TAP CHI HOA HOC
DOL W. 15625/0866-7144.2015-0087
T.53(1) 50-55 THANG 2 NAM 2015
THU NHAN XENLULOZa TIJ BA MIA CHO S A N X U A T ETANOL SINH HOC THEO PHl/ONG PHAP XCT LY VOI AXIT AXETIC
Le Quang Diln'*, Pham Tuan Anh', To Kim Anh', Nguyin Thi Minh Nguyet' 'Tnrang Dgi hgc Bdch khoa Hd Ngi
^Trudng Dai hgc Ldm nghiep Din Tda sogn 12-9-2014; Chip nhgn ding 12-02-2015
Abstract
Sugarcane bagasse contains more than 40 % of cellulose; this material presents a potential feedstock for bioethanol production. In this study, the two-stage treatment of bagasse was used for preparation of cellulosic pulp. Firstly, sugarcane bagasse was treated by mixture of acetic acid and hydrochloric acid (99/1 v/v) in conditions of liquor- lobagasse ratio (10:1) I/kg, temperature (80-90 °C) and 60-90 mins of cooking. Secondary, preUeated bagasse was submerged in sodium hydroxide solution (0.75-1.0 % w/w), at temparature of 80-90 "C, for 30 mins. The yield of cellulosic pulp, which presents 80-85 % purity, was 51-53% of over dry bagasse. Enzymatic saccharification of pretreated bagasse by Novozymes commercial enzyme Cellic®CTec2 with dosage of 45 FPU/g of pretreatred bagasse achieved the reduce sugar yield an average 36.2-38.0 % of over dry bagasse. Fermentation of sugars has been succesflil.
Study on chemical recovery showed that at laboratory conditions more than 75 % of consumed acetic acid and hydrochloric acid can be recovered by vacuum distillation and can be reused for obtaining of cellulose.
Keywords. Bagasse, acetic acid treatment, enzymatic saccharification, bioethanol, reduce sugars
Nhiing nam gin diy, cic dang sinh khdi chiia xo sgi (lignoxenlulozo) la phe thai, phe phu phim cdng, ndng, lim nghiep, di thu but su quan tim ciia nhilu nghien ciiu trong va ngoai nude, bdi ngoai gii tri la ngudn nguyen lieu tai sinh dl chi bien, viec tin dung triet de ciy cdng nghiep, ndng nghiep se mang lai nhung lgi ich ldn ve bao ve mdi trudng va ning cao gia tri sin xuat.
Mgt trong sd nhiing dgng phe phu phim ciy cdng nghi?p ngan ngay, cd tiem ning frir lugng ldn d nude ta, la ba mia, nhung hien nay mdi chi cd mgt vai phuong thiic tan dung, nhu lam chit ddt Id hoi, phin bdn vi sinh, sin xuat bgt giiy.
Vol xu hudng tang cudng sir dung va nghien cuu cdng ngh? sin xuit nhien lieu sinh hgc the he hai tir ngudn sinh khdi lignoxenlulozo, ba mia ciing da dugc nghien cuu tan dyng theo nhimg phuang phap khic nhau [5-10, 12], frong dd cac nghien ciiu vdi cac quy trinh cdng ngh? dugc de xuit ehii yeu tip trung vao cdng dogn tiln xir ly cho qua frinh dudng hda xenlulozo, vdn li cdng doan quan frgng ciia qui trinh sin xuit etanol tir vat lieu lignoxenlulozo [11].
Trong nude mdi chi cd mgt sd nghien cim dugc
cdng bd, nhu sii dung phi thii gd [1, 2] hay rom ra [3], chua cd nghien ciiu nao ve vin de nay. CJ quy md cdng nghiep, xenlulozo dugc thu nhgn tir ba mia theo cac phuang phap truyin thdng la nau kilm hay niu sunfit [14].Tuy nhien cac phuong phap nay khdng phii hgp lam vdi cac dgng nguyen lieu nhu ba mfa va de san xuat d quy md vira v i nhd, bdi khd cd the dap ling dugc nhihig yeu ciu v l cdng ngh?, nhu ngan ngira dugc khi cd miii sinh ra trong qua trinh sin xuat, xenlulozo cd ham lugng cao va it bi phan huy, dilu kien nhiet do v i ip suit khdng cao (chi khoang 100°C), tiet kiem dugc ning lugng va khdng ddi hdi cao vl thiit bi chuy8n dung, ddng thdi van hanh it phiic tap, cd thi thu hdi hda chit va tai sir dyng, ddng thdi quy trinh cdng nghe cho phep san xuat nhieu san pham phy song song vdi san phim chinh la xenlulozo [15].
Thu nhan xenlulozo va vgt lieu xo sgi tuong duong, theo phuong phap niu sir dyng dung djch cac hgp chit hiru co lam tac nhan tach loai lignin, la mpt trong nhiing phuong phap triln vgng, dap irng dugc cic yeu cau neu tren, da dugc triln khai nghien cim ung dyng fri lau [4], trong do axit axetic la tie nhan tich logi lignin hieu qua ddi vdi nguyen li?u than thao ngin ngay [3, 4, 13]. Vin dk cdn lgi chi la cin nghien ciiu ap dyng cho timg loai nguyfin lieu cy
TCHH, T. 53(1), 2015
the, de cd duoc quy frinh cdng nghe thich hgp, kha thi.
Trong nghien cihi nay, chiing tdi frinh bay ket qua nghien ciiu thu nhin xenlulozo tii ba mia, theo phuong phap nau sii dyng axlt axetic cd bd sung axit clohydric, frong mdt so nghien ciiu sii dung bi mfa cho san xuat etanol sinh hgc.
2.VAT LIEU v A PHU'ONG P H A P
Ba mia khd gid dugc liy mau tgi Cdng ty cd phin dudng Lam Son, Thanh Hda, dugc nghiin bing may nghiin biia vdi sang Id 7,0 mm, sau do dugc sang chgn vdi sing 0,5 mm dl tach bd phin miin vun. Nguyen lieu thu dugc de deu am va sir dyng cho nghien ciiu.
Thanh phin hda hgc co ban cua nguyen lieu dugc phin tfch theo cic phuong phip tieu chuin hda TAPPI.Thu nhgn xenlulozo dugc tien hanh theo quy trinh nhilu cdng dogn vdi so dd sau:
nghien nho
Xii ly bSiig -\ Thu hoi a axit axetic "^ -'"-
Trich ly Idem
B$t / - s i y xenlulozff *"
Riia, vat »- Riia.
3 1
uucfc •" tmng hoa kiem Xu ly ba mi'a (mdi me 20 g) dugc tien hanh d dilu ki?n ap suat thudng. Thanh phin djch niu, ti djch (ran:ldng), nhiet dg va thdi gian xOr ly, dugc dilu chinh tuy theo muc tieu ciia tung thyc nghiem.
Hl?u suit bdt dugc xac djnh bing phuang phap sij; khd (TAPPIT207 cm-99). Ham lugng cac thanh phan hda hgc co ban cua ba mia dugc xac dinh theo cac phuang phip tieu chuin hda: xenlulozo (TAPPI TI7); lignin (TAPPI T222); pentozan (TAPPI T223); cac chit tan frong nude ndng (TAPPI T207) va dg fro (TAPPI T211).
Dudng hda bgt ba mfa sau tien xu ly bang enzyme Celtic® CTec2 dugc tien hanh vdi miic su dyng 45 FPU/g bdt khd gid. Ham lugng dudng khu dugc xac dinh theo phuong phap do mat do quang hgc bang miy do quang Optima-sp 300.
3. KET QUA VA B A N LUAN
Die thii ciu tao cua ba mia la vat lieu xo sgi cd kich thude khdng Idn, khdng ddng deu, xdp, de mim, dl phan hiiy sinh hgc ngay trong dilu kien tu nhien khi ton trO. De cd the chi biin dgt hieu qua, can nghien so bd. Da xac dinh dugc, phan nguyen li^u phii hgp cho chi bien (tren sang 0,5 mm, tuong duong vdi ludi 50 mesh) chiim 87 %, cdn lai la mim vyn, chiia ca cat san, tap chit bin, frong thuc t l sin xuit cd the tach bang cic thiet bj sang chgn phd bien
Li Quang Diin vd cgng stf cd ning suat cao. Thanh phan hda hgc co ban ciia nguyen lieu ba mia, sir dyng cho nghien ciru da dugc xac dinh bao gdm: xenlulozo (44,02 %); lignin (25,49 %); pentozan (18,70 %); cac chit tan frong nude ndng (5,01 %); cac chat tan trong dung dich NaOH 1% (39,84 %) va do tro (5,19 %). Him lugng xo sgi chiem > 60 %, trong dd xenlulozo chiim > 40
%, la nguyen lieu phii hgp cho san xuat etanol.
Nhu da biet ve mgt hda hgc, bin chit ciia qua trinh thu nhin xenlulozo tir sinh khdi lignoxenlulozo la tach loai lignin, sii dyng cic tic nhin khac nhau.
Uu diem ciia sii dyng axlt axetic hay axit focmic de tach loai lignin la cd the tien hinh qua frinh d nhiet dg thip, cac axit de bay hoi cd the thu hdi vi tai sii dung, xenlulozo thu dugc cd do ben co hgc cao, do ft bi phan hiiy hon so vdi d nhiet do cao nhu trong qua trinh niu sunfit hay niu sunfat [4, 14].
Nhiem vu ciia nghien ciiu la xac lap dugc cac gii tri thich hgp, eiia cac thdng sd cdng nghe qua trinh niu n ^ y e n lieu hi mia bing dung dich axit axetic, bao gdm mirc sii dyng hda chit, nhiet do va thdi gian, ti djch, de thu dugc bgt xenlulozo cd ham lugng xenlulozo > 80 %. Qua mgt sd nghien ciiu so bO, da xae dinh dugc, diy la miic chit lugng cua bgt ba mia sau tien xir ly cd the dudng hda hieu qua bing cic chi phim enzym cellulase khic nhau.
Bang mdt loat khio sit so bd, da xac djnh dugc ti dich (ti le ran:ldng) thich hgp l i 1:10, dam bao khi xii ly nguyen Heu ba mia phai dugc "ngap" trong chat Idng d miic tdi thiiu.
Cac nghien ciiu thu nhgn xenlulozo tir gd va nguyen lieu phi gd khiccho thiy, vol phuang di?n la mgt dung mdi hiru co, d nhirng dilu ki?n nhat djnh (nhiet do, thdi gian thich hgp) axit axetic cd kha ning hda tan lignin. Tuy nhien khi nang "trich ly"
lignin tir nguyen li?u thyc vat ciia axh axetic rit han che, phu thugc vio kich thude nguyen lieu, dilu kien xii ly. Vdi vai trd la tic nhan tach logi lignin, khi ning tham gia vao phan iing vdi lignin ciia axlt axetic cung khdng cao, ke ca khi sii dyng cac dang nguyen lieu de tach logi lignin, nhu miin vyn gd hay cay than thao. Mac dii co che hda hgc ciia qua trinh tach loai lignin bing axlt axetic cdn chua dugc nghien ciiu siu, nhung vin de su dyng cic axit vd co mgnh (H2SO4, HCl, HNO3) lam chit frg niu, dl tang cudng kha nang tich logi lignin ciia axit axetic, da chimg td hieu qui rd ret [4,13]. Ben canh dd, cho dii dudi tac dung ciia axit axetic va cic chit trg niu, lignin cd the bi phan hiiy, tgothanh cac hgp chit thip phan tli, nhung kha nang hda tan cua chiing trong dung djch axlt axetic rit han chi, vi vay de cd the tach chiing ra khdi xo sgi, cin phai cd cdng doan frn'ch ly kilm. Do dd qua trinh thu nhan xenlulozo tir ba mia, cung nhu cic dgng nguyen lieu khac, cin phai tien hinh hai cdng dogn: niu bing dung djch
TCHH, T. 53(1), 2015
axit axetic va frich ly kiem. Cac nghien cihi dugc cdng bd chua de cap nhieu den khia cgnh niy.
Trong sd ba loai axit vd co neu fren, axit nifric, dugc sir dyng trong phuong phip xac dinh ham lugng xenlulozo cua nguyen li?u thyc vit, la axit de dang tac dyng vdi lignin, tgo thanh cac hgp chit ciia nito (cic oxit) ddc hai, miic sii dyng cao va bi tieu hao trong qua trinh xu ly, vi vgy frong nghien ciiu niy khdng sii dyng.
Ket qua nau thir nghiemba mia vdi axit axetic bd sung axit sunfiirie va axit clohydric cho thiy, hieu qua ciia axit clohydric cao hon nhieu, so vdi axit sunfuric, vi vay da chgn axit clohydric lam chat trg nau cho cac nghien ciiu tiep theo.
3.1. Anh hudng cua muc sur dung hoa chat De xacdinh dugc miic sir dyng hda chat nau thich hgp, da tien hanh cac me niu vdi ti djch 1:10, d nhiet dg sdl ciia dung dich (khoing 115 °C), vdi thdi gian xii: ly keo dai 2 gid. Ti le CH3C00H:HC1 dugc dieu chinh d cac miic, tir 99:1; 98:2; 96:4 va 90:10 (% theo the tich). Bgt sau niu dugc tich thu hdi axit, nia nhieu lan rdi trich ly kiem bang dung dich NaOH 1% (pH = 12), vdi ndng do bgt 10%
trong 30 phut, sau dd nia va trung hda kiem, vat nude vi say khd de xac dinh hieu suit bdt vi phin tich ham lugng xenlulozo frong bdt.
Kit qua thu dugc (hinh 1) cho thiy, hieu qua phan dogn ba mia cua axit axetic khi sii dung dgc lap rit thap. Sy phin huy nguyen lieu dien ra khdng nhieu lam. O dieu kien nay, hieu suat bdt thu dugc sau nau tuong ddl cao (82,82 % so vdi nguyen lieu ban diu), sau xii ly bi mia gin nhu vin gift nguyen hinh dgng ban diu, chua phin sgi. Trong khi do, d cimg mdt dieu kien xir ly, sir dung ket hgp axit axetic vdi axit clohydric, da tang cudng kha ning phin huy cua nguyen lieu, bgt sau niu bj phan sgi manh, dich nau thu dugc cd miu den sim.
Quan sit su thay ddi ciia hieu suit bdt va hieu suit xenlulozo so vdi nguyen lieu ban dau (hinh 1) cd the thay, su phan hiiy ba mia dien ra nhanh trong khoang ndng dd HCl 0,5-1,0 % (theo thi tich), sau dd giam dan. Song song dd, hieu suit xenlulozo so vol nguyen lieu khd glim nh?, tii miic khoing 43 % xudng khoang 41 %. Dieu dd cho thay, frong dilu kien xii ly nay, cic axit cd inh hudng den thiiy phin xenlulozo va hemixenluloza. Trong trudng hgp khdng cd axit clohydric, thiiy phan xenlulozo diln ra khdng dang ke (chi khoang 0,5 % xenlulozo bi phan hiiy), cdn khi cd mgt HCl, mirc phin hiiy xenlulozo cd thi dat tir 1,9 % (vdi ndng do HCl 1,0 %) tdi gin 6,5 % (khi ndng dg HCl 5,0 %) so vdi khdi Iugng trong ba mia ban dau.
Nhd thuc day tich logi lignin cua HCl, mi cd thi
Thu nhdn xenluloza tir ba mia cho...
thu dugc bdt sau niu cd ham lugng xenlulozo cao hon nhilu, so vdi frudng hgp khdng sii dyng HCl d cimg nhi?t dg va thdi gian niu. Khi khdng bd sung HCl, ta thu dugc bdt hieu suit fren 80 %, vdi ham lugng xenlulozo chi dgt khoang 52 %. D I thu dugc bdt cd ham lugng xenlulozo khoing 80 %, cin bo sung HCl vdi nong dQ khoang 0,5 % trong dich nau, friong img ti le CHjCOOHrHCl la 99,5 %:0,5 % theo thi tich. Qua kit qua thu dugc cd the thiy, ndng do HCl thich hgp nhit la khoing 0,5-1 % (theo thi tich) hay ti le CH3C00H:HC1 thich hgp nhit la khoang (99-99,5) %:(1:0,5) %.
Hita lu^gHCl irong dicb nau (%Thea ThJticb)
Hinh I: Anh hudng cua mirc sir dyng HCl tdi hieu suit va tinh chat ciia bdt xenlulozo (1-Hieu suit bgt sau xii ly; 2-Hi?u suit xenluloza;
3-H^m lugmg xenluloza trong b^rt) 3.2. Anh huong ciia thdi gian xu ly
Khitien hanh xir ly ba mfa vdi dung dich axit axetic cd ham lugng HCl li 1 %, d cic miic thdi gian xii ly tir 30 phiit din 3 gid, da xie dinh dugc, trong khoang 1 gid niu, nguyen lieu bj phan hiiy manh, ket qui la hieu suit bgt giam tuong doi, keo theo hi?u suit xenlulozo so vdi nguyen lieu cung glim, cdn ham lugng xenlulozo frong bdt thi ting din (hinh 2).
De thu dugc bgt cd him lugng xenlulozo dgt tren 80%, chi cin thai gian niu khoang tren 30 phut, cdn thai gian niu keo dai qui 2 gid se khdng hi?u qua, do xenluloza se bi phan hiiy mgnh. Vi vgy, dl thu dugc bdt cd ham lugng xenlulozo 80-85 %, thdi gian niu thich hgp frong khoang 0,5-1,5 gid, tiiy theo yeu ciu vl chit lugng bgt.
3.3. Anh huong ciia nhiet do xu* Iy
Kit qua thu dugc (hinh 3) tir mgt loat me niu vdi ti 1? axit va thdi gian nau nhu nhau, d cac chi dp
TCHH, T. 53(1), 2015
nhi?t khac nhau cho thay, tuong quan giiJa hieu suit hot, ham lugng xenlulozo trong bdt v i hi?u suit xenlulozo so vdi nguyen lieu, cd the md ta bang phuong trinh ham bic 3. Nhin chung, d nhiet do dudi 100 °C, sy phin hiiy xenluloza l i khdng dang kl. D I thu dugc bgt cd ham lugng xenluloza khoang 80-85 %, nhiet dg niu thich hgp la 80-90 °C.
Thcnp>a
Hinh 2: Anh hudng cua thdi gian niu tdi hieu suit va tinh chit cua bdt xenlulozo (1-Hi?u suit bpt sau xii ly, 2-Hieu suat xenlulozo;
3-Him lugng xenluloza trong b§t)
i i
Le Quang Dien va cdng su niul:10; ti le CH3C00H:HC1 (% thi tich): 99:1;
nhiet do niu: 80-90 "C v i thdi gian niu: 60-120 phiit. Bdt xenlulozo thu dugc cd hieu suit 51-53 % so vdi nguyen lieu, vdi ham lugng xenlulozo trong bdt dgt 80-85 %.
Xac dinh hieu suat ciia bgt sau cdng doan 1 va sau trich ly kiem (cdng dogn 2) ddi vdi mdt vai me nau cho thay, hieu suat bgt sau cdng dogn 1 dat khoang 56-58 %, cdn sau trich ly kiem trung binh 51-53 % so vdi khdi lugng ba mia ban dau. Nhu vgy cd the thiy, trong cdng doan trich ly kiem cd khoang vii phan tram cac thanh phan cua bgt, chii yeu la lignin bj hda tan, nhd dd tinh chat cua bgt da dugc cii thien ding ke. Da xac djnh dugc, d cdng doan I cd khoang 73-74 % tdng lugng lignin ban dau cd trong ba mfa bj hda vao dung djch axlt, tiic lugng lignin cdn lgi trong bgt sau cdng doan xOr ly bang axit vio khoing 26-27 %. Trong cdng dogn trich ly kiem, lugng lignin dugc hda tan tuong ung la 17-19
%, lugng lignin cdn lgi trong bdt chiem 8-9 % tdng lugng cd trong ba mfa ban diu.
3.4. Toi uu hoa cong doan trich ly kiem DB hda tan khoang 1/5 lugng lignin cd trong ba mia ban dau, tieu hao NaOH cho cdng doan 2 tuong duong gin 5 % so vdi nguyen lieu. Vdi myc tieu giam tieu hao hda chat, nhiet do va thdi gian cdng doan trich ly kiem, da tien hanh nau me ldn bdt frong thiet bj niu dung tich 5 lit, theo che dg cdng ngh? thfch hgp, thu dugc ket qua tren bang 1.
Bdng 1: Ket qui niu bgt trong thiet bj pilot
Hinh 3: Anh hudng ciia nhl?t dg nau tdi hieu suit va tinh chit cua bgt xenlulozo {I-Hieu suit bOt sau xii 1^; 2-Hi?u suit xenluloza;
3-H^ lupng xenlulozo trong bpt) Tir cac kit qui thu dugc cd thi thay, vdi che do cdng ngh? trich ly kilm sau niu cd dinh (xir ly bang dung djch NaOH 1 % cd pH =^ 12 vdi ndng do bdt 10
%, d nhiet dg sdl trong 30 phiit),che dg cdng nghe niu thich hgp (cdng doan 1) nhu sau: ti dich
TT 1 2 3 4 5
Thong so Khoi lirgng nguyen lieu (g) CH3C00H(Iit) HCl (ml) Hieu suat bgt (%) Dich den thu dugc (lit)
Me nau 1 107,2 4,95
50 56,8
4,1 2 85,0 3,90 40 55,5
3,2 Bdt thu dugc cd ham lugng lignin 12,3 %, ham lugng xenlulozo 71,4 %, dadugc sii dung cho nghien ciiu frich iy kiem: tiln hanh xii ly bdt (moi lin vdi khoang 5 g bdt khd gid) d nhiet do sdi, frong 30 phut, vdi ndng do bgt 10 %. Ndng dg dung djch NaOH dugc thay ddi d cac miic 0,25 %: 0,5 %; 0,75 % va 1,0 %. Ket qua (bang 2) cho thay, de thu dugc bdt cd ham lugng xenluloza dat > 80 % nhu muc tleu, cd thi sii dyng dung dich NaOH ndng do 0,75-1,0 %, tuang duong miic sir dyng 6,7-9,0 % NaOH so vdi bgt hay khoing 3,8-5,0 % so vdi ba mia chua xir ly.
TCHH, T. 53(1), 2015
Bdng 2: Kit qua frich Iy kilm bdt sau niu bang dung dich NaOH ndng do khac nhau 1
2 3 4
N6ngdgNaOH(%) Hieu suit bpt (%) Ham lugng xenlulozg trong bgt (%) Hieu suat xenlulozo
(%)
0,25 57,16 72,11 41,21
0,5 54,91 76,76 42,14
0,75 53,02 80,06 42,45
1,0 52,10 82,44 42,95
Cac mau bdt ba mfa da qua tien xir ly dugc thu nghiem dudng hda bang enzym thuong phim Cellic®CTec2, cho hieu suit dudng khu tuong img lin lugt la 20,6; 27,2; 36,2 va 38,0 % so vdi ba mia.
d che dg trich ly kiem thich hgp, hieu suit dudng khli dgt 82-86 % so vdi xenluloza. Phan tich ba edn lai cho thay, gin 100 % xenlulozo da bj dudng hda.
Tuong tu, khio sit qua frinh frich ly kiem d nhi?t dg trong khoang 60-100 °C cung cho thiy nhiet do thich hgp cd the duy tri frong khoang 80-90 °C.
Nhu viy, chi dg cdng nghe trich ly kiem thich hgp nhu sau: nong dg NaOH: 0,75-1,0 %; ndng do bpt: -10 %; nhiet dg xii ly: 80-90 "C; thdi gian xii Iy: 30 phut.
Dang chii y la dung djch NaOH sau trieh ly vin cd pH xip xi 10, vi vgy cd kha nang til sii dyng dl giam tieu hao hda chit.
Ve khi nang thu hdi va tai sir dung axit, da nghien ciiu thu hdi axit bang phuang phap chung cit ehan khdng, so bd d dilu kien phdng thi nghiem ed thi thu hdi dugc > 76,5 % axit da tieu hao, cd thi tai su dung va cho hi?u qua qua frinh dat tuong duong.
Ben cgnh dd, sau khi chung bdc thu hdi axlt, cdn thu dugc mdt lugng lignin tuong ddi, cd thi tin dyng.
4. KET LUAN
1. Xii ly ba mia bang axit axetic cd bd sung axlt clohydric d nhi?t dg thip, Ii phucmg phap phii hgp de thu nhgn xenlulozo, cho sin xuit etanol sinh hgc.
2. Chi dg cdng ngh? thich hgp dl thu bdt xa sgi hi?u suit 51-53 %, cd ham lugng xenlulozo 80-85 % bao gdm hai cdng dogn xir ly:
- Cdng doan I: bang axit axetic cd bd sung axit clohydric vdi ti le axlt tuong irng 99:1 theo thi tich;
ti djch 1:10; d nhiet dg 80-90 "C, frong 60-90 phiit;
- Cdng dogn 2: bing dung dich NaOH ndng dd 0,75-1,0 %, d nhi?t do 80-90 °C, frong 30 phiit.
3. Ngoai xenlulozo ra, cdn cd the thu hdi va tan dyng lignin.
Ldi cam on. Nghien cuu dirge thuc hiin bdng hd trg hnh phi tir Be tdi KHCN 05.HB-DT.05.13/NLSH
"Nghiin ctiu cdng nghe tiin xir ly ba mia thdn thiin
Thu nhdn xenluloza tirbd mia cho...
mdi truang img difng trong sdn xudt etanol", thifc hien ndm 2013-2014.
T A I LIEU THAM K H A O
1. LS Mai Oanh, DoSn Thii Hoa. Nghien ciiu dnh hudng cua xir If sa bg phe lieu go bgch dan urd trong moi trudng kiem tdi thuy phdn bdng enzym cho sdn xudt etanol nhien lieu, Tap chf KH& CN Cac Trucmg Dgi hpc Ky thuat, S6 80, y. 89-92 (2010).
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\iT!^\Mmospheric acetic acid pulping of rice straw Lien hi: Le Quang Dien
Trudng Dai hgc Bich khoa Ha Ndi S6 I, Dal Cd Viet, Hai Ba Trung, Ha Ngi E-mail: [email protected] Dien thoai: 0438684955, 0914300930.
