V. KESIMPULAN
5. Hasil alignment sekuen DNA ikan nila putih dan ikan nila hitam dar
Nila hitam 3 f.gnu 1 CCCCCGATTGACCCC-TTCGTCATTATTGGCCAAATCGCATCTTTCCTCTACTTCTCCCT 59 Nila putih 7 f.gnu 1 GCCCCGGTTGACCCCCTTCGTCATTATTGGCCAAATTGCATCTTTCCTCTACTTCTTCCT 60
CCCCG TTGACCCC TTCGTCATTATTGGCCAAAT GCATCTTTCCTCTACTTCT CCT
CCCCG TTGACCCC TTCGTCATTATTGGCCAAAT GCATCTTTCCTCTACTTCT CCT
Nila hitam 3 f.gnu 60 CTTCCTCGTTTTCGCCCCCATCACCGGCTGGCTAGAAAACAAAATCCTTGAATGATACTG 119 Nila putih 7 f.gnu 61 CTTCCTCGTTCTCGCCCCTATTACCGGCTGGCTAGAAAACAAAATCCTTGAATGACACTG 120
CTTCCTCGTT TCGCCCCAT ACCGGCTGGCTAGAAAACAAAATCCTTGAATGA ACTG
CTTCCTCGTT TCGCCCCAT ACCGGCTGGCTAGAAAACAAAATCCTTGAATGA ACTG Nila hitam 3 f.gnu 120 CACTAGTAGCTCAGCACCAGAGCGCCGGTCTTGTAAACCGGATGTCGAAGGTTAAAGTCC179 Nila putih 7 f.gnu 121 CACTAGTAGCTCAGCACCAGAGCGCCGGTCTTGTAAACCGGATGTCGAAGGTTAAAGTCC180
CACTAGTAGCTCAGCACCAGAGCGCCGGTCTTGTAAACCGGATGTCGAAGGTTAAAGTCC CACTAGTAGCTCAGCACCAGAGCGCCGGTCTTGTAAACCGGATGTCGAAGGTTAAAGTCC
Nila hitam 3 f.gnu 180 TTCCTACTGCTTCAAAGAAAAGGGATTTTAACCCCTACCCCTAACTCCCAAAGCTAGGAT 239 Nila putih 7 f.gnu 181 TTCCTACTGCTTCAAAGAAAAGGGATTTTAACCCTTACCCCTAACTCCCAAAGCTAGGAT 240
TTCCTACTGCTTCAAAGAAAAGGGATTTTAACCCTACCCCTAACTCCCAAAGCTAGGAT TTCCTACTGCTTCAAAGAAAAGGGATTTTAACCCTACCCCTAACTCCCAAAGCTAGGAT
Nila hitam 3 f.gnu 240 TCTAACTTAAACTATTCTTTGCCGAGCTCTGCCTTCATGCAAAATACAATACATATATGT 299 Nila putih 7 f.gnu 241 TCTAATTTAAACTATTCTTTGCCGAGCTCTGCCTTCATGCAAA-TACAATGCATATATGT 299
TCTAATTAAACTATTCTTTGCCGAGCTCTGCCTTCATGCAAATACAAT CATATATGT TCTAATTAAACTATTCTTTGCCGAGCTCTGCCTTCATGCAAATACAAT CATATATGT
Nila hitam 3 f.gnu 300 ATTATCACCATTATTTTATTTCAAACATATCCTATATATAAATACATACAACTCTTAAAA359 Nila putih 7 f.gnu 300 ATTATCACCATTATTTTATATCAAACATATCCTATATATAAATACATACTTTT-TTAAAA358
ATTATCACCATTATTTTAT TCAAACATATCCTATATATAAATACATAC T TTAAAA ATTATCACCATTATTTTAT TCAAACATATCCTATATATAAATACATAC T TTAAAA
Nila hitam 3 f.gnu 360 AACATACACTGTTTTCCCACATATTTGTCATCAACATCTATAACTAAGAAGAACATAAAC 419 Nila putih 7 f.gnu 359 GACATCCACTGCTCCCCCACATATTTGCCAACAACATTTACAACTAAGAGAAACATAAAC 418
ACAT CACTG T CCCACATATTTG CA CAACAT TA AACTAAGA AACATAAAC
ACAT CACTG T CCCACATATTTG CA CAACAT TA AACTAAGA AACATAAAC
Nila hitam 3 f.gnu 420 CAATAAATGAAATATTCCAATAACGATTAAATACCACTGAACGATAGTTTAAGACCGAAC 479 Nila putih 7 f.gnu 419 CAGTAAATGGAACTTTCCAAAAACATTTCAAAACCACTAAACGACAGTTTAAGACCGAAC 478
CA TAAATG AA TTCCAA AAC TTAA ACCACT AACGA AGTTTAAGACCGAAC CA TAAATG AA TTCCAA AAC TTAA ACCACT AACGA AGTTTAAGACCGAAC
Nila hitam 3 f.gnu 480 ACAACTCTCATACAGTTAAGATATACCAAGTACCCAACATCCTATACTTCTAAATTATTT539 Nila putih 7 f.gnu 479 ACAACTCTCATAC-GTTAAGATATACCAAGTACCCACCATCCTATACTTCCGAATTATTT537
ACAACTCTCATAC GTTAAGATATACCAAGTACCCA CATCCTATACTTC AATTATTT
ACAACTCTCATAC GTTAAGATATACCAAGTACCCA CATCCTATACTTC AATTATTT
Nila hitam 3 f.gnu 540 AATGTAGTAAGAGCCCACCATCAGTTGATTCCTATATGTTAACGGTTCTTGAAGGTCAAG 599 Nila putih 7 f.gnu 538 AATGTAGTAAGAGCCCACCATCAGTTGATTTCTCAATGTTAACGGTTCTTGAAGGTCAAG 597
AATGTAGTAAGAGCCCACCATCAGTTGATTCT ATGTTAACGGTTCTTGAAGGTCAAG
AATGTAGTAAGAGCCCACCATCAGTTGATTCT ATGTTAACGGTTCTTGAAGGTCAAG Nila hitam 3 f.gnu 600 GACAATTATTTGTGGGGGTTTCACTAATTGAATTATTCCTGGCATCTGGTTCCTATTTCA 659 Nila putih 7 f.gnu 598 GACAGTTATTCGTGGGGGTTTCACTAATTGAATTATTCCTGGCATCTGGTTCCTATTTCA 657
GACA TTATTGTGGGGGTTTCACTAATTGAATTATTCCTGGCATCTGGTTCCTATTTCA
GACA TTATTGTGGGGGTTTCACTAATTGAATTATTCCTGGCATCTGGTTCCTATTTCA
Nila hitam 3 f.gnu 660 GGTCCAATAATTGTTATAATTCCCCATTCTTTCATCGACGCTTGCATAAGTTAATGGTGT 719 Nila putih 7 f.gnu 658 GGTCCAATAATTGTTATAATTCCCCATTCTTTCATCGACGCTTGCATAAGTTAATGGTGT 717
GGTCCAATAATTGTTATAATTCCCCATTCTTTCATCGACGCTTGCATAAGTTAATGGTGT
GGTCCAATAATTGTTATAATTCCCCATTCTTTCATCGACGCTTGCATAAGTTAATGGTGT
Nila hitam 3 f.gnu 720 CAATACATACTCCTCATTACCCAACATGCCGGGCGTTCTTTCCAGAGGATAGGGGGTTTC 779 Nila putih 7 f.gnu 718 TAATACATACTCCTCGTTACCCACCATGCCGGGCGTTCTTTCCAGGGTGTGGGGGGTT-C 776
AATACATACTCCTC TTACCCA CATGCCGGGCGTTCTTTCCAG G T GGGGGTTC
AATACATACTCCTC TTACCCA CATGCCGGGCGTTCTTTCCAG G T GGGGGTTC
Nila hitam 3 f.gnu 780 TCTTTTTTTTTTTCCTTTCATTTGGCATCTCAGAGTGCATACAGAAAT-GACAGACAAGG838 Nila putih 7 f.gnu 777 TCTTTTTTTTTTTCCTTTCACTTGACATTTCAGAGTGCATACAGAAAAAGACAGACAAGG836
TCTTTTTTTTTTTCCTTTCA TTG CAT TCAGAGTGCATACAGAAA GACAGACAAGG
TCTTTTTTTTTTTCCTTTCA TTG CAT TCAGAGTGCATACAGAAA GACAGACAAGG Nila hitam 3 f.gnu 839 TTGAACATTTTCCTTGCTTGGAGGTAAATAGTATGAATGATAGA-AGACATTGATA-GAA896 Nila putih 7 f.gnu 837 TTGAACATTTTCCTTGCTTGAACGGAAATAGTATGAATGGTGGTTAGATATTATTAAGAA896
TTGAACATTTTCCTTGCTTG A G AAATAGTATGAATG T G AGA ATT TA GAA
TTGAACATTTTCCTTGCTTG A G AAATAGTATGAATG T G AGA ATT TA GAA
Nila hitam 3 f.gnu 897 GTATTGCATAACTG-ATATCTAGAGCATAAAGTTCAATCAAATATTTCAATTTTCCTCCA 955 Nila putih 7 f.gnu 897 GAATTGCATAACTGGATATCTAGAGCATAAAGTTTAATCAAA-ATTTTAATTTTC-TCCT 954
G ATTGCATAACTG ATATCTAGAGCATAAAGTTAATCAAA ATTTAATTTTC TCC
G ATTGCATAACTG ATATCTAGAGCATAAAGTTAATCAAA ATTTAATTTTC TCC
Nila hitam 3 f.gnu 956 AATTTTCCTATTATTC-TTCGGTTTTTTCGCGCG-TAAACCCCCCCT-ACCCCCCCAAAA1012 Nila putih 7 f.gnu 955 AATTTTTCTATTAACCCTTCGGTTTTTGCGCGCGGTAAACCCCCCCTTACCCCCCCAAAA1014
AATTTTCTATTA C TTCGGTTTTT CGCGCG TAAACCCCCCCT ACCCCCCCAAAA AATTTTCTATTA C TTCGGTTTTT CGCGCG TAAACCCCCCCT ACCCCCCCAAAA
Nila hitam 3 f.gnu 1013 -CTCC-AAAGATC-CTAAA-ACTCCTGCAAACCCCCC--GGAAACAGGAAAAGCTCTTA- 1065 Nila putih 7 f.gnu 1015 ACTCCTAAAAATCTCTAAATATTCCTGCAAACCCCCCCGGAAAACGGGAAAAGCTTCTAG 1074
CTCCAAA ATC CTAAA ATCCTGCAAACCCCCC G AAAC GGAAAAGCT TA
CTCCAAA ATC CTAAA ATCCTGCAAACCCCCC G AAAC GGAAAAGCT TA
Nila hitam 3 f.gnu 1066 AATTGACTTTTAGC-GCTTTAGTTTAGGCAGGTTAAATA-ACTTAAGTGGTGGTAATGGC 1123 Nila putih 7 f.gnu 1075 AAGTAACTTTCACCCGCTTTAATAATTGCATAAAAAATTTACTTAAGTGGGGGAAATCCA 1134
AA T ACTTTA C GCTTTA T GCA AAAT ACTTAAGTGG GG AAT
AA T ACTTTA C GCTTTA T GCA AAAT ACTTAAGTGG GG AAT
Nila hitam 3 f.gnu 1124 AGATATTATCATGGCCCGGGCCATA-ATTCCAAGTGGGGGTATTTAAATTTTT--- 1175 Nila putih 7 f.gnu 1135 AGAACCTACAAAGGCACGAGTCATGTATCCCAAGTGGGTGGGTAAAAAATTAATTAAAAA1194
AGA TA A GGC CG GCAT AT CCAAGTGGG G T AAATT
AGA TA A GGC CG GCAT AT CCAAGTGGG G T AAATT
Nila hitam 3 f.gnu 1175 --- 1175
Lampiran 6. Hasilalignmentsekuen DNA ikan nila putih dan ikan nila hitam dari arah reverse
Nila hitam 3 r.gnu 1 GGTTTTTTAAACCGGAATTGGGAAGGTTAAAGTCCTTCCTTATTGGCTTCAAAGGAAAAG 60 Nila putih 7 r.gnu 1 ---AATCCCTTCCCTAACCCTTTCAAAAAAAAAA31
AA CCTTCC TA TTCAAA AAAA AA CCTTCC TA TTCAAA AAAA
Nila hitam 3 r.gnu 61 GGATTTTTAACCCCTA-CCCCTTAATTCCCCAAAGCT-AGGATTCTTAACTT-AAACTAT 117 Nila putih 7 r.gnu 32 GGAATTTTAACCCTTAACCCCTTAATTCCCAAAAGGTTAGGAATTTTAATTTTAAATTAT 91
GGA TTTTAACCC TA CCCCTTAATTCCC AAAG T AGGA T TTAA TT AAA TAT
GGA TTTTAACCC TA CCCCTTAATTCCC AAAG T AGGA T TTAA TT AAA TAT
Nila hitam 3 r.gnu 118 TTTTTGGCCGAG-TTTTGCCTC-CATGCAAAATACAATACA-ATTATGTATTAT---CCC 171 Nila putih 7 r.gnu 92 TTTTTTGCCGAGGTTTTGCCTTTCATGCAAATACCAATGCCTAATATGTTTTTTTCCCCC 151
TTTTT GCCGAG TTTTGCCT CATGCAAA CAAT C A TATGT TT T CCC
TTTTT GCCGAG TTTTGCCT CATGCAAA CAAT C A TATGT TT T CCC
Nila hitam 3 r.gnu 172 CATTATTTTATTTCAAACATATCCTATATATAAA-TACATACAACTCTTAAAAAACATAC 230 Nila putih 7 r.gnu 152 TTTTTTTTTTTTTCCAACATATCCCTAATATAAAATACATACTTTT-TTAAAAGACATCC 210
TT TTTT TTTC AACATATCC ATATAAA TACATAC T TTAAAA ACAT C
TT TTTT TTTC AACATATCC ATATAAA TACATAC T TTAAAA ACAT C
Nila hitam 3 r.gnu 231 ACTGTTTTCCC-ACATATTTGTCATCAACATCTATAACTTAGAAGAACATAAACCCATAA 289 Nila putih 7 r.gnu 211 ACTGGTTCCCCCACATATTTGCCAACAACATTTACAACTAAGAGAAACATAAACCAGTAA 270
ACTG TT CCC ACATATTTG CA CAACAT TA AACT AGA AACATAAACC TAA ACTG TT CCC ACATATTTG CA CAACAT TA AACT AGA AACATAAACC TAA
Nila hitam 3 r.gnu 290 ATGAAATATTCCAATAACGATTAAATACCACTGAACGATAGTTTAAGACCGAACACAACT 349 Nila putih 7 r.gnu 271 ATGGAACTTTCCAAAAACATTTCAAAACCACTAAACGACAGTTTAAGACCGAACACAACT 330
ATG AA TTCCAA AAC TT AA ACCACT AACGA AGTTTAAGACCGAACACAACT
ATG AA TTCCAA AAC TT AA ACCACT AACGA AGTTTAAGACCGAACACAACT
Nila hitam 3 r.gnu 350 CTCATACAGTTAAGATATACCAAGTACCCAACATCCTATACTTCTAAATTATTTAATGTA 409 Nila putih 7 r.gnu 331 CTCATAC-GTTAAGATATACCAAGTACCCACCATCCTATACTTCCGAATTATTTAATGTA 389
CTCATAC GTTAAGATATACCAAGTACCCA CATCCTATACTTC AATTATTTAATGTA
CTCATAC GTTAAGATATACCAAGTACCCA CATCCTATACTTC AATTATTTAATGTA
Nila hitam 3 r.gnu 410 GTAAGAGCCCACCATCAGTTGATTCCTATATGTTAACGGTTCTTGAAGGTCAAGGACAAT 469 Nila putih 7 r.gnu 390 GTAAGAGCCCACCATCAGTTGATTTCTCAATGTTAACGGTTCTTGAAGGTCAAGGACAGT 449
GTAAGAGCCCACCATCAGTTGATT CT ATGTTAACGGTTCTTGAAGGTCAAGGACA T
GTAAGAGCCCACCATCAGTTGATT CT ATGTTAACGGTTCTTGAAGGTCAAGGACA T
Nila hitam 3 r.gnu 470 TATTTGTGGGGGTTTCACTAATTGAATTATTCCTGGCATCTGGTTCCTATTTCAGGTCCA 529 Nila putih 7 r.gnu 450 TATTTGTGGGGGTTTCACTAATTGAATTATTCCTGGCATCTGGTTCCTATTTCAGGTCCA 509
TATTTGTGGGGGTTTCACTAATTGAATTATTCCTGGCATCTGGTTCCTATTTCAGGTCCA
TATTTGTGGGGGTTTCACTAATTGAATTATTCCTGGCATCTGGTTCCTATTTCAGGTCCA
Nila hitam 3 r.gnu 530 ATAATTGTTATAATTCCCCATTCTTTTATTGACGCTTGCATAAGTTAATGGTGTCAATAC 589 Nila putih 7 r.gnu 510 ATAATTGTTATAATTCCCCATTCTTTCATTGACGCTTGCATAAGTTAATGGTGTTAATAC 569
ATAATTGTTATAATTCCCCATTCTTT ATTGACGCTTGCATAAGTTAATGGTGT AATAC
ATAATTGTTATAATTCCCCATTCTTT ATTGACGCTTGCATAAGTTAATGGTGT AATAC
Nila hitam 3 r.gnu 590 ATACTCCTCATTACCCAACATGCCGGGCGTTCTTTCCAGAGGATAGGGGGTTTCTCTTTT 649 Nila putih 7 r.gnu 570 ATACTCCTCGTTACCCACCATGCCGGGCGTTCTTTCCAGGGTGTGGGGGGTT-CTCTTTT 628
ATACTCCTC TTACCCA CATGCCGGGCGTTCTTTCCAG G T GGGGGTT CTCTTTT
ATACTCCTC TTACCCA CATGCCGGGCGTTCTTTCCAG G T GGGGGTT CTCTTTT
Nila hitam 3 r.gnu 650 TTTTTTTCCTTTCATTTGGCATCTCAGAGTGCATACAGAAATGACAGACAAGGTTGAACA 709 Nila putih 7 r.gnu 629 TTTTTTTCCTTTCACTTGACATTTCAGAGTGCATACAGAAAAGACAGACAAGGTTGAACA 688
TTTTTTTCCTTTCA TTG CAT TCAGAGTGCATACAGAAA GACAGACAAGGTTGAACA
TTTTTTTCCTTTCA TTG CAT TCAGAGTGCATACAGAAA GACAGACAAGGTTGAACA
Nila hitam 3 r.gnu 710 TTTTCCTTGCTTGGAGGTAAATAGTATGAATGATAGA-AGACATTGATA-GAAGTATTGC 767 Nila putih 7 r.gnu 689 TTTTCCTTGCTTGAACGGAAATAGTATGAATGGTGGTTAGATATTATTAAGAAGAATTGC 748
TTTTCCTTGCTTG A G AAATAGTATGAATG T G AGA ATT TA GAAG ATTGC
TTTTCCTTGCTTG A G AAATAGTATGAATG T G AGA ATT TA GAAG ATTGC
Nila hitam 3 r.gnu 768 ATAACTGATATCTAGAGCATAAAGTTCAATCAAATATTTCAATTTTCTCCTAATTTTTCT 827 Nila putih 7 r.gnu 749 ATAACTGATATCTAGAGCATAAAGTTTAATCAAA-ATTTTAATTTTCTCCTAATTTTTCT 807
ATAACTGATATCTAGAGCATAAAGTT AATCAAA ATTT AATTTTCTCCTAATTTTTCT
ATAACTGATATCTAGAGCATAAAGTT AATCAAA ATTT AATTTTCTCCTAATTTTTCT
Nila hitam 3 r.gnu 828 ATTATTCTTCGGTTTTTTCGCGCGTAAACCCCCCCTACCCCCCCAAAACTCCTAAGATCT 887 Nila putih 7 r.gnu 808 ATTAACCTTCGGTTTTTGCGCGCGTAAACCCCCCCTACCCCCCCAAAACTCCTAAGATCT 867
ATTA CTTCGGTTTTT CGCGCGTAAACCCCCCCTACCCCCCCAAAACTCCTAAGATCT
ATTA CTTCGGTTTTT CGCGCGTAAACCCCCCCTACCCCCCCAAAACTCCTAAGATCT
Nila hitam 3 r.gnu 888 CTAATACTCCTGCAAACCCCCCGGAAACAGGAAAAGCTCTAGAAGTGACTTTTAGCGCTT 947 Nila putih 7 r.gnu 868 CTAATATTCCTGCAAACCCCCCGGAAACAGGAAAAGCTCTAGAAGTAACTTTCAGCGCTT 927
CTAATA TCCTGCAAACCCCCCGGAAACAGGAAAAGCTCTAGAAGT ACTTT AGCGCTT
CTAATA TCCTGCAAACCCCCCGGAAACAGGAAAAGCTCTAGAAGT ACTTT AGCGCTT
Nila hitam 3 r.gnu 948 TAGTTTATGCATGTTATATTACTTAATGTGTGTATATGCAGTATTATCAATGCACGGGCC 1007 Nila putih 7 r.gnu 928 TAATATATGCATAAAATATTACTTAATGTGTGTATATCTAGTACTATCAATGCACGAGTC 987
TA T TATGCAT ATATTACTTAATGTGTGTATAT AGTA TATCAATGCACG G C
TA T TATGCAT ATATTACTTAATGTGTGTATAT AGTA TATCAATGCACG G C
Nila hitam 3 r.gnu 1008 ATATATCCAATGTGTGTATATTATATTATTATAATATTGCACATGCTAGCGTAGCTTAAC 1067 Nila putih 7 r.gnu 988 ATGTATCCAATGTGTGTGTATTATATTATTATAATATTGCACATGCTAGCGTAGCTTAAC 1047
AT TATCCAATGTGTGT TATTATATTATTATAATATTGCACATGCTAGCGTAGCTTAAC
AT TATCCAATGTGTGT TATTATATTATTATAATATTGCACATGCTAGCGTAGCTTAAC
Nila hitam 3 r.gnu 1068 TAAAGCATAACACTGAAGATGTTAAGACGGACCCTAGAAAGGCCCCGTAAGCACAAAGGC 1127 Nila putih 7 r.gnu 1048 TAAAGCATAACACTGAAGATGTTAAGACGGACCCTAGAAAGGCCCCGTAAGCACAAAGGC 1107
TAAAGCATAACACTGAAGATGTTAAGACGGACCCTAGAAAGGCCCCGTAAGCACAAAGGC
TAAAGCATAACACTGAAGATGTTAAGACGGACCCTAGAAAGGCCCCGTAAGCACAAAGGC
Nila hitam 3 r.gnu 1128 TTGGTCCTGACTT-ACTTCAGTT-GAAACCC 1156 Nila putih 7 r.gnu 1108 TTGGTCCTGACTTTACTTCAGTTTGAAACCC 1138
TTGGTCCTGACTT ACTTCAGTT GAAACCC
Lampiran 7. Elektroforegram hasil PCR kombinasi primer yang tidak spesifik