KESIMPULAN - Colonization and proliferation of white Nile tilapia testicular cells transplanted

Marka molekuler ikan nila putih berhasil didesain dan dapat digunakan untuk membedakan sel testikular ikan nila putih di dalam tubuh ikan nila hitam. Selanjutnya sel testikular ikan nila putih berhasil terkolonisasi dan terproliferasi di dalam gonad ikan nila hitam triploid.

5.2 Saran

Uji fungsional gamet ikan nila hitam triploid perlu dilakukan untuk memperoleh induk ikan nila resipien yang dapat menghasilkan sperma dan telur ikan nila putih. Analisis kadar dan jenis hormon perlu dilakukan untuk mengkaji regulasi hormonal pada ikan nila triploid transplan.

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Lampiran 5. Hasilalignmentsekuen DNA ikan nila putih dan ikan nila hitam dari arahforward

Nila hitam 3 f.gnu 1 CCCCCGATTGACCCC-TTCGTCATTATTGGCCAAATCGCATCTTTCCTCTACTTCTCCCT 59 Nila putih 7 f.gnu 1 GCCCCGGTTGACCCCCTTCGTCATTATTGGCCAAATTGCATCTTTCCTCTACTTCTTCCT 60

CCCCG TTGACCCC TTCGTCATTATTGGCCAAAT GCATCTTTCCTCTACTTCT CCT

Nila hitam 3 f.gnu 60 CTTCCTCGTTTTCGCCCCCATCACCGGCTGGCTAGAAAACAAAATCCTTGAATGATACTG 119 Nila putih 7 f.gnu 61 CTTCCTCGTTCTCGCCCCTATTACCGGCTGGCTAGAAAACAAAATCCTTGAATGACACTG 120

CTTCCTCGTT TCGCCCCAT ACCGGCTGGCTAGAAAACAAAATCCTTGAATGA ACTG

CTTCCTCGTT TCGCCCCAT ACCGGCTGGCTAGAAAACAAAATCCTTGAATGA ACTG Nila hitam 3 f.gnu 120 CACTAGTAGCTCAGCACCAGAGCGCCGGTCTTGTAAACCGGATGTCGAAGGTTAAAGTCC179 Nila putih 7 f.gnu 121 CACTAGTAGCTCAGCACCAGAGCGCCGGTCTTGTAAACCGGATGTCGAAGGTTAAAGTCC180

CACTAGTAGCTCAGCACCAGAGCGCCGGTCTTGTAAACCGGATGTCGAAGGTTAAAGTCC CACTAGTAGCTCAGCACCAGAGCGCCGGTCTTGTAAACCGGATGTCGAAGGTTAAAGTCC

Nila hitam 3 f.gnu 180 TTCCTACTGCTTCAAAGAAAAGGGATTTTAACCCCTACCCCTAACTCCCAAAGCTAGGAT 239 Nila putih 7 f.gnu 181 TTCCTACTGCTTCAAAGAAAAGGGATTTTAACCCTTACCCCTAACTCCCAAAGCTAGGAT 240

TTCCTACTGCTTCAAAGAAAAGGGATTTTAACCCTACCCCTAACTCCCAAAGCTAGGAT TTCCTACTGCTTCAAAGAAAAGGGATTTTAACCCTACCCCTAACTCCCAAAGCTAGGAT

Nila hitam 3 f.gnu 240 TCTAACTTAAACTATTCTTTGCCGAGCTCTGCCTTCATGCAAAATACAATACATATATGT 299 Nila putih 7 f.gnu 241 TCTAATTTAAACTATTCTTTGCCGAGCTCTGCCTTCATGCAAA-TACAATGCATATATGT 299

TCTAATTAAACTATTCTTTGCCGAGCTCTGCCTTCATGCAAATACAAT CATATATGT TCTAATTAAACTATTCTTTGCCGAGCTCTGCCTTCATGCAAATACAAT CATATATGT

Nila hitam 3 f.gnu 300 ATTATCACCATTATTTTATTTCAAACATATCCTATATATAAATACATACAACTCTTAAAA359 Nila putih 7 f.gnu 300 ATTATCACCATTATTTTATATCAAACATATCCTATATATAAATACATACTTTT-TTAAAA358

ATTATCACCATTATTTTAT TCAAACATATCCTATATATAAATACATAC T TTAAAA ATTATCACCATTATTTTAT TCAAACATATCCTATATATAAATACATAC T TTAAAA

Nila hitam 3 f.gnu 360 AACATACACTGTTTTCCCACATATTTGTCATCAACATCTATAACTAAGAAGAACATAAAC 419 Nila putih 7 f.gnu 359 GACATCCACTGCTCCCCCACATATTTGCCAACAACATTTACAACTAAGAGAAACATAAAC 418

ACAT CACTG T CCCACATATTTG CA CAACAT TA AACTAAGA AACATAAAC

Nila hitam 3 f.gnu 420 CAATAAATGAAATATTCCAATAACGATTAAATACCACTGAACGATAGTTTAAGACCGAAC 479 Nila putih 7 f.gnu 419 CAGTAAATGGAACTTTCCAAAAACATTTCAAAACCACTAAACGACAGTTTAAGACCGAAC 478

CA TAAATG AA TTCCAA AAC TTAA ACCACT AACGA AGTTTAAGACCGAAC CA TAAATG AA TTCCAA AAC TTAA ACCACT AACGA AGTTTAAGACCGAAC

Nila hitam 3 f.gnu 480 ACAACTCTCATACAGTTAAGATATACCAAGTACCCAACATCCTATACTTCTAAATTATTT539 Nila putih 7 f.gnu 479 ACAACTCTCATAC-GTTAAGATATACCAAGTACCCACCATCCTATACTTCCGAATTATTT537

ACAACTCTCATAC GTTAAGATATACCAAGTACCCA CATCCTATACTTC AATTATTT

Nila hitam 3 f.gnu 540 AATGTAGTAAGAGCCCACCATCAGTTGATTCCTATATGTTAACGGTTCTTGAAGGTCAAG 599 Nila putih 7 f.gnu 538 AATGTAGTAAGAGCCCACCATCAGTTGATTTCTCAATGTTAACGGTTCTTGAAGGTCAAG 597

AATGTAGTAAGAGCCCACCATCAGTTGATTCT ATGTTAACGGTTCTTGAAGGTCAAG

AATGTAGTAAGAGCCCACCATCAGTTGATTCT ATGTTAACGGTTCTTGAAGGTCAAG Nila hitam 3 f.gnu 600 GACAATTATTTGTGGGGGTTTCACTAATTGAATTATTCCTGGCATCTGGTTCCTATTTCA 659 Nila putih 7 f.gnu 598 GACAGTTATTCGTGGGGGTTTCACTAATTGAATTATTCCTGGCATCTGGTTCCTATTTCA 657

GACA TTATTGTGGGGGTTTCACTAATTGAATTATTCCTGGCATCTGGTTCCTATTTCA

Nila hitam 3 f.gnu 660 GGTCCAATAATTGTTATAATTCCCCATTCTTTCATCGACGCTTGCATAAGTTAATGGTGT 719 Nila putih 7 f.gnu 658 GGTCCAATAATTGTTATAATTCCCCATTCTTTCATCGACGCTTGCATAAGTTAATGGTGT 717

GGTCCAATAATTGTTATAATTCCCCATTCTTTCATCGACGCTTGCATAAGTTAATGGTGT

Nila hitam 3 f.gnu 720 CAATACATACTCCTCATTACCCAACATGCCGGGCGTTCTTTCCAGAGGATAGGGGGTTTC 779 Nila putih 7 f.gnu 718 TAATACATACTCCTCGTTACCCACCATGCCGGGCGTTCTTTCCAGGGTGTGGGGGGTT-C 776

AATACATACTCCTC TTACCCA CATGCCGGGCGTTCTTTCCAG G T GGGGGTTC

Nila hitam 3 f.gnu 780 TCTTTTTTTTTTTCCTTTCATTTGGCATCTCAGAGTGCATACAGAAAT-GACAGACAAGG838 Nila putih 7 f.gnu 777 TCTTTTTTTTTTTCCTTTCACTTGACATTTCAGAGTGCATACAGAAAAAGACAGACAAGG836

TCTTTTTTTTTTTCCTTTCA TTG CAT TCAGAGTGCATACAGAAA GACAGACAAGG

TCTTTTTTTTTTTCCTTTCA TTG CAT TCAGAGTGCATACAGAAA GACAGACAAGG Nila hitam 3 f.gnu 839 TTGAACATTTTCCTTGCTTGGAGGTAAATAGTATGAATGATAGA-AGACATTGATA-GAA896 Nila putih 7 f.gnu 837 TTGAACATTTTCCTTGCTTGAACGGAAATAGTATGAATGGTGGTTAGATATTATTAAGAA896

TTGAACATTTTCCTTGCTTG A G AAATAGTATGAATG T G AGA ATT TA GAA

Nila hitam 3 f.gnu 897 GTATTGCATAACTG-ATATCTAGAGCATAAAGTTCAATCAAATATTTCAATTTTCCTCCA 955 Nila putih 7 f.gnu 897 GAATTGCATAACTGGATATCTAGAGCATAAAGTTTAATCAAA-ATTTTAATTTTC-TCCT 954

G ATTGCATAACTG ATATCTAGAGCATAAAGTTAATCAAA ATTTAATTTTC TCC

Nila hitam 3 f.gnu 956 AATTTTCCTATTATTC-TTCGGTTTTTTCGCGCG-TAAACCCCCCCT-ACCCCCCCAAAA1012 Nila putih 7 f.gnu 955 AATTTTTCTATTAACCCTTCGGTTTTTGCGCGCGGTAAACCCCCCCTTACCCCCCCAAAA1014

AATTTTCTATTA C TTCGGTTTTT CGCGCG TAAACCCCCCCT ACCCCCCCAAAA AATTTTCTATTA C TTCGGTTTTT CGCGCG TAAACCCCCCCT ACCCCCCCAAAA

Nila hitam 3 f.gnu 1013 -CTCC-AAAGATC-CTAAA-ACTCCTGCAAACCCCCC--GGAAACAGGAAAAGCTCTTA- 1065 Nila putih 7 f.gnu 1015 ACTCCTAAAAATCTCTAAATATTCCTGCAAACCCCCCCGGAAAACGGGAAAAGCTTCTAG 1074

CTCCAAA ATC CTAAA ATCCTGCAAACCCCCC G AAAC GGAAAAGCT TA

Nila hitam 3 f.gnu 1066 AATTGACTTTTAGC-GCTTTAGTTTAGGCAGGTTAAATA-ACTTAAGTGGTGGTAATGGC 1123 Nila putih 7 f.gnu 1075 AAGTAACTTTCACCCGCTTTAATAATTGCATAAAAAATTTACTTAAGTGGGGGAAATCCA 1134

AA T ACTTTA C GCTTTA T GCA AAAT ACTTAAGTGG GG AAT

Nila hitam 3 f.gnu 1124 AGATATTATCATGGCCCGGGCCATA-ATTCCAAGTGGGGGTATTTAAATTTTT--- 1175 Nila putih 7 f.gnu 1135 AGAACCTACAAAGGCACGAGTCATGTATCCCAAGTGGGTGGGTAAAAAATTAATTAAAAA1194

AGA TA A GGC CG GCAT AT CCAAGTGGG G T AAATT

Nila hitam 3 f.gnu 1175 --- 1175

Lampiran 6. Hasilalignmentsekuen DNA ikan nila putih dan ikan nila hitam dari arah reverse

Nila hitam 3 r.gnu 1 GGTTTTTTAAACCGGAATTGGGAAGGTTAAAGTCCTTCCTTATTGGCTTCAAAGGAAAAG 60 Nila putih 7 r.gnu 1 ---AATCCCTTCCCTAACCCTTTCAAAAAAAAAA31

AA CCTTCC TA TTCAAA AAAA AA CCTTCC TA TTCAAA AAAA

Nila hitam 3 r.gnu 61 GGATTTTTAACCCCTA-CCCCTTAATTCCCCAAAGCT-AGGATTCTTAACTT-AAACTAT 117 Nila putih 7 r.gnu 32 GGAATTTTAACCCTTAACCCCTTAATTCCCAAAAGGTTAGGAATTTTAATTTTAAATTAT 91

GGA TTTTAACCC TA CCCCTTAATTCCC AAAG T AGGA T TTAA TT AAA TAT

Nila hitam 3 r.gnu 118 TTTTTGGCCGAG-TTTTGCCTC-CATGCAAAATACAATACA-ATTATGTATTAT---CCC 171 Nila putih 7 r.gnu 92 TTTTTTGCCGAGGTTTTGCCTTTCATGCAAATACCAATGCCTAATATGTTTTTTTCCCCC 151

TTTTT GCCGAG TTTTGCCT CATGCAAA CAAT C A TATGT TT T CCC

Nila hitam 3 r.gnu 172 CATTATTTTATTTCAAACATATCCTATATATAAA-TACATACAACTCTTAAAAAACATAC 230 Nila putih 7 r.gnu 152 TTTTTTTTTTTTTCCAACATATCCCTAATATAAAATACATACTTTT-TTAAAAGACATCC 210

TT TTTT TTTC AACATATCC ATATAAA TACATAC T TTAAAA ACAT C

Nila hitam 3 r.gnu 231 ACTGTTTTCCC-ACATATTTGTCATCAACATCTATAACTTAGAAGAACATAAACCCATAA 289 Nila putih 7 r.gnu 211 ACTGGTTCCCCCACATATTTGCCAACAACATTTACAACTAAGAGAAACATAAACCAGTAA 270

ACTG TT CCC ACATATTTG CA CAACAT TA AACT AGA AACATAAACC TAA ACTG TT CCC ACATATTTG CA CAACAT TA AACT AGA AACATAAACC TAA

Nila hitam 3 r.gnu 290 ATGAAATATTCCAATAACGATTAAATACCACTGAACGATAGTTTAAGACCGAACACAACT 349 Nila putih 7 r.gnu 271 ATGGAACTTTCCAAAAACATTTCAAAACCACTAAACGACAGTTTAAGACCGAACACAACT 330

ATG AA TTCCAA AAC TT AA ACCACT AACGA AGTTTAAGACCGAACACAACT

Nila hitam 3 r.gnu 350 CTCATACAGTTAAGATATACCAAGTACCCAACATCCTATACTTCTAAATTATTTAATGTA 409 Nila putih 7 r.gnu 331 CTCATAC-GTTAAGATATACCAAGTACCCACCATCCTATACTTCCGAATTATTTAATGTA 389

CTCATAC GTTAAGATATACCAAGTACCCA CATCCTATACTTC AATTATTTAATGTA

Nila hitam 3 r.gnu 410 GTAAGAGCCCACCATCAGTTGATTCCTATATGTTAACGGTTCTTGAAGGTCAAGGACAAT 469 Nila putih 7 r.gnu 390 GTAAGAGCCCACCATCAGTTGATTTCTCAATGTTAACGGTTCTTGAAGGTCAAGGACAGT 449

GTAAGAGCCCACCATCAGTTGATT CT ATGTTAACGGTTCTTGAAGGTCAAGGACA T

Nila hitam 3 r.gnu 470 TATTTGTGGGGGTTTCACTAATTGAATTATTCCTGGCATCTGGTTCCTATTTCAGGTCCA 529 Nila putih 7 r.gnu 450 TATTTGTGGGGGTTTCACTAATTGAATTATTCCTGGCATCTGGTTCCTATTTCAGGTCCA 509

TATTTGTGGGGGTTTCACTAATTGAATTATTCCTGGCATCTGGTTCCTATTTCAGGTCCA

Nila hitam 3 r.gnu 530 ATAATTGTTATAATTCCCCATTCTTTTATTGACGCTTGCATAAGTTAATGGTGTCAATAC 589 Nila putih 7 r.gnu 510 ATAATTGTTATAATTCCCCATTCTTTCATTGACGCTTGCATAAGTTAATGGTGTTAATAC 569

ATAATTGTTATAATTCCCCATTCTTT ATTGACGCTTGCATAAGTTAATGGTGT AATAC

Nila hitam 3 r.gnu 590 ATACTCCTCATTACCCAACATGCCGGGCGTTCTTTCCAGAGGATAGGGGGTTTCTCTTTT 649 Nila putih 7 r.gnu 570 ATACTCCTCGTTACCCACCATGCCGGGCGTTCTTTCCAGGGTGTGGGGGGTT-CTCTTTT 628

ATACTCCTC TTACCCA CATGCCGGGCGTTCTTTCCAG G T GGGGGTT CTCTTTT

Nila hitam 3 r.gnu 650 TTTTTTTCCTTTCATTTGGCATCTCAGAGTGCATACAGAAATGACAGACAAGGTTGAACA 709 Nila putih 7 r.gnu 629 TTTTTTTCCTTTCACTTGACATTTCAGAGTGCATACAGAAAAGACAGACAAGGTTGAACA 688

TTTTTTTCCTTTCA TTG CAT TCAGAGTGCATACAGAAA GACAGACAAGGTTGAACA

Nila hitam 3 r.gnu 710 TTTTCCTTGCTTGGAGGTAAATAGTATGAATGATAGA-AGACATTGATA-GAAGTATTGC 767 Nila putih 7 r.gnu 689 TTTTCCTTGCTTGAACGGAAATAGTATGAATGGTGGTTAGATATTATTAAGAAGAATTGC 748

TTTTCCTTGCTTG A G AAATAGTATGAATG T G AGA ATT TA GAAG ATTGC

Nila hitam 3 r.gnu 768 ATAACTGATATCTAGAGCATAAAGTTCAATCAAATATTTCAATTTTCTCCTAATTTTTCT 827 Nila putih 7 r.gnu 749 ATAACTGATATCTAGAGCATAAAGTTTAATCAAA-ATTTTAATTTTCTCCTAATTTTTCT 807

ATAACTGATATCTAGAGCATAAAGTT AATCAAA ATTT AATTTTCTCCTAATTTTTCT

Nila hitam 3 r.gnu 828 ATTATTCTTCGGTTTTTTCGCGCGTAAACCCCCCCTACCCCCCCAAAACTCCTAAGATCT 887 Nila putih 7 r.gnu 808 ATTAACCTTCGGTTTTTGCGCGCGTAAACCCCCCCTACCCCCCCAAAACTCCTAAGATCT 867

ATTA CTTCGGTTTTT CGCGCGTAAACCCCCCCTACCCCCCCAAAACTCCTAAGATCT

Nila hitam 3 r.gnu 888 CTAATACTCCTGCAAACCCCCCGGAAACAGGAAAAGCTCTAGAAGTGACTTTTAGCGCTT 947 Nila putih 7 r.gnu 868 CTAATATTCCTGCAAACCCCCCGGAAACAGGAAAAGCTCTAGAAGTAACTTTCAGCGCTT 927

CTAATA TCCTGCAAACCCCCCGGAAACAGGAAAAGCTCTAGAAGT ACTTT AGCGCTT

Nila hitam 3 r.gnu 948 TAGTTTATGCATGTTATATTACTTAATGTGTGTATATGCAGTATTATCAATGCACGGGCC 1007 Nila putih 7 r.gnu 928 TAATATATGCATAAAATATTACTTAATGTGTGTATATCTAGTACTATCAATGCACGAGTC 987

TA T TATGCAT ATATTACTTAATGTGTGTATAT AGTA TATCAATGCACG G C

Nila hitam 3 r.gnu 1008 ATATATCCAATGTGTGTATATTATATTATTATAATATTGCACATGCTAGCGTAGCTTAAC 1067

Dalam dokumen Colonization and proliferation of white Nile tilapia testicular cells transplanted into black Nile tilapia triploid (Halaman 40-53)