KHOA HOC C d N G NGHE
DAIMH GIA S U KHAC BIET CLIA CAC IVIAU GIOIVG LLIA HUYET ROMG PHLJC VLJ KHAI T H A C V A PHAT TRIEIM MGLIOIM GIEIM LLIA DAC SAlXI TAI VLIIMG B A C TRUIXIG BO
Ta Hong Unh\ Nguyen Thi Minh Nguygl^, Ph^m van Tinh^ Nguyen Thi NhM^, Nguyen BA Ngpc^, Nguyen Phi Long^, Nguyen Thi Thanh Thuy*
T6MTAT
Mue dich cua nghien cihi nay la nhSm danh gia da dang di truyen cua 08 mau gidng liia HuySt rong, bao gom 04 mSu giong thu thap tai Quang Tri. Da dang di truydn cua cac miu giong liia da duuc danh gia a mue do phan tir voi cac chi tlii SSR. Ket qua phan lich da dang di truyen ciia 8 mhi giong lua Huyet rong vai 18 cap moi SSR da thu duoc 31 alen da hinh. So alen tren mot locut dao dong tir 2- 5 alen, voi gia tri Irung binh 2,6, K^t qua phan nhom dua trdn he sd tuong dong di truyen Dice da nhom cac miu giong thanh 2 nhom chinh, Ket qua danh gia cung cho thay 04 miu giong Huyet rdng thu thap tai Quang Tri da co sir tuong ddng v^ mat di truyen va co sii khac biet di truyen so voi cac miu giong Huyet rdng thu thap tai cac dia di^m khac. Cac mau gidng liia Huy^t rong chinh la nguon gien gia tri va la nguon vat lieu quy cho cong tac chon tao gidng liia dac san oViet Nam.
Tii khda: Chi thi SSR, da dang di tniyen, lua Huyet rong.
LSATVANDE
Huyet r6ng la giong lua quy, sinh trudng va phat trien tot tai cac viing dat ngap Iti, nhifem phen nhir viing Dong Thap Muai. Gao Huyet Rong mau do nau, be doi hat gao vin con mau hong ben trong, com ngon va co vi thom, la loai gao co gia tri dinh duong cao, kha nang chong chiu khi hau va khang benh tot. Hi6n nay, dien tich canh tac liia Huyet Rong con rat it.
Theo nghidn cuu cua cac nha khoa hpc, anthocyanin duoc giii lai trong lop vo lua mau do nau la mot chat co tiem nang chdng lai cac benh ung thu, tun mach va nhieu benh khac. Neu trong mot thang danh ra khoang muoi ngay an cac loai gao mau thay gao trang bmh thuong thi co the giiip ca th^ giai dpc, ha cholesterol, giam dau nhirc gan cdt, khop xuong ngon chan tay, thai dpc td trong gan, ngira ung thu.
Do Huy^t rong la gidng lua miia truydn thdng, canh tac tir nhieu thap ky, qua trinh canh tac lau dai da lam cho gidng liia nay giam chat luong va mat ddn thi trucmg. Do vay, vi^c phuc ti-ang giong lua Huyet Rong la rat cdn thiet.
Vien Khoa hgc Nong nghiep Viel Nam Vien Di tmyen Nong nghiep ' Vi^n cay luong thuc va CSy thuc pham '^ Bp N6ng nghiep v^ PTNT
Vi6c ling dung hieu qua chi thi phan tu trong nghien cuu di truyen thuc vat da gop phan nit ngin dang ke thai gian va tang dp chinh xac ciia quy trinh san xuat gidng sieu nguyen chung tir ruong liia da thoai hoa, dong thoi giiip cho viec duy tri duac tinh da dang cua giong. Danh gia da dang di truyen nguon gien liia Huyet r6ng dia phuang dua tren kieu hinh va chi thi phan tii khong nhirng co y nghia trong viec bao ton cac mhi gidng liia dac san ma con CO dong gop quan trgng trong cdng tac chon tao gidng lua chat lupng cao.
Nghien ciru nay tap trung vao img dung chi thi phan tu di danh gia su khac biet cua 08 mhi gidng Ilia Huyet rdng thu thap tir cac dia diem khac nhau phuc vu cong tac khai thac va phat trien ngudn gen Ilia dac san Huyet Rong tai vimg B k Trung bo.
2. VAT UEU VA PHUONG PHAP NGHIBV CUU 2.1. vat li^u nghi6n cuu
- Vat Ueu thuc vat: 8 mlu gidng lua Huyet rdng (bang 1, hinh 1).
- Chi thi SSR 18 chi thi SSR cung cap boi hang IDT, My, phan bd rai rac tren 12 nhilm sac the cua he gien lua (bang 2).
- Cac hoa chat, dung cu, may moc dimg trong sinh hoc phan tir.
KHOA HOC C 6 N G NGHl
STT 1 2 3
4
5
6
7 8
Bang 1. Danh sach c^c ddng/giong Iiia su dune ti-one nehifen cihi Kyhieu
HRl HR2 HR3
HR4
HR5
HR6
HR7 HR8
T6n mhi giong Huyet rdng Thai Binh
Huyet rdng (nhap noi) Huyet rong Quang T n (dong 1)
Huyet rong Quang T n (dong 2)
Soc Trang Do
Huyet rdng Quang Tri (dong 3)
Huyet rong Quang Tri (dong 4)
Huydt rdng (nhap npi)
Nguon gde Chpn loc bang phuong phap chpn lpc ca the tir giong nhap noi
Nhap npi
Thu thap tai Trieu Phuoc Trieu Phong - Quang Tn
Thu thap tai Trieu Thuan Trieu Phong - Quang T n
Lai tao giua gidng liia Huyet rdng va giong Ilia thom STl
Thu thap tai Trieu Long, Trieu -Trifiu Phong-Quang Tri
Thu thap tai Trieu Ddng Trifiu Phong - Quang Tri
Nhap npi
' I 'r >v \0
> ^
Hinh 1. Cdc mSu gidng liia Huy^t rong su dung trong nghifen cuu B^ng 2. Danh s6ch ckc c$p mdi SSR su difflg trong nghiSn cuu
Ten mdi RMl
RM1003
RM104
RM279
RM5474
RM405
RM3663
RM276
RM20591 Trinh tu mdi
F: GCGAAAACACAATGCAAAAA R: GCGITGGTTGGACCTGAC F: GATTCTTCCTCCCCTTCGTG R- TTCCTGTCAGAACAGGGAGC F: GGAAGAGGAGAGAAAGATGTGTGTCG R TCAACAGACACACCGCCACCGC F: GCGGGAGAGGGATCTCCT R- GGCTAGGAGTTAACCTCGCG F: AAAGTGTTGGTGAGCATAGC R: TTTGTGTTTGGAGAGACGAG F: TCACACACTGACAGTCTGAC Rj AATGTGGCACGTGAGGTAAG F: CATCAACCTCCACGAACATG R: CTCGGTGGTGATCCTCCTC F: CTCAACGTIGACACCTCGTG R: TCCTCCATCGAGCAGTATCA F: TCGTCTGCGCGAATATFTAGAGAGG R ATCTGCATCGGAGTCAGCAACG
NST 1
1
1
2
3
5
5
6
6 T t o moi RM8261
RM234
RM22774
RM22257
RM23747
RM25319
RM25248
RM21
RM247
Trinh t u moi
F: GACGACTGGATGGTACGAC R: TGCTFCTCCTGCAAACAC F: ACAGTATCCAAGGCCCTGG R: CACGTGAGACAAAGACGGAG F: CGCTGGAGAAGACAAGAAACG R: TAGAATCGGATCTACGGTTCAGG F: ACGAGTGTACTAGAGATGAACTGG R; ACGACTTCCrrrACGAGTAGG F: ACCTACAGCTGAAGCCTCATACC R- GGAAGACAATCAAAGAAGCTCAGG F: AGGGTAGAGTATGTCGGTGTTTCC R CCGTGGCAGTAGCAGTAGGC F: GTGACGGCATAGGAGCGTTTATAGG Rj TA'ITGCTGTTGACCGGTATGTCG F: ACAGTATTCCGTAGGCACGG R GCTCCATGAGGGTGGTAGAG F: TAGTGCCGATCGATGTAACG R CATATGGTTTTGACAAAGCG
NST 7
7
8
8
9
10
10
11
12
Chii tiiich: NST: Nhiim sic tiie; F: Moixudi; R: M6i nguoc
NONG NGHIEP VA PHAT TRIEN NONG THON - KY 1 - THANG 11/2018
KHOA HOC C d N G NGHE
2.2. Phuong phdp nghifin ctru
2.2.1. Phuong phap phan tich bing chi thi ph^
til SSR
ADN tdng sd dupe tach chiet theo phuang phap
"NaOH extraction" cua Wang (1993). Phan ung PCR duoc tien hanh tren may Veriti 96well Tliennal cycler vai tong the tich 15 pi, gom: 5 yl ADN, 0,15 \M mdi, 0,2 mM dNTPs, IX dich dem PCR, 2,5 mM MgClj va 0,25 don vi Taq TaKaRa. Dieu kien phan ung: 95^C - 7 phiit; 35 chu ky (94"C -15 giay, 55''C - 30 giay, 72°C - 1 phiit; 72''C - 5 phut) va giu mhi b 4''C. San pham PCR duac di&n di tren gel agarose 2,5% trong dem TBE, nhuom bang EtBr va dupe soi duai den UV.
2.2.2. Phuong phip phan tich sdh'du
-h Phan tieh thdng ke: Nhirng so li&u thdng ke bao gom sd alen tren locut, tdn so allele pho bien nhat, sd alen dpc nhat, chi so PIC (Polymorphism Information Content) dupe tinh toan sii dung phan mem Excel, trong do:
- Da dang di truyen alen cua cac chi thi SSR duac danh gia thong qua he sd da hinh PIC (Botstein, 1980) va dupe tinh theo phuong trinh:
Trong do: /J,: la tan s6 xu^t hi^n ciia alen thiiy tuong ling voi moi i.
Gia tri PIC cang Ion tiic la miic dp da hinh cua locut do mdi /'khuech dai cang lan, tiic la cang nhi^u alen duac sinh ra.
•f Phan tich da dang dl truyen giua cac gidng lua:
Sii dung chuang ti-inh NTSYS-pc v. 2.1 (Rohlf, 1997) de xay dung ma tian tuong ddng di truyen vai he sd
Dice (Nei and l i , 1979) va sa do hinh cay bieu diln mdi quan he di truydn giiia cac gidng liia nghien cihi bang phuong phap phdn nhom UPGMA (Unweighted Pair-Group Method with Arithmetical averages).
3. m (HIA VA THAU LUAN
3.1. K^t qua phdn tich da hinh ADN cdc mdu gidng lua Huy^t rong bang cac chi thi phan tu SSR
Trong nghien ciiu nay, da lua chpn 18 chi thi SSR phan bd rai rac trSn cac nhi&m sdc the cua he gen Ilia de danh gia sir khac biet giua cac mdu gidng Huyet rdng nhap npi so voi cdc mdu gidng Huyet rdng thu thap tai Quang Tri (Hinh 2).
K&t qua khao sdt da hinh ADN vdi 18 chi tiii SSR thu dupe ty 1^ da hmh kha cao, 12/18 chi thi SSR cho da hinh, chiem 65,67%. Tdng sd alen thu dupe tai 12 locut cho da hinh la 31 alen, sd alen dao dpng trong khoang 2-5 alen, trung binh 2,6 alen/locut. Trong do, chi thi RMl cho sd alen cao nhat voi 5 alen (Bang 3).
Ket qua phan tich cac chi sd chinh nhu tdn sd alen phd bien nhat va chi sd da dang PIC cua cdc locut SSR cho da hinh da cho thay tan so alen phd bien nhat dao dpng trong khoang tir 40 den 87,5%, tuang img vai chi thi RMl va RM22257, voi gia tri trung binh 61,02%. Chi sd da dang PIC cua cdc locut nghifen ciiu thay ddi tir 0,22 den 0,74, voi gid tn tnmg binh la 0,49. Gia tri PIC tinng binh phan dnh mice dd da dang chung cho tat ca cac locut nghien ciiu. Dieu nay chiing to nhiing chi thi SSR dupe sir dung trong nghien ciiu nay da cho kdt qua da dang kha cao giOa cac mdu gidng liia Huyet rdng nghien cuu, cd y nghia trong phdn tich di truyen cdy liia.
Hmh 2. San ph&i PCR cua cdc mdu gidng liia Huy^t rdng v6i cdc chi thi RMl, RM21, RM276 vd RM8261 ti-en gel agarose 2,5%
Tu tiii sang phai: Thang ADN 50bp, eae miu gidng huyet rdng ky hieu tir HRl den HR8
KHOA HOC CONG NOHE
Bang 3. Cic chi tiSu v4 alen, chi s6 da dang PIC cila cic locut SSR da hinh nhan bift trto 8 mftu gidng lua TT
1 2 3 4 6 7 8 9 5 10 11 12
Chi thi SSR RMl RJVI1003 RJVI104 RM279 RIVI276 RM8261 RM234 RM22774 RIW22257 R]V125248 RM21 RM247 Tong cong Trung binh Gia tri nho nii^t Gia tri Mm nh^t
NST 1 1 1 2 6 7 7 8 8 10 11 12
So alen 5 2 2 3 3 3 2 2 2 2 3 2 31 2,6 2 5
Tan s6 alen pho bien nhat 40,00 50,00 50,00 63,64 40,00 63,64 75,00 62,50 87,50 75,00 50,00 87,50 61,02 40,00 87,50
PIC 0,74 0,50 0,50 0,51 0,66 0,51 0,38 0,47 0,22 0,38 0,63 0,22 0,49 0,22 0,74 Ghi chu: NST: Nhidm sic tiie; PfC: He soda hmh
Bang 4. MQt s6 k^t qua phdn tich da dang di truydn SSR tr^n cay liia dd dupe cdng bo TT
1 2 3 4 5 6
Tac gia
Thuy T. T. Nguyen va cs. (2012) Nguyen Thi Minh Nguyet va cs. (2013) Ngo Thi H6ng Tuoi va cs. (2014) Mengli Ma va cs. (2016) Doan Thanh Quynh va cs. (2016) Islam va cs. (2018)
Nghien ciiu nay
So gidng 41 40 46 61 42 50 8
Sd chi thi 30 27 35 78 38 50 19
Tong sd alen
192 193 68 499 106 162 31
Trung binh Sd alen
6,4 7,1 2,62 6,397 3,03 3,24 2,6
PIC 0,74 0,76 0,46 0,59 0,50 0,32 0,49 Khi so sdnh cdc ket qua thu duac trong nghien
ciiu nay vdi mpt so ket qua phdn tich da dang di truydn cua cac gidng liia voi chi thi SSR da cong bo trong va ngoai nuoe, nhan thay sd alen trung binh/
locut thu duoc trong nghien ciiu ndy thap hon cac nghien ciiu trude. Dieu ndy co the ly giai Id do cac mdu gidng trong nghien ciiu ndy ddu Id cdc mdu gidng Huyet rdng thu thdp tir cdc dia diem khdc nhau, trong khi cac nghien ciiu khac sii dung ngudn vat heu la cdc gidng lua dia phuang, gidng nhap ndi khdc nhau. He sd da hinh PIC trong nghien ciiu ndy cao hon mot sd k^t qua nghien ciiu ciia cdc tac gia Islam (2018), Ngd Thi Hdng Tuai (2014) (Bang 4).
3.2. K^t qua phan tich mdi quan h§ di truydn ciia cdc gidng liia Huy^t rong nghifin oiu
Ket qud phdn tich da dang di truyen cua cac mdu
gidng Ilia Huyet rong nghien cuu vdi 18 chi thi SSR dupe xir ly hdng phdn mem NTSYS v2.1 de nhan biet su khac biet di truyen giira cdc mdu gidng. Ket qua dupe the hidn o hinh 3 va bang 5.
Hd sd tuong ddng di truydn phan anh mdi quan he di truydn ciia cac mdu gidng liia voi nhau. Kdt qua cho thdy dp tirong ddng di truydn giira cac mdu giong Ilia nghien ciiu dao dpng tir 0,49 den 0,95 voi gid tn trung binh 0,67.
Tai he sd tuong ddng khoang 0,68, cdc mdu gidng lua Huyet rdng dd phdn thanh 2 nhdm rieng biet, nhdm 1 gdm cdc mdu gidng HRl (Huyet rdng Thdi Binh), HR2 (mdu gidng Huyet rdng nhdp npi), HR5 (Sdc Trang Do) vd HR 8 (mdu gidng Huyet rdng nhap ndO; nhdm 2 gdm 4 mdu gidng liia Huyet rong thu thap tai Quang Tn la HR3, HR4, HR6 va HR7.
NONG NGHIEP VA PHAT TRIEN NONG THON - KY 1 - THANG 11/2018
KHOA HOC CONG NGHl
N h u n g k e t q u a p h a n tich m d i q u a n M di h i i y e n giira c d c m d u g i d n g liia H u y e t r o n g t h o n g q u a m a t r a n t u o n g d o n g d i t r u y d n vd s a d o h m h c d y p h a n n h o m d i t r u y e n d a c h o t h a y c d c m d u g i d n g lua da t h e h i e n s u d a d a n g v e m a t d i t r u y d n .
Nghifen ciru n a y dd s u d u n g 4 g i d n g liia H u y e t r d n g t h u tiiap tai Q u a n g T r i , k y h i e u H R 3 , HR4, H R 6 v a H R 7 . K ^ t q u a d d n h g i a d a d a n g d i t r u y d n v o i 18 c h i t h i S S R c h o t h a y c d c g i d n g liia t h u t h a p tai Q u d n g T r i CO s u t u o n g d d n g v e m a t di t r u y d n . T r o n g 4 m d u g i d n g Ilia H u y e t r d n g t h u t h d p tai Q u a n g T r i , m d u g i d n g H R 6 vd H R 7 c d s u t u o n g d d n g di t r u y d n c a o n h d t , v d i h e s d t u o n g d o n g 0,95 ( B a n g 5 ) . K h i p h d n tich s u t u o n g d d n g di t r u y e n c u a 0 4 m d u g i d n g liia H u y d t r o n g t h u t h d p tai Q u a n g T r i d a cd s u k h d c bidt di t r u y e n s o v d i cdc m d u g i d n g liia H u y e t r d n g n h d p npi, t h u t h d p tai c d c dia d i e m k h a c .
H m h 3 . S a d6 h i n h cdy bi^u di^n mdi quan h e di truydn giiia cdc mdu g i d n g lila Huy^t r 6 n g Chii thich: He sd tuang ddng di truySn Dice dua tidn phan tich 18 chi thi SSR da the hidn mdi quan hd gida 8 miu gidng liia Huydtrdng
Jang 5. S HRl HR2 HR3 HR4 HR5 HR6 HR7 HR«
^ s o tuon HRl
1 0,85 0,63 0,56 0,68 0,55 0,60 0,63
fi d o n g c HR2
1 0,65 0,59 0,74 0,57 0,62 0,75
1 truyen f HR3
1 0,77 0-49 0,90 0,85 0,58
n O a c a c n HR4
1 0,57 0,73 0,78 0,56
[i3u gidnf HR5
1 0,56 0,60 0,68
' lua Huy HR6
1 QJ95 0,60
et r o n g n HR7
1 0,65
'hienciru H88
1 4 . KET LUAN
- K e t q u a p h a n t i c h d a d a n g di t r u y e n c u a 8 m d u g i d n g Ilia H u y e t r d n g vdi 18 c a p m d i SSR d a t h u d u p e 31 alen v d i g i d tri t r u n g b i n h 2,6 a l e n / locut. T d n sd alen p h o b i e n n a m t r o n g k h o a n g 40- 87,5%, voi g i a tri t n m g b i n h 61,02 %. C h i so d a d a n g P I C thay d d i tir 0,22- 0,74, voi gid tri tiling h i n h 0,49.
- H e s d t u o n g d d n g d i t r u y e n D i c e d u a t r e n p h a n tich 18 c h i t h i SSR d a t h e h i ^ n m d i q u a n h e giira 8 m d u g i d n g liia H u y e t r d n g . T a i h e sd t u o n g d d n g 0,68, 8 m d u g i d n g liia d a p h a n t h a n h 2 n h o m : N h d m 1 g o m c d c g i d n g H R l ( t h u t h d p tai T h d i B i n h ) , H R 2 (thu tiiap tai S d c T r d n g ) , H R 5 ( d o n g 3 t h u thdp tai Q u a n g T n ) va H R 8 ( g i d n g H u y ^ t r d n g n h d p n p i ) ; n h o m 2 g d m 3 g i d n g l u a H u y e t r d n g t h u t h a p tai Q u a n g T r i H R 3 , HR4, H R 6 vd g i d n g l u a H u y e t r d n g n h d p n o i H R 7 .
- K e t q u a d a n h g i a dd c h o t h a y cdc g i d n g liia H u y d t r d n g t h u t h d p tai Q u a n g T r i c d s u tirong d d n g
v e m a t di t r u y e n v a t h 6 h i e n s u k h a c h i e t di t r u y e n s o vdi c a c m d u g i d n g H u y e t r d n g t h u t h a p tai c a c d i a d i ^ m k h a c .
L C f l c A M O f N
Cdng trinh nghien am niy li kdt qui thudc Nhidm vu quy gien cap Qudc gia "Khai thae va phat trien ngudn gien liia dae sin Huydt Rdng tai vung Bic Tnmg bd " thude Chuong trinh khai thac vi phat trien ngudn gien do Bd Khoa hpe Cdng nghd cap lanh phi
TAI UEU THAM KHAU
1. N g d T h i H o n g T u o i , P h a m V a n C u d n g , N g u y e n Vdn H o a n (2014). P h d n tich d a d a n g di t r u y e n c u a c a c m a u g i d n g liia c a m b a n g c h i t h i SSR.
T a p c h i K h o a h p c va P h d t t n e n 2014, t d p 12, s d 4:
485-494
KHOA HOC C 6 N G NGHE
2. Nguyen Thi Minh Nguyet, Khuat Hiiu Province. Advances in Computer Science Research, Trung, Nguyen Thi Nhai, Nguyen Thiiy Diep, Kieu volume 63:154-158.
Thi Dung, Nguyen Thi Thao, Nguydn Thi Thanh ,- „ . , , ^ , „ „ , . , T ^ V O „ri,.,l TI,,',,, raniQi a- X. •• i J T • ' , 5. RohH F., 1997. NTSYS-pc: numencal
rhuy (2013). Danh gia da dang di truyen tap doan ^ ^^ multivariate analysis system, 2.1 edn.
lua ch,u han cua VietNam bang chi thi phan hi SSR Department of Ecology and Evolution, State Tap chi Nong nghiep va PTNT, 1, tr. 12-18. University of NY, Stony Brook.
3. M. Z. Islam , M. Khalequzzaman, M. F. R. K. g - „ , j ^ ^ Nguyet M. T N., Long H. H., Pnnce M. A. Siddique, E. S. M. H. Rashid, M. S. U. Nanito R and Kenichi T (2012). Genetic Diversity in Ahmed, B. R. Pittendrigh, M. P. AU, 2018 Diversity Vietnamese Upland Rice Germplasm Revealed by and populabon steucture of red rice germplasm in gSR Markers. J. Fac. Agr., 57 (2), tr. 383- 391.
Bangladesh. PLoS ONE 13(5): e0196096.
https://doi.org/10.1371/ioumal.pone.0196096 ^- "^^^ " ' ^ « ' ™<^ Sutler A. J (1993). A simple method of preparing plant samples for PCR.
4. Mengh Ma, Yanhong Uu, Hengling Meng NucleicAcids Res., 21(17): 4153-4154.
and Bingyue Lu, 2016. Genetic Diversity Analysis of
Red Rice from Hani's Terraced Fields in Yunnan ^- ^ ™ ^ ^ ^ http://www.gramene.org/.
EVALUATING THE DIFFERENCES OF RED RICE SAMPLES FORTHE EXPLOITATION AND DEVELOPMENT OF INDIGENOUS RICE GENETIC RESOURCES IN THE CENTRAL NORTH
VIETNAM
Ta Hong Linh, Nguyen TTii Minh Nguyet, Pham Van Tinh, Nguyen Tlii Nhai, Nguyen Ba Ngoc, Nguyen Phi Long, Nguyen Thi TTianh TTiuy
Summary
The purpose of this study was to evaluate the genetic diversity within 08 Huyet Rong rice accessions, including 04 accessions coUedted in Quang Tri province. Genetic diversity of the nee accessions was evaluated at the DNA level. Eight accessions were surveyed with 18 simple sequence repeat (SSR) markers reveabng the genetic relationship among the varieties. A total of 31 polymorphic bands were detected. The number of alleles per locus ranged from 2 to 5, with an average of 2.6. Cluster analysis based on Dice's sinulanty coefficients usuig UPGMA grouped the nee accessions into two major groups. The results showed that the 04 accessions coUedted in Quang Tn province showed genetic similanty among them and the genetic difference compared to accessions collected at other sites. The Huyet Rong rice accessions presents a valuable gene source and sufficient genetic background for specialty rice breeding in Vietnam.
Keywords. Genetic diversity, Huyet Rong rice, SSR marker
Ngudri phan bi^n: PGS.TS. La Tuan Nghia Ng^y nhan b ^ : 3/8/2018
Ngay thong qua phan bi0n: 5/9/2018 Ng^y duyet dang: 12/9/2018
NONG NGHIEP VA PHAT TRIEN NONG THON - KY 1 - THANG 11/2018
