Kesimpulan
1. Conditioned medium yang dikonsentratkan hingga 10 kali (10x-CM) dapat menunjang diferensiasi ESC mencit menjadi neural-like cells.
2. Penambahan 10x-CM ke dalam medium diferensiasi sebanyak 5% mampu mendukung perkembangan ESC mencit menjadi NLC.
3. Diferensiasi ESC mencit menjadi NLC dapat dilakukan tanpa melalui pembentukan embryoid bodies.
4. Teknik deteksi ekspresi gen dengan two steps RT-PCR diikuti analisis semikuantitatif dengan densitometer terbukti dapat dipakai untuk mendeteksi perbedaan tingkat ekspresi nestin pada tiap sampel.
Saran
1. Perlu dilakukan pengecekan terhadap faktor penginduksi yang dihasilkan pada hari ke berapa selama kultur primer syaraf.
2. Perlu adanya identifikasi kandungan protein dalam conditioned medium dengan menggunakan teknik yang lebih spesifik.
3. Dapat dilakukan penambahan periode kultur agar terlihat adanya pertumbuhan tipe neuron yang lain
4. Dapat dipertimbangkan untuk melakukan konfirmasi terhadap sel-sel yang berdiferensiasi dengan menggunakan penanda molekuler lainnya.
5. Sangat dianjurkan untuk melakukan uji imunositokimia guna mendapatkan informasi yang lebih mendukung.
6. Disarankan untuk melakukan penelitian lanjutan terhadap pemisahan protein yang berguna dalam diferensiasi.
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