51
Lampiran 1. Pengolahan Tepung KBMfermentasi Phanerochaete chrysosporium
Kulit Buah Markisa (KBM) SEGAR
Dicuci
Jemur dibawah sinar matahari (3 hari)
Dicacah
Digiling
Tepung KBM
Disterilkan/diautoclave
(15 menit, 1210C)
Tepung KBM +Phanerochaete chrysosporium
Fermentasi selama 15 hari (suhu kamar, Dosis 106 CFU/ml, 1,18 ml suspensi spora untuk
1 gram Tepung KBM)
Pengeringan
Dioven
(24 jam, 600C)
KBM PRODUK FERMENTASI
52
Lampiran 2. Pembuatan Pakan Bentuk Pelet
Bahan Baku
Bahan Baku digiling menjadi Tepung
Ditimbang menurut formulasi
Diaduk rata di tempat pengadukan
Penambahan bahan baku cair (kalau dibutuhkan)
Diaduk lagi sampai bahan baku cair tersebut
dapat tercampur merata ke seluruh bagian
Bahan baku berbentuk adonan(kebasahan 60%)
Dimasukkan ke alat pencetak pelet
Dihasilkan ukuran pelet 5-7 mm
Pelet dikeringkan (dioven selama 12 jam,
temperatur 500C)
53
Lampiran 3. Hasil Analisis Proksimat Kulit Buah Markisa yang difermentasi dengan Phanerochaete chrisosporium selama 15 hari.
Pelet siap diberikan
54
Lampiran 4. Data Bobot Awal (g), Bobot Akhir (g), Bobot Potong (g), Bobot Karkas (g) dan Persentase Karkas (%)
55
Perlakuan BB Awal BB Akhir Bobot
Potong
Bobot
Karkas % Karkas
P0U1 886 1818 1800 924 51,33
P0U2 896 1692 1674 876 52,33
P0U3 992 1780 1762 910 51,65
P0U4 983 1803 1786 918 51,40
P0U5 934 1786 1776 914 51,46
Total 4691 8879 8798 4542 258,17
Rata-rata 938,2 1775,8 1759,6 908,4 51,63
P1U1 942 1869 1858 965 51,94
P1U2 1006 1735 1724 903 52,38
P1U3 960 1865 1855 968 52,18
P1U4 808 1862 1848 942 50,97
P1U5 800 1945 1930 994 51,50
Total 4516 9276 9215 4772 258,97
Rata-rata 903,2 1855,2 1843 954,4 51,79
P2U1 845 1878 1856 966 52,05
P2U2 828 1888 1875 977 52,11
P2U3 821 1919 1900 988 52,00
P2U4 1046 1886 1872 966 51,60
P2U5 1032 1771 1761 905 51,39
Total 4572 9342 9264 4802 259,15
Rata-rata 914,4 1868,4 1852,8 960,4 51,83
P3U1 880 1969 1958 1018 51,99
P3U2 978 1937 1925 1013 52,62
P3U3 915 1797 1780 937 52,64
P3U4 936 1872 1861 982 52,77
P3U5 871 1865 1853 965 52,08
Total 4580 9440 9377 4915 262,10
Rata-rata 916 1888 1875,4 983 52,42
Lampiran 5. Data Penimbangan Komponen Non Karkas (g/ekor)
Perlakuan Bobot Organ Dalam (g) Bobot Organ Luar (g)
56
Saluran
Pencernaan Jantung Hati
Paru-Paru Ginjal Kepala Kaki Kulit
P0U1 185,00 6,00 50,00 15,00 15,00 113,00 53,00 268,00
P0U2 162,00 5,00 45,00 10,00 10,00 101,00 50,00 250,00
P0U3 178,00 5,00 47,00 13,00 11,00 110,00 51,00 260,00
P0U4 179,00 5,00 48,00 14,00 13,00 111,00 52,00 266,00
P0U5 179,00 6,00 49,00 13,00 12,00 110,00 51,00 263,00
Total 883,00 27,00 239,00 65,00 61,00 545,00 257,00 1307,00
Rata-rata 176,60 5,40 47,80 13,00 12,20 109,00 51,40 261,40
P1U1 184,00 6,00 50,00 14,00 14,00 114,00 54,00 274,00
P1U2 167,00 6,00 49,00 11,00 11,00 112,00 52,00 262,00
P1U3 180,00 6,00 51,00 14,00 14,00 114,00 54,00 274,00
P1U4 179,00 6,00 50,00 14,00 13,00 113,00 54,00 272,00
P1U5 183,00 5,00 51,00 16,00 16,00 118,00 56,00 298,00
Total 893,00 29,00 251,00 69,00 68,00 571,00 270,00 1380,00
Rata-rata 178,60 5,80 50,20 13,80 13,60 114,20 54,00 276,00
P2U1 175,00 7,00 53,00 15,00 14,00 114,00 54,00 278,00
P2U2 176,00 6,00 51,00 14,00 14,00 116,00 55,00 280,00
P2U3 195,00 5,00 50,00 16,00 16,00 118,00 56,00 298,00
P2U4 184,00 5,00 53,00 13,00 14,00 115,00 55,00 281,00
P2U5 164,00 7,00 51,00 12,00 11,00 112,00 53,00 270,00
Total 894,00 30,00 258,00 70,00 69,00 575,00 273,00 1407,00
Rata-rata 178,80 6,00 51,60 14,00 13,80 115,00 54,60 281,40
P3U1 188,00 7,00 56,00 16,00 15,00 120,00 58,00 303,00
P3U2 185,00 7,00 55,00 15,00 15,00 119,00 57,00 294,00
P3U3 168,00 6,00 48,00 13,00 11,00 113,00 53,00 268,00
P3U4 179,00 7,00 53,00 14,00 15,00 116,00 55,00 287,00
P3U5 177,00 6,00 52,00 15,00 14,00 115,00 55,00 286,00
Total 897,00 33,00 264,00 73,00 70,00 583,00 278,00 1438,00
Rata-rata 179,40 6,60 52,80 14,60 14,00 116,60 55,60 287,60
Lampiran 6. Data Bobot Relatif Komponen Non Karkas (g/kg BB)
57
Perlakuan
Bobot Relatif Organ Dalam Bobot Relatif Organ Luar
Slrn
Pencernaan Jantung Hati
Paru-Paru Ginjal Kepala Kaki Kulit
P0U1 102,78 3,33 27,78 8,33 8,33 62,78 29,44 148,89
P0U2 96,77 2,99 26,88 5,97 5,97 60,33 29,87 149,34
P0U3 101,02 2,84 26,67 7,38 6,24 62,43 28,94 147,56
P0U4 100,22 2,80 26,88 7,84 7,28 62,15 29,12 148,94
P0U5 100,79 3,38 27,59 7,32 6,76 61,94 28,72 148,09
Total 501,59 15,34 135,8 36,84 34,59 309,63 146,09 742,81
Rata-rata 100,32 3,07 27,16 7,37 6,92 61,93 29,22 148,56
P1U1 99,03 3,23 26,91 7,53 7,53 61,36 29,06 147,47
P1U2 96,87 3,48 28,42 6,38 6,38 64,97 30,16 151,97
P1U3 97,04 3,23 27,49 7,55 7,55 61,46 29,11 147,71
P1U4 96,86 3,25 27,06 7,58 7,03 61,15 29,22 147,19
P1U5 94,82 2,59 26,42 8,29 8,29 61,14 29,02 154,4
Total 484,61 15,78 136,31 37,33 36,79 310,06 146,57 748,74
Rata-rata 96,92 3,16 27,26 7,47 7,36 62,01 29,31 149,75
P2U1 94,29 3,77 28,56 8,08 7,54 61,42 29,09 149,78
P2U2 93,87 3,20 27,20 7,47 7,47 61,87 29,33 149,33
P2U3 102,63 2,63 26,32 8,42 8,42 62,11 29,47 156,84
P2U4 98,29 2,67 28,31 6,94 7,48 61,43 29,38 150,11
P2U5 93,13 3,98 28,96 6,81 6,25 63,60 30,10 153,32
Total 482,21 16,25 139,34 37,73 37,16 310,43 147,38 759,39
Rata-rata 96,44 3,25 27,87 7,55 7,43 62,09 29,48 151,88
P3U1 96,02 3,58 28,60 8,17 7,66 61,29 29,62 154,75
P3U2 96,10 3,64 28,57 7,79 7,79 61,82 29,61 152,73
P3U3 94,38 3,37 26,97 7,30 6,18 63,48 29,78 150,56
P3U4 96,18 3,76 28,48 7,52 8,06 62,33 29,55 154,22
P3U5 95,52 3,24 28,06 8,09 7,56 62,06 29,68 154,34
Total 478,21 17,58 140,68 38,89 37,25 310,98 148,24 766,60
Rata-rata 95,64 3,52 28,14 7,78 7,45 62,20 29,65 153,32
Lampiran 7. Analisis Keragaman Bobot Potong Kelinci Rex
58
Lampiran 12. Analisis Keragaman Bobot Relatif Hati Kelinci Rex
59
SK dB JK KT F-Hit F-Tabel
0,05 0,01
Perlakuan 3 0,46 0,15 0,324tn 3,24 5,29
Galat 16 7,48 0,47
Total 19 7,94
Ket : tntidak berbeda nyata
Lampiran 14. Analisis Keragaman Bobot Relatif Ginjal Kelinci Rex
SK dB JK KT F-Hit F-Tabel
0,05 0,01
Perlakuan 3 0,95 0,32 0,501tn 3,24 5,29
Galat 16 10,06 0,63
Total 19 11,01
Ket : tntidak berbeda nyata
Lampiran 15. Analisis Keragaman Bobot Relatif Kepala Kelinci Rex
SK dB JK KT F-Hit F-Tabel
0,05 0,01
Perlakuan 3 0,20 0,07 0,052tn 3,24 5,29
Galat 16 20,40 1,28
Total 19 20,60
Ket : tntidak berbeda nyata
Lampiran 16. Analisis Keragaman Bobot Relatif Kaki Kelinci Rex
SK dB JK KT F-Hit F-Tabel
0,05 0,01
Perlakuan 3 0,54 0,18 1,232tn 3,24 5,29
Galat 16 2,23 0,15
Total 19 2,85
Ket : tntidak berbeda nyata
Lampiran 17. Analisis Keragaman Bobot Relatif Kulit Kelinci Rex
SK dB JK KT F Hit F-Tabel
0,05 0,01
Perlakuan 3 68,01 22,67 3,731* 3,24 5,29
Galat 16 97,21 6,08
Total 19 165,21
60
60
Lampiran 18. Analisis Keragaman Pengaruh Pemberian Tepung Kulit Buah Markisa Fermentasi Phanerochaete chrysosporium terhadap Bobot Potong
The SAS System 12:37 Thursday, October 28, 2015 2
The GLM Procedure
Dependent Variable: Bobot Potong
Sum of
Source DF Squares Mean Square F Value Pr > F Model 3 38385.0000 12795.0000 3.27 0.0488 Error 16 62675.2000 3917.2000
Corrected Total 19 101060.2000
R-Square Coeff Var Root MSE Bobot Potong Mean 0.379823 3.415045 62.58754 1832.700
Source DF Type I SS Mean Square F Value Pr > F PERLAKUAN 3 38385.00000 12795.00000 3.27 0.0488
Source DF Type III SS Mean Square F Value Pr > F PERLAKUAN 3 38385.00000 12795.00000 3.27 0.0488
The GLM Procedure
Duncan's Multiple Range Test for Bobot Potong
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 3917.2
Number of Means 2 3 4 Critical Range 83.91 88.00 90.55
Means with the same letter are not significantly different.
Duncan Grouping Mean N PERLAKUAN
A 1875.40 5 P3 A
A 1852.80 5 P2 A
B A 1843.00 5 P1 B
B 1759.60 5 P0
61
Lampiran 19. Analisis Keragaman Pengaruh Pemberian Tepung Kulit Buah Markisa Fermentasi Phanerochaete chrysosporium terhadap Bobot Karkas.
The SAS System 12:37 Thursday, October 28, 2015 6
The GLM Procedure
Dependent Variable: Bobot Karkas
Sum of
Source DF Squares Mean Square F Value Pr > F Model 3 14687.35000 4895.78333 5.29 0.0100 Error 16 14815.60000 925.97500
Corrected Total 19 29502.95000
R-Square Coeff Var Root MSE Bobot Karkas Mean 0.497826 3.197923 30.42984 951.5500
Source DF Type I SS Mean Square F Value Pr > F PERLAKUAN 3 14687.35000 4895.78333 5.29 0.0100
Source DF Type III SS Mean Square F Value Pr > F PERLAKUAN 3 14687.35000 4895.78333 5.29 0.0100
The GLM Procedure
Duncan's Multiple Range Test for Bobot Karkas
NOTE : This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05
Error Degrees of Freedom 16 Error Mean Square 925.975
Number of Means 2 3 4 Critical Range 40.80 42.78 44.02
Means with the same letter are not significantly different.
Duncan Grouping Mean N PERLAKUAN
A 983.00 5 P3 A
A 960.40 5 P2 A
A 954.40 5 P1
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Lampiran 20. Analisis Keragaman Pengaruh Pemberian Tepung Kulit Buah Markisa Fermentasi Phanerochaete chrysosporium terhadapPersentaseBobot Karkas
The SAS System 12:37 Thursday, October 28, 2015 10
The GLM Procedure
Dependent Variable: Persentase Karkas
Sum of
Source DF Squares Mean Square F Value Pr > F Model 3 1.76886000 0.58962000 3.29 0.0477 Error 16 2.86352000 0.17897000
Corrected Total 19 4.63238000
R-Square Coeff Var Root MSE Persentase Karkas Mean 0.381847 0.814824 0.423048 51.91900
Source DF Type I SS Mean Square F Value Pr > F PERLAKUAN 3 1.76886000 0.58962000 3.29 0.0477
Source DF Type III SS Mean Square F Value Pr > F PERLAKUAN 3 1.76886000 0.58962000 3.29 0.0477
The GLM Procedure
Duncan's Multiple Range Test for Persentase Karkas
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05
Error Degrees of Freedo 16 Error Mean Square 0.17897
Number of Means 2 3 4 Critical Range .5672 .5948 .6120
Means with the same letter are not significantly different.
Duncan Grouping Mean N PERLAKUAN
A 52.4180 5 P3
B 51.8300 5 P2 B
B 51.7940 5 P1 B
B 51.6340 5 P0
63
Lampiran 21. Analisis Keragaman Pengaruh Pemberian Tepung Kulit Buah Markisa Fermentasi Phanerochaete chrysosporium terhadap Bobot Relatif Jantung
The SAS System 12:37 Thursday, October 28, 2015 14
The GLM Procedure
Dependent Variable: Bobot Relatif Jantung
Sum of
Source DF Squares Mean Square F Value Pr > F Model 3 0.53484000 0.17828000 1.35 0.2934 Error 16 2.11208000 0.13200500
Corrected Total 19 2.64692000
R-Square Coeff Var Root MSE Bobot Relatif Jantung Mean 0.202061 11.01652 0.363325 3.298000
Source DF Type I SS Mean Square F Value Pr > F PERLAKUAN 3 0.53484000 0.17828000 1.35 0.2934
Source DF Type III SS Mean Square F Value Pr > F PERLAKUAN 3 0.53484000 0.17828000 1.35 0.2934
The SAS System 12:37 Thursday, October 28, 2015 15
The GLM Procedure
Duncan's Multiple Range Test for Bobot Relatif Jantung
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05
Error Degrees of Freedom 16 Error Mean Square 0.132005
Number of Means 2 3 4 Critical Range .4871 .5108 .5256
Means with the same letter are not significantly different.
Duncan Grouping Mean N PERLAKUAN
A 3.5180 5 P3 A
A 3.3560 5 P1 A
A 3.2500 5 P2 A
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Lampiran 22. Analisis Keragaman Pengaruh Pemberian Tepung Kulit Buah Markisa Fermentasi Phanerochaete chrysosporium terhadapBobot Relatif Paru-paru
65
Lampiran 23. Analisis Keragaman Pengaruh Pemberian Tepung Kulit Buah Markisa Fermentasi Phanerochaete chrysosporium terhadap Bobot Relatif Ginjal
The SAS System 12:37 Thursday, October 28, 2015 22
The GLM Procedure
Dependent Variable: Bobot Relatif Ginjal
Sum of
Source DF Squares Mean Square F Value Pr > F Model 3 0.94993500 0.31664500 0.50 0.6850 Error 16 10.05272000 0.62829500
Corrected Total 19 11.00265500
R-Square Coeff Var Root MSE Bobot Relatif Ginjal Mean 0.086337 10.87536 0.792651 7.288500
Source DF Type I SS Mean Square F Value Pr > F PERLAKUAN 3 0.94993500 0.31664500 0.50 0.6850
Source DF Type III SS Mean Square F Value Pr > F PERLAKUAN 3 0.94993500 0.31664500 0.50 0.6850
The SAS System 12:37 Thursday, October 28, 2015 23
The GLM Procedure
Duncan's Multiple Range Test for Bobot Relatif Ginjal
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05
Error Degrees of Freedom 16 Error Mean Square 0.628295
Number of Means 2 3 4 Critical Range 1.063 1.114 1.147
Means with the same letter are not significantly different.
Duncan Grouping Mean N PERLAKUAN
A 7.4500 5 P3 A
A 7.4320 5 P2 A
A 7.3560 5 P1 A
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Lampiran 25. Analisis Keragaman Pengaruh Pemberian Tepung Kulit Buah Markisa Fermentasi Phanerochaete chrysosporium terhadap Bobot Relatif Saluran Pencernaan
The SAS System 12:37 Thursday, October 28, 2015 30
The GLM Procedure
Dependent Variable: Bobot Relatif Saluran Pencernaan
Sum of
Source DF Squares Mean Square F Value Pr > F Model 3 63.6121750 21.2040583 3.59 0.0371 Error 16 94.4268800 5.9016800
Corrected Total 19 158.0390550
R-Square Coeff Var Root MSE Bobot Relatif Saluran Pencernaan Mean 0.402509 2.495942 2.429337 97.33150
Source DF Type I SS Mean Square F Value Pr > F PERLAKUAN 3 63.61217500 21.20405833 3.59 0.0371
Source DF Type III SS Mean Square F Value Pr > F PERLAKUAN 3 63.61217500 21.20405833 3.59 0.0371
The SAS System 12:37 Thursday, October 28, 2015 31
The GLM Procedure
Duncan's Multiple Range Test for Bobot Relatif Saluran Pencernaan
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05
Error Degrees of Freedom 16 Error Mean Square 5.90168
Number of Means 2 3 4 Critical Range 3.257 3.416 3.515
Means with the same letter are not significantly different.
Duncan Grouping Mean N PERLAKUAN
A 100.316 5 P0
B 96.928 5 P1 B
B 96.442 5 P2 B
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Lampiran 26. Analisis Keragaman Pengaruh Pemberian Tepung Kulit Buah Markisa Fermentasi Phanerochaete chrysosporium terhadapBobot Relatif Kepala
69
Lampiran 27. Analisis Keragaman Pengaruh Pemberian Tepung Kulit Buah Markisa Fermentasi Phanerochaete chrysosporium terhadap Bobot Relatif Kaki
The SAS System 14:35 Thursday, October 28, 2015 6
The GLM Procedure
Dependent Variable: BobotRelatifKaki
Sum of
Source DF Squares Mean Square F Value Pr > F Model 3 0.95353500 0.31784500 4.54 0.0174 Error 16 1.11896000 0.06993500
Corrected Total 19 2.07249500
R-Square Coeff Var Root MSE BobotRelatifKaki Mean 0.460090 0.900892 0.264452 29.35450
Source DF Type I SS Mean Square F Value Pr > F PERLAKUAN 3 0.95353500 0.31784500 4.54 0.0174
Source DF Type III SS Mean Square F Value Pr > F PERLAKUAN 3 0.95353500 0.31784500 4.54 0.0174
The SAS System 14:35 Thursday, October 28, 2015 7
The GLM Procedure
Duncan's Multiple Range Test for BobotRelatifKaki
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05
Error Degrees of Freedom 16 Error Mean Square 0.069935
Number of Means 2 3 4 Critical Range .3546 .3718 .3826
Means with the same letter are not significantly different.
Duncan Grouping Mean N PERLAKUAN
A 29.6480 5 P3 A
B A 29.4740 5 P2 B
B C 29.1980 5 P1 C
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Lampiran 28. Analisis Keragaman Pengaruh Pemberian Tepung Kulit Buah Markisa Fermentasi Phanerochaete chrysosporium terhadap Bobot Relatif Kulit