BAB VII SIMPULAN DAN SARAN
6.2 Saran
1. Perlu dilakukan penelitian lebih lanjut terhadap variasi konsentrasi ekstrak etanol daun pandan wangi yang mampu menurunkan immobility time dan kadar kortisol tikus jantan galur wistar yang depresi.
2. Perlu dilakukan penelitian lebih lanjut dengan melakukan isolasi komponen aktif dari ekstrak pandan wangi dengan pelarut yang spesifik, serta penetapan kadar secara kuantitatif untuk mengetahui komponen kimia yang memiliki efek paling besar dalam menurunkan immobility time dan kadar kortisol tikus jantan galur wistar yang depresi.
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(Pandanus amaryllifolius R.)
a. Hasil Analisis Deskriptif
Kelompok Statistic Std. Error
5% Trimmed Mean 88.1296
Median 88.0000
Kontrol Negatif Mean 1.3067E2 .61464
95% Confidence Interval for Mean
Lower Bound 1.2909E2 Upper Bound 1.3225E2
5% Trimmed Mean 1.3074E2
Median 1.3100E2
Kontrol Positif Mean 1.3167E2 .66667
95% Confidence Interval for Mean
Lower Bound 1.2995E2 Upper Bound 1.3338E2
5% Trimmed Mean 1.3163E2
Median 1.3150E2
Variance 2.667
Minimum 130.00
5% Trimmed Mean 1.3031E2
Median 1.3050E2
Kontrol Negatif Mean 128.167 .6009
95% Confidence Interval for Mean
Lower Bound 126.622 Upper Bound 129.711
5% Trimmed Mean 128.185
Median
Std. Deviation 1.4720
Kontrol Positif Mean 48.667 .3333
95% Confidence Interval for
Kelompok
Shapiro-Wilk Statistic df Sig.
Pre-Immobility Time Kelompok Normal .908 6 .421
Kontrol Negatif .866 6 .212
Kontrol Positif .920 6 .505
Ekstrak 10% .974 6 .918
Post-Immobility Time Kelompok Normal .915 6 .473
Kontrol Negatif .958 6 .804
Kontrol Positif .822 6 .091
Ekstrak 10% .907 6 .415
c. Uji Homogenitas Levene Test
Levene Statistic df1 df2 Sig.
Dependent
Pair 1 Pre-Immobility Time (K1) &
Post-Immobility Time (K1) 6 -.607 .201
Paired Differences
Deviation Std. Error Mean Pair 1 Pre-Immobility Time 1.3067E2 6 1.50555 .61464
Post (K2)-Immobility
Time (K2) 128.167 6 1.4720 .6009
Paired Samples Correlations
N Correlation Sig.
Pair 1 Pre-Immobility Time (K2) &
Post-Immobility Time (K2) 6 -.331 .522
Paired Samples Statistics
Pair 1 Pre-Immobility Time (K3) &
Post-Immobility Time (K3) 6 .500 .312
Post-Immobility Time 71.333 6 1.2111 .4944
Paired Samples Correlations
N Correlation Sig.
Pair 1 Pre-Immobility Time &
Post-Immobility Time 6 -.157 .766
Paired Differences
t df Sig.
(2-tailed) Mean
Std.
Deviation
Std. Error Mean
95% Confidence Interval of the
Difference Lower Upper Pair 1
Pre-Immobility Time - Post-Immobility Time
5.90000E1 2.28035 .93095 56.60692 61.39308 63.376 5 .000
a. Hasil Analisis Deskriptif
Descriptives
Kelompok Statistic Std. Error
5% Trimmed Mean 15.3493
Median 15.2700
5% Trimmed Mean 20.4584
Median 20.5805
5% Trimmed Mean 20.4823
Median 20.2995
Variance .303
Maximum 21.18
5% Trimmed Mean 21.0067
Median 21.0070
Minimum 20.2
Kelompok
Shapiro-Wilk
Statistic df Sig.
Pre-Kortisol Kelompok Normal .859 6 .186
Kontrol Negatif .906 6 .411
Kontrol Positif .808 6 .069
Ekstrak 10% .981 6 .958
Post-Kortisol Kelompok Normal .978 6 .942
Kontrol Negatif .863 6 .200
Kontrol Positif .806 6 .066
Ekstrak 10% .867 6 .213
c. Uji Homogenitas Levene Test
Levene Statistic df1 df2 Sig.
Pre-Kortisol .475 3 20 .703
Post-Kortisol 2.242 3 20 .115
d. Uji ANOVA
ANOVA
Sum of Squares df Mean Square F Sig.
Pre-Kortisol Between Groups 126.536 3 42.179 173.558 .000
Within Groups 4.860 20 .243
Total 131.396 23
Post-Kortisol Between Groups 225.099 3 75.033 221.167 .000
Within Groups 6.785 20 .339
Total 231.884 23
Dependent
N Correlation Sig.
Pair 1 Pre-Kortisol (K1) &
Post-Kortisol (K1) 6 .845 .034
Paired Samples Statistics
Pair 1 Pre-Kortisol (K3) &
Post-Kortisol (K3) 6 -.240 .647
Pair 1 Pre-Kortisol (K4)& Post-Kortisol
(K4) 6 .399 .434
Tanaman Pandan wangi yang digunakan
Pemilihan daun pandan wangi
Ekstraksi Penguapan ekstrak dengan rotary evaporator
Metode tail suspention test Metode Forced Swimming Test
Pemberian obat dan ekstrak
Keterangan:
a. Anestesi hewan uji dengan ketamine secara intraperitoneal b. Pengambilan darah hewan uji melalui jantung
c. Pemindahan darah kedalam tabung efendrof d Darah hewan uji dalam tabung efendrof e. Mesin sentrifugasi
f. hasil sentrifugasi
a b
c
d e
f
e
Keterangan:
a. Reagen Kit ELISA-cortisol b. Proses pengerjaan ELISA
c. Proses pembacaan dengan ELISA reader
a b
c