Pada tahap berikutnya diperlukan pemurnian enzim kitosanase dengan teknik kromatografi yang lain sehingga dapat dilakukan perbandingan antara berbagai teknik kromatografi untuk pemurnian enzim kitosanase. Selain itu,
41 dapat pula dilakukan analisis zimogram terhadap crude enzyme dan enzim murni tersebut untuk mengetahui pita protein yang menunjukkan aktivitas kitosanase. Karakterisasi lebih lanjut terhadap enzim sebaiknya dilakukan untuk mengetahui lebih detail karakteristik kitosanase yang dihasilkan.
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