Nghien CLFU - Ky thuat
Cae nghien cuu ve co e h l chong viem ciia tam phdng tren t h i gidi cho t h i y u-c e h l phespholipase A2, ue che C 0 X 2 , giam TNFa va giam NO tai md viem la nhu-ng dieh tae dung quan trpng eiia djeh ehilt t i m phdng. Chua cd nghien eu-u nao chi rd thanh p h i n boat c h i t trong eay t i m phdng ed tac dung ehdng viem.
Ket luan
- Djeh chilt nude va djeh chilt edn eay t i m phdng (lilu 10 g/kg) ehua the hien heat tinh khang k h u i n tren cac ehiing vi k h u i n £ coliva Samoneila sp..
- Ca djch chiet n u d c va djch e h i l t con eay t i m phdng (lilu 10 g/kg) d i u t h i hien tac dung chdng viem c l p qua md hinh gay phii chan chudt bang carrageenan va gay tran djch mang bung bang hen hgp carrageenan + formaldehyd.
- Chi ed djch c h i l t n u d e t i m phdng 10 g/kg t h i hien tac dung chong viem man thdng qua lam giam trpng lugng khdi u tren md hinh gay u hat thge nghiem bang d y amiant d u d i da.
Ca djch ehilt n u d e va dich c h i l t cdn t i m phdng (lilu 10 g/kg) d i u khdng anh h u d n g d i n trpng lugng t u y i n uc sau khi cho ehudt udng thudc lien tuc treng 3 ngay.
T a i lieu t h a m k h a o
1. B5 Huy Bich, Dang Quang Trung, NguySn Thirgng Dong (2006), Cay thuoc va ddng vat lam thudc a Viet Nam, tap 2, Nha xuat ban Khoa hpc ky thuat, trang 794-795.
2. Le Thj DiSm Hong, Nguyen Xuan Thing (2003),
"Gop phan tim hieu tac dung chong viem cua hoa cay {Ngay nhan bai: 20/03/2016
kim ngan (/.on/cerayapon/ca Thunb.)", Tap chi Dugc hoc s64/2003, trang 13-15.
3. H. Gerhard Vogel (2002), "Drug discovery and evaluation, pharmacological assays", Springer-Veriag Beriin Haldelberg, pp. 725-771.
4. Jigna Parekh, Sumltra V. Chanda (2006), "In vito antimicrobial activity and phytochemlcal analysis of some Indian medicinal p\ani\TurkJ. Biol., 31 (3), pp 53-58.
5. M. S. Sheeba and V. V. Asha (2006), "Effect of Cardiospermum halicacabum L. on ethanol-induced gastric ulcer in rats", J. of Ethnopharmacology, volume 106, Issue 1, pp. 105-110.
6. Sadique, J. Chandra, T. Thenmozhi, V. Elango (2007), "Biochemical models of action of Cassia occidentalis and Cardiospermum halicacabum in inflammation". International Bibliographic information Dietary Supplements, 19(2), pp. 201-212.
7. 8. M. Jachak, C. Selvam, A. 8. Rivastava, V.
Ahuja (2007), "Evaluation of anti-inflammatory activity and Identification of bioactive compounds from Vitex negundo L., Cardiospermum halicacabum L. and Tridax procumbens L.", Theme Connect, 73.
8. The European agency for the evaluation of medicinal products (1990), "Committee for veterinary medicinal products, Cardiospermum halicacabum L.
report". Veterinary Medicines Evaluation Unit, 664, 99.
9. Venkatesh Babu K. C. and Krishnak U. M.
(2006), "Cardiospermum halicacabum L. suppresses the production of TNF-alpha and Nitric oxide by human peripheral blood mononuclear calls", African J. of Biomedical Research, Vol.9, pp. 95-99.
10. Z. Phytother (2005), "Treatment of eczema with Cardiospermum halicacabum L., Cardiospermum ointment and ointment based on a part-place comparison controlled trial", Saibbe and Sallbengrundlage in Halbseitenverglerich, 5, pp. 263-266.
• Ngay duyet dang: 25/04/2016) r
Nghien cmi tac dung giam dau va chong viem cua cay goi hac
Nguyin Thi Phu-ffng'*, Nguyin Thuy Du-ong^ Dau Thi Giang^
Nguyin Minh Khoi', Phu-ong Thien Thu'o'ng'
' Vien Duac lieu - Truang Dai hoc Duac Hd Npi ' E-mail: phuong? 6. hpttc@gmail. cam
Summary
The analgesic and anti-inflammatory activities ofLeea mbra Blume (Leeaceae) extracts were investigated in mice and rats. Acute and chronic anti-inflammatory activity was estimated by the model of earrageenan- induced paw edema and cotton pellets in rats. Analgesic effect was tested in mice by hot plate and writhing test to distinguish the central and peripheral effects, respectively In writhing tests, effects of the ethanol extracts of root and leaf were significant at doses of 100; 200 and 400 mg/kg. By the model of canrageenan-indueed paw edema, both root nad leaf extracts showed a trend to reduce the edema at doses of 100 and 200 mg/kg
34 TAP CHi Dl/OC HOC - 5/2016 (SO 481 NAM 56)
• Nghien
CIFU- Ky thuat
in comparison with the control Also, by the cotton pellet model tiie leaf extracts reduced granuloma weight at doses of 100 and 200 mg/kg, but so did the root ones only at the dose of 200 mg/kg. These findings may provide scientific validation to the folk medicine use ofL rubra for treatment of inflammation and relief of pains in Vietnam.
Keywords: Leea rubra Blume (Leeaceae), anti-inflammatory, antianaigesic, extract Dat van de
Cay gdi hae ed ten khea hpe la Leea rubra Blume ex Spreng., hp gdi hac (Leeaceae). Cay du-gc tim thiy d nhilu qude gia nhu- An Dp, Bangladesh, Thai Lan.... 6l Viet Nam, gdi hae phan bd rdng tu Hda Binh, VTnh Phue, Hul vae tdi Bac Lac, Ba Rja-Vung Tau... V l cdng dung, r l cu gdi hac duge diing chu-a benh dau nhu-c xuong khdp, te thap, chu-a sung tiy, sung diu gdi do pheng thip '^' ^K Tuy nhien, khdng chi d Viet Nam ma ca tren t h i gidi, eae edng trinh nghien cu-u v l thanh phin hda hpc, tae dung sinh hpe ciia leal nay edn tuong ddi ban ehl. Treng bai bao nay, chung tdi cdng bd kit qua nghien ciru tren ddng vat thge nghiem v l tae dung ehdng viem, giam dau ciia eao ehilt r l va la eua eay gdi hae, nham gdp phin sang td cdng dung ciia dugc lieu dugc sir dung trcng dan gian.
Nguyen lieu va phu'O'ng phap nghien ciru Nguyen lieu
Dugc lieu dung treng nghien ciru la la, r l eiia eay gdi hac dugc thu hai tai tinh Bic Giang nam 2012. Miu dugc giam djnh ten khoa hpc bdi Tiin sy Dd Thi Xuyin, Bd mdn Thge vat, Khoa Sinh hpc, Trudng Dai hpe Khoa hpc Tg nhien - Dai hpc Quic gia Ha Npi. Miu dung che nghien ciru la cao khd ethanol 96 %. Cae la (GHL) ed hieu suit chilt 6,5%, dp i m 4,6%; eao r i (GHR) cd hieu suit ehilt 3,1%, dp I m 1,5%).
Dong vat thi nghiem
Chudt nhit tring chiing Swiss, gidng dgc, can nang tu-18-22 g, khde manh do Vien Ve sinh djch t l Trung uong cung d p . Chudt cdng trang ehiing Wistar, gidng dgc, 8-10 tuin tuoi, can nang tu-140- 160 g, khde manh do Hpe vien Quan y cung cip.
Ddng vat duge nudi on djnh vdi dieu kien phdng thi nghiem Bd mdn Dugc Igc, Trudng Dai hpc Dugc Ha Ndi bang thirc an tieu chuan, udng nudc tg de it nhit 5 ngay tnj-dc khi thge hien nghien ciru.
Phu-o-ng phap nghien ciru
Danh gia tac dung giam dau frung irong fren mo hinh mam nong
Tac dung giam dau trung uong duge danh gia thdng qua md hinh mam ndng ^ "'. Chudt nhat trang du-ge ehia thanh cac Id: Id chirng udng dung mdi. Id ddi chilu udng codein lilu 60 mg/kg va
cac Id thir udng cao goi hac (GHR va GHL) vdi ba mirc lilu 100, 200, 400 mg/kg, Hen tue trong 5 ngay. Sir dung Hot plate LE 7406 (PanLab) d l xac djnh thdi gian phan ipng dau cua tipng ehudt lue binh thudng va sau khi dung thudc lin eudi 90 phut.
Danh gia tac dung giam dau ngoai vi tren mo hinh gay dau quan bang acid acetic
Sit dung md hinh bay dau quan d l danh gia tae dung giam dau ngoai vi P- "l Chudt nhat trang duge chia thanh cae Id: Id chu-ng udng dung mdi.
Id ddi chilu udng indomethacin lilu 10 mg/kg va cae Id thir uong cao gdi hac (GHR va GHL) vdi ba mipc lilu 100, 200, 400 mg/kg, lien tue treng 5 ngay. Sau khi udng thudc va miu thir lin cudi 1 gid, tiem mang bung dung dich acid acetic 1% va dim s l eon quan dau ciia tipng chudt trong tirng 10 phiit mdt che den phut thip 30.
Danh gia tac dung chong viem cap tren mo hinh gay phu ban chan chugt bang carrageenan
Sir dung md hinh gay phu ban chan ehudt bing carrageenan P' ^i Chudt edng trang dgc du-gc ehia thanh cac Id: Id chirng udng dung mdi. Id ddi chilu udng indomethacin lilu 10 mg/kg va cac Id thip udng eae gdi hac (GHR va GHL) d hai mipc lilu 100 va 200 mg/kg, lien tuc trong 5 ngay. Sau khi uong thude va miu thip lin cudi cung, chupt dugc tiem 0,1 ml carrageenan (Sigma Aldrich) 1% trong nude mudi sinh ly vae gan ban chan sau phai.
Dung may de dp phu Plethysmometer LE 7500 (Letica Scientific Instruments) d l de t h i tieh ban chan sau ben phai trudc khi gay viem va tai eae thdi dilm 1, 3, 5 va 7 gid sau khi gay viem. Xae djnh mirc dp phu ban chan phai cua tirng chudt AV (%) va phin tram (yc c h i phii cua eae Id thir so vdi Id chirng I %.
Danh gia tac dung chong viem man tren mo hinh gay u hat thirc nghiem bang vien bong
Tae dung chcng viem man duge tiin hanh thee phuong phap gay u hat thge nghiem bing vien bdng '' "l Chudt eong tring duge c i y vien bdng (khdi lugng ehinh xac kheang 20 mg) vae viing da dudi lung va dugc chia thanh cac Id: Id chirng udng dung mdi, Id ddi chilu uong prednisolon lilu 5 mg/kg va eae Id thir udng cao gdi hae (GHR va GHL) d hai muc lilu 100 va 200 mg/kg. Sau 7 ngay dung thudc va mlu thir.
TAP CHI DU'OC HOC - 5/2016 (SO 481 NAM 56) 35
Nghien CCFU - Ky thuat
bde tach u hat dudi da lung eiia ehudt. Xac djnh khdi lugng u hat tuoi va u hat khd cua tu-ng chudt. Tinh ty le % dp giam khii lugng u hat eiia Id thir se vdi Id chu-ng P"'.
Phirong phap xir ly so lieu thong ke Vdl eae sd lieu thude phan phdi chuin, kit qua dugc bilu diln dudi dang M ± SE (M: gia trj trung binh tung Id, SE: sai so ehuan). So sanh gia trj trung binh giu-a eae Id bang one-way ANOVA, diing hau kilm d l se sanh gia trj trung binh ciia cac Id thir so vdi Id chipng. Vdi eae sd lieu khdng thude phan phii ehuan, kit qua duge trinh bay dudi dang trung vj (tir phan vj), dung thuat toan Kruskal Wallis d l so sanh giu-a cac Id va Mann-Whitney U test d l so sanh giu-a Id thir vdi Id chirng. Su khae biet dugc coi la ed y nghTa thing ke khi p < 0,05.
Ket qua nghien cu'u
Tac dung giam dau trung iro-ng cua cao GHL va GHR tren mo hinh mam ndng
Bang 2: Anh huang ciia cao GHLva GHR
Kit qua the hien a bang 1
Bang 1: Anh huang ciia cao GHL va GHR din thdi gian phan img dau eiia ehudt nhit tring
Lo va lieu dung Chimq
Codein 60 mq/kq GHL 100 mg/kg GHL200mq/kg GHL 400 mq/kq GHR 100 mq/kq GHR200mq/kg GHR 400 mg/kg
n 18 18 16 18 18 18 18 18
Thai gian phan irng dau (glay) 17,38 + 1,46
27,94 ±1,97**
16,03 ±1,03 17,74 ±1,38 21,36 ±1,73 18,62 ±1,32 21,31 ±2,46 21,73 ±1,68
** p < 0,01 khi so sanh vailo chimg Thdi gian phan u-ng vdi nhiet dp cua chudt uIng cao GHL va GHR d t i t ea eae mu-c lieu thir tai thdi diem sau khi uong thudc 90 phut khdng khac biet so vdi Id chipng. Trong khi dd. Id diing codein lieu 60 mg/kg cd tac dung keo dai thdi gian phan irng vdi nhiet dp ciia chudt so vdi Id chu-ng (p < 0,01).
Tac dung giam dau ngoai vl cua cao GHL va GHR tren mo hinh gay dau quan bang acid acetic
Kit qua t h i hien d bang 2.
din so can quan dau ciia chudt nhit tring
Ld va lieu dung Chirng Indomethacin 10 mg/kg
GHL 100 mg/kg GHL 200 mg/kg GHL 400 mg/kg GHR 100 mg/kg GHR 200 mg/kg GHR 400 mg/kg
n 18 20 18 18 18 20 18 20
0-10 phut 27 (21-39)
14 (11-19)**
17 (10-26)**
17 (14-22)"
20 (10-34)*
19 (15-25)"
23 (14-28)*
18 (14-25)"
So con dau quan 11-20 phut
28 (24-35)
19 (14-27)"
27 (15-34)
25 (19-29)*
23 (15-39)
23 (19-29)*
23 (18-29)*
18 (14-25)"
21-30 phiit 15 (12-21)
10 (8-16)*
16 (11-24)
12 (5-15)*
15 (8-25)
13 (9-19)
13 (7-19)
12 (7-18)
72 (55-96)
38 (34-65)"
60 (35-84)
53 (39-64)**
56 (39-86)*
54 (45-74)*
59 (44-65)*
55 (37-60)"
",p<0,01, *,p<0,05 khi so sanh voi 16 chimg Cao GHL lieu 100 va 400 mg/kg cd tac dung
lam giam s l con quan dau eiia ehudt so vdi Id chirng trong kheang 10 phut diu (tuong u-ng p <
0,05 va p < 0,01); lilu 200 mg/kg cd tac dung nay trong tpan bd thdi gian nghien cuu. Tinh tong qua trinh theo ddi 30 phiit, cae GHL lieu 200 va 400 mg/kg thi hien tae dung lam giam s l eon quan dau so vdi chirng.
Cae GHR lilu 100 mg/kg ed tac dung lam giam sd eon quan dau eiia chudt sc vdl Id chu-ng trcng khoang thdi gian 10 phut diu (p < 0,01) va
10 phut tilp thee (p < 0,05); eung trong 20 phiit nay, tac dung giam sd eon dau quan eung t h i hien rd vdi lilu 200 mg/kg (p < 0,05) va lilu 400 mg/kg (p < 0,01). Tinh thee tdng so eon quan dau trong toan bd qua trinh thep ddi 30 phut, cao GHR ca 3 mu-c lilu thir deu t h i hien tae dung lam giam s l eon quan dau so vdi chirng.
Indomethacin lilu 10 mg/kg the hien tac dung giam dau tdt tren md hinh nay, lam giam so con quan dau ciia ehudt se vdi Id chirng trong toan bp khoang thdi gian 30 phut thir nghiem.
36 TAP CHI DlTOC HOC - 5/2016 (SO 481 NAM 56)
Nghien
CIFU- Ky thuat
Tac dung chong viem clp ciJa cao GHL va GHR tren mo hinh gay phii ban chan chugt
bang carrageenan
Kit qua the hien d bang 3.
Bang 3; Anh hucmg ciia cao GHL va GHR len mirc do phii chan ehudt (%) theo thdi gian
Lo va lieu diing Chiing Indomethacin 10 mg/kg
GHL 100 mg/kg
GHL 200 mg/kg
GHR 100 mg/kg
GHR 200 mg/kg
n 8 8 10 10 10 10
Thong sd - AV(%) AV(%) 1%
AV (%) 1%
AV (%) 1%
AV (%) 1%
AV (%) 1%
Sau 1 gio- 25,44±1,16 13,36 ± 1 , 8 5 "
47,5 16,76 ± 1 , 5 6 "
34,1 1418 ± 2 , 2 2 "
44,3 17,06 ± 1 , 7 5 "
32,9 19,19 ±1,99*
24,6
Thcfidiem Sau 3 gia 36,98 ±2,11 10,1 + 1,69"
72,7 24,39 ± 3,50*
34,1 20,37 ± 2,66"
44,9 25,8 ±3,16*
30,2 22,34 ± 5 , 0 1 "
39,6
Sau 5 gia 30,95 ± 2,41 8,23 ± 2 , 1 5 "
73,4 20,95 ± 4,87
32,3 15,27 ± 2 , 2 0 "
50,7 21,73 ±3,33
29,8 22,34 ± 4 1 0
27,8
Sau 7 gia 25,03 ± 2,52 14,57 ± 3,93*
41,8 15,96 ±2,31
36,2 21,73 ±3,00
13,2 22,99 ± 2,69
82 25,54 ± 3,55
- ', p < 0,05 va ", p < 0,01 khi so sanh voi Id chimg
Cao GHL lilu 100 mg/kg cd tae dung ire chi phu ban chan ehudt tai eae thdi dilm 1 gid, 3 gid sau khi gay viem, ty le ipc ehl phu se vdl chipng la 34,1% (p < 0,01 va p < 0,05). Lieu 200 mg/kg cd tac dung ire c h i phu ban chan ehudt tai ba thdi dilm 1 gid, 3 gid va 5 gid sau khi gay viem, ty le uc ehl phil se vdi chu-ng iln lugt la 44,3% , 44,9%
va50,7%> vdi (p< 0,01).
Cao GHR lilu 100 mg/kg cd tac dung ire ehl phil ban chan chudt tai cae thdi diem 1 gid va 3 gid sau khi gay viem, ty le ire ehl so vdi chung tuong ung la 32,9% (p < 0,01) va 30,2% (p < 0,05). Lilu 200 mg/kg cd tac dung ipc ehl phii ban chan chudt tai cac thdi dilm 1 gid va 3 gid sau khi gay viem, ty- le LPC ehl so vdi chung iln lupl la 24,6% (p < 0,05) va 39,6% (p< 0,01).
Indpmethaein lilu 10 mg/kg t h i hien tae dung ching viem tot, tai thdi dilm sau 1 gid - 3 gid - 5 gid, ty le LPC chi iln lugt la 47,5% - 72,7% - 73,4%
(p < 0,01) va sau 7 gid la 41,8% (p < 0,05).
Tac dung chong viem man cua cao GHL va GHR tren md hinh gay u hat thiFC nghiem bang bong
Kit qua t h i hien d bang 4.
Bang 4: Anh huang cua cao GHL va GHR len khdi lugng u hat tren chudt cdng tring
Lo va lieu diing Chimg
Prednisolon 5 mg/kg GHL 100 mg/kg GHL 200 mg/kg GHR 100 mg/kg GHR 200 mg/kg
* p < 0,05 va n 8 8 8 8 8 8
".P
Khoi lirgng u hat tuKri (mg) 531,91 ±54,16 278,66 + 24,95 "
372,94 ± 3 5 2 1 * 405,33 + 25,93*
574,25 ±57,59 384,10 ±39,67*
Khoi lirang u hat kho (mg)
79,26 ± 9,56 27,51 ± 4 , 5 3 "
47,93 + 6 1 8 "
59,69 ± 3,50*
71,76 ±7,29 57,24 ±7,18*
<0,01 khi so sanh vai Id chimg
TAP CHI DLTOC HOC - 5/2016 (SO 481 NAM 56)
So vdi chipng, eae GHL lilu 100 mg/kg lam giam khdi lugng u hat tuoi va khd lin lugt la 29,9% (p < 0,05) va 39,5% (p < 0,01), trong khi dd lieu 200 mg/kg lam giam tuong irng la 23,8% va 24,7%, (p < 0,05). Cao GHR lilu 100 mg/kg khdng t h i hien tac dung chdng viem man nhu-ng lieu 200 mg/kg lam giam ea khoi lugng u hat tuoi va u hat khd so vdi chirng, ty le giam ciing d mirc 27,8% (p
< 0,05). Tren md hinh nay, prednisolon lilu 5 mg/
kg t h i hien tae dung chdng viem man rd ret, lam giam dang k l khdi lugng u hat tuoi va u hat khd se vdi Id chirng (p < 0,01).
Ban luan
Thee YHCT, r l gdi hac ed vj ding ngpt, tinh mat, cd tac dung tieu sung, thdng huyltP'. Hien nay, gdi hae dugc sir dung kha pho biin nhung eae edng bd khea hpe ve tae dung sinh hpc eiia Icai nay edn ban ehl. Bd phan dung d l dllu trj viem dau khdp treng y hpc eo truyin ciia gii hac dugc ghi treng cac tai lieu la r l eay. Tuy nhien, nhdm nghien ciru da tiin hanh danh gia tac dung dugc ly lien quan din tinh vj ciia leai nay tren ca bd phan thudng diing la r l va bd phan d l thu hai ciia gdi hae la la.
Thilt k l mipc lieu thir tac dung sinh hpc eua gdi hac trong nghien cuu nay duge dga tren tri thire dan gian. Treng cac bai thude YHCT, r l gdi hae dugc dung chu-a benh dau nhire xuong khdp, te thip, sung diu gdi de phong thip vdi lilu tip 12 g va CO t h i len din 30 g mdi ngay phdi hgp vdi cac vj thude khae " ^i. Tu mipc lilu 30 g tham khae dugc kit hgp vdi bleu suit chilt eao re, qui ddi sang lilu tren ehudt nhit trang tuong duong 200 mg/kg. De dd, cao gdi hgc dugc ra seat tac dung giam dau tren chupt nhat tring (ddng vat nhd) vdi ba mire
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