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Beberapa saran yang dapat diajukan penulis adalah sebagai berikut:

1. Untuk meningkatkan kadar pati resistan secara optimal, hidrolisis enzimatik dengan debranching enzyme sebaiknya dilakukan sebelum proses retrogradasi, sehingga pembentukan rantai linier panjang dapat optimal. 2. Penelitian secara in vitro menggunakan konsorsium bakteri fecal perlu

dilakukan untuk mengetahui kemampuan prebiotik didegradasi oleh konsorsium bakteri serta profil asam lemak rantai pendek yang dihasilkan. 3. Fermentasi in vitro menggunakan RS beras Cisokan dan RS sagu perlu

dilakukan lebih dari 48 jam, sehingga pola pertumbuhan bakteri mencapai fase kematian dan kadar butirat yang dihasilkan lebih tinggi.

4. Untuk meningkatkan kadar butirat dapat dilakukan cara : 1) meningkatkan kadar RS pada medium, 2) Memperpanjang waktu fermentasi dan 3) meningkatkan aktivitas enzim Co-A transferase dan butirat kinase, salah satunya dengan modifikasi pH medium dan penambahan sodium asetat.

DAFTAR PUSTAKA

Amans, S.S., Girbal,L., Andrade, J., Ahrens, K., and Soucaille, P. 2001. Regulation of carbon and electron flow in Clostridium butyricum VPI 3266 grown on glucose-glycerol mixtures. J. Bacteriol. vol. 183 no 5 :1748–1754 Amelsvoort, V., ASP, N.G., and Hautvast, J.G.A.J. 1996. Nutritional Implications

of Resistant Starch. Nutrition Research Reviews.

Andoh, A., Tsujikawa, T., and Fujiyama, Y. 2003. Role of dietary fiber and short- chain fatty acids in the colon. In Nugent, A.P. 2005. Review Health Properties of Resistant Starch. J. Br Nutr. 30: 27-54.

AOAC. 1990. Method 988.12. Dextran in Raw Cane Sugar

AOAC. 1995. Official Methodes of Analysis of Association of Official Analytical Chemistry, Washington, DC.

Apriyantono, A., Fardiaz, D., Puspitasari, N.L., Yasni, S., and Budiyanto, S. 1989. Petunjuk Praktikum Analisis Pangan. IPB Press, Bogor.

Ballongue, J., Amine, J., Petitdemange, H., and Gay, R. 1986. Regulation of acetate kinase and butirate kinase by acids in Clostridium acetobutylicum. Lett. FEMS Microbiol. vol 35, 2-3: 295-301.

Brouns, F., Kettlitz, B., and Arrigoni, E. 2002. Resistant starch and butyrate revolution. J. Trends Food Sci. Technol., 13 : 251-261.

Casciato, D.A., Stewart, P.R., and Rosenblatt, J.E. 1974. Growth curves of anaerobic bacteria in solid media. J. Appl. Microbiol. May 1975: 610-614. Casterline, J.L., Oles, C.J., and Ku, Y. 1997. In vitro fermentation of varios food

fiber fractions. J. Agric. Food Chem. 45 (7).

Chen, C.K and Blaschek, H.P. 1999. Effect of acetate on molecular and physiological aspects of Clostridium beijerinckii NCIMB 8052 solvent production and strain degeneration. J. Appl. Environ. Microbiol. vol 65 no 2: 499-505.

Childs, N.W. 2004. Production and utilization of rice. In Elaine t. Champagne (editor). Rice : Chemistry and Technology. American Association of Cereal Chemist, Inc, Minnesota.

Croghan, M. 1992. Resistant Starch as a Functional Ingridient in Food Systems. A reference Section : Nutrition at National starch.

Cummings, J.H. 1997. The large intestine in nutrition and disease. Brussels: Institut Danone.

Drochner, W., Kerler, A., and Zacharias, B. 2004. Pectin in pig nutrition, a comparative review. J. Anim. Physiol. Anim. Nutr. 88: 367-380.

Duncan, S.H., Louis, P. and Flint, H.J. 2004. Lactate-utilising bacteria, isolated from human feces, that produce butyrate as a major fermentation product. J. Appl. Microbiol. 70: 5810-5817.

Escarpa, M. C., Gonzlez, E., Maas, L., Garca-Diz, and Calixto, F.S. 1996 Resistant Starch Formation: Standardization of a High-Pressure Autoclave Process. J. Agric. Food Chem. 44 (3): 924-928

Fen, H.L. 2007. Physicochemical and functional properties of enzyme modified tapioca starches [Thesis]. Malaysia: Faculty of Sains, Universiti Sains Malaysia.

Frobisher, M. 1962. Microbiology 7th edition. W.B Saunders Company, Philadelphia.

Goni, I., Gracia-Diz, L., Manas, E., and Calixto, F.S. 1996. Analysis of resistant starch: a method for foods and food products. J. Food Chem. vol 56 no 4: 445-449.

Goni, I., and Alonso, A.G. 2000. Effect of processing on potato starch: in vitro avaibility and glicaemic index. J.Starch 52 Nr. 2-3: 81-84.

Harrigan, W.F. 1998. Laboratory Methods in Food Microorganisms 3rd edition. Academic Press, San Diego.

Jensen, M.T., Cox, R.P. and Jensen, B.B. 1995. Microbial production of skatole in the hind gut of pigs given different diets and its relation to skatole deposition in backfat. J. Anim. Sci. 61: 293-304.

Kettlitz, B.W., Coppin, Marie, J.V.J.R., Bornet, H.W.W., and Francis. 1997. Highly fermentable resistant starch. In pharmaceutical Patent 6,043,229. December 2, 1997.

Khan, K.M., and Edwards, C.A. 2005. In vitro fermentation characteristics of a mixture of raftilose and guar gum by human faecal bacteria. Eur J Nutr. 44 : 371–376.

Lehmann, U., Jacobasch, G., and Schmiedl, D. 2002. characterization of resistant starch type III from banana (Musa acuminata). J. Agric. Food Chem. 50: 5236-5240

Louis, P., Duncan, S.H., McCrae, S.I.,Millar, J., Jackson, M.S., and Flint, H.J. 2004. Restricted distribution of the butyrate kinase pathway among butyrate-producing bacteria from the human colon. J. Bacteriol. vol 186 no 7: 2099-2106.

Louis, P., Scott, K.P., Duncan, S.H., and Flint, H.J. 2007. Review article: Understanding the effects of diet on bacterial metabolism in the large intestine. J. Appl. Microbiol. 102: 1197-1208.

Mangala, R., Silanere, L., and Tharanathan. 1999. Structural studies of resistant starch derived from processed (autoclaved) rice. J. Eur Food Res Technol 209 : 38–42.

McBurney, M. I.,and Thompson, L. U. 1987. Effect of human faecal inoculum on in vitro fermentation variables. Br. J. Nutr 58: 233-243.

Miwatani, T. 1990. A Profile of Intestinal Bacteria. Tomotari Mitsuoka (ed). Yakult Honsha Co, Ltd, Tokyo.

Montagne, L., Pluske J.R. and Hampson, D.J. 2003. A review of interactions between dietary fibre and the intestinal mucosa, and their consequences on digestive health in young non-ruminant animals. J. Anim. Feed Sci. Technol. 108: 95-117.

Nugent, A.P. 2005. Review: health properties of resistant starch. British Nutrition Foundation Nutrition Bulletin, 30: 27-54.

Ozturk, S., Koksel, H., Kahraman, K., and Perry K. W. Ng. 2009. Effect of debranching and heat treatments on formation and functional properties of resistant starch from high-amylose corn starches. J. Eur Food Res Technol. February 2009

Pelczar, M.J and Chan, E.C.S. 2005. Dasar-Dasar Mikrobiologi. Hadioetomo, R.S., Imas, T., Tjitrosomo, S.S., dan angka, S.L, penerjemah. UI press: Jakarta. Terjemahan dari: Elements of Microbiology.

Pongjanta, J., Utaipattanaceep, A., Naivikul, O., and Piyachomkwan, K. 2009. Debranching enzyme concentration effected on physicochemical properties and α-amylase hydrolysis rate of resistant starch type III from amylose rice starch. J. Carbohydr Polymer. 78: 5–9.

Roberfroid, M. B. 2001. Prebiotics : Preferential substrates for spesific germs?. Am J. Clin Nutr 2001;73 (suppl) : 406S–9S.

Sajilata, M.G, and Singhal, R.S. 2005. Specialty starches for snack foods. J. Carbohydr. Polymer. 59:131–51.

Sajilata, M.G., Singhai, R.S., and Kulkarni, P.R. 2006. Resistant Starch- A review. In Comprehensive Reviewa in Food Science and Food Safety vol 5. Sanz, M.L., Cote, G.L., Gibson, G. R., and Rastall, R.A. 2005. Prebiotic properties of alternansucrase maltose-acceptor oligosaccharides. J. Agric. Food Chem. 53: 5911-5916.

Sarles, W. B., Frazier, W.C., Wilson, J.B., and Knight, S.G. 1956. Microbiology General and Applied 2nd ed. Harper and Brothers, New York.

Sasaki, T., Kohyama, K., Suzuki, Y., Okamoto, K., Noel, T.R., and Ring, S.G. 2009. Physicochemical characteristics of waxy rice starch influencing the in vitro digestibility of starch gel. J. Food Chem. 116: 137-142.

Sayar, S., Jannink, J., and White, P.J. 2007. Digestion residues of typical and high-â-Glucan oat flours provide substrates for in vitro fermentation. J. Agric. Food Chem. 55: 5306-5311.

Schwiertz, A., Lehmann, U., Jacobasch, G., and Blaut, M. 2002. Influence of resistant starch on the SCFA production and cell counts of butyrate- producing Eubacterium spp. in the human intestine. J. Appl. Microbiol. 93 (1): 157–162.

SNI 01-2892-1992. Metode analisis total pati. Standar Nasional Indonesia.

Theodoratou, E,. McNeill G., Cetnarsky R., Farrington S.M., Tenesa A., Barnetson R., Porteous M., Dunlop M., and Campbell M. 2007. Dietary

fatty acids and colorectal cancer: A case-control study. J. Epidemiol. Vol 166 no 2.

Todar, K. 2007. Growth of Bacterial Populations. Department of Bacteriology, University of Wisconsin-Madison.

Tovar, F., Bjarck, I. M. and Asp, N.G. 1992. Incomplete digestion of legume starches in rats: a study of precooked flours containing retrograded and physically inaccessible starch fractions. J. Nutr. 122: 1500-1507.

Wang, X., Conway, P.L., Brown, I.L., and Evans, A.J. 1999. In vitro utilization of amylopectin and high-amylose maize (Amylomaize) starch granules by human colonic bacteria. J. Appl. Environ. Microbiol. 65: 4848-4854.

Wasserman, L.A., Signorelli, M., Schiraldi, A., Yuryev, V., Boggini, G., Bertini,S., and Fessas, S. 2007. Preparation of wheat resistant starch: treatment of gels and DSC characterization. J. Therm. Anal. Cal. Vol. 87. 1: 153–157.

Whelan, W. J. and Nasr, H. 1950. The Amylase of Clostridium butyricum. Department of Chemistry, University College of North Wales, Bangor and Rowett Research Institute, Bucksburn, Aberdeenshire, vol 48.

Wiesenborn, D.P., Rudolph, F.B., and Papoutsakins, E.T. 1989. Coenzyme a transferase from Clostridium acetobutylicum ATCC 824 and its role in the uptake of acids. J. Appl. Envir. Microbiol. vol 55 no.2: 323-329.

Winarno, F.G. 1997. Kimia Pangan dan Gizi. Gramedia, Jakarta.

Wong, K.S., McPherson, A.E., and Jane, J.L. 1997. Branch-structure difference in starches of A and B-type X-ray patterns revealed by their Naegeli dextrins. J. Carbohydr. Res. 300: 219-227.

Zhao, X.H. and Lin, Y. 2009. Resistant starch prepared from high-amylose maize starch with citric acid hydrolysis and its simulated fermentation in vitro. J. Eur Food Res Technol 228: 1015-1021.

Zhu, Y and Yang, S. 2004. Effect of pH on metabolic pathway shift in

fermentation of xylose by Clostridium tyrobutyricum. J. Biotechnol. vol 110 (2): 143-157.

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