Fernando 22010112130120 Lap.KTI Bab7

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Lampiran

Lampiran 1. Hasil Analisis SPSS

Homogenitas Berat Badan

Test of Homogeneity of Variances

bb_tikus

Statistic Std. Error

bb_tikus Mean 188.47 3.049

95% Confidence Interval for

Mean

Lower Bound 181.93

Upper Bound 195.01

5% Trimmed Mean 188.02

Median 187.00

Variance 139.410

Std. Deviation 11.807

Minimum 172

Maximum 213

Range 41

Interquartile Range 19

Skewness .431 .580

Kurtosis -.463 1.121

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Kolmogorov-Smirnova Shapiro-Wilk

Statistic df Sig. Statistic df Sig.

bb_tikus .124 15 .200* .956 15 .629

*. This is a lower bound of the true significance.

a. Lilliefors Significance Correction

Crosstabs

nekrosis_a * nekrosis_p Crosstabulation

Count

nekrosis_p

Total rendah sedang tinggi sangat tinggi

nekrosis_a rendah 7 1 0 0 8

Errora Approximate Tb

Approximate

Significance

Measure of Agreement Kappa .897 .099 5.343 .000

N of Valid Cases 15

a. Not assuming the null hypothesis.

b. Using the asymptotic standard error assuming the null hypothesis.

Means

Case Processing Summary

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Included Excluded Total

N Percent N Percent N Percent

nekrosis_p * perlakuan 15 100.0% 0 0.0% 15 100.0%

Report

nekrosis_p

perlakuan Mean Std. Deviation Median Minimum Maximum

kontrol negatif 1.20 .447 1.00 rendah sedang

perlakuan Statistic Std. Error

nekrosis_p kontrol negatif Mean 1.20 .200

Mean

Std. Deviation .447

Minimum 1

Maximum 2

Range 1

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Kurtosis 5.000 2.000

kontrol positif Mean 3.20 .200

Mean

Std. Deviation .447

Minimum 3

Maximum 4

Range 1

Skewness 2.236 .913

Kurtosis 5.000 2.000

perlakuan Mean 1.40 .245

Mean

Std. Deviation .548

Minimum 1

Maximum 2

Range 1

Skewness .609 .913

Kurtosis -3.333 2.000

Tests of Normality

perlakuan

Kolmogorov-Smirnova Shapiro-Wilk

Statistic df Sig. Statistic df Sig.

nekrosis_p kontrol negatif .473 5 .001 .552 5 .000

kontrol positif .473 5 .001 .552 5 .000

perlakuan .367 5 .026 .684 5 .006

a. Lilliefors Significance Correction

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Kruskal-Wallis Test

Ranks

perlakuan N Mean Rank

nekrosis_p kontrol negatif 5 5.00

kontrol positif 5 13.00

perlakuan 5 6.00

Total 15

Test Statisticsa,b

nekrosis_p

Chi-Square 10.857

df 2

Asymp. Sig. .004

a. Kruskal Wallis Test

b. Grouping Variable:

perlakuan

Mann-Whitney U .000

Wilcoxon W 15.000

Z -2.785

Asymp. Sig. (2-tailed) .005

Exact Sig. [2*(1-tailed Sig.)] .008b

a. Grouping Variable: perlakuan

b. Not corrected for ties.

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Mann-Whitney Test

Mann-Whitney U 10.000

Wilcoxon W 25.000

Z -.655

b. Not corrected for ties.

NPar Tests

Mann-Whitney U .000

Wilcoxon W 15.000

Z -2.739

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Lampiran 2. Cara kerja sediaan histopatologi

1) Menyiapkan wadah yang di isi dengan larutan formalin 10% buffer dengan

minimal lima kali volume jaringan

2) Testis yang telah diambil, segera dimasukkan ke dalam wadah tersebut

3) Memberi identitas pada semua wadah dengan identitas masing-masing

kelompok perlakuan

4) Dikirim ke Sentra Diagnostik Patologi Anatomi disertai dengan formulir

pengantar

5) Preparat kemudian dipotong dengan ketebalan maksimal 3-4 cm

6) Setelah dipotong diletakkan di dalam kaset jaringan, dan dimasukkan ke

wadah yang berisi formalin 10% buffer

7) Dilakukan proses pembuatan blok parafin, kemudian didinginkan di dalam

lemari es

8) Blok parafin dipotong menjadi lebih tipis menggunakan mikrotom sesuai

kebutuhan

9) Pita parafin dilebarkan dengan ditempelkan langsung pada kaca benda yang

telah dibasahi dengan air

10) Dimulai dengan proses pengecatan Hematoksilin dan Eosin (HE)

11) Perparat diberi cat Hematoksilin

12) Kemudian didiferensiasi dengan menggunakan air kran

13) Diberi cat Eosin

14) Kemudian di dehidrasi menggunakan alkohol 70%

15) Pada proses “clearing” menggunakan larutan xylol

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Lampiran 5. Hasil Pengamatan Nekrosis Sel Hipokampus

Subjek Jumlah Nekrosis (persen) Kategori

Kontrol Negatif 1 6 1

Kontrol Positif 1 51 3

Perlakuan 1 32 2

Perlakuan 2 39 2

Perlakuan 3 12 1

Perlakuan 4 23 1

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