Pengaruh Matriks Hidroksi Propil Metil Selulosa Terhadap Profil Pelepasan In Vitro Salbutamol Dalam Bentuk Tablet Lepas Lambat - Widya Mandala Catholic University Surabaya Repository

(1)

LAMPIRAN A

Hasil Uji Mutu Fisik Granul

Mutu fisik Batch Di

uji Formula Tablet Salbutamol Persyaratan yang diuji FA FB FC FD

I 1 4,67 4,75 4,39 4,3 II 2 4,65 4,73 4,37 4,28

Kadar air III 3 4,69 4,77 4,41 4,32 3-5 (Voigt, 1995)

(persen) X 4,67 475 4,39 4,3

SD 0,02 0,02 0,02 0,02

1 9,69 9,55 9,39 9,11 I 2 9,65 9,53 9,33 9,12 3 9,67 9,52 9,37 9,15 1 9,64 9,55 9,38 9,17

Waktu II 2 9,66 9,58 9,35 9,18

alir (detik) 3 9,63 9,57 9,34 9,11 Tidak lebih dari 1 9,69 9,54 9,38 9,15 10 detik III 2 9,68 9,55 9,36 9,14 (Banker &

3 9,67 9,59 9,37 9,13 Anderson,

X 9,66 9,55 9,36 9,14 1986)

SD 0,02 0,02 0,01 0,02

1 35,62 36,1 36,6 35,33 I 2 34,69 35,24 36,98 35,95 3 35,3 36,18 35,8 35,12 1 34,38 35,19 35,9 36,91

Sudut diam II 2 34,76 36,12 36,87 37,1 25-40 (derajat) 3 35,76 35,87 36,2 35,76 (Banker &

1 35,9 35,9 36,98 36,99 Anderson, III 2 34,1 36,58 35,17 35,13 1986)

3 34,96 36,97 35,67 36,96 X 35,05 36,01 36,15 36,13

SD 0,63 0,56 0,73 0,85

1 13,48 13,78 14,01 13,94 I 2 13,25 13,51 14,62 13,35 3 13,48 13,51 14,01 13,35 1 13,91 13,78 14,62 13,94

Indeks II 2 13,48 13,32 13,5 14,05 11-15 = baik Kompresibilitas 3 13,69 13,32 13,5 14,05

(persen) 1 13,25 13,78 14,79 13,9

III 2 13,48 13,51 13,89 13,35 (Siregar, 1992) 3 13,69 13,78 13,89 13,35

X 13,52 13,58 14,09 13,69

(2)

LAMPIRAN B

Hasil Uji Kekerasan Tablet Salbutamol Batch I

Batch II No

Kekerasan Tablet

Salbutamol

Formula A Formula B Formula C Formula D

1 13,6 13,4 13,9 14,0

2 13,7 14,1 14,1 14,1

3 13,3 13,8 13,9 14,2

4 13,8 13,4 14,2 14,0

5 13,9 13,7 13,8 13,9

6 13,6 14,2 13,9 13,8

7 13,4 13,9 14,1 13,9

8 13,9 14,1 14,3 13,9

9 13,8 13,3 14,0 14,1

10 13,7 13,7 13,9 14,0

X±SD 13,67±0,20 13,76±0,32 14,01±0,15 13,99±0,11

No

Kekerasan Tablet

Salbutamol

1 13,5 13,5 13,7 14,1

2 13,6 13,6 13,8 13,7

3 13,1 13,1 14,0 14,1

4 14,1 14,1 14,1 14,2

5 14,0 14,0 14,1 13,8

6 13,9 13,9 13,9 13,8

7 13,2 13,2 13,8 14,0

8 13,9 12,9 14,1 13,9

9 13,1 13,1 13,7 14,1

10 13,9 12,5 13,9 14,2

(3)

Batch III

No

Kekerasan Tablet

Salbutamol

1 13,7 13,2 13,5 13,9

2 13,5 13,8 13,7 13,8

3 13,4 14,1 13,9 14,1

4 13,7 13,7 14,0 14,2

5 13,1 13,8 14,1 14,1

6 13,9 13,2 14,1 13,9

7 13,7 13,2 13,9 13,9

8 13,0 13,6 13,8 14,1

9 13,3 14,1 14,1 14,2

10 13,8 14,0 14,0 14,0

(4)

LAMPIRAN C

Hasil Uji Kerapuhan Tablet Salbutamol Batch I

Formula Replikasi

Berat awal (gram) Berat akhir (gram) Kerapuhan

(%) X±SD

A

1 5,989 5,978 0,184

2 5,997 5,986 0,183 0,183±0,000215

3 5,983 5,972 0,184

B

1 5,813 5,802 0,189

2 5,815 5,804 0,189 0,189±0,000228

3 5,802 5,791 0,190

C

1 5,997 5,986 0,183

2 5,951 5,940 0,185 0,183±0,001231

3 6,031 6,020 0,182

D

1 6,023 6,012 0,183

2 5,997 5,986 0,183 0,184±0,001341

3 5,938 5,927 0,185

Batch II

Formula Replikasi

Berat awal (gram) Berat akhir (gram) Kerapuhan

(%) X±SD

A

1 5,989 5,978 0,184

2 5,997 5,986 0,183 0,183±0,000215

3 5,983 5,972 0,184

B

1 5,813 5,802 0,189

2 5,815 5,804 0,189 0,189±0,000228

3 5,802 5,791 0,190

C

1 5,997 5,986 0,183

2 5,951 5,940 0,185 0.183±0,001231

3 6,031 6,020 0,182

D

1 6,023 6,012 0,183

2 5,997 5,986 0,183 0,184±0,001341

(5)

Batch III

Formula Replikasi

Berat awal (gram)

Berat akhir (gram)

Kerapuhan

(%) X±SD

A

1 5,989 5,978 0,184

2 5,997 5,986 0,183 0,183±0,000215

3 5,983 5,972 0,184

B

1 5,813 5,802 0,189

2 5,815 5,804 0,189 0,189±0,000228

3 5,802 5,791 0,190

C

1 5,997 5,986 0,183

2 5,951 5,940 0,185 0.183±0,001231

3 6,031 6,020 0,182

D

1 6,023 6,012 0,183

(6)

LAMPIRAN D

Hasil Penetapan Kadar Tablet Lepas Lambat Salbutamol Batch I

Formula Replikasi absorbansi Csampel Cteoritis %kadar X±SD

1 0,045 6,3056 6,2587 100,75 99,75

A 2 0,044 6,1667 6,2442 98,76 ±

3 0,044 6,2222 6,2379 99,75 0,99

1 0,043 6,0278 6,1506 98,00 98,57

B 2 0,044 6,1667 6,2379 98,86 ±

3 0,045 6,3611 6,4355 98,84 0,48

1 0,044 6,1667 6,2587 98,53 98,76

C 2 0,044 6,1667 6,2483 98,69 ±

3 0,045 6,3611 6,4210 99,07 0,27

1 0,044 6,1667 6,1526 100,23 99,82

D 2 0,046 6,4444 6,4022 100,66 ±

3 0,043 6,0833 6,1714 98,57 1,1

Batch II

Formula Replikasi absorbansi Csampel Cteoritis %kadar X±SD

1 0,045 6,3056 6,2566 100,78 100,50

A 2 0,045 6,3056 6,2421 101,02 ±

3 0,044 6,2222 6,2400 99,72 0,69

1 0,043 6,0278 6,1006 98,81 98,87

B 2 0,044 6,1667 6,2421 98,79 ±

3 0,043 6,0833 6,1443 99,01 0,12

1 0,045 6,3056 6,2670 100,61 99,60

C 2 0,043 6,0278 6,0778 99,18 ±

3 0,044 6,2222 6,2837 99,02 0,87

1 0,045 6,3056 6,2483 100,92 100,60

D 2 0,045 6,3056 6,2774 100,45 ±

(7)

Batch III

Formula Replikasi absorbansi Csampel Cteoritis %kadar X±SD

1 0,044 6,1667 6,2171 99,19 100,39

A 2 0,045 6,3056 6,2421 101,02 ±

3 0,046 6,5000 6,4376 100,97 1,04

1 0,045 6,3056 6,2629 100,68 100,25

B 2 0,043 6,0278 6,0798 99,14 ±

3 0,046 6,5000 6,4397 100,94 0,96

1 0,043 6,0278 6,0986 98,84 99,6

C 2 0,045 6,3056 6,2504 100,88 ±

3 0,044 6,2222 6,2795 99,09 1,11

1 0,044 6,1667 6,2587 98,53 99,5

D 2 0,043 6,0278 6,0798 99,14 ±

(8)

LAMPIRAN E

Contoh Perhitungan

Contoh perhitungan sudut diam: Formula A:

W persegi panjang = 4,28 gram W lingkaran = 0,88 gram Luas persegi panjang = 21,5 x 28

= 602 cm2

Luas lingkaran =

602

28

,

4

88

,

0

×

_{= 123,78 cm}2

A = π_.r2

r2 =

A

=

14

,

3

78

,

123

= 39,42

r = 6,28 cm

tg α ₌

r

t

=

28

,

6

5

,

4

= 0,72

α _{= 35,62}°

Contoh perhitungan indeks kompresibilitas: Formula A :

Berat gelas = 125,89 g (W1)

(9)

V2 = 86 ml

Bj nyata = 1 1 2

)

(

V

W

−

=

100

)

89

,

125

69

,

165

(

−

= 0,398

Bj mampat =

2 1 2

)

(

V

W

−

=

87

)

89

,

125

69

,

165

(

−

= 0,46

% kompresibilitas =

x

100

%

mampat

.

Bj

nyata

.

Bj

1

−

= 13,48%

Contoh perhitungan akurasi & presisi:

Absorbansi = 0,044 ( y = 0,0072x – 0,0004 Konsentrasi sebenarnya = 6,1667 ppm Konsentrasi teoritis = 6,2338 ppm

% perolehan kembali = (konsentrasi sebenarnya / konsentrasi teoritis) x 100%

= (6,1667 / 6,2338) x 100% = 98,92 %

Untuk menghitung % KV =

x

100

%

X

SD

=

100

%

99,72

0,93

x

= 0,9331 % % Bahan aktif (mg) Matriks (mg) +Dapar fosfat pH 6,8 ad Pipet +Dapar fosfat pH 6,8 ad Konsent rasi (ppm)

(10)

Contoh perhitungan % obat terlepas:

% obat terlepas =

100

%

100

×

dosis

PK

Wt

Formula A replikasi 1 pada t = 30 menit

% obat terlepas =

100

%

25,02

%

6

,

9

100

100,22

2,4072

=

×

Contoh perhitungan AUC pada disolusi: Rumus: Wtn + W tn-1 x tn – tn-1

2 Formula A batch 1 Wtn-1 = 2,4072 Wtn = 3,1779 tn = 90 menit tn-1 = 60 menit

AUC = ₍₉₀ ₆₀₎

2 1779 , 3 4072 , 2 − + x =83,7765

Luas ) = 360 x penetapan kadar x dosis = 360 x 100,22% x 9,6 mg = 3463,6032

(11)

Perhitungan persamaan orde satu: Rumus: ln (

X

-Ct) = ln C0 - k . t

Dari persamaan regresi ln (

X

-Ct) versus t (waktu), maka didapatkan suatu persamaan regresi dan nilai r, slope serta intersept. Nilai kdiss adalah –slope.

(12)

LAMPIRAN F

Persamaan Formula A Persamaan Orde Nol

r = 0.980345 r tabel = 0,404 Persamaan Higuchi

r = 0,9926 r tabel = 0,404 Persamaan orde satu

r = -0,9793 r tabel = 0,404 0

5 10 15

0 200 400

Formula A

Linear (Formula A)

0 10 20

0 5 10 15 20

Formula A

Linear (Formula A)

-2 0 2 4

0 200 400

Formula A

(13)

LAMPIRAN G

Persamaan Formula B Persamaan orde nol

r = 0,9859 r tabel = 0,404

Persamaan Higuchi

r = 0,9988

r tabel = 0,404 Persamaan orde satu

r = -0,9909 r tabel = 0,404 0

5 10

0 100 200 300 400

Formula B

Linear (Formula B)

0 5 10

0 5 10 15 20

Formula B

Linear (Formula B)

-2 0 2 4

0 100 200 300 400

Formula B

(14)

LAMPIRAN H

Persamaan Formula C Persamaan Orde Nol

r = 0,9826 r tabel = 0,404 Persamaan Higuchi

r = 0,9960 r tabel = 0,404 Persamaan Orde Satu

r = 0,9890 r tabel = 0,404 0

5 10

0 100 200 300 400

Formula C

Linear (Formula C)

0 5 10

0 5 10 15 20

Formula C

Linear (Formula C)

-2 0 2 4

0 100 200 300 400

Formula C

(15)

LAMPIRAN I

Persamaan Formula D Persamaan Orde Nol

r = 0,9769 r tabel = 0,404 Persamaan Higuchi

r = 0,9888 r tabel = 0,404 Persamaan Orde Satu

r = 0,9547 r tabel = 0,404 0

5 10

0 100 200 300 400

Formula D

Linear (Formula D)

0 2 4 6 8

0 5 10 15 20

Formula D

Linear (Formula D)

-1 0 1 2

0 100 200 300 400

Formula D

(16)

LAMPIRAN J

(17)

LAMPIRAN K

(18)

LAMPIRAN L

(19)

LAMPIRAN M

Sertifikat Analisis Magn M

(20)

LAMPIRAN N

(21)

LAMPIRAN O

(22)

LAMPIRAN P

(23)

LAMPIRAN

Tabel Uji r N Q

(24)

LAMPIRAN

Tabel Uji HSD N R

(25)

LAMPIRAN S

Hasil Uji Statistik Kadar Air Antar Formula Anova: Single Factor

SUMMARY

Groups Count Sum Average Variance

Column 1 3 14.01 4.67 0.0004

Column 2 3 14.25 4.75 0.0004

Column 3 3 13.17 4.39 0.0004

Column 4 3 12.9 4.3 0.0004

ANOVA Source of

Variation SS df MS F P-value F crit Between

Groups 0.421425 3 0.140475 3.1875 0.1191 4.0661

Within

Groups 0.0032 8 0.0004

Total 0.424625 11

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

(26)

LAMPIRAN T

Hasil Uji Statistik Waktu AlirAntar Formula Anova: Single Factor

SUMMARY

Column 1 3 29.01 9.67 0.0004

Column 2 3 28.6 9.5333 0.000233

Column 3 3 28.09 9.3633 0.000933

Column 4 3 27.38 9.1266 0.000433

ANOVA Source of

Variation SS df MS F

P-value F crit Between

Groups 0.493667 3 0.1645 3.91111 0.0683 4.0661

Within Groups 0.004 8 0.0005

Total 0.497667 11

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

(27)

LAMPIRAN U

Hasil Uji Indeks Kompresibilitas Antar Formula Anova: Single Factor

SUMMARY

Column 1 3 105.61 35.203 0.223233

Column 2 3 107.52 35.84 0.2716

Column 3 3 109.38 36.46 0.3628

Column 4 3 106.4 35.466 0.186233

ANOVA Source of

Groups 2.673292 3 0.8910 3.414602 0.0732 4.0661

Within

Groups 2.087733 8 0.2609

Total 4.761025 11

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

(28)

LAMPIRAN V

Hasil Uji Statistik Sudut Diam Antar Formula Anova: Single

Factor

SUMMARY

Column 1 3 40.21 13.40333 0.017633

Column 2 3 40.8 13.6 0.0243

Column 3 3 42.64 14.21333 0.124033

Column 4 3 40.64 13.54667 0.116033

ANOVA

Source of Variation SS df MS F

P-value F crit Between Groups 1.154092 3 0.384697 3.456698 0.0695 4.0661

Total 1.718092 11

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

(29)

LAMPIRAN W

Hasil Uji Statistik Kekerasan Tablet Formula A Antar Batch

Anova: Single Factor

SUMMARY

Column 1 10 136.7 13.67 0.040111

Column 2 10 136.3 13.63 0.149

Column 3 10 135.1 13.51 0.092111

ANOVA Source of

Within Groups 2.531 27 0.093741

Total 2.669667 29

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

(30)

LAMPIRAN X

Hasil Uji Statistik Kekerasan Tablet Formula B Antar Batch

SUMMARY

Column 1 10 137.6 13.76 0.102667

Column 2 10 133.9 13.39 0.269889

Column 3 10 136.7 13.67 0.131222

ANOVA Source of

Within Groups 4.534 27 0.167926

Total 5.278667 29

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

(31)

LAMPIRAN Y

Hasil Uji Statistik Kekerasan Tablet Formula C Antar Batch

SUMMARY

Column 1 10 140.1 14.01 0.025444

Column 2 10 139.1 13.91 0.025444

Column 3 10 139.1 13.91 0.038778

ANOVA Source of

Within Groups 0.807 27 0.029889

Total 0.8736667 29

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

(32)

LAMPIRAN Z

Hasil Uji Statistik Kekerasan Tablet Formula D Antar Batch

SUMMARY

Column 1 10 139.9 13.99 0.014333

Column 2 10 139.9 13.99 0.032111

Column 3 10 140.2 14.02 0.019556

ANOVA Source of

Total 0.6 29

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

(33)

LAMPIRAN AA

Hasil Uji Statistik Kekerasan Tablet Antar Formula Batch 1

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

" ! "

SUMMARY

Column 1 10 136.7 13.67 0.040111

Column 2 10 137.6 13.76 0.102667

Column 3 10 140.1 14.01 0.025444

Column 4 10 139.9 13.99 0.014333

ANOVA Source of

Groups 0.85475 3 0.284917 2.242848 0.0715 2.8662

Within

Groups 1.643 36 0.045639

(34)

LAMPIRAN AB

Hasil Uji Statistik Kekerasan Tablet Antar Formula Batch 2

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

" ! "

SUMMARY

Column 1 10 136.3 13.63 0.149

Column 2 10 133.9 13.39 0.269889

Column 3 10 139.1 13.91 0.025444

Column 4 10 139.9 13.99 0.032111

ANOVA Source of

Variation SS df MS F P-value F crit Between Groups 2.256 3 0.752 2.313433 0.07149 2.8662 Within Groups 4.288 36 0.119111

(35)

LAMPIRAN AC

Hasil Uji Statistik Kekerasan Tablet Antar Formula Batch 3

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

" ! "

SUMMARY

Column 1 10 135.1 13.51 0.092111

Column 2 10 136.7 13.67 0.131222

Column 3 10 139.1 13.91 0.038778

Column 4 10 140.2 14.02 0.019556

ANOVA Source of

Groups 1.59475 3 0.531583 2.549112 0.0704 2.8662

Within Groups 2.535 36 0.070417

(36)

LAMPIRAN AD

Hasil Uji Statistik Kerapuhan Tablet Formula A Antar Batch

SUMMARY

Column 1 3 0.567987 0.189328 5.21E-08

Column 2 3 0.567987 0.189328 5.21E-08

Column 3 3 0.567987 0.189328 5.21E-08

ANOVA Source of

Groups 0 2

-8.47E-22 -5.6E-16 1 5.1432

Within Groups

3.1259E-07 6

5.2099E-08

Total

3.1259E-07 8

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

(37)

LAMPIRAN AE

Hasil Uji Statistik Kerapuhan Tablet Formula B Antar Batch

SUMMARY

Column 1 3 0.5679 0.189328 5.21E-08

Column 2 3 0.5679 0.189328 5.21E-08

Column 3 3 0.5679 0.189328 5.21E-08

ANOVA Source of

Groups 0 2

-8.47E-22 -5.6E-16 1 5.1432 Within

Groups

3.1259E-07 6

5.2099E-08

Total

3.1259E-07 8

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

(38)

LAMPIRAN AF

Hasil Uji Statistik Kerapuhan Tablet Formula C Antar Batch

SUMMARY

Groups Count Sum Average Variance Column 1 3 0.550659 0.183553 1.52E-06 Column 2 3 0.550659 0.183553 1.52E-06 Column 3 3 0.550659 0.183553 1.52E-06

ANOVA Source of

Groups

-1.7E-21 2 -8.5E-22 -5.6E-16 1 5.1432

Within Groups

9.09E-06 6 1.52E-06

Total

9.09E-06 8

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

(39)

LAMPIRAN AG

Hasil Uji Statistik Kerapuhan Tablet Formula D Antar Batch

SUMMARY

Column 1 3 0.5513 0.183769 1.8E-06

Column 2 3 0.5513 0.183769 1.8E-06

Column 3 3 0.5513 0.183769 1.8E-06

ANOVA Source of

Groups 1.69E-21 2 8.47E-22

4.71E-16 1 5.1432

Within

Groups 1.08E-05 6 1.8E-06

Total 1.08E-05 8

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

(40)

LAMPIRAN AH

Hasil Uji Statistik Kerapuhan Tablet Antar Formula Batch 1 Anova: Single Factor

SUMMARY

Column 1 3 0.550949 0.18365 4.64E-08

Column 2 3 0.567987 0.189329 5.21E-08

Column 3 3 0.550659 0.183553 1.52E-06

Column 4 3 0.551306 0.183769 1.8E-06

ANOVA Source of

Groups

7.25E-05 3 2.42E-05 2.321472 0.070 4.0661

Within Groups

6.82E-06 8 8.53E-07

Total

7.93E-05 11

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

(41)

LAMPIRAN AI

SUMMARY

Column 1 3 0.550949 0.18365 4.64E-08

Column 2 3 0.567987 0.189329 5.21E-08

Column 3 3 0.550659 0.183553 1.52E-06

Column 4 3 0.551306 0.183769 1.8E-06

ANOVA Source of

Variation SS df MS F P-value F crit Between

Groups

7.25E-05 3 2.42E-05 2.321472 0.070 4.0661

Within Groups

6.82E-06 8 8.53E-07

Total

7.93E-05 11

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

(42)

LAMPIRAN AJ

SUMMARY

Column 1 3 0.550949 0.18365 4.64E-08

Column 2 3 0.567987 0.189329 5.21E-08

Column 3 3 0.550659 0.183553 1.52E-06

Column 4 3 0.551306 0.183769 1.8E-06

ANOVA Source of

Groups 7.25E-05 3 2.42E-05 2.321472 0.070 4.0661

Within

Groups 6.82E-06 8 8.53E-07

Total 7.93E-05 11

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

(43)

LAMPIRAN AK

Hasil Uji Statistik Penetapan Kadar Tablet Formula A Antar Batch

SUMMARY

Column 1 3 299.2556 99.75187 0.989415

Column 2 3 301.5138 100.5046 0.481305

Column 3 3 301.1747 100.3916 1.086122

ANOVA Source of

Groups 0.988583 2 0.494291 0.579963 0.5884 5.1432

Within

Groups 5.113684 6 0.852281

Total 6.102267 8

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

(44)

LAMPIRAN AL

Hasil Uji Statistik Penetapan Kadar Tablet Formula B Antar Batch

SUMMARY

Column 1 3 295.7052 98.56841 0.239205

Column 2 3 296.6049 98.86832 0.014562

Column 3 3 300.7618 100.2539 0.940765

ANOVA Source of

Groups 4.85083 2 2.425415 5.091291 0.0759 5.1432

Within

Groups 2.389065 6 0.398177

Total 7.239895 8

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

(45)

LAMPIRAN AM

Hasil Uji Statistik Penetapan Kadar Tablet Formula C Antar Batch

SUMMARY

Column 1 3 296.2903 98.76344 0.076254

Column 2 3 298.8141 99.60471 0.770921

Column 3 3 298.8093 99.60311 1.242892

ANOVA Source of

Groups 1.412808 2 0.706404 1.013945 0.4174 5.1432

Within

Groups 4.180134 6 0.696689

Total 5.592943 8

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

(46)

LAMPIRAN AN

Hasil Uji Statistik Penetapan Kadar Tablet Formula D Antar Batch

SUMMARY

Column 1 3 299.4608 99.82027 1.212087

Column 2 3 301.8156 100.6052 0.072452

Column 3 3 298.5046 99.50154 1.42146

ANOVA Source of

Groups 1.935818 2 0.967909 1.073071 0.3995 5.1432

Within

Groups 5.411996 6 0.901999

Total 7.347814 8

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

(47)

LAMPIRAN AO

Hasil Uji Statistik Penetapan Kadar Tablet Antar Formula Batch 1 Anova: Single

Factor

SUMMARY

Column 1 3 299.2556 99.75187 0.989415

Column 2 3 295.7052 98.56841 0.239205

Column 3 3 296.2903 98.76344 0.076254

Column 4 3 299.4608 99.82027 1.212087

ANOVA Source of

Groups 3.828267 3 1.276089 2.027984 0.188 4.0661

Within

Groups 5.033922 8 0.62924

Total 8.862188 11

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

(48)

LAMPIRAN AP

Hasil Uji Statistik Penetapan Kadar Tablet Antar Formula Batch 2 Anova: Single Factor

SUMMARY

Column 1 3 301.5138 100.5046 0.481305

Column 2 3 296.6049 98.86832 0.014562

Column 3 3 298.8141 99.60471 0.770921

Column 4 3 301.8156 100.6052 0.072452

ANOVA Source of

Groups 6.043101 3 2.014367 4.016449 0.0693 4.0661

Within

Groups 2.678479 8 0.33481

Total 8.72158 11

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

(49)

LAMPIRAN AQ

Hasil Uji Statistik Penetapan Kadar Tablet Antar Formula Batch 3 Anova: Single Factor

SUMMARY

Column 1 3 301.1747 100.3916 1.086122

Column 2 3 300.7618 100.2539 0.940765

Column 3 3 298.8093 99.60311 1.242892

Column 4 3 299.6354 99.87848 0.748171

ANOVA Source of

Groups 1.158149 3 0.38605 0.384325 0.7673 4.0661

Within

Groups 8.035901 8 1.004488

Total 9.19405 11

! "

! # $

% &

' $ ( ) *+ , ,

- # # $ . # $ , ,

(50)

LAMPIRAN AR

Hasil Uji Statistik % ED360

PERHITUNGAN ANAVA (Uji Statistik % ED360 ) Anova: Single Factor

SUMMARY

Column 1 3 196.26 65.421 0.3150 Column 2 3 155.126 51.708 0.001

Column 3 3 142.2 47.423 0.0126

Column 4 3 117.914 39.304 0.0069

ANOVA

Source of

P-value F crit

Between

Groups 1074.162 3 358.054 4265.423 3.7E-13 4.066 Within

Groups 0.671547 8 0.08394

Total 1074.834 11

keterangan: F hitung>F tabel (0,05) sehingga H ditolak dan ada perbedaan yang bermakna antar formula

HSD = 0.6562

FA FB FC F D

Mean 65.4216 51.7088 47.423 39.3047

formula A 65.4216 0 13.71278 * 17.998 * 26.1169 *

formula B 51.7088 0 4.2855 * 12.4041 *

formula C 47.4233 0 8.11861 *

(51)

LAMPIRAN AS

Hasil Uji Statistik % Obat Terlepas Anova:

Single Factor

SUMMAR Y

Column 1 3 298.48 99.493 0.1980

Column 2 3 256.16 85.387 0.1758

Column 3 3 240.59 80.197 0.0037

Column 4 3 210.58 70.193 0.0058

ANOVA

Source of

P-value F crit

Between

Groups 1340.767 3 446.92 4662.322 3E-13 4.066181 Within

Groups 0.766867 8 0.0959

Total 1341.534 11

keterangan: F hitung>F tabel (0,05) sehingga H ditolak dan ada perbedaan yang bermakna antar formula

HSD = 0.701267

FA F B F C FD

Mean 99.493 85.386 80.196 70.19333

formula A 99.493 0 14.106 * 19.296 * 29.3 *

formula B 85.386 0 5.19 * 15.19333 *

formula C 80.196 0 10.00333 *

Formula D 70.193

Keterangan

(52)

LAMPIRAN AT

Uji F Kurva Baku REPLIKASI 1

KONSENTRASI ABSORBANSI X2 Y2 XY

1.53 0.011 2.331 0.000121 0.0168

4.07 0.029 16.581 0.000841 0.1181 6.11 0.044 37.307 0.001936 0.2688 8.14 0.054 66.324 0.002916 0.4398 10.18 0.075 103.632 0.005625 0.7635 30.03 0.213 226.177 0.011439 1.6069

REPLIKASI 2

KONSENTRASI ABSORBANSI X2 _Y2 _XY

1.52 0.012 2.295 0.000144 0.0182

4.04 0.028 16.321 0.000784 0.1131 6.06 0.042 36.723 0.001764 0.2545 8.08 0.052 65.286 0.002704 0.4202 10.10 0.074 102.010 0.005476 0.7474 29.80 0.208 222.636 0.010872 1.5534

REPLIKASI 3

KONSENTRASI ABSORBANSI X2 Y2 XY

1.54 0.014 2.359 0.000196 0.0215

4.10 0.033 16.777 0.001089 0.1352

6.14 0.049 37.748 0.002401 0.3011 8.19 0.057 67.108 0.003249 0.4669 10.24 0.079 104.857 0.006241 0.8090 30.21 0.232 228.851 0.013176 1.7336

Σ_X2 _Σ

XY Σ_Y2 _N _SSi _RDF

Regresi I 226.1777 1.606 0.011439 5 0.0043 4 Regresi II 222.6368 1.553 0.010872 5 0.0039 4 Regresi III 228.8517 1.733 0.013176 5 0.0056 4

677.6663 4.893 0.035487 0.0138

SSc= 0.028265 267 F=

3.2929

(53)

LAMPIRAN AU

LOD dan LOQ

y y- (y- )2

0.011 0.01128 -0.00028 0.0000001 LOD y=yB+3SB= -0.0003

0.029 0.02948 -0.00048 0.0000002 X= 0.006

0.044 0.04404 -0.00004 0.0000000

0.054 0.05860 -0.00460 0.0000212 LOQ y=yB+10SB= -0.0002

0.075 0.07316 0.00184 0.0000034 X= 0.0201

(y- )2 0.0000248

SB 0.0000143

y y- (y- )2

0.012 0.01043 0.00157 0.0000025 LOD y=yB+3SB= 0.0002

0.028 0.02801 -0.00001 0.0000000 X= 0.008

0.042 0.04208 -0.00008 0.0000000

0.052 0.05614 -0.00414 0.0000171 LOQ y=yB+10SB= 0.0003

0.074 0.07020 0.00380 0.0000144 X= 0.0282

(y- )2 0.0000340

SB 0.0000196

y y- (y- )2

0.014 0.00788 0.00612 0.0000375 LOD y=yB+3SB= 0.0035

0.033 0.02621 0.00679 0.0000460 X= 0.055

0.049 0.04088 0.00812 0.0000659

0.057 0.05555 0.00145 0.0000021 LOQ y=yB+10SB= 0.0044

0.079 0.07022 0.00878 0.0000770 X= 0.1842

(y- )2 0.0002285

SB 0.0001319

Rata-rata

LOD= 0.023 ppm