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Lampiran 2. Kromatogram hasil analisis beberapa fraksi ekstrak etil asetat biji dan umbi teratai
Biji Teratai : Fraksi 11
Keterangan : Senyawa dugaan (berdasarkan library: Wiley229.LIB) 2 =Nummularine (C29H38N4O5 )
6= Hexadecanoic acid (CAS) Palmitic acid (C16H32O2 )
8=4-Heptanone,2-methyl-(CAS)2-Methyl-4-heptanone (C8H16O ) 9=11,14-Eicosadienoic acid, methyl ester (C21H38O2 )
10=Phenol, 4-bromo-2-(1,2-dimethyl-3-methylenecyclo)( C15H18BrIO ) 16=δ-tocopherol (C27H46O2 )
Fraksi 10
Keterangan : Senyawa dugaan (berdasarkan library: Wiley229.LIB) 8 =1,2-Benzenedicarboxylic acid, diisoctyl ester (C24H38O4 )
Fraksi 9
Keterangan : Senyawa dugaan (berdasarkan library: Wiley229.LIB) 2 =1,2-Dimethyl 3-(3-Bromo-4-methoxy-2H-pyran-2-on-6-yl) indolizidinedicarboxylate (C18H14BrNO7 )
3= Diacetin (C17H12O5)
4= Benzenaminium,3-carboxy-N,N,N-trimethyl-,hydroxyide, inner salt (C10H13NO2)
8= Benzene,(1-bromoethyl)(CAS) 1-Phenylethyl bromide (C8H9Br) 11=3-(2,6-Dimethylphenyl)-1,2-benzisoxazole (C15H13NO)
Fraksi 8
Keterangan : Senyawa dugaan (berdasarkan library: Wiley229.LIB)
Fraksi 7
Keterangan : Senyawa dugaan (berdasarkan library: Wiley229.LIB) 1 =4-T-Butyl-2-isopropoxymethylenecyclohexanone(C14H24O2) Fraksi 5
Keterangan : Senyawa dugaan (berdasarkan library: Wiley229.LIB) 3 = Diacetin (C7H38O4)
5= 2-methyl-6-bete-d-ribofuranoylimidazol(1,2)pyrimidin5(6H)-one (C12H15N3O5)
6= Di-(2-ethylhexyl)ester of Adipic acid (C22H42O4)
Umbi Teratai : Fraksi 10
Keterangan : Senyawa dugaan (berdasarkan library: Wiley229.LIB) 5= Phenol, 4-bromo-2-(1,2-dimethyl-3-methylenecyclo)
6= Acetic acid, cyano
9=1,2-Benzenedicarboxylic acid, bis(2-ethylhexyl)ester
Fraksi 9
Keterangan : Senyawa dugaan (berdasarkan library: Wiley229.LIB) 3=3-Octadecene 8=1-Hexadecene(CAS) Cetene 12=1-Pentadecanol 15=1-Nonadecene 21=2,6,10,14,18,22-Tetracosahexaene,2,6,10,15,19,23-hexamethyl 22=Dodecane,2,6,22-trimethyl
Fraksi 8
Keterangan : Senyawa dugaan (berdasarkan library: Wiley229.LIB) 1=p-Terphenyl,2,4,4”,6-tetachloro
4=Hexadecanoic acid(CAS) Palmitic acid 5=9-Hexadecenoic acid
Fraksi 6
Keterangan : Senyawa dugaan (berdasarkan library: Wiley229.LIB) 6=Diisoamylene
8=2-methyl-6-beta-d-ribofuranosylimidazo[1,2-c] pyrimidin-5(6H)-one 9= Eicosyl acetate
Lampiran 3. Hasil analisis ragam penelitian pendahuluan Keterangan : Perlakuan 1 = kontrol Perlakuan 2 = 10 MIC Perlakuan 3 = 20 MIC Perlakuan 4 = 30 MIC
a. Total mikroba mukosa sekum
b. Total mikroba isi sekum
c. Total E.coli mukosa sekum
ANOVA TPCMUKO 1.896 3 .632 2.462 .137 2.053 8 .257 3.949 11 Between Groups Within Groups Total Sum of
Squares df Mean Square F Sig.
ANOVA TPCCECAL .035 3 .012 .058 .980 1.630 8 .204 1.665 11 Between Groups Within Groups Total Sum of
Squares df Mean Square F Sig.
ANOVA COLIMUKO 8.554 3 2.851 3.919 .062 5.094 7 .728 13.648 10 Between Groups Within Groups Total Sum of
Squares df Mean Square F Sig.
COLIMUKO Duncan a,b 3 4.909722 2 5.821034 5.821034 3 6.868144 3 7.003613 .257 .167 PERLAKUA tiga dua empat satu Sig. N 1 2
Subset for alpha = .05
Means for groups in homogeneous subsets are displayed. Uses Harmonic Mean Sample Size = 2.667. a.
The group sizes are unequal. The harmonic mean of the group sizes is used. Type I error levels are not guaranteed.
d. Total E.coli isi sekum
e. Total BAL aerob mukosa sekum
f. Total BAL aerob isi sekum
ANOVA COLICECA 4.975 3 1.658 7.877 .009 1.684 8 .211 6.659 11 Between Groups Within Groups Total Sum of
Squares df Mean Square F Sig.
COLICECA Duncan a 3 5.554333 3 6.444633 3 6.838333 3 7.307267 1.000 .058 PERLAKUA tiga empat dua satu Sig. N 1 2
Subset for alpha = .05
Means for groups in homogeneous subsets are displayed. Uses Harmonic Mean Sample Size = 3.000. a. ANOVA BALMUKOS 1.268 3 .423 2.126 .185 1.392 7 .199 2.661 10 Between Groups Within Groups Total Sum of
Squares df Mean Square F Sig.
ANOVA BALCECAL .473 3 .158 .390 .763 3.233 8 .404 3.706 11 Between Groups Within Groups Total Sum of
Lampiran 4. Hasil analisis ragam total ransum dan kenaikkan berat badan tikus percobaan
Keterangan:
Perlakuan 1 = kontrol Perlakuan 2 = biji teratai
Perlakuan 3 = ekstrak biji teratai Perlakuan 4 = FOS
a. Total ransum tikus percobaan yang sehat
b. Kenaikkan berat badan tikus percobaan yang sehat
ANOVA KONSNOR 1008.406 3 336.135 1.378 .318 1951.013 8 243.877 2959.420 11 Between Groups Within Groups Total Sum of
Squares df Mean Square F Sig.
ANOVA BBNOR 250.000 3 83.333 3.731 .061 178.667 8 22.333 428.667 11 Between Groups Within Groups Total Sum of
Squares df Mean Square F Sig.
BBNOR Duncana 3 29.666667 3 38.666667 3 39.333333 3 41.666667 1.000 .477 PERLAKUA tiga empat dua satu Sig. N 1 2
Subset for alpha = .05
Means for groups in homogeneous subsets are displayed. Uses Harmonic Mean Sample Size = 3.000. a.
f. Efisiensi ransum pada kelompok tikus sehat
d. Total ransum tikus percobaan yang diintervensi EPEC K1.1
e. Kenaikan berat badan tikus percobaan yang diintervensi EPEC K1.1
ANOVA EFISNOR 33.945 3 11.315 9.432 .005 9.597 8 1.200 43.542 11 Between Groups Within Groups Total Sum of
Squares df Mean Square F Sig.
EFISNOR Duncana 3 11.497480 3 14.072474 3 14.795194 3 16.114784 1.000 .060 PERLAKUA tiga dua empat satu Sig. N 1 2
Subset for alpha = .05
Means for groups in homogeneous subsets are displayed Uses Harmonic Mean Sample Size = 3.000. a. ANOVA KONSCOL 701.494 3 233.831 1.529 .280 1223.796 8 152.974 1925.289 11 Between Groups Within Groups Total Sum of
Squares df Mean Square F Sig.
ANOVA BBCOL 519.000 3 173.000 5.581 .023 248.000 8 31.000 767.000 11 Between Groups Within Groups Total Sum of
f. Efisiensi ransum pada kelompok tikus percobaan yang diintervensi EPEC K1.1 BBCOL Duncana 3 31.666667 3 36.666667 36.666667 3 46.666667 3 47.000000 .303 .061 PERLAKUA tiga satu empat dua Sig. N 1 2
Subset for alpha = .05
Means for groups in homogeneous subsets are displayed. Uses Harmonic Mean Sample Size = 3.000. a. ANOVA EFISCOL 52.435 3 17.478 6.861 .013 20.380 8 2.547 72.815 11 Between Groups Within Groups Total Sum of
Squares df Mean Square F Sig.
EFISCOL Duncana 3 12.156035 3 13.571030 3 16.910595 3 16.934812 .309 .986 PERLAKUA tiga satu empat dua Sig. N 1 2
Subset for alpha = .05
Means for groups in homogeneous subsets are displayed. Uses Harmonic Mean Sample Size = 3.000. a.
Lampiran 5. Hasil analisis ragam total mikroba pada sekum tikus percobaan Keterangan:
Perlakuan 1 = kontrol Perlakuan 2 = biji teratai
Perlakuan 3 = ekstrak biji teratai Perlakuan 4 = FOS
a. Total mikroba isi sekum-kelompok tikus sehat-2 minggu perlakuan ransum
b. Total mikroba isi sekum- kelompok tikus sehat- 3 minggu perlakuan ransum
c. Total mikroba isi sekum-kelompok tikus sehat-4 minggu perlakuan ransum ANOVA TPS2CS 2.315 3 .772 3.300 .079 1.871 8 .234 4.185 11 Between Groups Within Groups Total Sum of
Squares df Mean Square F Sig.
TPS2CS Duncana 3 8.135022 3 8.221415 3 8.425951 8.425951 3 9.245249 .500 .072 PERLAKUA empat tiga satu dua Sig. N 1 2
Subset for alpha = .05
Means for groups in homogeneous subsets are displayed. Uses Harmonic Mean Sample Size = 3.000. a. ANOVA TPC3CS .725 3 .242 1.789 .227 1.080 8 .135 1.805 11 Between Groups Within Groups Total Sum of
Squares df Mean Square F Sig.
ANOVA TPC4CS .609 3 .203 1.310 .337 1.240 8 .155 1.849 11 Between Groups Within Groups Total Sum of
d. Total mikroba isi sekum-kelompok tikus diintervensi EPEC K1.1-3 minggu perlakuan ransum
e. Total mikroba isi sekum- kelompok tikus diintervensi EPEC K1.1- 4 minggu perlakuan ransum
f. Total mikroba mukosa sekum-kelompok tikus sehat- 2 minggu perlakuan ransum ANOVA TPC3CC .637 3 .212 1.580 .269 1.074 8 .134 1.711 11 Between Groups Within Groups Total Sum of
Squares df Mean Square F Sig.
ANOVA TPC4CC 3.995 3 1.332 2.057 .184 5.178 8 .647 9.173 11 Between Groups Within Groups Total Sum of
Squares df Mean Square F Sig.
ANOVA TPC2M 4.672 3 1.557 6.244 .017 1.995 8 .249 6.668 11 Between Groups Within Groups Total Sum of
Squares df Mean Square F Sig.
TPC2M Duncana 3 4.568844 3 4.923103 3 5.883765 3 6.042958 .410 .706 PERLAKUA