd phd khdng xu ly CPSH (A) vd cdy cd phd c6 xu ly CPSH ndng dd 0,2% vdi 7 lit/cdy (B) Bang 2. Hidu lire phdng trir bdnh vang la hdn cay cd phd ciia ch^ phdm smh hpc Bacte Cinsan (%)
Cong thirc 1 2 3 4 LSD„a,
CV%
Ty le eav bi van}^
SXLl thang C 29.5' 30.6' 59.6"
14,52 10,99
SXL 2 thang 0"
21.9'- 54.1' 58.9"
12,97 15,88
la SXL 3 thang
(>
45,3' 66,4' 69,6' 14,12 11.92
Chi so vang la SXLl
thang 0 58,3 65,9 66,8 AS 14,01
SXL 2 thang 0' 52,5' 60.7"' 72.9"
15,26 12,54
SXL 3 thang 0' 52.2' L 67.7"'
80.0"
16,54 12.66 Ghi chd: CTl: Ddi chung: CT2: Chi pham smh hoc 0,2% 31/ cay: CT3: Chi pham sinh hoc 0,2% 51/ cay:
CT4: Chi phim smh hoc 0,2% 71/cay TXL: budc xuly SXL sau xuly.
Bang 3. Anh huong ciia ch^ pham sinh hoc iia mat dp Fusarium oxysponim trong Ait (CFU/g dat) va tan suat xnat hi^n nam trong r^
Cong thiic 1 2 3 4 LSD,.„
CV%
TXL(%) MDN 0.23 X 10"' 0.24 X 10*
0.24 X lo' 0.27 x i o '
TSN 53,3 53,0 53,1 52,1
SXL 1 thang (%) MDN 0,31 X lo' 0,30 X lo' 0,27 X l o ' 0.24 x lo' AS
1 S.27 ,
TSN 50.3"
42.6"
40.5"
28.4' 11.69 11.06
SXL 2 thang (%) MDN 0.24x10*
0.21 x l o ' 0,18x10*
0.14 X l o ' TSN 50.3' 42,7"
23.7' 22,0' 13.78 15,2
SXL 3 thing ('»
MDN ! TSN 1.28 X 10' , 39.8"
0,72 x i o ' 1 33,5' 0,65 x i o ' 19,5' 0.63x10' 16.4' 11 .'ii Hi 17 ' Ghi chu: CTl: Ddi chdng: CT2: Chi pham sinh hoc 0,2% 31/ cay; CT3: Chi phim sinh hoc 0,2% 51/ cay;
CT4: Che phim sinh hoc 0,2% 71/ cay: TXL: diidc xu ly: SXL: sau xd ly: MDN- .Mat do nam: 1\\: Tin suit xuit bien.
160 NONG NGHIEP VA PHAf I n A w n TUON - KY 1+ 2 - THANG 2 / 2 0 2 0
KHOA HOC CdNG NGHl
Hi^u lire phdng hir benh vdng Id do nam Fusarium oxysponim hen cay cd phe ciia CPSH dupc thd hien hong bang 2. Ty le cay bi vdng la ciia ca phe sau 1, 2 vd 3 thang xii Iy cd su khac biet ed y n^ia giira cdc cdng thirc. Hieu lue phdng tni gan 7096 sau 3 thang xii ly d cdng thue 4 vd 66,4% d cdng thuc 3, khde biet co y nghia so vdi cdng thiic ddi chiing vd cdng thiic 2. Hieu luc cua CPSH sau 3 thang xii ly d cdng thtic 4 cao hon ed y nghia so vdi cong thiic 2 vd cdng thtic doi chimg. Dieu ndy cho thdy xir Iy CPSH ndng dp 0,2% vdi 7 lit/cdy cd phe cho hidu luc phdng trir Fusanum oxysponim hieu qua nhdt.
Bang 3 eho thay mat dd nam trong ddt vd tan suat xuat hien nam trong re trudc thi nghiem d" cdc cdng thuc tirong duong nhau. Sau khi xu ly che pham sinh hpc 1. 2 va 3 thdng, mat dd ndm hong ddt b cde cong thirc xii ly cd xu hudng giam so vdi cdng thiic ddi chimg tuy nhien su khde biet khong cd y nghia thdng ke. Trong khi dd tan suat xuat hien ndm b cdc cdng thiic ed xti Iy CPSH giam cd y nghia so vdi cong thue ddi chung. Sau 1 thang xu ly, cdng thuc 4 giam cd y nghia so vdi cdc cdng thuc cdn lai.
Sau 2 vd 3 thdng xii ly cdng thtic 3 vd 4 giam cd y nghia so vdi cdng thiic 1 vd 2. Didu ndy cho tliay CPSH cd hieu qua ldm giam tan suat xuat hien Fusarium oxysporum hong re sau 2, 3 thang xii Iy d cong thiic 3 va 4.
Bang 4. Hi^u luc phong tru Fusarium oxysporum hdn cdy cd phd (%) Cong
thiic 1 2 3 4 LSD„x
cn
Ty ie cay bi vang SXL 1 thang
0'- 18,5' 24,5' 40.7' 15..54 28,28
SXL 2 thang 0' 26.9' 31.5' 50,9' 9,52 13,33
la SXL 3 thang
0' 39.5' 47.0' 71.0' 14,08 13,67
Chi sd vang la SXL 1 thang
0'' 24.4' 47,0' 73,2"
9,61 10,01
SXL 2 thang 0' 28,7' 48,3' 75.4' 11,74 11,96
SXL 3 thang 0'' 46,7' 52.7' 78,8' 9,71 8,33 Glli chu: CTl: Doi chung: CT2: Chd pham sinh hoc 0,2% 31/ cay; CT3: Chd phdm sinh hpc 0,2% 51/ cay;
CT4: Che phdm sinh hoc 0.2% 71/ cay; TXL: trudc xu ly; SXL: sau xu ly: MDN: Mat dp nam; TSN: Tdn suat sudt hien.
Hieu luc phdng trir Fusarium oxysporum the hi^n qua sd heu d bang 4 vdi 2 chi tieu hieu luc ciia CPSH ddi vdi ty le cdy bi vang la vd chi sd vang la eay ca phe, Sau 1, 2 va 3 thang xir ly hieu luc ciia CPHS d^n ty Id vdng la o" cdng thitc 4 eao hon ed y nghia so
Bang 5. Anh hudng ciia chd pham sinh hpc ddn ty Id u simg vd thdi rd (%)
vdi cdng thirc 2, 3 vd cdng thtic ddi chiing. Hieu luc phdng trir ciia CPSH ddi vdi chi tieu chi sd vdng Id hen edy cd phe cd su khac biet cd y nghia giira cdc cdng thirc, sau thdi gian xir ly 1, 2 vd 3 thdng hieu luc CPSH giua eac cong thiic luon ed sir khde biet.
C6ng thirc 1 2 3 4 LSD„„
cn
Then gian xu ly TXL
49,0 48.9 48.7 47.5 12,36
NS
SXL 1 thang 55,3' 48,5"
41,3"' 33,5' 14,13 12,11
SXL 2 thang 60,0' 45,1' 36.7'' 29.5' 10,32 9,22
SXL 3 thang 61,4"
43,5' 36.8'«
24,6' 13,23 12,18
Ghi dm: CTl: Boi chdng: CT2: Che pham smh hoc 0,2% 31/ cay: CT3: Chi phim sinh hoc 0,2% 51/ cay:
CT4: Chi pham suih hpc 0,2% 71/cay: TXL tivdcxuly: SXL: sau xuly
' ' N - K ' i ' 1+2-THANG 2/2020 161
KHOA HOC COI^'^ '.^^z
Bang 5 cho thdy, truoc khi xir Iy CPSH vudn cdy cd phe da bi nhiem benh va ty le tuong ddi dong deu giiia cac cdng thuc thi nghiem. Sau xu ly 1,2 va 3 thdng ty le u sung vd thdi rd a cdng thiic ddi chting cd xu hudng tang len, tuy nhien cac cdng thiie cd xir Iy CPHS, ty Id u sung va thdi rd da gidm cd y nghia thdng kd, khde biet ro nhat Id cdng thuc 4 Id 33,5, 29,5 vd 24,6% so voi 55,3, 60,0 vd 61,4% d cdng thirc ddi chting tuong iing sau xir ly 1, 2 vd 3 thdng.
Bang 6. Hidu luc phdng trCr ciia ch^ ph^m sinh hpc ddn ty Idu sung vd thdi r& (%) Cong
thiic 1 2 3 4 iS»o.»
cn
Thai gian xii ly SXLl
thang 0' 26.6' 32.8' 54,5' 10,32 13,86
SXL 2 thang 0' 42.8' 65,4' 75,2' 8,73 7,29
SXL 3 thang 0*
54,3' 67,0' 84,4' 7,01 5,73 Hieu luc phdng trir ctia CPHS den ty le u sung vd thdi rd Id chJ tieu quan ti'png de ddnh gia hieu qua cua CPSH len nam benh Fusarium oxysporum gay hai cdy cd phe. Hieu luc phong trir ciia eae cong thirc khac nhau cd y nghia thdng ke ed ve sd luong vd thdi gian sau xir ly. Sau xir ly 2 va 3 thdng, hieu lire phdng trir d cdng thiic 4 dat gid trj cao nhat la 75,2 vd 84,4%.
4. KET LUAN V A B E N H 4.1. Kdt ludn
Cdc cdng thirc xu ly che pham sinh hpc Bacte Cinsan eho thay cd hieu qud phdng hir nam benh Fusarium oxysporum. Trong dd, cdng thuc xu ly chd pham sinh hpc 0,2%, 7 ht/gdc ca phe cd hieu luc rat cao phdng trir ndm benh Fusarium oxysponim (84,4%) gay hai rd cdy ed phe.
4.2. D^ nghi
Sir dung che pham sinh hoc Bacte Cinsan, thdnh phan ehinh gdm chdt chidt xudt vd que vd bot chitosan ndng dd 0,2% (1 ht che pham hda trong 500
lit nude) vd tirdi 7 ht/gdc dd kiem sodt ndm. giam l\' le u sung vd thdi rd tren cay cd phe.
Tien hanh thir nghiem them heu lupng tren dien rpng de cd co sd ket ludn hieu qua hon.
UMCAMON
.\hdm tac gia xin chan thdnh cam on Quy Ddi mdi Cdngnghd Qudc gia (\ATIF) da cap kinh phf tiiuc hien de tdi ma sd NATIF.TT.04.DT/2017 dd thuc hien nghien ciiu nay.
TAI LEU THAM KHAO
1. Akhtar Y, Yeoung R, Isnian MB. (2008).
Comparative bioactivity of selected extracts from Mehaceae and some commercial botanical mseeticides against two noctuid caterpillars, Trichoplusia ni and Pseudaletia unipuncta.
Phytochem. Rev. 7: 77-88.
2. Burgess LW, Knight TE, Tesonero L, Phan HT. (2008). Diagnostic manual for plant diseases in Viehiam, pp. 126-133, ACIAR, Canberra.
3. Eweis M, Elklioly SS, Elsabee MZ. (2006).
Anhfungal efficacy of chitosan and its thiourea derivahves upon the growth of some sugar-beet pathogens. Int. J, Biol. Macromol. 38:1-8.
4. Meng X, Yang L, Kennedy JF, Tian S. (2010).
Effects of chitosan and oligochilosan on growth of two ftmgal pathogens and physiological properties in pear fruit. Carbohyd. Polym. 81. 70-75.
5. Nguyen DMC. Seo DJ, Kim KY, Kim TH, Jung WJ. (2012). Nematode-anhigonishc effects of Cmnamomum aromaticum extracts and a purified compound against Meloidogyne incognita.
Nematology 14(8): 913-924.
6. Nguyen VN, Nguyen DMC, Seo DJ, Park RD, Jung WJ. (2009). Anhmycotic activities of Cinnamon- denved compounds against Rlnxoctonia solani in vitro. BioControl 54: 697-707.
7. SAS, 2004. SAS/STAT User's Guide, version 9.1. Cary, NC, USA, SAS Institute.
8. Tdng cue Thdng kd, 2015.
9. Xu JG, Zhao XM. Han XW, Du TG. (2007).
Anhfungal activity of oligochilosan against Phytophthora capslci and other plant pathogenic hmgi in viho. Peshc. Biochem. Phys. 87: 220-228
162 N O N G NGHIEP VA PHAT " " ^ ' J ^r^^G. THON - KY 1+ 2 - THANG 2/2020
KHOA HOC CONG NGHE
EFFECTIVENESS OF BIOPREPARATION FROM CINNAMONAND CHTTOSAN RESISTANT TO Fusarium oxysporum DAMAGING COFFEE
Nguyen Dang Minh Chanh, Luong Thi Hoan, Nguyen Xuan Hoa, Ho Phuc Nguyen Summary
TTiis study was earned out to evaluate the effectiveness of biopreparalion against Fusanum oxysporum m coffee. The experiment was conducted on an 8-year-old coffee, arranged m a randomized complete block design (RCBD) with 4 formulas, 3 rephcaUons. 9 coffee Uees per plot, between the plot is separated by a row of coffee. One liter of biopreparalion is dissolved in 500 hters of water corresponding lo a concentraUon of 0.2%. Four experimental treatments include' no treatment biopreparalion (conUol): biopreparahon 0.2% (3 liters/tree); biopreparabon 0.2% (5 liters/tree): biopreparaOon 0.2% (7 liters/tree). The results showed Uial the biopreparabon have the effecl of hmiUng the yellow leaf disease rate and index of coffee trees.
Especially, treaUnent of biopreparabon after 3 months, formula 3 (5 liters/tree) and fomiula 4 (7 liters/tree), the rale of yellow leaf disease significanOy decreased compared to fonnula conb^ol (no treatment), There was no stabsbcal differences between fomiula 3 and formula 4. The disease index in treatments reduced significantly compared to the control. Treatment of biopreparabon have significanUy reduced yellow leaf disease and root rot in coffee compared to the control. These results suggest that using biopreparaUon wilJi the main ingredient of cinnamon bark extract and chitosan to effectively prevent Fusarium oxysporum harming coffee trees.
Keywords: Biopreparabon, cinnamon extracts, chitosan, Fusanuni o.\ysporum.
Ngu6i phan bidn: GS.TS. Nguyln Vdn Tuat Ngdy nhdn bdi: 13/12/2019
Ngdy thdng qua phan bi§n: 14/01/2020 Ngdy duydt ddng: 21/01/2020
•«J - KY 1+ 2 - THANG 2/2020 163
