Tgp chi Klioa hgc 2012:21a 139-143 Truamg Dgi /igc Cdn Tho
KHAO SAT THANH PHAN HOA HOC VA HOAT TINH KHANG VI SINH VAT CUA TINH DAU GlfNG (ZINGIBER
OFFICINALE ROSCOE) VA TIIN(H DAU TIEU {PIPER NIGRUM L.)
Nguyen Thanh Hue. Trinh Minh Khang, Nguyin Tdn Hoang San vd Nguyin Thi Bich Thuyin ABSTRACT
This paper presents the results of study on chemical composition and some of anti- microorganism activity of ginger essential oil (Zingiber officinale Roscoe) and black pepper essential oil (Piper nigrum L.) in Me Kong Delta. The volatile oil obtained by the steam distillation without and with the assistance of microwave. The chemical composition of volatile oil was analyzed by GC—MS. Antimicroorganism was tested by MIC method.
Keywords: essential oil, ginger, pepper
Title: Study on chemical composition and some of anti-microorganism activity of ginger essential oil (Zingiber officinale Roscoe) and black pepper essential oil (Piper nigrum L.)
TOM TAT
Bdi bdo trinh bay ket qud khdo sdt thanh phdn hoa hoc vd hogt tinh khdng vi sinh vdt cua tinh ddu cu gimg vd tinh ddu hgt tieu a Dong Bdng Song Ciru Long. Tinh ddu thu dugc bdng phucmg phdp chimg cdt loi cudn hcri nude co dien vd chung cdt loi cudn hai nude CO hS tro ciia vi song. Xdc dinh thanh phdn hod hgc bdng GC-MS. Xdc dinh hogt tinh khdng vi sinh vgt bdng phuang phdp MIC. Ket qud cho thdy thanh phdn chinh cua tinh ddu tiiu Id trans-Caiyophyllene (27%), thanh phdn chinh cita tinh ddu girng Id Zingiberene (17%). Cd hai logi tinh ddu khdng tdt tren cdc vi sinh vdt thu nghiem.
Tir khda: tinh ddu, gu-ng, tiiu 1 DAT VAN DE
Ngoii chiic nang l i loai gia vi phd biln Uong cic mdn an. Gimg v i tjeu cdn la ciy thao dupe liu ddi trong y hpc din gian. X i hdi phat triln. cac chi phim tir gimg va tieu ngay cang phong phu han, tu bii thudc din gian cd dien, cho den thuc phim chiic ning, dupe phim v i my phim.
2 NGUYEN LIEU VA PHlTONG PHAP 2.1 Nguyen lieu
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M i u nguyen lieu dupe dinh danh tai Khoa Khoa hpc tu nhien- Dgi hpc Can Tha.
Tgp chi Khoa hoc 2012 2 la 139-143 Truong DgihgcCdnTha
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2.3 Phan tich thanh phan hoa hoc
Thanh phin hoa hpc dupe xac dinh bang phuang phip sic ky khi ghep khdi phd GC-MS, thuc hien Uen may Agilent Technologies 6890N (USA), cdt HP5-MS (dai 30 m; dudng kinh Uong 0,25 mm; ldp phim day 0,25 \xm) lien hop vdi miy khdi phd Agilent Technologies 5973 inert (USA), khi mang Heli (0,9 ml/phiit).
Chuong trinh nhiet dp: 40 °C (giir 2 phut), ting 3°C/phut cho d i n 200''C (giii 2 phut), tang 20°C/phiit din 250°C (giir 10 phut). Nhiet dp inlet: 250°C, nhiet dp MSD: 350°C. Thu vien phd NIST-MS search 2.0a-2002.
2.4 Hoat tinb khang vi sinh vat
Hogt tinh khing v i sinh vit cua tinh diu gimg va tieu dupe thu bang phuang phip MIC-ndng dp lic che tdi thieu ddi vdi mdt sd vi sinh vit thOr nghiem.
3 K £ T QUA VA THAO LUAN 3.1 Thanh phan hoa hoc
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Tinh dau hgt tieu dgng Idng, nhe ban nude, mau Uing, miii tham dac tnmg cua tieu.
Bang 1: Thanh phan hoa h9c cua tiah dau girng STT
1 2 3 4 5 6 7 8 9 10 U 12 13 14 IS 16 17 18
Thinh phan a-Pinene Camphene
6-Methyl-5-hepten-2-one
^Pinene
>ff-Myrcene a-Pheltandrene
^3-Carene l,8<;ineole (Eucalyptol) trans-Ociment Q^Terpinolene Linalool Bomeol 4-Terpineol or-Terpinol Acetic acid.octyl ester
®-Citral Geraniol B-C'iVra
Co diSn 1.39 4.04
-
0.34 1.76 0.16 5.92 0.12 0.33 0.78 0.79 0.08 0.75 0.16 4.84 5.28 7.08Him liKme (%) Vi song 0.62 1.94 0.07 0.33 1.05 0.12 0.34 4.97 0.12 0.29 1.16 0.99 0.11 1.08 0.20 5.45 8.50 9.41
T(^ chi Khoa hgc 2012:21a 139-143 Trudng Dgi hgc Cdn Tho 19 Bomyl acetate
20 or-Terpinene 21 Citronellyl acetate 22 Neryl acetate
Geranyl acetate QT-Copaene 25 >S-Elemene 26 rrotts-Caryophyllene
;^EIemene
^Famesene a-Curcumene a-Gurjunene CE-Selinene 32 Zingiberene 33 cr-Farnesene 34 >S-Sesquiphellandrene (CAS) 35 rr<3tt5-;*-Bisabolene
Elemol
Germacrene B (CAS) 38 Nerolidol 39 ^Eudesmol Ghi chu. - khongxac djnh
Bdng 2: Th^nh ph^n hda hoc cua tinb diu 23
24
27 28 29 30 31
36 37
0.17 0.11 0.68 O.ll
-
0.56 0.69 0.15 0.29 0.17 2.60 0.23 0.57 16.75 11.50 6.26 0.30 0.81 0.21 0.78 0.370.20
-
1.040.15 28.23
-
0.27 0.19 0.13 2.52 0.47 9.05 7.93 4.35 0.22 0.79 0.15 0.76 0.43STT Thinh phin C6 diln
Ham luffng (%) Vi song 1
2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 24
a-Thujene or-Pinene Camphene Sabinene yff-Pinene /^Myrcene CK-Phellandrene i5.3-Carene
£tr-Terpinene p-Cymene
^Phellandrene Limonene (Z)-,S-Ocimene
;*.Terpinene transSshinene hydrate PhenoI-2-methoxy-(CAS) or-Terpinolene c/5-Sabinene hydrate Linalool Alloocimene .^-Terpinol a-Terpinol a-Terpinene
1.29 3.97 0.13 7.94 7.10 2.01 2.78 14.03 0.81 0.67 1.60 15.47
0.15 0.91 0,11 0.30 0.95 0.55 0.24 0.57 0.11 0.29
1.55 5.33 0.12 10.50 7.89 2.22 2.26 10.93
1.00 0.44 1.83 16.99 0.16 1.23 0.19 0.78 0.14 0.69 0.34 0.11
Tgp chi Khoa hgc 2012:21a 139-143 Truong Dgi hgc Cdn Tha
25 (5-Elemene 26 Neryl acetate 27 ot-Copaene 28 ;5-EIemene 2 9 cis-Caryophyllene 3 0 rra/t5-C3ryophyllene 31 ;^EIemene 32 or-Gurjunene 34 a - G u a i e n e 35 a-Caryophyllene 36 ^ F a m e s e n e 37 Germacrene D 38 ^ S e l i n e n e 39 a-Selinene 40 t^Guaiene 41 ^ B i s a b o l e n e 42 a-Panasinsen 43 Caryophyllene oxide Ghi chu - Utang xdc dinh 3 . 2 C h i s o b o a ly
Bang 3 : Chi so boa ly ctia tinh dau girng v^ tinh dau tieu 1.37 0.19 1.25 0.29 2 7 . 0 3 0.09 0.55 1.45 0.12 0.28 1.72 1.31 0.12 0.63 0.14 0.14
0.49 0.06 0.19 0.88 2 5 . 8 5
-
0.08 0.51
-
1.48 0.07 0.43 1.64 1.16 0.06 0.54 0.09 0.31
. C h i so hoa Iv T i n h d a u girng Ty trpng 0.97 : h i so acid (lA) 0.80
; h i so savon hoa (IS) 10.09 : h i s6 ester (IE) 9.28 3 3 H o ^ t t i n h k h a n g v i s i n l i v a t
Bang 4: Hoat tinh khang vi sinh vat ciia tinh d^u gimg Vi k h u a n t h i r n g h i e m
Bacillus subtilis A T C C 6633 Enterococctts feacalis A T C C 29212 Pseudomonas aeruginosa A T C C 27853 Slaphvlocociis aureus A T C C 25923 Samonelta typhi T y 2
Candida albicans ATCC 10231
N o n g d o t i n h d a 6.25 6.25 50 6.25 6.25 0.06
T i n h d a u t i e u 0.86
0.67 14.57 13.89
u ire c h e ( m g / m l )
Bang 5: Ho^t tinh khang vi sinh v^t ciia tinh dau tiSu Vi k h u a n thir n g h i e m
Bacillus subtilis ATCC 6633 Enterococcus feacalis A T C C 29212 Pseudomonas aeruginosa A T C C 27853 Staphylococus aureus A T C C 25923 Samonella typhi Ty2
Candida albicans A T C C 10231
N 6 n g d o t i n h d i u u c c h ^ (mg/ml>
6.25 6.25 50 6.25 6.25 6.25
Tgp chi Khoa hgc 2012.21a 139-143 Truong Dai hgc Cdn Tha
4 KfeTLU^N
Thanh phan chinh ciia tinh diu gimg thu ducrc bang hju phuang phip chung cat la zingiberene (17%), ar-famesene (12%), ^sesquiphellandrene (6%), 1,8-cineole (6%). So vdi phuang phap chung cit bing ldi cudn hoi nude cd hd trp ciia vi sdng, thi phuang phap chung cit cd dien thdng thudng cd ham lupng cac ciu tii chinh cao hem.
Thinh phin chinh cua tinh diu tieu thu dupe bing hai phuong phap cit cd ham lupng tuang duang, /raw5-caryophyIIene (27%), limonene (15%), (5-3~carene (14%). Tuy nhien, chung cit bing Idi cudn hai nude cd hd Up cua vi sdng khdng thu dupe /ranj-caryophyllene.
C i hai tinh d i u khing tdt mdt sd vi sinh vat thii nghiem Candida albicans ATCC 10231 Bacillus subtilis ATCC 6633, Enterococcus feacalis ATCC 29212, Staphylococus aureus ATCC 25923.
TAI L l £ U THAM KHAO
Donell. D. W, Sutherlan. M. D, 1969, A re-examination of gingerol, shogaol, zingerone, the pungent principles of Ginger (Zingiber officinale Roscoe). Aust. J. Chem, 22, 1033- 1041.
GS-TS D§ TSt Loi, 1995. Nliung cay thu6c v a v i thudc ViSt Nam. NXB KHKT, 475-478.
HoMg Van Lini (2003), "Thanh ph3n hoa hoc ciia tiiih ddu cay Ho tieu P/per mgrw/n L. v& tirih dau cSy triu khong/*;/>«• 6er/e L. a Nghe An", Tgp chi Dugc hoc, 43(11), trang 15-17.
Masada.. Y, Inoue. T, Hashimoto. K, Fujioka. M, Shiraki. K, 1973. Studies on the pungent principlesof Ginger by GC-MS. VakugakiZasshi, 93.318-321.
Menon, A N, Padmakumari, K P, Jayalekshmy, A. (2003), "Essential Oil Composition of Four Major Cultivars of Black Pepper (Piper nigrum L.) IH", Journal OfEssentigl OH Research, 15(3), pp. 155-157.
Pham Hoang Ho, 1999. Cay co Viet Nam. Nha xuSt ban Tre, quyin 3,444-447.
V6 Van Chi, 1999. Tit diln cay thudc Viet Nam, NXB Yhoc.
