Vietnam Journal of Physiology 17(1), 4/2013 ISSN: 18S9 - 2376
Establish the animal models ef ketamine (dose 0.3 mg/kg/day, intramuscular) schizophrenia by using drugs effecting on in monkeys increased locomotion alter 10 glutamatergic systems such BS phencycildine, minute injection. But » caused increased ketamine MKBOI were considered by many locomotion and reduced social interaction with msean>h'ers Our study te assess tha others alter 14 days consecutively. Present subchronic effects of ketamine on social model investigated social interaction on behavkirs of monkeys as a basis for an animal monkey by using subchronic ketamine showetl model of schizophrenia in monkey 8 monkeys symptoms similar to the negative symptoms in (macaca mulusta, 4 males end 4 females, schizophrenia patients,
weighed 4 te 6 kg) were divided randomly into Key words: ketamine. monkeys, social 4 pairs, ii/lonkeys were injected with ketamine interaction, locomotion, schizophrenia, (dose 0.3 mg/kg/day, intramuscular) for 14 This work was supported by Ihe Asian consecutive days. Locomotion and social Core Program, JSPS, Japan and the Vietnam behaviors of monkeys were analyzed before Ministry of Science and Technokigy and alter Injection. Results showed that (2643/QB-BKHCN)
OAP LKNG K H 6 N G GIAN C O A HACH HANH NHAN:
VAI T R 6 LU'U T R O TRI NH6I DAI HAN (3 CAP DO NEURON
T r i n Hai A n h ' , N g u y i n L6 C h l S n " , NguySn Duy B j c ' , Hisao N I s h l j o "
^Hpc vi^n Quin y 'B9I hgc Toyama
^Chuvmg trinh Asian Core, JSPS, Nh^t Bin Nhan bSi 11/3/2013; siia bSi 25/3/2013; chSp nhSn dang 15/4/2013
/Wuc tidu cua nghiin cdu niy li dinh gii vai trd cda h^ch hanh nhin trong tri nhd kiidng gian thdng qua cic bii tip vi inh hudng cue cic vit chuin trong mdi truing. Ddi tuqng nghiin cdv li chudt nhit tring duoc dm cic diin cuv vio bd gida nio trudc di kich thich vi cic diin cue vio vdng hanh nhin di ghi dip dng cda neuron. Khi nghiin cuu. chudt du^ nhdt trong mdt hdp hinh tru cd gin camera di ghi lai vin ddng cua chudt. Dip Ong cua cic ti bio dinh vi, heat tinh diin vi hoat ddng v$n ddng cda chudt duoc s6 hda vi tnjyin vi miy tinh tmng tim.
Nghiin cuv cho thay chf cd mdt tf li nhd neuron trong hach hanh nhin li neuron dinh vj; cic ti bio niy chju inh hudng manh cue cic v$t chuin gin vi dip dng chi rd rdt vj tri trong mdi trudng quen thudc. TU kit qui trin gt?! y ring hach hanh nhin tham gla vio luu gia tri nhd dii han vi khdng gian, vi trong mdi quan hi vdi hdi hii mS, h^ch hanh nhin ddng vai trd nhu mdt hi tham chiiu gidp cho hdi hii mi cd thi se sinh vi flip nhin ducrc cic thdng tin mdi tmng mdi tru&ng.
TO' khda: Hach hanh nhin, hdi hii mi, neuron djnh vj
1. DAT VAN Ofe d$t quy, c h i n thu'ang nao, Parl<inson, stress, Ke tCr nam 1953 vd\ bSo cdo ban d4u vh hOi chiJng Wernid^e-KorsakofTs ...iu6n \d chO trufrng h(?p b^nh ly cua b$nh nhSn HM [9], tri ah nghi6n cilu hdng d i u oua t h j n ldnh hi)0 n h * cung vSn d4 ISO hda hay c i c tlnh trsng cOng nhu' cda nhi4u ngSnh l(hoa hpc khic b$nh l:^ gSy r6i logn tri nh6 nhif Alzheimer's, nhu- b$nh ly hpc, duuc hpc, v.v. Thu$t ngQ" t r i
nhd'" thud'ng dk c | p td-i khS nSng nhd lai mpt cdch c6 y thiJc vfe nhO'ng thdng tin, s y ki$n d5 du'p'c trai nghidm ho$c duvc hpc trong qud khi>. Loai tri nhd ndy du'p'c cho Id phy thuOc ch$t chg vdo hdi hai mS (hippocampal fomiation) vd cde vOng nSo lifin quan [5], [6], [81. Y vdn cOng dd kh^ng djnh h i i hgnh nhdn (Amygdala) Id tiidnh phSn ndng e6t tham gla vdo cdc chLFC ndng c3m xue cua nSo bO [4], {8], vd d i k hda hoat dOng cua h6i hai md [2].
N h i k nghlfin ciJu dS khao sdt m6i quan h§
giO'a hgeh hgnh nhdn vd h i i hai md trong qud trinh hpc tdp vd tri nhd- [2] nhung cdn it nghifen cO-u vk tri nhd' khfing gian md h6l hai md e6 vai trd quy4t djnh trong tri nhd v^
khflng gian. VI vdy, nghiSn ei^u ndy du'p'c ti4n hdnh vdi myc dich ddnh gid vai trd cua hgeh hanh nhdn trong tri nhd khdng gian thfing qua cdc bdi tdp v^ 3nh hu'o'ng cua cac vdt ehuin trong mfil tni'd'ng.
2. D 6 | TU'ONG VA PHlfONG PHAP NGHIEN CO'U
2.1. Q6\ tu*p>ng nghlSn cij>u
Nghi6n ci>u dut^c ti^n hdnh trfen hai loai chufit nhit C57BL6 10-12 tu^n tu6l, thfe trpng 23-26 gr. Ofing vdt do Trung tdm ddng vgt, tafdng dai hgc Toyama cung c^p vd du'p'c dn u6ng day du vd nufil trong di^u ki#n phdng thi nghifim.
2.2. Phdu t h u i t cdm di^n cu-c
Bfing vdt du'p'c gdy mfi bdng pentobarbital, li4u 40 mg/kg t h i trpng vd Surge ddt edc di$n eye kieh thleh vd dl$n cu'c ghl vdo ndo. BlSn cu-c kich thleh nh^m cung e l p cdc phin thud-ng t y kich thich cho d0ng vdt thyc hien bdi tdp, c^u tao gdm hai dl^n eye den bdng fh6p khdng rl, dud'ng kinh 100 pm, du'gc c l y vdo bd giQ'a ndo tru'd'c tgi sau b6n cua vCing dudi doi thi ca 2 b6n bdn c l u (tpa dfi trydc- sau: -2,3 mm; trong-ngodi; ±0,7+0,75 mm va xu6ng dudi 5,3+5,4 mm so vdi d i l m Bregma) [1]. Trong qud trinh thi nghifim, di^n eye ndo tgo duvc ddp ii'ng t y kich thleh tot hon se du'OC SLF dyng. Ddng di#n kich thich dudi dgng xung vudng cd eudng dO tu' 80-90 pA,
t i n s6 80 Hz vd thdi gian kieh thleh k6o ddi 0,5 gidy.
BO di§n eye ghi bao g6m 8 di$n eye dan (017 pm, Califomia Fine Wire Company, M?) duye thilt k l d l cd t h i di chuyin cOng dup'c cdy vdo vung hanh nhdn [1], (tpa dfl. trudc- sau: -1,34 mm; trong-ngodi: ±2,8 mm; xuing dudi: 4.2 mm). Cdc di$n eye ndy trudc dd dd dup'c ma vang d l flam bao dl0n trd dl$n eye giam xuing cdn 100-300 k f i tgi t i n s6 1 kHz vd duyc ddt trong mflt I n g th6p khfing rl cd 26 gauge nhim bao v$ vd dam bao cdc di$n cue duyc ddt vdo dung viing c i n ghi.
2.3. Phuo'ng ti#n nghien ciru vd bai t|ip Nghien cCru duyc t i l n hdnh vdi cde dyng cy g6m mflt hflp hlnh try md (dudng kinh 80 cm, thanh cao 25 cm) duyc d§t tr6n mflt khung nho cao 80 cm so vdi m|t sdn, duyc g i n bdnh xe d l ed t h i quay duoe trong cdc dieu ki^n thi nghifim (hlnh 1A). Mdt trong cua hfip try duyc so'n den vd duyc ddt giCra mot khung phu r6m kin (dudng kinh 180 cm, cao 200 em). Tr6n trin, sdt ngodi ria cua khung cd g i n 4 loa, cdch nhau 90° vd phdt dm don (vifhlte-nolse). Bfin canh cdc loa g i n 4 bfing ddn syl d i t 40W vd trung tdm eua khung duye g i n mflt camera de ghi Igl hogt dfing cua dflng vat trong hflp tru. Trong dilu kien thi nghiem binh thudng (baseline), d6n duye bdt d vl tri 9h vd loa phdt dm don d vi tri 3h vd chijng se dong vai trd Id edc vdt chuin xa (distal cue). Bfin cgnh dd, mflt dfin LED nho cQng duoc gdn tren d l u ddng vdt. Camera sS ghi igi cac di chuyin cua dflng vdt dya trfin vj tri cua dfin LED, so hda hinh anh thu duyc vd tnjyIn v l mdy tinh trung tdm. Mdy tlnh (Deil precision T3400) t i l p nhdn tpa dfl x, y ve vl tri cua dfing vdt vdi t i n s6 33 khung hinh/gidy.
Dflng vdt duye hudn luy$n thue hifin bdi tdp tlm k i l m phan thudng n g l u nhifin trong hfip tnj md (hinh IB). Vdi bdi tdp ndy, mdy tinh se djnh ra mflt vCing p h i n thudng vdi tam duyc xac djnh ngau nhifin vd nam trong mfit hlnh vufing ngogi t i l p vdi hfip try, dflng vdt se duyc nhdn mflt p h i n thudng khi nd di vdo
Vietnam Journal of Physiology 17(1), 4/2013 ISSN: 1859-2376
viing da dupc dinh s i n nSy, Sau khi kich m6i s6 dupc Ura chpn vi kich hoat sau thleh da dupc phat, mpt vOng phSn thudng khoSng nght 5 glSy.
A So' do ti& thong Ihi ngliifim
"ii"
B. Bai tap tim phan thudng nglu nhien
C. Lu-gc d6 qua Irinh thi nghiem Irong moi Iru-ong quen thupc Quay vgt cliiian xa Ouay vflt chu^n g ^
D. Luac d6 qua trinh thi nghifm trong moi trudng la Quay vgt chuSn Ka Quay vai chuan g^n
b 9<=
•Q 0'''0
Hinh 1. So do hfi thong thi nghifim (A), bdi tdp tim p h i n thudng n g l u nhifin (B) vd luo-c d l qua trinh thi nghifim trong mfli trudng quen thufic (C) vd mfii trudng la (D).
2.4. Phdn lap va ghi dien t h i neuron Bfi difin eye ghi se duyc t i l n sdu xuing mfil -20 [jm/ngdy. Trong khi dflng v$t thyc hj$n bdi tdp, vifie xde dinh phCre bfl sdng neuron djnh vj cQng nhu ghi lgi di0n t h i hogt dflng cua neuron duuc t i l n hdnh theo cdch thCrc dd duyc mifiu ta trong nhilu tdi II$u trudc ddy [7], [10] (hinh 2),
2.5. Cdc b u ^ thi nghiem trong mdi t r u i n g quen thuoc va moi t r u i n g m d i
Ddp U'ng eua neuron duo'c theo ddi vd ghi lgi trong suot qud trinh thi nghi§m d ca 2 mfii
trudng quen thuflc (dfing vdt duyc tilp xiic hdng ngdy) l l n trong mfii trudng mdi Ig (dflng vdt duoc a l p xuc l l n d l u tifin). T i n g e0ng c610 bdi tdp, m&i bdi t§p kdo ddi 10 phijt vd sgu mli bdi tdp, dfing vdt duye nght 5 philt trudc khi budc vdo bdi t§p tilp theo Trong m l i mfli trudng, anh hudng cua vi$e thay d l i cdc vdt chuin g i n vd xa l l n luyt duac kilm ehdng.
Trude tifin, trong mfli trudng quen thuflc, d bdi tdp co ban, hogt dflng cua neuron duyc ghi lai trong khi dflng vdt thyc hi$n vi§c tim kilm phin thudng trong mfii tardng vdi loa vd dfin bgt a vj
tri 9h vd 3h. Di§n t h i neuron duye ghi t i l p trang cdc bdi tdp quay vdt chuin xa vd g i n . Cf bai tdp quay v^l chuin xa, dfin vd loa duyc quay mflt gfic 180° trong khi hflp tn,i duye giO khflng d l i . Nguye Igi, trong bdi t$p quay vdt chuin g i n , hflp tn,i quay 180° cbn cde v$t chuin xa duye giO' c6 d|nh. GiOa cdc bdi t$p quay vdt chuin Id edc bdi tdp md tgi dd cde vdl chuin duyc quay trd v l nhu d bdi tdp ca bdn (bdi tdp cc b i n 2 vd bdi t i p CO bdn 3), (hlnh IC).
Trong mfli trudng mdi, thu t y cdc budc thi nghi$m eung tuang t y nhu trong mfli trudng quen thufic (hlnh ID) nhung hogt dflng cua neuron duye theo dfii khi dflng vdt duyc t i l p
XLie vdi hflp fry mdi. Hfip try ndy ed hinh vufing, kich thude 55x55 cm, cao 25 cm duye son den bfin trong vd dtJng thay t h i cho hflp try trfin dS mfi ta d trfin. D l dam bao hflp try vufing luOn dfing vai trd Id mfil trudng mdi. ed 2 hfip try vufing vdi thilt k l nhu nhau duac su dyng ludn phifin vd trudc m i l bdi tdp trong cd mfii trudng quen thuflc eOng nhu mfli trudng la, sdn cua cdc hflp try duyc lau bdng dung djch Habltan 0,5% (cua hdng Sumlmoto) d l giam thilu vai trfi ffinh hudng khfing gian cua khuu gide cd t h i Idm lu md vai trd cua cdc vdt chuin xa vd g i n duyc sir dung trong thi nghifim ndy.
Hinh 2. Difin t h i hogt dflng cua mfit neuron dinh vi thugc hgeh hanh nhdn ghi duac theo phuang phdp monotrode, (A) Hlnh dgng t i n g hyp cdc sdng cua mflt neuron duyc gfip lai dya vdo gid trj difin t h i cua moi i i n khu eye dat dien the ngudng nhit djnh. (B) Phue bfi xung (complex-spike) eua neuron mang dac tinh tuang t y phue bfi xung cua neuron ffinh vi vung hai md. (C) Sy tuang d i n g v l cdc ddc tinh cua sdng thong qua cde cluster cho thly cdc xung thu duyc cijng phdt ra tir mflt neuron.
2.6. D i ^ g hinh vCrng phdt xung Mdi tnj'dng d u y c chia thdnh cdc fi vufing c6 kieh thudc m l i canh 2,5 cm [10]. Ban do phdt xung eua neuron djnh vj d u y c Idp b i n g cdch chia t i n g s l l l n phdt xung cua neuron dd tgi mflt fi vufing n h i t dinh cho t i n g thdi gian dfing vdt di qua 6 dfi vd duyc b i l u d i l n theo thang mdu. Cde fi md dfing vdt khfing di tdi d u y c b i l u d i l n bdng mdu xanh, cdn edc 6 dfing vdt dd di qua nhung neuron
khfing phdt xung dwgc b i l u d i l n bdng mdu trdng Cdc ty s6 phdt xung Idn han duyc b i l u d i l n bdng thang mdu tdng d i n theo thd t u mdu xdm. xanh lam, xanh la, vdng vd dd Trong dfi, cdc fi c6 ty s l phdt xung cao gap t l i t h i l u 2 l l n t i n s l phdt xung trung binh duye b i l u thj bdng mdu dd. VOng phdt xung duyc tgo nfin bdi (1) cdc fi c6 ty so phdt xung cao han ft n h i t 2 l l n tan sfl phdt xung trung binh trong mfii mflt bdi tdp; vd
Vietnam Journal of Physiology 17(1), 4/2013
ISSN: 1859-2376
(2) cdc fi ndy phdi n i m t i l n k l 2 cgnh cua nhau (khfing tlnh cdc fl t i l p gidp vdi nhau d cde gde). D i n g thdi (3) m^t vCing phdt xung chl duyc dua vdo tlnh todn d cde budc t i l p theo n l u kieh thude eua nd dgt t u 9 0 trd Ifin; vd (4) n l u cd thfim cdc 0 g i n dfi thda mdn cdc tifiu chl trfin cCia vung ddp dng thi nd se d u y c col Id mfit phSn vOng phdt xung.
Ddp dng cua neuron d l i vdi cde d i l u ki$n thay d l l cCia mfii trudng se d u y c so sdnh thfing qua s y thay d l i v l vj tri cua cdc vung phdt xung (hinh 3) Mflt neuron d u y c xdc djnh thay d l i theo y l u t l cua mfii trudng khi vd chi khi vung phdt xung cua nd quay tuang dng vdi gde quay cua cdc vdt ehuin trong mfii trudng (it n h i t Idn han 90° khi vdt chuin quay 180°) hode vCing phdt xung b i l n m i t khi vdt c h u i n thay d l i hay khi dflng vdt duyc t i l p xue vdi mfli trudng mdi.
Cdc s6 Iifiu d u y c so sdnh, t h i n g ke bdng p h i n m i m Microsoft Office Excel 2007.
3. K^T QUA NGHIEN CU'U 3.1. T^ 1^ neuron dinh vj
Bang 1. Ddp dng cua neuron djnh vj hgeh
Trong t i n g s l 129 neuron ghi duyc d hgeh hgnh nhdn, cd 11 (8,5%) neuron djnh vj, 1 (0.78%) neuron ddp Crng lifin quan tdi hudng d l u cua dflng v$t, cdn Igi Id cdc ddp ung khde (lifin qugn tdi p h i n thudng, dnh sdng, dm thanh) vd ddp i>ng khfing dde hi§u.
Trong nghifin ci>u ndy, chdng tfli cht di sdu phdn tich ddp t>ng cua cde neuron djnh vj.
3.2. 0$c tfnh neuron djnh vj c t o hgeh hanh nhdn
Cde neuron d u y c xdc djnh thuflc loai t l bdo djnh vj (xult hi$n ede phd'c bfl xung duyc mfl td d trfin, hlnh 28) sfi l l n luot duyc theo dfii trong edc d i l u klfin khdc nhau v l vdt chuin trong mfli trudng g i m quay vdt chuin xa vd vdt chuin g i n trong mfli trudng quen thufic vd trong mfli trufi'ng mdi md dfing vdt duyc t i l p xue l l n ddu tifin. Bdp tjng ciia neuron d l i vdi mflt dieu k l | n n h i t dinh bao g i m vung ddp ung se quay tuang ung theo vi$c quay vdt chuin hode nfi se ngirng phdt xung (mdt vung ddp Crng). Ddp dng aJa U neuron duye t i n g hyp trong bang 1.
hgnh nhdn dudt cdc tdc dflng cua mfli tnrdng MOI trif&ng
(n, %) Quenttlu§c(11, 100) M i l l s (2, 100)""
Khong d^p LPng n {%) 1 (9,1)
0(0)
Vat chudn xa n(%) 0(0) 0(0)
Vat chudn gSn n(%) 9(81.8) 0(0)
Cahai n(%) 1 (9,1) 2 (100) , p < 0,001. / test gi&a ddp ung cua neuron vdi v$t chudn gin vd vdi cdc lo^i ddp &ng khdc trong mdi trucrng quen thudc *"*, p < 0,001, / test gi&a sd neuron ddp &ng tmng mdi tmimg mdi vd sd td bdo ddp ung trong mdi truing quen thudc.
K i t qua t u bang 1 cho thly cdc neuron i^nh v| deu cfi vCing phdt xung trong mfit tnj'dng quen thuflc (11, 100%); tuy nhifin trong mfli trudng mdi Ig, chi cfi 2 neuron x u l t hifin vung phdt xung (d mfil trudng quen thuflc todn bfi 11 neuron ddp dng so vdi d mfli trudng mdi chi cfi 2 neuron ddp dng. p <
0,001). Chi t i l t han, trong mfli trudng quen thuflc, viee quay vdt ehuin g i n tgo ra ddp dng d da s6 cde neuron {9 neuron ddp ung vdi vdt ehuin g i n (hinh 3, A4); 2 neuron cd ddp dng khde, p < 0,001). Cfin Irong mfli trudng mdi Ig, khfing nhOng s6 luyng neuron
xult hi$n vung ddp dng chilm tJ Ifi thlp (2 neuron) ma ca 2 neuron ndy d l u d l ddng bi tde dflng bdi cdc thay d l i trong mfli trudng.
Thyc t l qud trinh thi nghifim cho thly. trong mfii trudng mdi, da phan cdc neuron i^nh vi d l u khflng cd vung ddp ung (hinh 3, B6) hodc n l u cd thi cOng chl cfi vung ddp ung vdi kich thudc nhd vd khfing I n djnh, d l ddng bj thay d l l hode b i l n m i t trong edc bude thi nghl§m.
Tuy nhifin, do s l luyng neuron i^nh vj thu d u y c cdn hgn c h l nfin trong khufin k h i bdo cdo ndy, chijng tfii chua dua cdc s6 iieu Ihu d u y c vdo so sdnh, t h i n g kfi sdu han.
Bai lip CW b,in ? Qjay vai chuan gl
1 •'^
Hinh 3. Ddp ung cua mflt neuron dinh vj hgeh hgnh nhdn vdi cdc d i l u ki$n trong mfil trudng.
Mfii trudng duye chia thdnh cdc fi vd t i n s6 phdt xung trung binh cua neuron ung vdl mfii fi duye bilu d l l n theo thang mdu d bfin phdi cua hlnh; vCing ddp dng gom tdp hyp It nhit 9 dilm anh mdu dd lifin t i l p cfi t i n s6 phdt xung dgt t i i thllu 2 lln t i n s l trung binh. (A) Ddp dng trong mfli trudng quen thuflc, vific quay vdt chuin gdy anh hudng tdi dap dng ciia neuron thfing qua thay d l i vj tri cua vung ddp ung duyc ehCi y bdng khung vufing. (B) Ddp ung trong mfii trudng mdi Ig, trong trudng hyp khflng cfi vCing ddp ung trong bdi tdp ca ban 1, thi nghifim se ngdng lai md khfing tifin hdnh edc budc t i l p theo nhu trong mfli trudng quen thuflc.
nay, Mdt khdc, mfit chdc ndng n i l bdt cua hli 4. B A N L U A N
Ty 1$ neuron dinh vj
Cdc neuron dinh v| tgi hach hgnh nhdn chilm ty 1§ t h l p (8,5%) vd k i t qua ndy tuang ding vdl bdo cdo trude ddy [7] Tuy nhifin, s6 luyng edc nghifin cdu di sdu vdo ddnh gtd ddc tlnh khflng gian cua hgeh hgnh nhdn cdn rlt han chfi, cfi the bdi s l luang neuron djnh Vl khfing cao, viec thilt ke bdi tap khflng thdt die hi#u Ddu vay. tCr k i t qua cua nghten cdu ndy vd bdo cdo trudc day cua Pratt vd es. [7]
khdng dinh hach hgnh nhdn cd vai trfi trong tri nhd khflng gian
Bgc Unh khong gian cua hach hanh nhdn Ddp ung cua neuron hach hgnh nhan duac chia tiianh cdc nhom: khflng ddp dng vdi vific quay vat chuin; ddp dng vdt quay vdt ehuin xa (dfin vd loa); ddp ung vdi quay vdt chuin g i n (quay hflp try) vd dap dng vdi ca hai logi v§t chuin. Trong mfii trudng quen thufic, todn bfl cdc neuron dinh vj deu cd vung ddp dng rfi rang, tuang d l i 6n dinh trong suit qud trinh thi nghifim vd da s l cdc t l bdo ndy chju anh hudng bdi vdt chuin g i n han Id vgt chuin xa. Hach hanh nhdn tham gia mdt thilt vdo qud trinh cung c l tri nhd, luu gid tri nhd ddi han [8] vd cd nhilu dudng d i n truyin hudng tdm vd ly tdm giO'a h l i hai md vd hgeh hgnh nhdn [2] khing djnh moi lifin quan mgt thilt giQ'a glai p h l u vd chdc ndng cOa hai vung
hat md id phdt hlfin nhung thfing tin mdi trong mfli trudng [3] nfin t u k i t qua eua thi nghifim ndy cho thly hgeh hanh nhdn dufing nhu dfing vai trfi Id mfit hfi tham chilu, luu giu hinh anh quen thufic trong khfing gian cfin hoi hdi md se so sdnh cde thflng tin thu thdp duyc d l tu dd tim ra cdc thfing tin mdi trong mfii trudng. Mfit khi xult hlfin thong tin mdi, qud trinh luu gid thong t n se duyc kich hoat vd thfing tin mdi se ddn duae eung e6, luu trtr tgi nhifiu vCing nao khdc nhau bao gom hgeh hgnh nhdn Nhdn djnh nay duyc cung c l chle ehln han khi cho dong vdt tilp xuc vdi mfii trudng mdi. Do thong tin thu nhan duyc chua duyc luu tru trong hgeh hanh nhan nfin h l u nhu todn bfi cdc neuron dinh v[ d l u khflng phdt xung trong mfli trudng mdi hodc nlu ed thi vung phdt xung cQng nho vd khflng I n dinh, d l bi thay d l i bdi sy thay d l i k l tilp cOa mfii trudng nhu quay vdt chuin gan vd/hodc vdt chuin xa Do ty ifi neuron dinh vj hgeh hanh nhdn chilm ty Ifi khflng cao nfin s l luang t l bdo duac dua vdo phdn tich trong bdo cdo ndy cdn hgn chl. Tuy nhifin, vdl nhung k i t qua trfin se la mflt hudng mdi nhilu triln vpng d l tilp tyc nghlfin cuu hilu sdu han v l vai trfi luu tru thfing tin cua hach hgnh nhdn trong m l i tuang quan vdi h l i hai md nfii nfing cQng nhu nhieu vCing ndo khdc noi chung.
Vietnam Journal of Physiology 17(1). 4/2013 ISSN: 1 6 5 9 - 2 3 7 6
5. K £ T LUAN
Hgeh hgnh nhdn tham gia vdo qud trinh luu giO' tri nhd ddi hgn v l khflng gian, ddng vat trfi nhu mflt h$ tham chilu giup cho h i i hai md cd t h i so sdnh vd t i l p nh$n ede thflng tin mdi trong mfii trudng s6ng.
TAI LI$U THAM KHAO
1. Franklin K B J , Paxinos G (1997) The mouse brain in steriotaxic coordinates.
Sandiego: Academic.
^. Klm EJ, Kim ES, Park M, et al. (2012) Amygdala stimulation produces alterations on firing properties of hippocampal place cells. J Neurosel. 32(33)11424-11434.
3. Knight RT (1996) Contribution of human hippocampal region to novelty detection.
Nature 389.256-259.
-•. LeDoux JE (1993) Emotional memory systems in the brain. Behav Brain Res.
58:69-79
5. McEwen B, Sapolsky RM (1995) Stress and cognitive function. Curr Opi Neurobiol.
5:205-216.
6. O'Keefe J , Nadel L (1978) The hippocampus as a cognitive map Oxford University Press,
, . Pratt WE, Mizumori SJY (1998) Characteristics of basolateral amygdale neuron firing on spatial memory task involving differential rew/ard. Behav Neurosel. 112(3),554-570.
8. Riehter-Levin G, Akirav I (2000) Amygdala-Hippocampus dynamic mteractioin In relation to memory Molecul.
Neurobiol, 22:11-20
^. Scoville WB (1953) The limbic lobe in man, J. Neurosurg. 11(1)-64-66 lO.Tran AH, Uwano T, Kimura T, et al.
(2008) Dopamine D l receptor modulates hippocampal representation plasticity to
spatial novelty. J Neurosci. 28(59)13390- 13400.
SUMMARY Amygdala responses t o spatial manipulation: role in long-term memory at
neuronal stage
Tran Hai A n h \ Nguyen Le Chien^, Nguyen Duy Bac', Hisao Nishijo^'
^Vietnam Military Medical Unh/ersity
^Toyama University, Japan
^Asian Core Programe, JSPS, Japan Received Mar. 11, 2013; revised Mar. 25, 2013; accepted Apr. 15, 2013 To study the role of amygdala in spatial memory through tasks of environmental cue manipulations, this study had been conducted on mice with recording electrodes at amygdala to monitor neuronal action potentials there and stimulating electrodes at middle forebraln bundle serving for electric stimulation rewards In experiments, a CCD camera monitored and recorded mouse locomotions within open-helds and gathered neuronal data were digitalized and synchronized in a high speed CPU. We found that there was some amygdafa neurons related to place; and those place-related cells were controlled by proximal cues Moreover, those neurons responded cleady in familiar environment only. The result suggests the rote of amygdala In spatial long-term memory retention, acting as a reference helping hippocampus in receiving novel informathn in new environments.
Key words: amygdala, hippocampus, place cell
This work was supported by the Asian Core Program, JSPS, Japan and the Vietnam Ministry of Science and Technotogy (2643/QB-BKHCN).
